Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A124 F1 I1 R1
|
18 |
228.8 |
14151462 |
95.4% |
13500494 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,131,466 |
(A)9→8 |
intergenic (‑125/+175) |
gatY ← / ← fbaB |
D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,131,458 | 0 | A | . | 96.9%
| 460.3
/ 8.3
| 96 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base . (74/19); total (76/20) |
| Fisher's exact test for biased strand distribution p-value = 5.08e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 |
Only 100 of 164 total aligned reads displayed.
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG > REL606/2131383‑2131533
|
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 2:6976070/1‑76 (MQ=255)
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 1:6787798/1‑76 (MQ=255)
aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 2:2030107/76‑1 (MQ=255)
atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 1:1141939/1‑76 (MQ=255)
atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa < 1:1157195/76‑1 (MQ=255)
atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 2:3317273/1‑76 (MQ=255)
atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa < 1:4385161/76‑1 (MQ=255)
atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 1:5751335/1‑76 (MQ=255)
atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa < 2:4285148/76‑1 (MQ=255)
tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa > 2:6112257/1‑76 (MQ=255)
tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa < 2:1092528/76‑1 (MQ=255)
tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa > 2:5216074/1‑76 (MQ=255)
tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa > 2:5295018/1‑76 (MQ=255)
tgaTGTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa < 1:4851028/76‑1 (MQ=255)
gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa < 2:883753/76‑1 (MQ=255)
gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa < 1:1101173/76‑1 (MQ=255)
aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa < 1:1134604/76‑1 (MQ=255)
aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa < 1:4697142/76‑1 (MQ=255)
aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 2:220319/1‑76 (MQ=255)
aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa < 1:1877397/76‑1 (MQ=255)
ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa < 1:2925093/76‑1 (MQ=255)
ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 2:6901992/1‑76 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 2:1286577/1‑75 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac < 1:4003487/76‑2 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:4559389/1‑75 (MQ=255)
gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg > 1:6063720/1‑74 (MQ=255)
tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg < 2:3607867/76‑4 (MQ=255)
tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg < 2:92561/76‑4 (MQ=255)
tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg > 1:2429409/1‑73 (MQ=255)
tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaaagg > 2:7007304/1‑74 (MQ=255)
tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 2:3693565/1‑76 (MQ=255)
tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 1:6313548/1‑76 (MQ=255)
ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:4261592/1‑76 (MQ=255)
ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:6904131/1‑76 (MQ=255)
tAAATGTCGAGATAACGATCACAAAAACTACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc < 2:1602833/76‑1 (MQ=255)
aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:1394965/1‑76 (MQ=255)
aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑‑AAAAAAACGGCCTccc > 1:2774384/1‑76 (MQ=255)
aaTGTCGAGATAACGATCACAACAACGACAATATGAAAATAAATAGAGGAATGAAAGGC‑AAAATAAACGGCCTccc > 2:5706496/1‑76 (MQ=255)
tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑gg < 2:3337015/76‑1 (MQ=255)
gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGa > 1:5800287/1‑76 (MQ=255)
gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGa > 1:2919038/1‑76 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATTAAAAGTAATAGAGGAATGAAATGC‑AAAAAAAACGGCCTCCC‑GGaat < 2:6625749/76‑3 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 1:4303328/1‑76 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 2:5381666/1‑76 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 2:6294818/1‑76 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGa < 2:5598392/76‑1 (MQ=255)
gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGaa < 1:2697642/76‑1 (MQ=255)
gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGaa < 1:3246484/76‑1 (MQ=255)
tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGc < 1:2091397/76‑1 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGcc < 1:1482421/76‑1 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGcc > 2:1046499/1‑76 (MQ=255)
aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCg > 1:5644379/1‑76 (MQ=255)
aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCg < 1:4896633/76‑1 (MQ=255)
cGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGc > 1:6727708/1‑76 (MQ=255)
cGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGc > 1:7070589/1‑76 (MQ=255)
gATCACAAAAACGACAATAGGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGca < 1:6625256/76‑1 (MQ=255)
cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGcaaca > 2:1924223/1‑76 (MQ=255)
aaaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAg > 1:37805/1‑76 (MQ=255)
cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTgcgc > 2:1762320/1‑76 (MQ=255)
cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTgcgc > 1:3523303/1‑76 (MQ=255)
gACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGcgcg > 2:4701675/1‑76 (MQ=255)
aCAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGt > 2:5697836/1‑76 (MQ=255)
atGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGttt > 2:5127939/1‑76 (MQ=255)
atGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGttt > 1:5624516/1‑76 (MQ=255)
gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa > 1:6211296/1‑76 (MQ=255)
gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa > 1:5523169/1‑76 (MQ=255)
aaaaaTTAATAGAGAAAGGAGATG‑AAAAAAGACCGGCCGCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa < 1:5492563/75‑1 (MQ=255)
aaaaTAAAAGAGGAAATAAAGGGAAAAAAAACCGCCCCCCC‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 2:2984640/76‑1 (MQ=255)
aTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa > 1:2955812/1‑76 (MQ=255)
ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 2:1653160/1‑76 (MQ=255)
tAATAGAGGAATGAAAGGCAAAAAAAAA‑GGCCTCCC‑GGGGGGAAGCCGGCACAAAGGGCGCGTGGGTTTATGCAgg > 2:443703/1‑76 (MQ=255)
tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:5981022/1‑76 (MQ=255)
tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:3192618/1‑76 (MQ=255)
tAATAGAGGAATGAAAGG‑AAAAAAAACCGGCCCCCC‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg < 2:2762632/76‑1 (MQ=255)
aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa > 1:4118083/1‑76 (MQ=255)
agggagGAAAGAAA‑GAAAAAAAAAACGCCCTCCC‑GGAGGTAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt < 2:248305/73‑1 (MQ=255)
aTAGATGAAT‑AAAAGAAAAAAAAAACGGCCCCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt < 1:4154897/76‑1 (MQ=255)
aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt > 1:2023957/1‑76 (MQ=255)
tAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg > 1:6019716/1‑76 (MQ=255)
agagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 2:5584051/1‑76 (MQ=255)
agGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg > 2:3451244/1‑76 (MQ=255)
agGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg > 2:4082163/1‑76 (MQ=255)
ggAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggc > 1:1383984/1‑76 (MQ=255)
aaagAAAGG‑AAAAAAAAACGGCCCCCG‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt < 1:842026/73‑1 (MQ=38)
aaTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt > 1:4937647/1‑76 (MQ=255)
aaTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt > 2:3104908/1‑76 (MQ=255)
aTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa > 1:6988122/1‑76 (MQ=255)
ttaaaGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAACCGCAACCAAGTGCGCGGGGGTTTATGCAGGATGCGGCGTaa > 1:3216147/3‑76 (MQ=255)
tGAAAGGC‑AAAAAAACCGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa < 1:4873019/76‑1 (MQ=38)
tGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 2:2128139/1‑76 (MQ=255)
tGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 2:4730778/1‑76 (MQ=255)
gaaGGCAAAAAAAAACGGCCCACC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaaa < 1:504651/75‑1 (MQ=255)
aaaGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:2241975/1‑76 (MQ=255)
aaaGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:2946137/1‑76 (MQ=255)
aaaGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:2452946/1‑76 (MQ=255)
aaGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACa > 2:1996119/1‑76 (MQ=255)
aGGGAAAAAAAACCGGCCCCCC‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACa < 1:3562744/76‑1 (MQ=37)
agtaaaaaaaacCGGCCCCCC‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACat < 1:4651533/73‑1 (MQ=37)
aaaaaaaacCGGCCCCCC‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATAtg < 1:6441705/76‑1 (MQ=38)
aaaaaaaaaCCGCCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACatat < 2:3196489/76‑1 (MQ=37)
|
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG > REL606/2131383‑2131533
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
