Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A122 F1 I1 R1
|
27 |
151.2 |
10056804 |
92.4% |
9292486 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,131,466 |
(A)9→8 |
intergenic (‑125/+175) |
gatY ← / ← fbaB |
D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,131,458 | 0 | A | . | 97.1%
| 323.3
/ 1.1
| 68 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (54/12); total (55/13) |
| Fisher's exact test for biased strand distribution p-value = 3.48e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 127 total aligned reads displayed.
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATA > REL606/2131383‑2131531
|
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 2:1627661/1‑76 (MQ=255)
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 2:1809963/1‑76 (MQ=255)
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa < 2:2443575/76‑1 (MQ=255)
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 2:3582257/1‑76 (MQ=255)
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa < 2:1265098/76‑1 (MQ=255)
aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:2914009/76‑1 (MQ=255)
aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:4147555/76‑1 (MQ=255)
aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa > 1:3953927/1‑76 (MQ=255)
aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa > 2:2334256/1‑76 (MQ=255)
aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:4713476/76‑1 (MQ=255)
aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:1027066/76‑1 (MQ=255)
atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 1:8888/1‑76 (MQ=255)
tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa < 1:3388452/76‑1 (MQ=255)
gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 1:2191201/1‑76 (MQ=255)
aTTTGTTTTTAAATGTCGAGATAACGATCACAACAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 1:4863977/1‑76 (MQ=255)
aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 2:4265386/1‑76 (MQ=255)
aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 2:490355/1‑76 (MQ=255)
tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTTATAGAGGAATGAAAGGCaaaaaaa > 2:372972/1‑76 (MQ=255)
tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 1:1205616/76‑1 (MQ=255)
tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 2:623802/76‑1 (MQ=255)
tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa > 1:3321190/1‑76 (MQ=255)
ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 1:1496173/1‑76 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 2:531527/1‑75 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:887820/1‑75 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac < 2:458178/76‑2 (MQ=255)
gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg < 1:2175816/76‑3 (MQ=255)
tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg > 1:3899085/1‑73 (MQ=255)
tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 2:4883057/1‑76 (MQ=255)
tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 2:1467670/1‑76 (MQ=255)
tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 1:249554/1‑76 (MQ=255)
aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:1328659/1‑76 (MQ=255)
aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:828588/1‑76 (MQ=255)
aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:4017385/1‑76 (MQ=255)
aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAAGC‑AAAAAAAACGGCCTcc < 2:3284951/76‑1 (MQ=255)
aTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCg > 2:1591635/1‑76 (MQ=255)
aTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCg < 2:3940249/76‑1 (MQ=255)
tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCgg > 2:1800447/1‑76 (MQ=255)
tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCgg > 2:4131386/1‑76 (MQ=255)
gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa < 2:1237466/76‑1 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg < 2:1445799/76‑1 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 1:1473820/1‑76 (MQ=255)
gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAggg < 1:3290980/76‑1 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 2:1323605/1‑76 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 2:3712411/1‑76 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa < 2:65226/76‑1 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 1:1517576/1‑76 (MQ=255)
agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 1:4097306/1‑76 (MQ=255)
gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGaa < 1:3712609/76‑1 (MQ=255)
aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAg < 2:2202510/76‑1 (MQ=255)
aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAg < 2:1615367/76‑1 (MQ=255)
tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGc > 2:2525368/1‑76 (MQ=255)
tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGc > 1:3661061/1‑76 (MQ=255)
tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGc > 1:2688584/1‑76 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc < 2:1884616/76‑1 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc > 2:4100899/1‑76 (MQ=255)
cGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGc > 2:4264962/1‑76 (MQ=255)
aTCAAAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGG‑AAAAAAAAACGGCCCCCCGGAGGGAAGCCGcaa < 2:3517488/76‑1 (MQ=255)
tCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaac > 2:3944440/1‑76 (MQ=255)
acaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaacaa > 1:1241216/1‑76 (MQ=255)
acaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaacaa > 1:2041374/1‑76 (MQ=255)
caAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACaaa > 1:4366483/1‑76 (MQ=255)
aaaCGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGt > 2:2628966/1‑76 (MQ=255)
aaaCAAAAATATAAAAATTAAAAGAGAAAAAAAAGG‑AAAAAAAACCGCCCCCCGGGGGGAAAGCCGCAACAAAGTg < 2:2673377/76‑1 (MQ=255)
aaaCAAAAATATAAAAATAAAAAGGGGAATAAAAGG‑AAAAAAAACCGCCCCCCCGGGGGGAAGCCGCAACAAAGTg < 2:440406/76‑1 (MQ=255)
aCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgcg > 2:3372458/1‑76 (MQ=255)
cGACAATATGAAAATTAATAGAGGATTGACTGGCAAAAAAACACGGCCTCCCGGAGGGCAGCCGCAAAAACGTgcg > 2:4810168/1‑76 (MQ=255)
aCAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGt > 2:176351/1‑76 (MQ=255)
aCAATATGAAAATTAATAGAGGAATGAAAGG‑AAAAAAAAACGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGt < 1:4891443/76‑1 (MQ=255)
aaTATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTgg > 2:1348982/1‑76 (MQ=255)
aaTATAAAAATTAATAGAGGAAT‑AAAAGCAAAAAAAACCGGCCCCCCGGGGGAAAGCCGCAACAAAGTGCGCGTgg < 2:3395446/76‑1 (MQ=255)
aaTATAAAAATAAATAGAGAAATAAAAGG‑AAAAAAAACCGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTgg < 1:4076377/76‑1 (MQ=255)
atatGAAAATAAATAGAGAAATGAAATAAAAAAAAAACCGCCCTCCCGGAGGAAAGCCGCAACAAAGTGCGCGTgg < 1:516569/76‑1 (MQ=255)
tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtt > 1:747598/1‑76 (MQ=255)
ataaaaaTTAATAGAGAAAT‑AAAAGAAAAAAAAACCGCCCCCCCGGAGGAAAGCCGCAACAAAGTGCGCGTGGttt < 2:3044559/73‑1 (MQ=255)
aaaaaaaTAAAGAGA‑GAAAGAAAAGAAAAAAAAACCGCCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGttt < 2:1371705/73‑1 (MQ=255)
tGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtttt > 2:2227232/1‑76 (MQ=255)
aaaaaaTTAAAGGAGAAAT‑AAAAGAAAAAAAAACCGGCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGtttt < 2:4772010/74‑1 (MQ=255)
gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa > 1:3833763/1‑76 (MQ=255)
gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa > 1:4452969/1‑76 (MQ=255)
gAAAATAAATAGAGGAATAAAAGGC‑AAAAAAACCGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa < 1:3281980/76‑1 (MQ=255)
aaaaTAAATAG‑GGAAGTAAAGGAAAAAAAAACCGCCCCCCCGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 2:2160868/76‑1 (MQ=255)
aaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGc > 2:2032575/1‑76 (MQ=255)
aaTTAAAAGAGGAATGAAA‑GAAAAAAAAACCGGCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGc < 2:2094843/76‑1 (MQ=255)
ataaaTAGAGAAATGAAAGG‑AAAAAAACCCGGCCTCCCGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa < 1:2518215/73‑1 (MQ=255)
aTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa > 1:3099766/1‑76 (MQ=255)
aTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa > 1:2840996/1‑76 (MQ=255)
tAATAGAGGAATGAAAGGC‑AAAAAAACCGCCCCCCCGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg < 1:4265386/76‑1 (MQ=255)
tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:2874196/1‑76 (MQ=255)
tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:1570763/1‑76 (MQ=255)
tAAAAGAGGAATAAAAGG‑AAAAAAAACCGCCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg < 1:2791616/76‑1 (MQ=255)
aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa > 2:1105382/1‑76 (MQ=255)
agagGAATGAAA‑GAAAAAAAAACCGCCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc < 1:3283949/76‑1 (MQ=255)
gAATGAAA‑GAAAAAAAAACCGGCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg < 2:145391/76‑1 (MQ=37)
aTGAAAGAC‑AAAAAAACCGGCCTCCCGGGGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa < 2:1058235/76‑1 (MQ=37)
tGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 1:1160307/1‑76 (MQ=255)
aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:4095960/1‑76 (MQ=255)
aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 1:3308218/1‑76 (MQ=255)
aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:2030404/1‑76 (MQ=255)
aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGGGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:1036054/1‑76 (MQ=38)
ggaaaaaaaaaCGCCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACata < 1:2239134/74‑1 (MQ=37)
|
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATA > REL606/2131383‑2131531
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
