Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A55 F1 I1 R1
|
18 |
62.6 |
3986840 |
96.5% |
3847300 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,131,466 |
(A)9→8 |
intergenic (‑125/+175) |
gatY ← / ← fbaB |
D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,131,458 | 0 | A | . | 96.8%
| 141.2
/ ‑1.0
| 31 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (27/3); total (28/3) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 |
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACAT > REL606/2131383‑2131530
|
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa < 2:976684/76‑1 (MQ=255)
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa < 2:1977957/76‑1 (MQ=255)
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa < 2:35456/76‑1 (MQ=255)
aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 1:1829938/1‑76 (MQ=255)
aaatgaTTTGTTTTTAAATGTCGAGATAACGAGCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa < 2:35478/76‑1 (MQ=255)
tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa > 1:1766086/1‑76 (MQ=255)
gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 2:1864134/1‑76 (MQ=255)
tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 1:1814005/76‑1 (MQ=255)
tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 1:635268/76‑1 (MQ=255)
tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 1:1517848/76‑1 (MQ=255)
ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 1:1560134/1‑76 (MQ=255)
ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 2:1633776/1‑76 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac < 1:1363365/76‑2 (MQ=255)
tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac < 1:543265/76‑2 (MQ=255)
gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg > 1:1570010/1‑74 (MQ=255)
tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg > 1:1519739/1‑73 (MQ=255)
ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 1:965980/1‑76 (MQ=255)
ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 1:423002/1‑76 (MQ=255)
tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc > 1:1411481/1‑76 (MQ=255)
aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 2:29379/1‑76 (MQ=255)
gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa > 1:533516/1‑76 (MQ=255)
cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 1:1748196/1‑76 (MQ=255)
gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAggg > 1:376096/1‑76 (MQ=255)
aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAg > 2:89286/1‑76 (MQ=255)
aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAg > 2:737642/1‑76 (MQ=255)
aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAg > 2:1266988/1‑76 (MQ=255)
tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGc > 1:1550227/1‑76 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc < 2:325780/76‑1 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc > 1:1231481/1‑76 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc > 1:393786/1‑76 (MQ=255)
aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAAGC‑AAAAAACACGGCCTCCCGGAGGGAAGcc < 2:110065/76‑1 (MQ=255)
cGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGc > 2:1673241/1‑76 (MQ=255)
gATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGc > 2:123187/1‑76 (MQ=255)
aTCAAAAAAACGAAAATATGAAAATTAATAAAGGAATAAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaa < 2:1829938/76‑1 (MQ=255)
acaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaacaa > 2:1118288/1‑76 (MQ=255)
caAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACaaa > 2:1290532/1‑76 (MQ=255)
aaaaaaCGACAATATGAAAATTAATAGAGGAATAAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACaaa < 2:1460840/75‑1 (MQ=255)
aaaaaaCGAAAATATAAAAATAAATAGAGGAAAAAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACaaa < 2:522096/75‑1 (MQ=255)
aaaCGACAATAGAAAAATTAAAAAAGGAAGAAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTg < 2:220929/76‑1 (MQ=255)
cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgcgc > 1:336378/1‑76 (MQ=255)
gACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGcgcg > 2:932550/1‑76 (MQ=255)
gACAATATAAAAATTAATAGAGAAATAAAAGGC‑AAAAAAACCGGCCTCCCGGAGGGAAGCCGCAACAAAGTGcgcg < 1:280091/76‑1 (MQ=255)
aaTATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAGCGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTgg < 2:1837173/76‑1 (MQ=255)
tGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtttt > 1:543080/1‑76 (MQ=255)
gAAAATTAATAGAGGAATAAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa < 2:1307930/76‑1 (MQ=255)
aaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGc > 1:249399/1‑76 (MQ=255)
aaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGc > 1:1479106/1‑76 (MQ=255)
aTAGAGGAATGAAA‑GAAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt < 2:1677946/76‑1 (MQ=255)
tAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg > 1:721136/1‑76 (MQ=255)
tAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTCATGCAGGATg > 1:261886/1‑76 (MQ=255)
agagGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 1:1453285/1‑76 (MQ=255)
agagAAATAAAAGAC‑AAAAAAAGCGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc < 2:752917/76‑1 (MQ=255)
aaTGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt > 1:1086394/1‑76 (MQ=255)
aaTGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt > 2:277230/1‑76 (MQ=255)
tGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 2:189174/1‑76 (MQ=255)
aaaGGGCAAAAAGACCGGCCTCCGGGGGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaaa < 1:638300/76‑1 (MQ=25)
aaagCAAAAAAAAACGGCCGCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc < 1:1961973/73‑1 (MQ=255)
aagaaaaaaacaCGGGCTCCCGGGGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACat < 1:144440/73‑1 (MQ=37)
|
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACAT > REL606/2131383‑2131530
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
