Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
15 |
35.6 |
2322208 |
94.1% |
2185197 |
75.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,131,466 |
(A)9→8 |
intergenic (‑125/+175) |
gatY ← / ← fbaB |
D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,131,458 | 0 | A | . | 91.3%
| 87.8
/ 2.9
| 23 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (15/6); total (16/7) |
| Fisher's exact test for biased strand distribution p-value = 5.26e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACA > REL606/2131383‑2131529
|
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa < 2:252085/76‑1 (MQ=255)
gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 1:345373/1‑76 (MQ=255)
aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 2:910146/1‑76 (MQ=255)
aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 1:452014/1‑76 (MQ=255)
tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 2:452014/76‑1 (MQ=255)
ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 2:283135/1‑76 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 2:110201/1‑75 (MQ=255)
tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg > 1:1126454/1‑73 (MQ=255)
ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGcc > 1:85126/1‑76 (MQ=255)
tAAATGTCGAGAGAACGATCACAAAAACGACAATATGAAAATTAATAGAGGNATGAAAGGC‑AAAAAAAACGGCCTc < 2:207061/76‑1 (MQ=255)
aaTGTCGAGAAAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 1:162301/1‑76 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 1:735973/1‑76 (MQ=255)
cGAGATAACGATCACAAAAACGAAAATATGAAAATTAATAGAGGAATGAAAGGC‑ATAAAAAACGGCCTCCCGGAgg > 1:865193/1‑76 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 1:822131/1‑76 (MQ=255)
tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGc > 1:485472/1‑76 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑GAAAAAAACGGCCTCCCGGAGGGAAGcc > 1:682013/1‑76 (MQ=255)
cGATCACAAAANCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGc > 1:175789/1‑76 (MQ=255)
cacaAAAACAACAAAATAAAAATAAATAGAGAAATAAAAGGC‑AAAAAAAACGGCCCCCCGGAGGGAAGCCGcaaca < 2:827317/76‑1 (MQ=255)
caAAAACGACAATATGAAAATTAATAGAGGAATGAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACaaa < 2:735973/76‑1 (MQ=255)
cAATATGAAAATTAAGTGAGGAATGAAAGGC‑AAGTAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTg < 2:772095/76‑1 (MQ=255)
tGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtttt < 2:442361/76‑1 (MQ=255)
tGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtttt > 1:245131/1‑76 (MQ=255)
tGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtttt > 2:690572/1‑76 (MQ=255)
aaaaaTAAAAAGA‑GAAAGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 2:916121/75‑1 (MQ=255)
aaaaTTAATAGAGGAATGAAGTGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 2:969732/76‑1 (MQ=255)
ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGCTTTTATGCAg < 1:162773/76‑1 (MQ=255)
ttAAAAGAGAAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg < 2:162301/76‑1 (MQ=255)
tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:231604/1‑76 (MQ=255)
tAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGACGCAACAAAGTGCGCGTGGTTTTATGCAGGATg > 1:921341/1‑76 (MQ=255)
gacgAATGAAAGGC‑AAAAAAAACGGCNTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcg > 2:998004/4‑76 (MQ=255)
agGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAGGTGCGCGTGGTTTTATGCAGGATgcgg > 1:483893/1‑76 (MQ=255)
agGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg < 2:715015/76‑1 (MQ=255)
aaTGAAAGG‑AAAAACAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt < 2:721583/76‑1 (MQ=39)
gAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaaa < 2:17599/76‑1 (MQ=255)
aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 1:10692/1‑76 (MQ=255)
aaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACa > 2:164128/1‑76 (MQ=255)
|
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACA > REL606/2131383‑2131529
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
