Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F2 I0 R1 193 6708.0 8620653 98.5% 8491343 136.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,640,925 A→C 10.0% R7S (AGA→AGC)  ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,640,9250AC10.0% 211.1 / 3.1 127R7S (AGA→AGC) ygfMmolybdopterin‑dependent oxidoreductase FAD‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (61/50);  new base C (2/14);  total (63/64)
Fisher's exact test for biased strand distribution p-value = 2.30e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TGCGCAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCAGGATCTGGAGC  >  NZ_CP022686/1640797‑1641064
                                                                                                                                |                                                                                                                                           
tGCGCAAGCCAGAAAAGCCGCTGCCATTATGTGGCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGt                                                                                                                                  <  3:652547/140‑1 (MQ=255)
 gcgcAAGCCAGAAAAGCCGCTGCCAGTAGGTGGCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCGTTCATAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCCGACCCGACTCTGTc                                                                                                                                 >  2:402490/1‑140 (MQ=255)
    cAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTTGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAATGCGATGAATGAACAATTTTTCAGACCCGACACTGTCGac                                                                                                                              >  1:95212/1‑139 (MQ=255)
      aGCCAGATACCCCTCTTCCTGTTTGTGGCTGTGGATTGATGCGCTGGAATAAATAACAGATCCCCTGTTATTTCAGAGAAGAGGGGAAATTAAGGAAACACGATGATTTAACAATTTTTCAGACCCGACTCTGTCGAACa                                                                                                                            <  2:1075092/140‑1 (MQ=255)
      aGCCAGAAAAGCCGCTGCCTGTATGTGGCGTCGGTTGGATGCGCTGGAATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGTGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACa                                                                                                                            <  2:388433/140‑1 (MQ=255)
       gCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACaa                                                                                                                           >  1:454151/1‑140 (MQ=255)
           taaaaGCCGCTGCCTTTTTGTGGGGTCGTTTGGATGCGCTGGAATAAATAAAAGCTCCCCTCTTCTTTCAGATAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAgcgc                                                                                                                       <  4:610605/139‑1 (MQ=255)
            aaaaGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGCCTCTGTCGAACAAGCGCt                                                                                                                      >  5:61370/1‑140 (MQ=255)
                 ccGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTTAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAAc                                                                                                                 >  7:492972/1‑140 (MQ=255)
                 ccGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAAc                                                                                                                 >  1:427280/1‑140 (MQ=255)
                 ccGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGACGAACAAGCGCTGGAAc                                                                                                                 >  1:580295/1‑140 (MQ=255)
                 ccGCTGCCAGTATGTGGCGTCGGATGGATCCGCTGGCATAAATAACAGCTCCCCTCTTATATCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATATTTAAGACCCGACTCTGTCGAACCAGAGCAGGAAc                                                                                                                 >  4:236655/1‑140 (MQ=255)
                  cGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACACGCGCTGGAAcg                                                                                                                >  1:121963/1‑139 (MQ=255)
                   gCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGCCGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTg                                                                                                               >  8:206539/1‑140 (MQ=255)
                           ttgggggcggcGGATGTATGCGCTGGCATAAAAAACACCTCCCCTCTTTTTTCAGAGAAGTGGGTAAATTAAGTAAACACGATGATTGAAAATTTTTTCCGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGcgccg                                                                                                       <  2:450345/135‑1 (MQ=255)
                               gggggtgggatggatGCGCGGGCATAAATAACAGCGCCCCTCTTCTTTCAGATAAGAGGGTAAATTAAGGAAACACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTAc                                                                                                   <  2:454151/133‑1 (MQ=255)
                               gggcggcggcTGGATGCGCTGGCATAAATAACCGCTCCCCTATTCTTTCACAGATGAGGGTAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTAc                                                                                                   <  2:1037900/139‑1 (MQ=255)
                               gggCGTCGGTTGGAGGCGCTGGAATAAATAACAGCTCCCAGTTTATTTCAGAGAAGTGGGTAATTTAAGCAAAGACGATGATTCAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTAc                                                                                                   <  2:1120584/139‑1 (MQ=255)
                                 gCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGCCTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCa                                                                                                 >  7:21315/1‑140 (MQ=255)
                                 gCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCa                                                                                                 >  7:500665/1‑140 (MQ=255)
                                    tCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGa                                                                                              >  7:438872/1‑140 (MQ=255)
                                    tCGGATGGATGCGCAGGCATAAATAACAGCTCCCCTCTACTTTCAGAGAAGAGGGGAAATTAAGGAATGACGATGATTGAACAAATTTTCAGACCAGACACAGACGAACAAGCCCTGGAACTGAAGCGCCGATACCAGGa                                                                                              >  3:1077237/1‑140 (MQ=255)
                                     cGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCATAGAAGAGGGGAAATTAAGGAAAGACGATGATTTAACAAATTTTCAGACCCGACTCTGTCGAAAAAGCGATGGAACTTAAGCGACGCTACCAGGAt                                                                                             >  4:467462/1‑140 (MQ=255)
                                     cGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGCCCCGACTCTGTCGAAAAAGCGCTGGAACTGAAGCGCCGCTACCAGGAt                                                                                             >  8:695561/1‑140 (MQ=255)
                                     cGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGAt                                                                                             >  2:624372/1‑140 (MQ=255)
                                     cGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGAt                                                                                             >  2:569010/1‑140 (MQ=255)
                                     cGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGAt                                                                                             >  1:150635/1‑140 (MQ=255)
                                            ttgCGCTGGAATAAATAACCGCTCCCCTATTCTTTCTGTCAAGAGGGTAAATTAAGTAAAGACTATTATTGAACAATTTTTAAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCg                                                                                      <  6:666222/139‑1 (MQ=255)
                                            atgCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGCCTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCg                                                                                      >  7:138588/1‑140 (MQ=255)
                                            atgCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCg                                                                                      >  2:360668/1‑140 (MQ=255)
                                            atgCGCTGGCATAAATAACAGCTCCCCACTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGAATGAACAATTTTTAAGACCCGACTCTGTCGAACCAGCGCTGGAACTGAAGCGCCGCAACCAGGATGAAGCCg                                                                                      >  3:701433/1‑140 (MQ=255)
                                                 cTGGCATAAATAACATCTCCCCACTTATTTCAGAGAAGAGGGGAATTTAAGGAAAGACGATGATTGAACAATTTTTCATACCCGACTCTGTCGCACAAGCGCTGGCCCTGAAGCGCCGCTACCAGGATGAAGCCGGCTgg                                                                                 >  1:1051505/1‑140 (MQ=255)
                                                 cTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTgg                                                                                 >  8:731012/1‑140 (MQ=255)
                                                  tGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGt                                                                                >  5:1030198/1‑140 (MQ=255)
                                                  gggCATAAATACCAGCACCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAACACGATTATTCAACACTTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGt                                                                                <  2:1026131/139‑1 (MQ=255)
                                                       aaaataaCTGGTCCCCGCTTTTTTAAGATACGTGGGTAAATTAATGAATGACGATGATTGACCAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGcc                                                                           <  7:281751/139‑1 (MQ=255)
                                                              cAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAATGACGAGGATTGCACAATTTTTCAGACCCGTCTCTGTCGAACCCGCGCTGGACCTGCAGCGCCGCTGCCAGGTTGAAGCCGGCTGGTACGCCGGGGgcg                                                                    >  7:770869/1‑139 (MQ=255)
                                                              cAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCCGACCCGCCTCTGTCTAACACGCGCTGGCACTGAAGCGCCGCTACCAGGATGAGGCCGTCTGGTTCGCCGGGGgcc                                                                    >  1:1076571/1‑139 (MQ=255)
                                                              cAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGCCCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGATACCAGGATGAAGCCGTCTGGTTCGCGGGGGtca                                                                    >  2:561224/1‑140 (MQ=255)
                                                              cAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgca                                                                    >  1:1063744/1‑140 (MQ=255)
                                                              cAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgca                                                                    >  2:170668/1‑140 (MQ=255)
                                                              cAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTAACAGGATGAAGCCGTCTGGTTCGCCGGGGgca                                                                    >  5:944687/1‑140 (MQ=255)
                                                                gCTCCCATCTTATTTCACATACGAGGGTAAATTAAGTAAAGACGATGATTGAACAATTTTTCACACCCTACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgcagc                                                                  <  3:404327/140‑1 (MQ=255)
                                                                ccgccccTCTTCTTTCATAGACGTGGGTAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgcagc                                                                  <  8:750433/137‑1 (MQ=255)
                                                                   ccccTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgcagcaca                                                               >  6:303644/1‑138 (MQ=255)
                                                                     cgtcttctTTCAGATATTTGGGTAAATTAAGGAAAGACGATGATTTAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACt                                                             <  6:1030198/138‑1 (MQ=255)
                                                                     ccTCTTCTTTCAGAGATGAGGGTAAATTAAGGAAAGACGATGATTTAACATTTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTGCGCCGGGGGCAGCAAACt                                                             <  6:596410/140‑1 (MQ=255)
                                                                     ccTCTTCTTTCAGACAAGAGGGGAAATTAAGGAAAGACGATGATTCAACAATTTTTCCGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGTATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACt                                                             <  4:794648/140‑1 (MQ=255)
                                                                     ccTCTTCATTCCGATAAGTGGGGAAATTAATCAAAGACTATGATTGAAAAATTTTTCAGACCCGACTCTGTTGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACt                                                             <  5:392848/140‑1 (MQ=255)
                                                                     ccTCTTATTTAAGTTATGTGGGTAAATTTAGCAAAGACGTTGATTGAAAAATTTTACAGCCCCGACGCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACt                                                             <  3:334351/140‑1 (MQ=255)
                                                                      ctctTCTTTCAGAGAAGATGGGAAATTAAGCATAGACGATGATTGACCAATTTTTCAGACCCGACTCTGTCGACCAAGCGCAGGAACAAAAGCGCCGCTACCAGGATGAAGCCGACTGGTTCGCCCGGGGCACCAAACTc                                                            >  1:870433/1‑140 (MQ=255)
                                                                      ctctTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCATCTGGTTCGCCGGGGGCAGCAAACTc                                                            >  2:936026/1‑140 (MQ=255)
                                                                          tcttTCATAGAATTGGGGAAATTAAGGAAACACGATGATTCAAAAATTTTTCACACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACg                                                        <  6:467435/140‑1 (MQ=255)
                                                                          tcttTCAGATAAGCGGGTAAATTAAGGAACGACGATTATTGAAAAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTTAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACg                                                        <  6:591316/140‑1 (MQ=255)
                                                                          tcttTCAGACAAGAGGGTAAATTAAGTAAAGACTATGATTTAAAAATTTTTAACACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACg                                                        <  2:869032/140‑1 (MQ=255)
                                                                           cttTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGAGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAActc                                                       >  8:455150/1‑138 (MQ=255)
                                                                              tCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTac                                                    >  1:259468/1‑140 (MQ=255)
                                                                              tCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTATGTCGAACAAGCGCTCGAACTGAAGAGCCGCTACCAGGATGAAGCCTTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTac                                                    >  1:672490/1‑140 (MQ=255)
                                                                               aatataagtGGGTAAATTATGTAAAGACTTTTATTTAAAAATTTTTCCGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTaca                                                   <  3:59861/135‑1 (MQ=255)
                                                                               aagctaagaGGGTAAATTAAGCAAAGACGATTATTTAAAAATTTTTCATACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTaca                                                   <  4:1008844/135‑1 (MQ=255)
                                                                                agagAAGTGGGTAAATTAAGGAAAGACGATTATTGAACAATTTTTCAGCCCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTacac                                                  <  3:916888/140‑1 (MQ=255)
                                                                                 gagaAGTGGTGAAATTAAGGAAAGACGATGATTGAAAATTTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACAcc                                                 <  3:692632/140‑1 (MQ=255)
                                                                                   gaagaGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCaa                                               >  1:961201/1‑140 (MQ=255)
                                                                                         gttaaaTTACGCAAAGACTATTATTCAAATATTTTTCCGCCCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTa                                         <  5:678587/137‑1 (MQ=255)
                                                                                         gggAAATTAAGGAAAGACTATTATTTAACAATTTTTCAGACCCGACTCTTTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTa                                         <  2:498780/140‑1 (MQ=255)
                                                                                         gggAAATTAAGGAAAGACGATGATTCAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTa                                         <  4:905500/140‑1 (MQ=255)
                                                                                         cggAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGATCAAGCGCTGGAACTGAAGCGCAGCTACCAGGATGAAGCCGTCTGGTTCGCCGCGGGCAGCAAACTCAACGCACCACCAACCCgaa                                         >  8:846456/2‑138 (MQ=255)
                                                                                          ggAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTAc                                        <  8:846016/140‑1 (MQ=255)
                                                                                            aaaTTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCg                                      >  7:615827/1‑140 (MQ=255)
                                                                                               ttAAGGAAAGACGATGATTGAAAAATTTTTCCTCCCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATa                                   <  8:138588/140‑1 (MQ=255)
                                                                                                aaaGTAAAGACGATTATTTAACAATTTTTCAGCCCCGACTCTGCCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGATCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATaa                                  <  3:882596/139‑1 (MQ=255)
                                                                                                   ggAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATaaaaa                               >  4:409180/1‑140 (MQ=255)
                                                                                                     aaaGACTATTATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGa                             <  1:313252/140‑1 (MQ=255)
                                                                                                     aaaGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGa                             >  8:722238/1‑140 (MQ=255)
                                                                                                       aGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGAtt                           >  5:792502/1‑140 (MQ=255)
                                                                                                         aCGATGATTGAACAATTTTTCAGACCCGACTCTGTCGCACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGCAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGc                         >  5:481143/1‑140 (MQ=255)
                                                                                                         aCGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAATCCGTACCGATAAAAAGATTGc                         >  3:450893/1‑140 (MQ=255)
                                                                                                         aCGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGc                         >  3:30493/1‑140 (MQ=255)
                                                                                                         aCGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACAAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTCCACCAACCCGTACCGATAAAAAGATTGc                         >  1:245619/1‑140 (MQ=255)
                                                                                                           gattatTTAAAATTTTTTCAGCCCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCa                       <  6:792502/140‑1 (MQ=255)
                                                                                                           gatcatTGACCACTTTGCCCGCCCCGCCTCTGTCGAACAAGCGCTGGACCTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGTGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCa                       <  8:104712/140‑1 (MQ=255)
                                                                                                            atgatTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTaca                                                   >  2:881092/1‑111 (MQ=255)
                                                                                                            atgatTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTaca                                                   <  1:881092/111‑1 (MQ=255)
                                                                                                             atatTGTACAATTATTCAGACCCGACTCTGTCGAACAAGAGCTGTAACTGAAGCTCCGCTACCAGGATGAAGGCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCAtt                     <  3:699091/138‑1 (MQ=255)
                                                                                                                   aaCAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTg               >  5:118454/1‑140 (MQ=255)
                                                                                                                   aaCAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTg               >  2:453436/1‑140 (MQ=255)
                                                                                                                     cAATTTATCCGACCCGCCTCTGTGGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCGGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCa             <  6:635433/140‑1 (MQ=255)
                                                                                                                     aattttttCACACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCACCGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCa             <  1:649326/137‑1 (MQ=255)
                                                                                                                     aaaTTTTTCAGCCCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCa             <  4:876134/139‑1 (MQ=255)
                                                                                                                     aaaTTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGTGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCa             <  7:354060/139‑1 (MQ=255)
                                                                                                                     aaaTTTTCCACCCCCGACTCTGTCGAACAAGCGATGGAACTGACGCGCCGCTACCAGGATGACGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCa             <  2:729584/139‑1 (MQ=255)
                                                                                                                        tttttCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCTCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGGACCGATAAAAAGATTGCCATTTCCTTGCAggt          >  1:989722/1‑139 (MQ=255)
                                                                                                                        tttttCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCAGGa          >  8:673447/1‑140 (MQ=255)
                                                                                                                          tttCATACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCAGGATc        <  2:7166/140‑1 (MQ=255)
                                                                                                                          tttCATACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCAGGATc        <  8:89031/140‑1 (MQ=255)
                                                                                                                          tttCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCAGGATc        <  3:605790/140‑1 (MQ=255)
                                                                                                                          tgtcccaCCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGGCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCAGGATc        <  7:765987/134‑1 (MQ=255)
                                                                                                                               caCCCGACTCTGTCGAACAAGCGCTGTAACTTCAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCAGGATCTGGAg   <  5:940233/139‑1 (MQ=255)
                                                                                                                               caCCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCAGGATCTGGAg   <  6:250122/139‑1 (MQ=255)
                                                                                                                                aCCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCAGGATCTGGAGc  >  6:490385/1‑140 (MQ=255)
                                                                                                                                |                                                                                                                                           
TGCGCAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGAGGGGAAATTAAGGAAAGACGATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCAGGATCTGGAGC  >  NZ_CP022686/1640797‑1641064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A