Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
17 |
37.0 |
2377229 |
96.2% |
2286894 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,131,466 |
(A)9→8 |
intergenic (‑125/+175) |
gatY ← / ← fbaB |
D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,131,458 | 0 | A | . | 95.2%
| 84.7
/ 0.2
| 22 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (10/10); minor base T (1/0); total (12/10) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGC > REL606/2131385‑2131522
|
atgaTTTGTTTTTAAATGTCGAGATAACGATGACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 1:469763/1‑76 (MQ=255)
atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa < 2:706144/76‑1 (MQ=255)
tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg > 1:24480/1‑73 (MQ=255)
tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCGAAAAAAAACGGCCt > 2:746957/1‑76 (MQ=255)
tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc < 1:1078507/76‑1 (MQ=255)
tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc > 1:482298/1‑76 (MQ=255)
tAAATGTCGAGAGAACGATCACAAAAACTGCAATATGAAAATTAATAGAGGAATGAATGGC‑AAAAAAAACGGCCTc < 2:1074773/76‑1 (MQ=255)
aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:414725/1‑76 (MQ=255)
aaTGTCGAGATAACGATCGCAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 1:459874/1‑76 (MQ=255)
aaTGTCGAGATAACGATCACAAAACCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 2:220600/1‑76 (MQ=255)
gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa > 2:403460/1‑76 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGANGAATGATAGGC‑AAAAAAAACGGCCTCCCGGAgg > 1:958208/1‑76 (MQ=255)
naTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGaa < 1:1042729/75‑1 (MQ=255)
gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGaa < 2:1114968/76‑1 (MQ=255)
gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGaa < 2:415522/76‑1 (MQ=255)
aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCg > 2:22614/1‑76 (MQ=255)
aTCACAAAAACGACAATATGCAAATCAATAGAGGATTGAAAGG‑TAACAAAAACGTCCTCCCGGAGGGAGGCCGcca > 1:129039/1‑74 (MQ=255)
aTCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaa < 2:815047/76‑1 (MQ=255)
aaaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAg > 1:106944/1‑76 (MQ=255)
aaaaCGACAATATGAAAATAAATAGAGAAAGAAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGt < 2:638147/76‑1 (MQ=255)
aCAATATAAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCTGAGGGAAGCCGCAACAAAGTGCGCGt < 1:1055441/76‑1 (MQ=255)
aaaTATAAAAATAAATAGNGAAATGAAAGG‑AAATAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTg < 1:937601/75‑1 (MQ=255)
aaaaaTTAATAGAGGAATAAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa < 2:449702/75‑1 (MQ=255)
ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg < 2:1106534/76‑1 (MQ=255)
tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 1:342449/1‑76 (MQ=255)
aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt > 2:362181/1‑76 (MQ=255)
aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt < 2:32733/76‑1 (MQ=255)
aTAGAGGAATAAAAGG‑AAAAAAAACCGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt < 2:1159098/76‑1 (MQ=255)
ggAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggc < 2:1134432/76‑1 (MQ=255)
|
ATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGC > REL606/2131385‑2131522
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
