Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A20 F1 I1 R1
|
17 |
44.7 |
2849266 |
96.8% |
2758089 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,131,466 |
(A)9→8 |
intergenic (‑125/+175) |
gatY ← / ← fbaB |
D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,131,458 | 0 | A | . | 83.3%
| 93.5
/ 16.4
| 30 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (1/4); new base . (21/4); total (22/8) |
| Fisher's exact test for biased strand distribution p-value = 1.12e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATA > REL606/2131385‑2131531
|
atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa < 1:475666/76‑1 (MQ=255)
atgaTTTGTTTTGAAATGTCGANANAATGCTCACANAAACGACAATATGANAATTAATAGTGGAATAGAAGGCaat < 2:24603/76‑2 (MQ=255)
aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 2:163041/1‑76 (MQ=255)
tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 1:616681/76‑1 (MQ=255)
ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 2:251314/1‑76 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:959669/1‑75 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:817261/1‑75 (MQ=255)
tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 1:489260/1‑76 (MQ=255)
ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:674456/1‑76 (MQ=255)
aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:652281/1‑76 (MQ=255)
aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 1:394724/1‑76 (MQ=255)
tGTCGAGATAACGATCACAAAAACGACAATATGNAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCgg > 2:1212035/1‑76 (MQ=255)
gTCGAGATAACGATCACAAAAAAGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa > 1:958044/1‑76 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 2:249484/1‑76 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 1:861173/1‑76 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCAGAGGGa > 1:1398904/1‑76 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc > 2:1244377/1‑76 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc > 1:1259888/1‑76 (MQ=255)
gATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGca > 2:1268172/1‑76 (MQ=255)
gATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGca < 2:222634/76‑1 (MQ=255)
tCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCCCCCGGAGGGAAGCCGcaac < 2:127629/76‑1 (MQ=255)
cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaaca > 2:297808/1‑76 (MQ=255)
aaaaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACaaa < 2:110876/75‑1 (MQ=255)
aaaaCAACAATATGAAAATAAAAAGAGAAATGAAAGG‑AAAAAAAACCGGCCTCGCGGAGGGAAGCCGCAACAAAGt < 2:1123564/76‑1 (MQ=255)
aaCGACAATATGAAAATTAATAGAGGAATGAANGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAANTgc > 2:1323704/1‑76 (MQ=255)
aaCGAAAATATAAAAATTAATAGAGGAATAAAAGGAAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTg < 2:862597/76‑1 (MQ=255)
atGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGttt > 1:1051876/1‑76 (MQ=255)
taanaTTAGTAGAGGAATGAAAGG‑AAAAAAAAACGGCCTCCCGNAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa < 2:1333534/75‑1 (MQ=255)
aaaaaTAAAAAGAGGAATAAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa < 2:659119/75‑1 (MQ=255)
aaaaaGTAGTAAAGGAATAAAAAG‑AAAAAAACGCGGCCTCCCGGGGGAAAGCAGCAACAAAGTGCGCGTGGTTTTa < 2:659131/75‑1 (MQ=255)
aaaaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 2:4510/76‑1 (MQ=255)
cagttAAAAGAGANATAAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATg < 1:183104/73‑1 (MQ=255)
aaaTAAAAAGAGAAAAAAAAGAAAAAAAAAACCGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 1:819562/76‑1 (MQ=255)
ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 1:716690/1‑76 (MQ=255)
tAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 2:458602/1‑76 (MQ=255)
aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa > 1:385959/1‑76 (MQ=255)
aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt > 1:804629/1‑76 (MQ=255)
agGAATGAAAGG‑NAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg > 1:13044/1‑76 (MQ=255)
gAAGGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg < 2:716690/76‑1 (MQ=39)
aTGAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa < 2:291765/76‑1 (MQ=255)
tGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 1:561079/1‑76 (MQ=255)
aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGCGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 1:882670/1‑76 (MQ=38)
aaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACa > 2:591037/1‑76 (MQ=255)
aaaaaaaaaacCGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACata < 1:1092620/74‑1 (MQ=38)
|
ATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATA > REL606/2131385‑2131531
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
