breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 708,647 G→T S105I (AGC→ATC)  chiP → chitobiose outer membrane channel
RA 842,105 G→T intergenic (‑49/‑227) mcbA ← / → rlmF DUF1471 domain‑containing protein McbA/23S rRNA m(6)A1618 methyltransferase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,144,615 C→A P38P (CCC→CCA yceQ → DUF2655 domain‑containing protein YceQ
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,426,070 G→T intergenic (+90/‑384) ydaY → / → ynaA Rac prophage; putative uncharacterized protein YdaY/Rac prophage; putative prophage tail length tape measure domain‑containing protein YnaA
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,976,810 G→T F110L (TTC→TTA motA ← motility protein A
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,244,751 C→A intergenic (+5/+27) yeiG → / ← cirA S‑formylglutathione hydrolase/S‑lactoylglutathione hydrolase/ferric dihyroxybenzoylserine outer membrane transporter
RA 2,261,926 G→T P147Q (CCG→CAG)  fruK ← 1‑phosphofructokinase
RA 2,316,150 G→T S888I (AGC→ATC)  rcsD → RcsD phosphotransferase
RA 2,382,676 C→A intergenic (+151/‑37) rbn → / → elaD ribonuclease BN/protease ElaD
RA 2,436,238 G→T L138M (CTG→ATG)  usg ← putative semialdehyde dehydrogenase Usg
RA 2,448,272 G→T L57M (CTG→ATG)  prmB ← 50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase
RA 2,690,087 G→T H338N (CAT→AAT)  glrK ← sensory histidine kinase GlrK
RA 2,851,992 C→A R43R (CGC→CGA hypC → hydrogenase 3 maturation protein HypC
RA 2,981,635 A→C V183G (GTT→GGT)  araE ← arabinose:H(+) symporter
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,420,478 G→T S115S (TCG→TCT yhdX → putative ABC transporter membrane subunit YhdX
RA 3,442,139 A→C G111G (GGT→GGG rpsM ← 30S ribosomal subunit protein S13
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,604,013 C→A Q77H (CAG→CAT ftsY ← signal recognition particle receptor
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,100,288 C→A G80C (GGC→TGC)  rhaT ← rhamnose/lyxose:H(+) symporter
RA 4,171,557 C→G intergenic (+13/‑80) rrlB → / → rrfB 23S ribosomal RNA/5S ribosomal RNA
RA 4,215,491 C→A A5E (GCA→GAA)  aceB → malate synthase A
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102960 3104047 1088 14 [13] [13] 14 [mutY] [mutY]
* * ÷ NC_000913 3423765–3424234 3424525–3424238 5–761 14 [12] [13] 16 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =21 (0.340)58 (1.050) 42/244 0.2 66.8% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 39 (0.700)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780521 (0.340)43 (0.780) 34/244 0.4 59.9% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 39 (0.700)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 3363001 =90 (1.440)78 (1.260) 44/274 0.2 63.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)83 (1.330) 60/276 0.0 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 0 (0.000)coding (3907/4134 nt) rhsA rhs element protein RhsA