Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A132 F1 I1 R1	21	265.3	16744490	95.4%	15974243	76.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,131,466	(A)_9→8	intergenic (‑125/+175)	gatY ← / ← fbaB	D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,131,458	0	A	.	99.0%	481.9 / ‑1.5	96	intergenic (‑117/+183)	gatY/fbaB	D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (76/19); total (77/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 145 total aligned reads displayed.

AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG  >  REL606/2131383‑2131533

aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCa                                                                               <  1:3689277/76‑1 (MQ=255)

  atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaa                                                                             <  1:2622638/76‑1 (MQ=255)

  atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaa                                                                             >  1:7059994/1‑76 (MQ=255)

  atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaa                                                                             >  1:8305959/1‑76 (MQ=255)

  atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaa                                                                             <  1:4035631/76‑1 (MQ=255)

   tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaa                                                                            <  1:3746342/76‑1 (MQ=255)

    gaTTTGTTTTTAAATGTCGAGATAACGATCACAGAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaa                                                                           >  1:7863706/1‑76 (MQ=255)

    gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaa                                                                           <  1:5348447/76‑1 (MQ=255)

    gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaa                                                                           <  2:7964621/76‑1 (MQ=255)

     aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaa                                                                          <  1:3987330/76‑1 (MQ=255)

      tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaa                                                                         >  1:3149259/1‑76 (MQ=255)

      tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaa                                                                         <  1:5211860/76‑1 (MQ=255)

        tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaaac                                                                       >  2:5764755/1‑75 (MQ=255)

        tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaaac                                                                       >  2:2621159/1‑75 (MQ=255)

        tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaaac                                                                       <  1:7049791/76‑2 (MQ=255)

        tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaaac                                                                       <  2:1465668/76‑2 (MQ=255)

         gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGACaaaaaaaaag                                                                      >  2:633806/1‑75 (MQ=255)

          tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCaaaaaaaacgg                                                                     <  1:5591711/76‑4 (MQ=255)

            tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGcc                                                                  >  1:756465/1‑76 (MQ=255)

             ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt                                                                 >  2:2322721/1‑76 (MQ=255)

             ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt                                                                 >  2:5451966/1‑76 (MQ=255)

             ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt                                                                 <  2:7240421/76‑1 (MQ=255)

              tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc                                                                >  2:1115042/1‑76 (MQ=255)

              tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc                                                                >  1:112085/1‑76 (MQ=255)

              tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc                                                                >  1:1475652/1‑76 (MQ=255)

               aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc                                                               >  2:140302/1‑76 (MQ=255)

               aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc                                                               >  1:1182578/1‑76 (MQ=255)

               aaaTGTCGAGATAACAATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc                                                               <  1:4854686/76‑1 (MQ=255)

                aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc                                                              >  2:2268715/1‑76 (MQ=255)

                  tGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑gg                                                           <  1:363159/76‑1 (MQ=255)

                   gTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGa                                                          >  1:2210821/1‑76 (MQ=255)

                    tCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAg                                                         >  1:2238312/1‑76 (MQ=255)

                    tCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAg                                                         <  1:4772940/76‑1 (MQ=255)

                     cGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg                                                        >  1:843993/1‑76 (MQ=255)

                     cGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg                                                        >  2:6637091/1‑76 (MQ=255)

                     cGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg                                                        >  2:5627239/1‑76 (MQ=255)

                     cGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg                                                        >  1:3060452/1‑76 (MQ=255)

                     cGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg                                                        >  1:1990843/1‑76 (MQ=255)

                     cGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑‑AAAAAAACGGCCTCCC‑GGAggg                                                       >  1:3284837/1‑76 (MQ=255)

                      gagaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑‑AAAAAAACGGCCTCCC‑GGAGGG‑a                                                     >  2:3926284/1‑76 (MQ=255)

                       agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a                                                     >  2:8247817/1‑76 (MQ=255)

                       agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a                                                     <  2:686758/76‑1 (MQ=255)

                       agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a                                                     >  1:1247298/1‑76 (MQ=255)

                       agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a                                                     <  1:406391/76‑1 (MQ=255)

                       agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a                                                     <  2:3013820/76‑1 (MQ=255)

                       agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a                                                     >  1:8146209/1‑76 (MQ=255)

                       agaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a                                                     <  2:2847852/76‑1 (MQ=255)

                        gaTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑aa                                                    >  2:5194685/1‑76 (MQ=255)

                         aTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAg                                                   <  1:2443963/76‑1 (MQ=255)

                         aTAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAg                                                   >  2:1914898/1‑76 (MQ=255)

                          tAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGc                                                  <  1:4596924/76‑1 (MQ=255)

                          tAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGc                                                  >  2:589459/1‑76 (MQ=255)

                          tAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGc                                                  >  1:498055/1‑76 (MQ=255)

                           aaCGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGcc                                                 >  2:3128894/1‑76 (MQ=255)

                           aaCGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGcc                                                 >  1:285258/1‑76 (MQ=255)

                            aCGATCACAAAAACGACAATATGAAAATT‑AATAGAGGATTGAAAGGC‑AAAAAAAACGGCCCCCC‑GGAGGG‑AAGCCg                                                <  2:8359786/76‑1 (MQ=255)

                             cGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGc                                               >  2:7789015/1‑76 (MQ=255)

                              gATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGca                                              >  2:3406753/1‑76 (MQ=255)

                                tCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGcaac                                            >  2:5052256/1‑76 (MQ=255)

                                 cacaAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑‑AAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGcaacaa                                          >  1:7573990/1‑76 (MQ=255)

                                  acaAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGcaacaa                                          >  1:1097176/1‑76 (MQ=255)

                                  aaaaaaaCGAAAATAGAAAAATA‑AATAGAGGAAT‑AAAGG‑AAAAAAAAACCGCCCCCC‑GGGGGGAAAGCCGcaacaa                                          <  2:5193815/74‑1 (MQ=255)

                                      aaaCGAAAATAGAAAAATA‑AATAGAGAAAT‑AAAAGAAAAAAAAAACGCCCCCCC‑GGAGGA‑AAGCCGCAACAAAGTg                                      <  2:7201555/76‑1 (MQ=255)

                                       aaCGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTgc                                     >  2:6393602/1‑76 (MQ=255)

                                        aCGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTgcg                                    >  1:6424646/1‑76 (MQ=255)

                                         cGACAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTgcgc                                   >  2:3799996/1‑76 (MQ=255)

                                           aCAATATGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGt                                 >  1:7761178/1‑76 (MQ=255)

                                                atGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGttt                            >  2:4786514/1‑76 (MQ=255)

                                                atGAAAATT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGttt                            >  2:4698240/1‑76 (MQ=255)

                                                 agAAAATT‑AA‑AAAAGAAAAAAAAGCAAAAAAAACCGGCCTCCC‑GGGGAA‑AAGCCGCAACAAAGTGCGCGTGGtttt                           <  2:1262410/75‑1 (MQ=255)

                                                  aaaaaTTAAATAGAGAAAT‑AAAAGAAAAAAAAACCGCCCCCCG‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTGGtttt                           <  2:6978467/75‑1 (MQ=255)

                                                   aaaaTA‑AATAGAGAAAAG‑AAGGGAAAAAAAAACGCCCCCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTAt                         <  1:8010048/76‑1 (MQ=255)

                                                    aaaTT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATg                        >  2:7994111/1‑76 (MQ=255)

                                                     aata‑aataGAGGAAAAAAAGG‑AAAAAAAAACGGCCCCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGc                       <  1:2566059/76‑1 (MQ=255)

                                                     aaTT‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGAGGTTTTATGc                       >  1:3561359/1‑76 (MQ=255)

                                                     aaTT‑AATAGAGAAATGAAAGGC‑AAAAAAACCGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGc                       <  2:4118606/76‑1 (MQ=255)

                                                     aaTT‑AATAGAGAAATAAAAGG‑AAAAAAAACCGGCCCCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGc                       <  1:860849/76‑1 (MQ=255)

                                                       tt‑AATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg                     >  1:1512133/1‑76 (MQ=255)

                                                          aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa                   >  2:318859/1‑76 (MQ=255)

                                                          aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa                   >  2:318858/1‑76 (MQ=255)

                                                          aaTAGAGGAATGAAAGG‑AAAAAAAACCGGCCCCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa                   <  1:6118210/76‑1 (MQ=255)

                                                           aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt                  >  1:730083/1‑76 (MQ=255)

                                                           aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt                  >  2:7601401/1‑76 (MQ=255)

                                                           aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt                  >  2:6943284/1‑76 (MQ=255)

                                                           aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt                  >  2:2152534/1‑76 (MQ=255)

                                                            tAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg                 >  1:599440/1‑76 (MQ=255)

                                                            aagagGAATAAAAGGC‑AAAAAAAACGGCCCCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg                 <  1:1989364/75‑1 (MQ=255)

                                                             agagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc                >  1:2929859/1‑76 (MQ=255)

                                                             agagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc                >  1:331941/1‑76 (MQ=255)

                                                             agagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc                >  1:5962003/1‑76 (MQ=255)

                                                             agagGAATGAAAGGC‑‑AAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcg               >  2:1204678/1‑76 (MQ=255)

                                                                ggAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg              >  2:4976491/1‑76 (MQ=255)

                                                                ggAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggc             >  1:4603905/1‑76 (MQ=255)

                                                                  aaTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGGTTTATGCAGGATGCGGCGt           >  2:7076138/1‑76 (MQ=39)

                                                                    agaaaagaaaaAAAAACCGCCCCCCG‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa          <  2:3486585/75‑1 (MQ=25)

                                                                      aaaGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc       >  2:2398472/1‑76 (MQ=255)

                                                                       aacgCAAAAAAAAGCGCCCCGCG‑GGGGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc       <  2:5782211/73‑1 (MQ=21)

                                                                            aaaaaaaacCGGCCCCCG‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATAtg  <  1:1223109/76‑1 (MQ=38)

                                                                            aaaaaaaaaCGGCCCCCC‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATAtg  <  2:2112338/76‑1 (MQ=255)

                                                                            aaaaaaaaaCCGCCCCCCGGGAGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACatat   <  1:5545923/76‑1 (MQ=37)

AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATT‑AATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG  >  REL606/2131383‑2131533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A