Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A134 F1 I1 R1
|
15 |
220.5 |
13917488 |
95.6% |
13305118 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,131,466 |
(A)9→8 |
intergenic (‑125/+175) |
gatY ← / ← fbaB |
D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,131,458 | 0 | A | . | 99.0%
| 522.6
/ ‑3.0
| 103 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (84/18); total (84/19) |
| Fisher's exact test for biased strand distribution p-value = 1.84e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 149 total aligned reads displayed.
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG > REL606/2131383‑2131533
|
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 1:2242656/1‑76 (MQ=255)
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 1:6888735/1‑76 (MQ=255)
gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 1:2371875/1‑76 (MQ=255)
gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 1:416419/1‑76 (MQ=255)
gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa < 2:5581297/76‑1 (MQ=255)
aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa < 2:2470524/76‑1 (MQ=255)
tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 2:5833247/76‑1 (MQ=255)
tGTTTTTAAATGTCGTGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:153041/1‑75 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:5821644/1‑75 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac < 2:5386175/76‑2 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 2:3219438/1‑75 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:5701496/1‑75 (MQ=255)
gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg > 2:3478112/1‑74 (MQ=255)
gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg > 1:3505863/1‑74 (MQ=255)
tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 2:3090890/1‑76 (MQ=255)
tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 1:150697/1‑76 (MQ=255)
tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 1:5649532/1‑76 (MQ=255)
ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 1:6067660/1‑76 (MQ=255)
ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:4000246/1‑76 (MQ=255)
ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 1:3375804/1‑76 (MQ=255)
ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 1:3313853/1‑76 (MQ=255)
ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:488618/1‑76 (MQ=255)
tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc > 2:5330975/1‑76 (MQ=255)
tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc < 2:5503404/76‑1 (MQ=255)
tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc > 2:355597/1‑76 (MQ=255)
aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc < 2:6489362/76‑1 (MQ=255)
aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:5855007/1‑76 (MQ=255)
aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 2:6810369/1‑76 (MQ=255)
aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 1:5555507/1‑76 (MQ=255)
aTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑g < 2:617978/76‑1 (MQ=255)
tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑gg < 2:2432494/76‑1 (MQ=255)
cgTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑gg > 2:2850491/2‑76 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 1:1964909/1‑76 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 1:4985687/1‑76 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 1:2037111/1‑76 (MQ=255)
cGAGATAACGAGCACAAAAACGACAATATGCAAATTAATAGAGGAACGAAAGGC‑AAAAAAATCGGCCTCCC‑GGacg < 2:2713648/76‑3 (MQ=255)
gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAggg < 2:6684161/76‑1 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGa > 2:5664133/1‑76 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGa < 1:100847/76‑1 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGa > 1:2712415/1‑76 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGa < 1:4045778/76‑1 (MQ=255)
gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGaa > 2:6144784/1‑76 (MQ=255)
gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGaa < 1:3077192/76‑1 (MQ=255)
aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAg < 1:1428588/76‑1 (MQ=255)
aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAg < 1:6868676/76‑1 (MQ=255)
aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAg > 2:6338232/1‑76 (MQ=255)
tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGc > 1:521702/1‑76 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGcc > 2:4616716/1‑76 (MQ=255)
aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCg > 1:1526321/1‑76 (MQ=255)
cGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGc > 1:6552441/1‑76 (MQ=255)
cGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGc > 1:3194286/1‑76 (MQ=255)
cGATCAAAAAAACGACAATATAAAAATAAATAGAGGAATAAAAGG‑AAAAAAAAACGGCCCCCC‑GGAGGAAAGCCGc < 2:3035771/76‑1 (MQ=255)
gATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGca > 1:6693797/1‑76 (MQ=255)
tCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGcaac > 1:6352830/1‑76 (MQ=255)
cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGcaaca > 2:670825/1‑76 (MQ=255)
acaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGcaacaa > 2:4401480/1‑76 (MQ=255)
aaaaaaCGAAAATATAAAAATAAAAAGAGGAAGGAAAGG‑AAAAAAAACCGCCCCCCC‑GGGGGAAAGCCGCAACaaa < 2:167103/75‑1 (MQ=255)
aaaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCAGCAACAAAg > 2:4647129/1‑76 (MQ=255)
aaaaCGACAATATGAAAATTAATAGAGGAATAAAAGGC‑AAAAAAAACGGCCCCCC‑GGAGGGAAGCCGCAACAAAGt < 1:5427186/76‑1 (MQ=255)
aaaaCGACAATATGAAAATTAATAGAGAAATGAAAGG‑AAAAAAAACCGGCCCCCC‑GGAGGGAAGCCGCAACAAAGt < 1:4548055/76‑1 (MQ=255)
aaaaCAAAAAAATAAAAATTAAAAGAGAAAT‑AAAAGAAAAAAAAACCGCCCCCCC‑GGGGGAAAGCCGCAACAAAGt < 2:4859588/76‑1 (MQ=255)
aaaaCAAAAAAATAAAAAACAATAGAGG‑ATGAAAAAAAAAAAAAAGCGCCCCCCG‑GGAGGGAAGCCGCAACAAAGt < 2:4693052/76‑1 (MQ=255)
aaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTg < 1:1728545/76‑1 (MQ=255)
aCGACAATATGAAAATTAATAGAGGAATAAAAGG‑AAAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTgcg < 1:5006492/76‑1 (MQ=255)
cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTgcgc > 2:2539894/1‑76 (MQ=255)
aCAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGt > 2:3210635/1‑76 (MQ=255)
cAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTg > 2:4907203/1‑76 (MQ=255)
cAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTg > 2:2052155/1‑76 (MQ=255)
cAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTg > 1:4227476/1‑76 (MQ=255)
aaTATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTgg > 1:3702220/1‑76 (MQ=255)
aaTATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTgg > 2:1014086/1‑76 (MQ=255)
atatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGt > 2:2584790/1‑76 (MQ=255)
tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGtt > 1:6209377/1‑76 (MQ=255)
tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGtt > 2:4186025/1‑76 (MQ=255)
atGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGttt > 1:1015777/1‑76 (MQ=255)
tGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGtttt > 2:1434088/1‑76 (MQ=255)
aaaaaaTAAATAGA‑GAAAGAAAAGAAAAAAAAACCGCCCCCCG‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGtttt < 2:698293/74‑1 (MQ=255)
aaaaTTAAGAGGGGAATGAAA‑GAAAAAAAAACCGCCCCCCC‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 2:897410/76‑1 (MQ=255)
aaaTTAATAGAGAAATAAAAGGC‑AAAAAAAACGGCCCCCC‑GGGGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATg < 1:1602962/76‑1 (MQ=255)
aaaTAAAGAGAGGAATG‑AAGGAAAAAAAAAACGCCCCCCC‑GGGGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATg < 2:1494751/76‑1 (MQ=255)
aaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGc > 2:2114372/1‑76 (MQ=255)
aTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa > 1:2237922/1‑76 (MQ=255)
aTTAAGAGAGAAAGGAAAGG‑AAAAAAAACCGCCCCCCG‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa < 2:5535336/76‑1 (MQ=255)
ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 2:396707/1‑76 (MQ=255)
ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 1:82480/1‑76 (MQ=255)
aaagagGAATAAAAGGC‑AAAAAAAACGGCCCCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt < 1:1313493/74‑1 (MQ=255)
aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt > 2:6855256/1‑76 (MQ=255)
tAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg > 1:1004582/1‑76 (MQ=255)
aagagAAATAAAAGG‑AAAAAAAACCGCCCCCCC‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg < 2:1090839/75‑1 (MQ=255)
agagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 2:1276038/1‑76 (MQ=255)
gagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcg > 1:731577/1‑76 (MQ=255)
gAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg > 1:6336025/1‑76 (MQ=255)
gAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg > 1:1666046/1‑76 (MQ=255)
aaTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt > 1:4771751/1‑76 (MQ=255)
aaTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt > 2:1533804/1‑76 (MQ=255)
aTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa > 2:5897029/1‑76 (MQ=255)
aTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGGGGTTTTATGCAGGATGCGGCGTa > 1:3991159/1‑76 (MQ=255)
tGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 1:5210230/1‑76 (MQ=255)
aaGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACa > 2:5474878/1‑76 (MQ=255)
gaaaaaaaaaCCGCCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACata < 2:5658111/75‑1 (MQ=25)
|
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG > REL606/2131383‑2131533
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
