breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 264,098 G→T 100% L50L (CTC→CTA ykfH ← uncharacterized protein
RA 275,065 G→A 100% T18I (ACC→ATC)  insH1 ← IS5 transposase and trans‑activator
RA 333,465 A→G 5.7% intergenic (+6/+36) yahA → / ← yahB c‑di‑GMP‑specific phosphodiesterase/putative DNA‑bindng transcriptional regulator
RA 372,055 C→A 6.8% H273N (CAT→AAT)  mhpC → 2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase
RA 482,230 G→A 100% P725L (CCG→CTG)  acrB ← multidrug efflux system protein
RA 871,104 G→T 8.0% Q46H (CAG→CAT gsiC → glutathione ABC transporter permease
RA 1,026,965 C→G 12.2% V17V (GTG→GTC mgsA ← methylglyoxal synthase
RA 1,051,638 C→T 9.4% R60C (CGT→TGT)  cspG → cold shock protein homolog, cold‑inducible
RA 1,197,502 C→T 100% G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
RA 1,494,281 G→T 7.3% A264D (GCC→GAC)  ydcI ← putative DNA‑binding transcriptional regulator
RA 1,544,022 1 bp→AA 100% coding (39/1389 nt) narU ← nitrate/nitrite transporter
RA 1,933,131 G→A 8.9% P492S (CCG→TCG)  edd ← 6‑phosphogluconate dehydratase
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,106,741 A→T 100% S162S (TCT→TCA wbbH ← O‑antigen polymerase
RA 2,172,505 A→T 6.2% V123V (GTT→GTA gatD ← galactitol‑1‑phosphate dehydrogenase, Zn‑dependent and NAD(P)‑binding
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,355,843 T→C 9.4% F108S (TTC→TCC)  glpC → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster
RA 2,384,151 C→T 7.4% G524G (GGG→GGA yfbK ← Von Willebrand factor domain putative lipoprotein
RA 2,391,417 C→G 8.3% W30S (TGG→TCG)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N
RA 2,679,525 G→T 100% A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,795,396 C→A 100% Q382K (CAG→AAG)  gabP → gamma‑aminobutyrate transporter
RA 2,817,170 C→A 100% E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase
RA 3,440,923 C→T 49.7% G36D (GGC→GAC)  rpoA ← RNA polymerase, alpha subunit
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,591,716 A→G 7.6% W204R (TGG→CGG)  ugpB ← sn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein
RA 3,698,796 C→T 9.0% P195S (CCC→TCC)  bcsG → DUF3260 family cellulose production inner membrane protein
RA 4,032,422 G→A 5.7% R421H (CGT→CAT)  pepQ → proline dipeptidase
RA 4,111,596 G→A 100% L179L (CTG→TTG)  yiiQ ← DUF1454 family putative periplasmic protein
RA 4,186,457 A→G 45.3% K398E (AAG→GAG)  rpoC → RNA polymerase, beta prime subunit
RA 4,295,976 C→T 21.7% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,296:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,545,683 C→A 7.8% D224E (GAC→GAA fimD → fimbrial usher outer membrane porin protein; FimCD chaperone‑usher
RA 4,604,632 C→A 100% A186E (GCA→GAA)  bglJ → bgl operon transcriptional activator

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 5565 =NA (NA)3 (0.060) 3/258 NT NA noncoding (1/105 nt) RIP1 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP1 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913_3_pae_tpiA 5597 = NA (NA)noncoding (33/105 nt) RIP1 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP1 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913_3_pae_tpiA 948822 =36 (0.690)4 (0.080) 4/258 NT 10.8% coding (747/909 nt) ycaN LysR family putative transcriptional regulator
?NC_000913_3_pae_tpiA 948840 = 32 (0.650)coding (729/909 nt) ycaN LysR family putative transcriptional regulator
* ? NC_000913_3_pae_tpiA 1207790 =4 (0.080)33 (0.720) 30/240 NT 84.0% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_pae_tpiA 1209619 = 9 (0.200)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_pae_tpiA = 12078053 (0.060)38 (0.830) 31/240 NT 86.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_pae_tpiA = 1209602 9 (0.200)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)39 (0.770) 32/264 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913_3_pae_tpiA 2769787 =67 (1.290)3 (0.060) 3/252 NT 5.1% coding (85/702 nt) yfjR CP4‑57 prophage; putative DNA‑binding transcriptional regulator
?NC_000913_3_pae_tpiA 2769834 = 50 (1.040)coding (132/702 nt) yfjR CP4‑57 prophage; putative DNA‑binding transcriptional regulator
* ? NC_000913_3_pae_tpiA 4122239 =55 (1.060)3 (0.060) 3/256 NT 5.5% intergenic (‑36/+57) hslV/ftsN peptidase component of the HslUV protease/essential cell division protein
?NC_000913_3_pae_tpiA 4122259 = 52 (1.060)intergenic (‑56/+37) hslV/ftsN peptidase component of the HslUV protease/essential cell division protein