Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F13 I0 R1 230 5035.0 9125430 98.4% 8979423 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,678,047 A→C 24.5% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,678,0470AC24.5% 73.3 / 2.3 90intergenic (+45/‑76)agn43/CIW80_RS23450autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (48/12);  new base C (11/4);  total (74/16)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTG  >  NZ_CP022686/4677914‑4678185
                                                                                                                                       |                                                                                                                                           
tGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑ACCCggc                                                                                                                                       >  6:312422/1‑139 (MQ=255)
  ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATATCAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑ACCCGGaag                                                                                                                                     >  2:481662/1‑138 (MQ=255)
  ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGGATCAGGACCGGG‑ACCCGGCCg                                                                                                                                     >  5:265719/1‑140 (MQ=21)
  ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGCTCATGCCCCGG‑ACCCggc                                                                                                                                       >  8:757873/1‑137 (MQ=255)
  ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGAGGT‑CCCGGGCAACAAGACCGGG‑ACCCGGACg                                                                                                                                     >  2:852573/1‑140 (MQ=255)
  ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCCCTGTGGT‑CCCCGGGATCATTACCGGG‑GCCCCGAcc                                                                                                                                     >  5:524158/1‑139 (MQ=21)
  ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGAATTGCCTCTCTGTGGT‑CCCGGTCATCAAGACCGGG‑CCCCGGACg                                                                                                                                     >  5:266266/1‑140 (MQ=255)
  ggCGTTCAGGCCGGTTATGACCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGCCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGACAACCAGCCCGGG‑ACCCgggcc                                                                                                                                     >  5:101267/1‑137 (MQ=21)
    cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACg                                                                                                                                     >  7:723301/1‑138 (MQ=255)
    cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCCAAATGACCGGG‑GCCCGGACg                                                                                                                                     >  7:517814/1‑138 (MQ=21)
      ttCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATAAAGACCGGG‑GCCCGGCCggggg                                                                                                                                 >  3:369404/1‑140 (MQ=25)
       tCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCAAAATGACCGGG‑CCCCGGACGGGGGa                                                                                                                                >  6:862649/1‑140 (MQ=255)
       tCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑ACCCGGACGGGGGa                                                                                                                                >  6:343782/1‑140 (MQ=255)
        cAGGCCGGTTATGCCCACAGCATCACCGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATTCCCGGG‑CCCCCGAAGGGGGag                                                                                                                               >  3:744836/1‑139 (MQ=21)
        cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGCAGGTTATAACAGTCTGGCCACCCTGAAGGGGCCCGTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCAGAATGACCCGG‑GCCCGGGCGGGGGac                                                                                                                               >  5:1100775/1‑139 (MQ=25)
        cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCATGACCGGG‑ACCCGGCCGGGGGaa                                                                                                                               >  5:263148/1‑140 (MQ=255)
        cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCCTTACCCGG‑ACCCCGTCgggggca                                                                                                                               >  1:644861/1‑138 (MQ=25)
        cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATTTACGGG‑ACCCGGCCGGGGGaa                                                                                                                               >  3:744829/1‑140 (MQ=255)
                 tATGCCCACAGCATCAACGGCAGCATCGCTGAAGGTTATTACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGTTCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCa                                                                                                                      <  3:152803/140‑1 (MQ=255)
                       cacaGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCAGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCca                                                                                                                >  5:553191/1‑140 (MQ=255)
                       cacaGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATTCCCGGG‑ACCCGGACGGGGGAAAACCtttaatagc                                                                                                                  >  8:542792/1‑132 (MQ=25)
                          aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCcacaa                                                                                                             >  2:1104023/1‑139 (MQ=255)
                          aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑CCCCGGACCGGGGAAAAACTTTAAGTGCCACAt                                                                                                             >  1:753635/1‑140 (MQ=25)
                            cATCAACGGCCGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCCGG‑CCCCGGCCGGGGGCAAACGTTTGAGTGGCACAt                                                                                                             >  7:199590/1‑138 (MQ=37)
                            cATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGGCA‑GATGGACGGG‑ACCCCGGCCGGGGGAAACGGTTGAGTTCCCCAt                                                                                                             >  7:243088/1‑138 (MQ=18)
                            cATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGCCCGGG‑ACCCGGCCGGGGGAAAACGTTTCAGTGCCACAt                                                                                                             >  8:742474/1‑138 (MQ=255)
                             aacAACGCCAGCCGCGCTTATGGTAAAAACTGTCCGCCCAAACTCAATGTCACCTTCTTAAAATTCTCCATTCCCTGTCTGTGGC‑CCCGGTCATCTTGCCGGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCa                                                                                                          <  2:838806/138‑1 (MQ=255)
                                      agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACGGGGGAAAAAGTTTGAGTGCCACATTCACTGACAtta                                                                                                 >  5:694672/1‑139 (MQ=255)
                                        cagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCCGG‑ACCCGGGCGGGGGAAAACGTTTCAGTGCCACAGTCACTGACATTcac                                                                                               >  2:888844/1‑140 (MQ=255)
                                        cagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGGCTCATTGCCCGG‑GCCCGGGCGGGGGGAAACGGTTTAGTGCCCCAGTGACTGACAtta                                                                                                 >  8:222998/1‑137 (MQ=21)
                                        cagcGCTGAAGGTTATAACAGTAAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGCTCCAGCCCGGG‑CCCCGGACGGCGTAAAACGTTTAACTGGCACATTCACTGaaaatg                                                                                                 >  8:250605/1‑133 (MQ=25)
                                                     tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCCGGGCAACATGACCGCG‑CCCCGGGCGGGGGAAAACAATTAAGTTCCCCAATACCAGAAATAAACAAAAACA‑TGaaat                                                                                 >  6:233993/1‑140 (MQ=255)
                                                       taACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGGCCGGG‑ACCCGGGCGGGGGAAAACGTTTCAGTGGCACAGTAACTGACATTCACCATAACA‑TGctat                                                                                 >  7:255954/1‑138 (MQ=255)
                                                         aCAGACCGGCCACACTCAATGTCACATTCTGAAATTTCTGCATTGCCTGTGTGTGGC‑CCCGGTCACCA‑GCCCGGGCCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATtcat                                                                             <  4:1111318/140‑1 (MQ=255)
                                                          cAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCTCATGACCGGG‑ACCCGGGCGGGGGAAAACGGTTCAGTGCCACATTCACTGACATTCACAATAACA‑TTATATtaata                                                                            >  6:473292/1‑139 (MQ=255)
                                                             tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑GCCCGGCCGGGGGAAAACGTTTAAGTGCCACATTCACTGACATTCACAATAAAA‑TGATATTCATCAcc                                                                         >  1:888488/1‑139 (MQ=255)
                                                             tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACg                                                                         >  6:1006608/1‑140 (MQ=255)
                                                             tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCCTCATTACCGGG‑GCCCGGACGGGGGAAAACGGTTCAGTGGCCCATTCACTGACCTTAACAAAAACA‑TGATATTAATCACg                                                                         >  1:608423/1‑140 (MQ=255)
                                                             tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCCACATGCCCCGG‑CCCCGGCCGGGGGAAAACCTTTCAGGGCCCCATTCACTGACATTCACAAAAACA‑TGATATTCATcgcg                                                                         >  3:1037652/1‑140 (MQ=255)
                                                             tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCAAGCTTACCGGG‑GACCCGACGGGGGAAAACCTTTAAGTGCCACAATCAATGTCATTAACACAAACA‑TTAGATTAATAACg                                                                         >  1:749052/1‑140 (MQ=255)
                                                             tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGCTCATGACCCGG‑ACCCGGCAGGGGGTAAACGTTTCAGTGCCACAGTCACTGACATTCACCATTACA‑TGATTTtaatcaaa                                                                         >  1:1035661/1‑138 (MQ=255)
                                                             tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTACTCATGACCGGG‑GCCCGGGCGGGGGAAAACGGTTCAGTGCCCCCTTCACTGACCTTAACAATAACA‑TGTAATTCATCACg                                                                         >  6:262310/1‑140 (MQ=255)
                                                              cAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCAGGTCATCAAGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTAACAATAACA‑TGATATTCATCACgc                                                                        >  6:913133/1‑139 (MQ=255)
                                                               aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑GCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAAAA‑TGATATTCATCAcc                                                                         >  7:156867/1‑137 (MQ=255)
                                                                 gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATTACCGGG‑TCCCGGAACGGGGCAAAAGTTTGAGTTGCCCCTTCAGAGTCAATGACAATAACA‑ATATTTTACTCCCGGAGt                                                                     >  5:537731/1‑140 (MQ=255)
                                                                 gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGGATCAAGACCCGG‑CCCCCGCCGGGGGAAAAAGTTTGAGTGGCCCATTAAATGACATTGCCAATAACA‑TGATTTTAAACAAGGAGt                                                                     >  3:1010069/1‑140 (MQ=255)
                                                                 gCCACACTCAATGTGACCTTCTGATAATTCTGCATGCCCTCTGTGTGGC‑CCCGGTAATCATCACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGGGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGa                                                                       <  8:168427/138‑1 (MQ=255)
                                                                   cacacaGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTAATCATGCCCGGG‑ACCCGGAAGGGGGAAAACGTTTCAGTGCCACATTCACGGACATTAGCAATAACA‑TGATATTCATCACGGAGt                                                                     >  8:645182/1‑138 (MQ=255)
                                                                   cacaCTTATTGTGACATTCTTATATTTCTGCATTGCCTCTCTGTGGG‑CCCGGTCATCATCCCGGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGt                                                                     <  8:723301/138‑1 (MQ=255)
                                                                     cacTTAATGTGACATTCTGAAAATTCTGCCTTCCCGCTCTGGGGT‑CCCGGGCAAACTGCCCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACt                                                                 <  4:691060/140‑1 (MQ=255)
                                                                     cacTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATGACCGGG‑GCCCGGACGGGGGAAAACGTTTGAGTGGCACATTCACGGACATTCACAATAACA‑TGATATTAATAACGGAGTGACt                                                                 >  3:64249/1‑140 (MQ=255)
                                                                      acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCAAGACCGGG‑ACCCGGGGCGGGGAAAACGTTTAAGGGCCACATTCACAGAGATTCACAATAAAA‑TTATATTAATAACGGAGTTAcaa                                                                >  4:371245/1‑138 (MQ=255)
                                                                          aaTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGtt                                                            >  4:638988/1‑140 (MQ=255)
                                                                                aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCCCGTCCTTCTTACCGGG‑ACCCGGGAGGGCGCAAACGTTTGACTGACCCAAAGACTGGAATT‑ACCAAAACATTTATATTAATCCCGGATGGACAATGTTACactt                                                      >  2:39753/1‑137 (MQ=255)
                                                                                aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATTACCCGG‑ACCCGGCCCGGGGAAAACGTTTCAATGCCCCATTACCAGACATTCACAAAAACA‑AGATATTAATCAAGGCATTACTATGTTACAAAt                                                      >  6:339695/1‑140 (MQ=255)
                                                                                 ccTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATTACCGGG‑ACCCGGCCGGGGGGAAACGTTTAAGTGCCACAGTCACTGGCATTCAAAATAACA‑ATATAATAAAAACGCCGTGAGCATGTTACAGa                                                       >  8:139482/1‑138 (MQ=255)
                                                                                  cTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑ACCCGGACGGGGGATAACGTTTCAGTGCCCCATTGACTGCCATTCACAATAACA‑TGATATTAATCACGGAGTGACTATGTTACAGATat                                                    >  6:1014641/1‑139 (MQ=255)
                                                                                    tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCAATACCCGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCCCATTCCCAGCCATTAACAATAAAA‑AAATATTAAACACGGGGGTACGATTTTACAAATAGtg                                                  >  2:383529/1‑139 (MQ=255)
                                                                                    tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCCTAATGACCCGG‑ACCCCGCCGGGGGAAAACGTTTAAGTTCCCCAATCACAGACAATCACAAAAAAA‑AGATATTAATCAACGAGTGACTATTTTTCAGATatta                                                  >  6:219839/1‑137 (MQ=255)
                                                                                    tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTAATAAATACCCGG‑ACCCAGACGGGGGAAAACATTTCATTGCCCCATTCACTGACAATAACAAAAACA‑TAAAAATAATAACCGAGTGACTATTTTACAGATAGTc                                                  >  3:466709/1‑140 (MQ=255)
                                                                                    tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGGATCATGACCGGG‑GACCGGGCGGGGGGAAAAGGTTAAGTGCCACATTAACTGACATTCACAATAAAA‑TGATATTAATAACGGAGTGACTATGTTTAAGATAGta                                                  >  6:704868/1‑139 (MQ=255)
                                                                                    tCTGAAAATTCTGCATTGCCTCTCTGTCGT‑CCCCGGAATCAAAAACGGG‑ACACGGAAGGGTGAAACAGATTCAGTGCCACAATCACAGACATTAACAATAACA‑TCATATTAATCTCGGAGAGACTATGTTACAGaatgta                                                  >  4:900303/1‑135 (MQ=255)
                                                                                      ttaTAATTCTCCATTGCCGCTCTGGGGG‑CCGGGTCATCCGGCCGGGG‑CCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCgg                                                <  4:862857/138‑1 (MQ=255)
                                                                                      tGATAATTCTGCATTGCCTCTCTGTGGTGCCCGGGC‑TCATGGCCCGG‑GCCCGCGCGGGGGGAAACGGTTAAGGGTCACCATAACAGACAATAACAATAACA‑ATATATTAAACACGGCGTGGGAATGTTAAAGaaattg                                                  >  8:782619/1‑133 (MQ=255)
                                                                                      tGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTAACTGACATTCACAATAACA‑TGATATAGATCACGGAGTGACTATGTTTCAGATTGtg                                                  >  8:677347/1‑137 (MQ=255)
                                                                                      tGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGATAATGACCGGG‑ACCCGGCCGGGGGAAAACGTTTGAGTGCCACCATAACTGACAATCACAATAACA‑TGATATTAATAACGGAGTGACATTTTTACAGATATTCgg                                                >  4:742619/1‑140 (MQ=255)
                                                                                      tGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑GCCCCGGCCGGGGATAAAGTTTCAGTGGCCCAATCACTGACAATCCAAATAACA‑TGAGATTACTCACGGGGTGAAGATGTTTCAGATAGtg                                                  >  8:372663/1‑137 (MQ=255)
                                                                                      tGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATAATGACCGGG‑ACCCGGCCGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTAAAGATAGta                                                  >  8:185808/1‑137 (MQ=255)
                                                                                              ttctaTTGCCTGTCTGTGCC‑CCCGGACATCATCCCCGGT‑ACCCTGAAGGTGTAAAACGTTTCAGTGCCAAATTCACTGACTTTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCt                                          <  7:549954/134‑1 (MQ=255)
                                                                                                gCATTGCCTGTGTGTTGT‑CCCGGTCATCATCACCGGT‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGAtt                                      <  4:643096/140‑1 (MQ=255)
                                                                                                        tctctGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGTCGGGGGAAAACGTTTAAGTGCCCCATTCACTGACATTAACAATAACA‑TGATATTAATCACGGAGTGACTATGTTACTGATATTCGGCGCGCTGATTctggtg                                >  8:253922/1‑138 (MQ=255)
                                                                                                         ctctGTGGT‑CCCGGTCATAATGACCGGG‑CCCCGGACGGGGGAAAACCTTTCATTTCCACATTAAAATAAATTCACCATAAAA‑TGAAAATAATAAATGTGTGCCTATGTTACAACTAGTCGGCCTCCTGATTatggagagg                             >  4:444382/1‑134 (MQ=255)
                                                                                                           ctGTGGT‑CCCCGTGATCATGACCGGG‑CCCCCGGCGGGGGGAAACATTTAAGTTCCCCATTAACTGCAATTCACAATAACA‑TGATATTAATAACGGAGTGACTATGTTAAAGATATTAGGCGCGCAGAATCTGCTGCTCGc                           >  2:108508/1‑140 (MQ=255)
                                                                                                                 t‑CCCCGTGATAATGACCCGG‑ACCCGGACGGGGGAAAACGTTTAATTGCCCCATTCACATACATTAACAAAAACA‑ATATATTAATAAACGACTGAATATGTTAAAGAAATTAGGCGCGCTGATTATGCTGATAGCAGGAtt                     >  6:840383/1‑140 (MQ=255)
                                                                                                                      ggTCATCATGCCCGGC‑CCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGcc                 <  4:164894/140‑1 (MQ=255)
                                                                                                                           tcatGACCGGG‑ACCCGGACGGGGGAAATCGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTTATCGAAGGATTTGCCAttct            >  1:852064/1‑140 (MQ=255)
                                                                                                                           taattaCCGGG‑ACACGGACGGGGGAAAACTTTTCAGTTCCACAGTCACTGACATTCACAATAACA‑TGATATTCATAACTGAGAGAATATGTTAAAGATAGTCGGCGCGCTGATTCTGCTGATCGCATGAATTGCAAttcg            >  1:852059/6‑139 (MQ=255)
                                                                                                                             tgccggggg‑CCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttct            <  7:836500/132‑1 (MQ=255)
                                                                                                                             attaCCGGT‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttct            <  8:134291/135‑1 (MQ=255)
                                                                                                                             atGACGGGG‑ACCCGGACGGGGGAAAACGTATCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttcttc          <  4:859580/140‑1 (MQ=255)
                                                                                                                              tGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGACATTCTTCg         >  3:1046551/1‑140 (MQ=255)
                                                                                                                                  gggg‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtt     <  5:1006608/139‑1 (MQ=255)
                                                                                                                                  gggg‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtt     <  6:443596/139‑1 (MQ=255)
                                                                                                                                  cGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCcaca                                                                                                              >  4:157668/1‑36 (MQ=255)
                                                                                                                                  cGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCcaca                                                                                                              <  3:157668/36‑1 (MQ=255)
                                                                                                                                    gg‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCGGGAATTGCCATTCTTCGACtttt   >  1:873523/1‑140 (MQ=255)
                                                                                                                                    gg‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTTCCATTCTTCGCCtttt   >  6:19531/1‑140 (MQ=255)
                                                                                                                                    gg‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtttt   >  5:602396/1‑140 (MQ=255)
                                                                                                                                     g‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTg  >  3:369092/1‑140 (MQ=255)
                                                                                                                                     g‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTg  >  1:638720/1‑140 (MQ=255)
                                                                                                                                       aCCCGGACGAGGGAAAACGTAACAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTAAATAAAAGAAAGACGGCGCGCTGATTCTGCTGATCGCCGGAATTGCCATACTACGACattt   >  7:512267/1‑138 (MQ=255)
                                                                                                                                       |                                                                                                                                           
TGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑ACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACA‑TGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTG  >  NZ_CP022686/4677914‑4678185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A