Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F2 I0 R1 193 6708.0 8620653 98.5% 8491343 136.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,678,047 A→C 25.4% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,678,0470AC25.4% 66.7 / 6.2 91intergenic (+45/‑76)agn43/CIW80_RS23450autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (52/9);  new base C (14/2);  total (79/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTGT  >  NZ_CP022686/4677909‑4678186
                                                                                                                                            |                                                                                                                                            
cACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCCTGACC‑GGGAc                                                                                                                                             >  4:989835/1‑140 (MQ=255)
  cccTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCAAGAAC‑GGGGccc                                                                                                                                           >  1:209112/1‑140 (MQ=255)
       ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCAACAAGACC‑GCGACCCGGagg                                                                                                                                      >  7:643402/1‑138 (MQ=25)
       ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTGCCCGGGC‑TCATGCCC‑GGGGCCCCGA‑Cg                                                                                                                                     >  3:355670/1‑140 (MQ=21)
       ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGACCCGGA‑Cg                                                                                                                                     >  3:783152/1‑140 (MQ=255)
       ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCAAGCCC‑CGGGCCCGGaag                                                                                                                                      >  3:420823/1‑138 (MQ=21)
       ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATGACC‑GGGACCCGGC‑Cg                                                                                                                                     >  7:689606/1‑140 (MQ=255)
       ggCGTTCAGGCCGGTTATGCCAACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCCTCATGAAC‑GGGACCCGGA‑Cg                                                                                                                                     >  3:580932/1‑140 (MQ=255)
            tCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGCCCCGGC‑Cgggggg                                                                                                                                >  1:910306/1‑139 (MQ=255)
             cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATACTGACC‑CGGACCCCGC‑CGGGGGaa                                                                                                                               >  8:378266/1‑140 (MQ=25)
             cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATAATGACC‑GGGACCCGGA‑CGGGGGaa                                                                                                                               >  4:35191/1‑140 (MQ=255)
             cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCGTCATTCCCGGGGACCGGCA‑Cgggggt                                                                                                                                >  2:924040/1‑139 (MQ=21)
             cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGACCCGGC‑CGGGGGaa                                                                                                                               >  4:427123/1‑140 (MQ=255)
             cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATAATGACC‑GGGCCCCGGC‑CGGGGGaa                                                                                                                               >  4:1012485/1‑140 (MQ=255)
                     ttATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGCAC‑GGGACCCCGC‑CGGGGGCAAACggata                                                                                                                       >  2:598415/1‑136 (MQ=25)
                          cccACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCCTCATGACC‑GGGACCCCGA‑CGGGGGAAAACGTTTAAGTGc                                                                                                                  >  4:762299/1‑140 (MQ=255)
                              caGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCAAAAAGACC‑GGGGCCCGGG‑CGGGGGGGAACGGTTAACTGCcaca                                                                                                              >  8:540875/1‑140 (MQ=21)
                               aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGCTCATGCCC‑GGGACCCGGG‑CGGGGGTAAACCGTTGAGTggcccaa                                                                                                             >  3:499741/1‑134 (MQ=25)
                               aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATAATGACC‑GGGGCCCGGA‑CGGGGGAAAACGGTTCAGGGCcacct                                                                                                             >  4:193660/1‑138 (MQ=37)
                               aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATAATGACC‑GGGGACCGGA‑AGGGGGTAAACGTTTAAGTGCcacaa                                                                                                             >  7:124615/1‑139 (MQ=25)
                                   tCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTGCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCACATTACC‑GGGACCCGGA‑CGGGGGAAAACGTTTGAGTGCCACAttaca                                                                                                         >  6:944787/1‑137 (MQ=37)
                                       cGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCGTCAAGACC‑GCGACCCGGA‑CGGGGGAAAAACTTTAAGTGCCCAATTCACAGAc                                                                                                     >  1:866149/1‑140 (MQ=25)
                                       cGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCAACAAGACC‑GGGAACCGGA‑CGGGGGAAAAACTTTACCTTCCACATTAACAGAc                                                                                                     >  1:870545/1‑140 (MQ=21)
                                         gcagcaGCGCTGAATGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCAGCAAGAAA‑CGGCCCCGGC‑CGGGGGTAAACGTTTCCGTGGAACATTAAcagccaa                                                                                                   >  5:357171/1‑134 (MQ=255)
                                           agcagtGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGAAC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCCCATAAACTGACAtta                                                                                                 >  4:115417/1‑139 (MQ=37)
                                           agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCCCGTCAACCTTACC‑CGGACCCCGA‑CGGGGGGAAACGTTTAAGTGCCCCATTAAATGACAttg                                                                                                 >  4:170957/1‑139 (MQ=25)
                                           agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGCCCCGGA‑CGGGGGAAAACGTTTCAGTGCCACCTTCACTGACATTc                                                                                                 >  4:87093/1‑140 (MQ=255)
                                           agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCGCTCTGTGGT‑CCCGGGCATCCTGCCC‑GGGCCCCCGC‑GGGGGGAAACCGTTTCAGTGCCACATTCACTGACCTTc                                                                                                 >  6:10197/1‑140 (MQ=37)
                                               gcgcTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGGCC‑GGGACCCCGA‑CGGGGGAAAACGTTTCAGTGACACATTAACTGCCATTCACaa                                                                                             >  1:846332/1‑140 (MQ=255)
                                                cgcTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GGGACCCGGA‑CGGGGGAAAACGGTTCAGTGCCCCAATCACTGACATTCACAAt                                                                                            >  8:784182/1‑140 (MQ=255)
                                                       ggTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTTATAATGACA‑GGGGCCCGGA‑CGGGGGAAAAAGTTTCAGTGACACATTCACTGACCTTCACAAGAAAATgt                                                                                     >  2:897514/1‑139 (MQ=38)
                                                          tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGTT‑CCCGGTCAGAATGACC‑GGGACCCGGA‑CGGGGGTAAACGTTTCCGTGCCACCTTAACTGACTATCACAATAACAAGatat                                                                                  >  8:807821/1‑140 (MQ=37)
                                                          tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATTACC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCAAATTCACGGACATTCACAAAAACATGatat                                                                                  >  8:680018/1‑140 (MQ=255)
                                                          tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGCCC‑GGGACCCGGC‑CCGGGGAAAACGTTTCAGTGCCCCATTCACTGACATTCACAATAACATGatat                                                                                  >  4:397171/1‑140 (MQ=255)
                                                          tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GGGCCCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACAGACATTCACAATAACATGatat                                                                                  >  4:1076982/1‑140 (MQ=255)
                                                               cAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGAAC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTTCCACATTCACTGACATTCACAATAACATGATATtaatc                                                                             >  7:640500/1‑140 (MQ=255)
                                                               cAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑CGGACCCCGA‑CGGGGGAAAACGTTTAAGTGCCACATTCACTGACATTCACAATAACATGATATtaata                                                                             >  6:337733/1‑139 (MQ=255)
                                                                 gTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GGGACCCGGC‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAAATACATGATATTCATCAc                                                                           >  6:789694/1‑140 (MQ=255)
                                                                  tCAGGCCACACTTAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACC‑GGGAACCGGA‑CTGGGGAAAACGTTTAAGTGCCACATTAAAGTAACTTCACAATAACATGATAATCATAAcc                                                                          >  6:935058/1‑139 (MQ=255)
                                                                  tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCATGACC‑CGGCCCCGGC‑CGGGGGCAAACCTTTCAGTGCCACAATCACAGAACATAAAAAAAACATGCTATTAATAACg                                                                          >  1:1060637/1‑140 (MQ=255)
                                                                  tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCAAGACC‑GGGACCCGGC‑CCGGGGTAAACGTTTAAGTGCCACATTCACTGACATTCACAATAACATGATATTAATCACg                                                                          >  3:769302/1‑140 (MQ=255)
                                                                  tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCAAGACC‑GGGACCCGGA‑CCGGGGAAAACGTTTCACGGCCCCCTTAACTGACATTCAAAATAACATTATATTCATAACg                                                                          >  2:412355/1‑140 (MQ=255)
                                                                  tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCTCAGGACC‑GGGACCCGGA‑CGGGGGAAACCGTTTCAGTGCCACAATGACTGACATTCACAATAACATGATATTAATGACg                                                                          >  4:428186/1‑140 (MQ=255)
                                                                  tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGGCC‑GGGGCCCGGC‑AGGGGGTAAACGTTTCAGTGCCCCAGACACTGGCCTTCTCAATAACAAGATATtcaaaacg                                                                          >  6:962322/1‑135 (MQ=255)
                                                                  tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GCGCCCCCGACCGGGGGAAAAC‑TTTCAGTGCCACAATAACTGACATTAAAAAAAACATGATATtaataaac                                                                          >  7:58245/1‑136 (MQ=255)
                                                                  tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTAATCAATCAC‑GGGCCCCGGG‑CGGGGGTAAAAGTTTAAGTGCCACATTGACAGACATTCACAATAACAAGATGATAATCACg                                                                          >  2:1089000/1‑140 (MQ=255)
                                                                  tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGGATCATCGCC‑GGGGCCCCGC‑CGGGGGGAATCGGATCATGGGCCCCTTCACGAACATTCACAATAACATGATATTAATCACg                                                                          >  8:658118/1‑140 (MQ=255)
                                                                  tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGACCCGGG‑CGGGGGGAAACGTTTCAGTGCCACATTTACTGACAGTCACAATAACAATATATTAATCACg                                                                          >  6:498036/1‑140 (MQ=255)
                                                                  tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCCTCATGCCC‑GGGGCCCGGA‑CGGGGGAAAACGTTTAAGTGCCCCCTTAACTGCCATTCACAATAACATGATATTAATAACg                                                                          >  2:1093734/1‑140 (MQ=255)
                                                                  tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCCTCATGACC‑GGGCCCCGGG‑CGGGGGAAAACGGTTCCGAGCCACCTTCACTGGCATTAACAAAAACATGATATtcatgaag                                                                          >  4:1071983/1‑136 (MQ=255)
                                                                          catttattgtgAACTTTTTATAATTTTTCATTCCCTCTGTGTGGT‑CCTGGTCATAATTCCG‑GGTACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACt                                                                  <  6:798788/133‑1 (MQ=255)
                                                                          cacTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTAATACCGGAgggcct                                                                  >  8:340662/1‑135 (MQ=255)
                                                                          atcttaaTGTGACATTCTGATATTTTTGCATTGCCTCTGTGGGGC‑CCGGGTCATCATGACC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACt                                                                  <  8:124615/135‑1 (MQ=255)
                                                                          aacttattgtgACCTTCTTATATTTCTCCATTGCCTCTCGGGGGC‑CCCGTGCCTCATGCCC‑GGCACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACt                                                                  <  6:509338/133‑1 (MQ=255)
                                                                           acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATTACC‑GGGCACCGGC‑CGGGGGCAAACGGTTGAGGGGCACCTTCACTGACATTCACAATTACATGAGATTCATCACGGAGTGagtt                                                                 >  5:74285/1‑137 (MQ=255)
                                                                           acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGATAATGACC‑GGGACCCGGA‑CGGGGGGAAACGTTTCAGTGGCCCATTAACTGACATTCACAATAACATGATATTAATAACGGAGTGACTa                                                                 >  4:521875/1‑140 (MQ=255)
                                                                                     aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGATCATGTCC‑CGGACCCCGA‑CGGGGGCAAACCGTTCAGTGCCACAATAACAGACATTCACAATAACAACATAATAAACACGGAGTTACAATTTTaaataa                                                       >  2:199114/1‑135 (MQ=255)
                                                                                       cTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATTACC‑GGGCCCCGGC‑CGGGGGCAAAACTTTCAGTTCCACATTCACTGCAATTCACAATAAAATGATATTAATCAAGGAGTGGATATGTTAAACATat                                                     >  7:438464/1‑139 (MQ=255)
                                                                                       cTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTAATCATGACC‑CGGACCCGGA‑CGGGGGAAAACGTTTAAGTGCCACATTCACAGACATTCACAATAACATGATATTAATCACGGAGTGCCTATGTTAAAAATAg                                                     >  8:452979/1‑140 (MQ=255)
                                                                                       cTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACC‑GGGGCCCGGA‑CGGGGGGAAACGTTTAAGTGGCACATTCACAGACCATAACAAAAAAATGATATTAATAACGGAGTGACTTTGTTTCAGAttt                                                     >  5:325004/1‑138 (MQ=255)
                                                                                         tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGCACATTACC‑GGGACCCGGG‑CGGGGGAAAACGGTTCCGTGCCACAGTCAAGGACAATAACAAAAAAATGATATATATCACGGAGGGACTAATTTAGAAATAGta                                                   >  6:174560/1‑139 (MQ=255)
                                                                                         tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GGGACCCGGA‑CGGGGGTAAACGGTTCAGTGCCACATTAACTGACATTAACAATAACAATATATTAATAACGGAGTGACTATGTTACAGATatta                                                   >  3:986983/1‑137 (MQ=255)
                                                                                         tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTAATCATGACC‑GGGACCCGGA‑AGGGGGAAAAAGTTTCAGTTACACATTAACTGACATTCACAATTACATGATATTAATCACGGATTTACTATTTTAAAGATattt                                                   >  2:663407/1‑137 (MQ=255)
                                                                                         tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGGATCATGAAC‑CGGACCCGGG‑CGGGGGAAAAAGTTTACGTTCCAAATTCACAGGCATTAAAAATAACATTAAATTAATCAAGGATGGACAATGTTACAtatattg                                                   >  2:784796/1‑137 (MQ=255)
                                                                                         tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGCCCCGGA‑CGGGGGGAAACGTTTAAGTGCCCCATTAACTGACATTCACAATAACATTATATTAATAACGGAGTGACTATGTTACAGATAGta                                                   >  7:1000890/1‑139 (MQ=255)
                                                                                         tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCCTCATGACC‑GGGACCCCGA‑CGGGGGAAAACGTTTAAGTTCCACATTCACAGCAATTAACAAAAACATGATATTAATAAACGAGTGACTATTTTACAGATAGta                                                   >  1:1078527/1‑139 (MQ=255)
                                                                                         tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGGATCATGACC‑GGGGACCGGG‑CGGGGGAAAACGTTTTAGGGCCACCTTAACGGACATTAAACATAAAATGTTTTTCATCAAGGGGTGCCTATTGTACAGAGAGtg                                                   >  5:772458/1‑139 (MQ=255)
                                                                                         tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCCTCATGACC‑GGGCCCCCGC‑CGGGGGAAAACGTTTCAGTTCCACAATAACGGACATTAACAATAAAATGATATTAATCACGGTGTGCCTATGTTTCAGaatgta                                                   >  2:353372/1‑135 (MQ=255)
                                                                                         tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATAAAGACC‑CGGGCCCCGA‑CGGGGGAAAAACGTTCAGTGGCCCAATCACAGACATTAACAATAACATGATATTAATAACGGAGTGTCTATGTTTAAGATAGTc                                                   >  5:485640/1‑140 (MQ=255)
                                                                                           ttaaaaTTCTGATTGGCCTCCGTGGGGC‑CGCGGTCATCATGCCG‑GGGCCCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCgg                                                 <  2:1010512/136‑1 (MQ=255)
                                                                                                    tGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACC‑GGGACCCGGA‑CCGGGGAAAACGGTTCAGTGCCACATTCACTGACAATCCCAATAACAAGATATTAATAAAGGAGTTAATATGTTACAGATATTAGGCGCGCTTAt                                        >  6:944924/1‑140 (MQ=255)
                                                                                                              ctctGGTGT‑CCCGGCCCACACGACG‑GGGCCCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATc                              <  6:949396/140‑1 (MQ=255)
                                                                                                                 tgtgGG‑CCCGGGCATCATGACC‑GGGGCCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCa                           <  8:250739/140‑1 (MQ=255)
                                                                                                                         cccgTCATCAAGACC‑GGGACCCGGA‑CGGGGGAAAAAGTTTCAGTGCCACATTAACTGACATTCAAAATAAAATTATATTAAAAAAGGATTGACTATTTTTCAGATATTAGGCGCGCGGCTTCTTCTGATAGCAGGAtttt                    >  2:544700/4‑139 (MQ=255)
                                                                                                                                   ccgccggggACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACAGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttcttc           <  7:429537/134‑1 (MQ=255)
                                                                                                                                     aCC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCTCAGGATTTGCCATTCTTCGcc        >  4:1036508/1‑140 (MQ=255)
                                                                                                                                      cc‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATAGCAGGATTTTCAATTCTTCGCCt       >  8:139798/1‑140 (MQ=255)
                                                                                                                                       c‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATtcatca                                                                            >  6:633423/1‑70 (MQ=255)
                                                                                                                                       c‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATtcatca                                                                            <  5:633423/70‑1 (MQ=255)
                                                                                                                                       c‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGa                                         <  7:591596/105‑1 (MQ=255)
                                                                                                                                       c‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGa                                         >  8:591596/1‑105 (MQ=255)
                                                                                                                                        ggggACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtt      <  4:965341/139‑1 (MQ=255)
                                                                                                                                        gggcaCCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtt      <  8:480993/136‑1 (MQ=255)
                                                                                                                                          ggACCCGGC‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtttt    >  1:265967/1‑140 (MQ=255)
                                                                                                                                          ggACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGa                                                                    >  3:727848/1‑76 (MQ=255)
                                                                                                                                          ggACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGa                                                                    <  4:727848/76‑1 (MQ=255)
                                                                                                                                          ggACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGcc                  <  3:398335/126‑1 (MQ=255)
                                                                                                                                          ggACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGcc                  >  4:398335/1‑126 (MQ=255)
                                                                                                                                          ggACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtttt    >  8:431263/1‑140 (MQ=255)
                                                                                                                                          ggACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtttt    >  5:1048353/1‑140 (MQ=255)
                                                                                                                                          ggACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtttt    >  5:836949/1‑140 (MQ=255)
                                                                                                                                           gACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTg   >  1:930182/1‑140 (MQ=255)
                                                                                                                                            aCCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTGt  >  5:152602/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACC‑GGGACCCGGA‑CGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTGT  >  NZ_CP022686/4677909‑4678186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A