Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
20 |
51.0 |
3438118 |
91.8% |
3156192 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,131,466 |
(A)9→8 |
intergenic (‑125/+175) |
gatY ← / ← fbaB |
D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,131,458 | 0 | A | . | 92.1%
| 154.8
/ 7.9
| 38 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base . (29/6); total (32/6) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATAT > REL606/2131383‑2131532
|
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa < 1:334083/76‑1 (MQ=255)
aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa > 2:1466197/1‑76 (MQ=255)
gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATTAAAGGCaaaaa < 1:56373/76‑1 (MQ=255)
gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 1:817382/1‑76 (MQ=255)
gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 1:936083/1‑76 (MQ=255)
aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 1:335851/1‑76 (MQ=255)
tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa > 1:399245/1‑76 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac < 1:754941/76‑2 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:1193738/1‑75 (MQ=255)
gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg > 1:1025385/1‑74 (MQ=255)
gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg < 2:400837/76‑3 (MQ=255)
ttttAAATGTCGAGATANCGATCACAAAAACGACAATATGAAAATTAATAGATGAATGAAAGG‑AAACAAAAACGGc > 1:214132/1‑76 (MQ=255)
ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 1:1099278/1‑76 (MQ=255)
tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc > 1:119153/1‑76 (MQ=255)
aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTcc > 2:1254343/1‑76 (MQ=255)
gTCGAGATAACGATCACTAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa > 1:1697986/1‑76 (MQ=255)
gTCGAGATAACGATCACAAAAACGACAATATGAAGATTAATNGAGGAATGAAAGGC‑AAAANAANCGGCCTCCCGGa > 1:1396242/1‑76 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 2:45820/1‑76 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAAATAATAGNGGAATGAAAGGC‑AAAAAANACNGCCTCCCGGAgg > 1:1406798/1‑76 (MQ=255)
cGAGATAACGACCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 1:98362/1‑76 (MQ=255)
gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAggg < 2:1063147/76‑1 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATCGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 1:1440738/1‑76 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 2:1131878/1‑76 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa < 2:1046643/76‑1 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa < 2:817381/76‑1 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa < 2:971132/76‑1 (MQ=255)
agaTAACGATCACAAAAACGACAATATAAAAATTCATAGAGGAAAGAANCG‑ATAAAAAAACGGCCTCCCGGAGGGa < 2:9468/76‑1 (MQ=255)
acctAACGCTCACAAAAACTACACTATCAAAATTAATAGATGAAT‑AAATGTCTAAAAAAACGGCCTCCCTTAGGGa > 2:17349/4‑76 (MQ=255)
gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGaa < 2:1547185/76‑1 (MQ=255)
aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAg > 1:1280288/1‑76 (MQ=255)
tAACGTTCACAAAAACGACAATATGAAAATTAATAGAGGAACGAAAGGC‑AAAAANAACGGCCTGCNGGATGGAAGc < 2:1682733/76‑1 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc > 1:662720/1‑76 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc > 2:549362/1‑76 (MQ=255)
aaaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAg > 2:1352547/1‑76 (MQ=255)
aaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgc > 2:675760/1‑76 (MQ=255)
cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgcgc > 2:582512/1‑76 (MQ=255)
tataAAAATTAAGAGAGGAA‑GAAAAGCAAAAAAAACCGCCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtt < 2:1469331/76‑1 (MQ=255)
tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtt > 1:1055381/1‑76 (MQ=255)
tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtt > 1:1344928/1‑76 (MQ=255)
tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtt > 1:378999/1‑76 (MQ=255)
tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGCAAGCCGCAACAAAGTGCGCGTGGtg > 1:263519/1‑75 (MQ=255)
atNAAAATTAATAGAGGAATGAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGAntt < 2:1394016/76‑1 (MQ=255)
gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa > 2:1025657/1‑76 (MQ=255)
aaaaTTAATAGAGAAATGAAAGAC‑ANATAAACCGGCATCCCGGAGGGNAGCAGCAACAAAGTGCGCGTGGTTTTAt < 1:239872/76‑1 (MQ=255)
aaTTACTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGc > 1:111642/1‑76 (MQ=255)
tAATAGAGGAATGAAAGGCACAAAAAAA‑GGCCTCCCGGAGGGAAGCCGCAACAGAGTGCGCGTGGTTTTATGCAgg > 2:132540/1‑76 (MQ=255)
tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:421872/1‑76 (MQ=255)
tAATAGAGGAATG‑AACGATAAAAAAAACGGCCTCCCGGAGGGATGCCGCAACAAGGGGCGCGTGATTTTATGCAga > 1:20140/1‑75 (MQ=255)
tAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg > 1:1301690/1‑76 (MQ=255)
aagagGAATGAAAGNC‑AAAAAAACCGGCCTCCCGGAGGGAAGCCGCTACAAAGTGCGCGTGGTTTTATCCNGTATg < 2:1718657/75‑1 (MQ=255)
agagGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 2:308348/1‑76 (MQ=255)
agagGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 2:1028276/1‑76 (MQ=255)
agagGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 2:1530312/1‑76 (MQ=255)
ggAATGAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggc < 2:535424/76‑1 (MQ=255)
aaaaaaGGAAAAAAAAACCGGCCCCCGGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt < 1:1033644/73‑1 (MQ=38)
tGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 1:397415/1‑76 (MQ=255)
tGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 1:528743/1‑76 (MQ=255)
aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 1:611040/1‑76 (MQ=255)
gaaaaaaaaaCGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACatat < 2:430026/75‑1 (MQ=255)
|
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATAT > REL606/2131383‑2131532
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
