Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F35 I0 R1 195 10216.4 8585726 98.3% 8439768 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,678,047 A→C 22.7% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,678,0470AC22.7% 63.2 / 3.2 90intergenic (+45/‑76)agn43/CIW80_RS23450autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (51/10);  new base C (11/5);  total (74/16)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTGT  >  NZ_CP022686/4677911‑4678186
                                                                                                                                          |                                                                                                                                            
cccTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGcccc                                                                                                                                           >  4:501861/1‑140 (MQ=255)
     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTGATCCTG‑ACCCGGGCCCGGCCg                                                                                                                                      >  8:720736/1‑140 (MQ=21)
     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCAAAATG‑ACCGGGACCCGGAcc                                                                                                                                      >  1:593459/1‑139 (MQ=255)
     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGCATCATG‑ACCGGGACCCGGAcc                                                                                                                                      >  7:148663/1‑139 (MQ=255)
     ggCGTTCAGGCCGGTAATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGAATAATG‑ACCGGGAccccgc                                                                                                                                        >  6:2183/1‑135 (MQ=25)
         ttCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGACggggg                                                                                                                                  >  8:765565/1‑140 (MQ=255)
         ttCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGCATCATG‑CCCCGGACCCCGACggggg                                                                                                                                  >  8:9578/1‑140 (MQ=25)
           cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGGCGGGGGaa                                                                                                                                >  4:853925/1‑140 (MQ=255)
           cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATCATG‑ACCGGGACCCGGACGGGGGaa                                                                                                                                >  1:1000968/1‑140 (MQ=255)
             ggCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATCATG‑ACCGGGACCCGGACGGGGGaa                                                                                                                                >  6:701070/1‑138 (MQ=255)
                  gTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTGCTGTTAATTATGCATTG‑CCTCTCTGTGGTCCCGGGATTCATG‑ACCGCGACCCGGCCGGGGGCAAACGttt                                                                                                                         >  3:574799/1‑140 (MQ=25)
                   ttATGCCCACAGCCTCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCATT‑ACCTGGACCCCGCCCGGGGCAAACgggta                                                                                                                        >  7:990363/1‑136 (MQ=21)
                   ttATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATCATG‑ACCGGGACCCCGGCGGGGGGAAACGtttg                                                                                                                        >  7:542406/1‑139 (MQ=255)
                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCTCACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCCTCATT‑ACCGGGGCACGGCCGGGGGTAAAAGTTTCAG‑TGccccct                                                                                                             >  2:709225/1‑136 (MQ=25)
                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGCCCCGGACGGGGGAACACGTTTCAG‑TGCcccaa                                                                                                             >  2:783948/1‑139 (MQ=255)
                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCcacaa                                                                                                             >  2:633392/1‑139 (MQ=255)
                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCCTCAGG‑ACCGGGCCCCCGGCGGGGGGAAACGGTTCAG‑TGCCCCAt                                                                                                             >  2:299328/1‑140 (MQ=25)
                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCCACATT‑ACCCGGCCCCGGCCGGGGGGAAACGTTTCAG‑TGCcacaa                                                                                                             >  3:951804/1‑139 (MQ=25)
                               cATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCATT‑ACCGGGCACCGGCCGGGGGTAAACGTTTAAG‑TGCcacaa                                                                                                             >  5:1049678/1‑137 (MQ=37)
                               cATCAACGGCAGCAGCGCTGAAGGTTATAAAAGTCAGGCCACACTGAATGTGACCTTCTGATAATTATGCATTT‑CCTCTCTGTGGTACCGGGGATCATT‑ACCGGGAACCTGACGTGTGAAAACTTTTAAT‑TGcccaat                                                                                                             >  6:458979/1‑134 (MQ=21)
                                aTCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGGATCATG‑ACCGGGGCCCGGGCGGGGGGAAACGGTTGAG‑TTCcccaat                                                                                                            >  5:397433/1‑136 (MQ=25)
                                 tCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATAATG‑ACCGGGACACGGACGGGGGAAAAAGTTTCAG‑TGCCACATTCAc                                                                                                         >  4:818345/1‑140 (MQ=255)
                                     cGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGATCCCGGTCATCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGAc                                                                                                     <  8:503219/140‑1 (MQ=255)
                                      gccagccgCGCTGATGTTTATAACAGCCCGCCCACCCTTAAGGTGACCTTCTTAAAATGCTGCATTC‑CCGCGCGGGGGCCCCGGCCAACATC‑CCCGGCACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACa                                                                                                    <  4:430169/138‑1 (MQ=255)
                                       gcagcaGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTAATAATA‑GCCGGGCCACCGCCGGGGGAAAAACTTTCAG‑TGCCACATTCACAGACAt                                                                                                   >  3:33587/1‑140 (MQ=25)
                                         agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATAATG‑ACCGGGACCCGGACGGGGGTAAAAGTTTGAG‑TTCCACATTAAcaggcaa                                                                                                   >  6:700746/1‑132 (MQ=25)
                                         agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATCATG‑GCCGCGCCCCGGGCCGGGGAAAACGGTTCAT‑TTCCACCTTCACTGACaata                                                                                                 >  3:200569/1‑137 (MQ=25)
                                             gcgcTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATACTG‑ACCGGGACCCGGACGGGGGAAAACGTTTAAG‑GGCCACATTCACTGCAATTcac                                                                                               >  5:971380/1‑138 (MQ=255)
                                             gcgcTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCAGTAAACATT‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGACAAAATCACTGACAtaaac                                                                                               >  5:1023371/1‑134 (MQ=255)
                                                cTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCCTAAAG‑ACCGGGACCCGGACGGGGGAAAACCTTTGAC‑GGCCCCAGTAACAGCCAATCACAATaa                                                                                          >  1:45932/1‑140 (MQ=255)
                                                  tatgtttATAACTGTCTGGCGACCATTAATGAGACCTTCTGATAATTCAGAATTG‑ACTCTGTGGGGTCCAGCTCATCTAG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACa                                                                                        <  2:979989/135‑1 (MQ=255)
                                                  gAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTGATCATGAACCGGGA‑CCGCTCGTGGGAAAACGTTTCAG‑TGCCCCATTCACTGACCTTCACAATaa                                                                                          >  6:313942/1‑138 (MQ=37)
                                                  gAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTAACTGACATTCACAATaa                                                                                          >  6:70721/1‑138 (MQ=255)
                                                  ctggtttAAAACTGTCCGCCCACACTTATTGTCACTTTATTATATTTCTG‑ATTGCCCTCTGGGGGGCCCGGGTCAACATG‑CCGGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACa                                                                                        <  2:1026205/135‑1 (MQ=255)
                                                   aaGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCCTTG‑CCTCTCTGTGGTCCCCGTCAGCATT‑ACCGGGACCCGGACGGGGGAACACGTTTCAG‑TTCAACATTCTCTTGCATTCACACTAACAt                                                                                       >  4:279096/1‑140 (MQ=255)
                                                     ggTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCAAT‑CCCGGGACCCGGCCGGGGGTCAACGTTTAAG‑TGCCACAATCACTGACAATGACAATaaaaa                                                                                       >  5:541423/1‑135 (MQ=37)
                                                     ggTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGCATCATG‑ACCGGGGCCCGGGCGGGGGGAAACGGTTGAGTTGCCAC‑TTCCCTGCCATTCACAAAAAAATGa                                                                                     >  3:508230/1‑140 (MQ=25)
                                                       ttATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCAGTGATAATG‑ACCCGGGCCCGGACCGGGGAAAAAGTTTAAG‑TGGCCCAATCACTGACAATAACAAAAACaata                                                                                     >  5:197900/1‑135 (MQ=255)
                                                        tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGGATAATG‑TCCGGGTCCCCGCCGGGGGGAAACGGTTCAG‑TGCCCCCTTGTCAGCCATTAACATTAAAATGttat                                                                                  >  1:216128/1‑140 (MQ=255)
                                                         ataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCTTCATG‑ACCGGGACCCGGGCGGGGGATAACGGTTCAG‑TGACACATTCACGGACATTCACAATAACATGTTAtt                                                                                 >  1:861901/1‑140 (MQ=255)
                                                            aCAGTCAGGCCACACTGAATGTGACCTTCTGATAATGCTGCATTG‑CCTCTCTGTGTTCCCGGTCATCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATtc                                                                                <  6:73132/138‑1 (MQ=255)
                                                            aCAGTCAGGCCACACTGAATGTGACCTGCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGCATCCTG‑ACCGGGACCCGGGCGGGGGAAAACGGTTCAG‑TGCCACATTCACTGAAATTCCCAATAACATGAtttta                                                                                >  6:182180/1‑137 (MQ=255)
                                                              ttcctcgCCACATTAATTGTTACTTTCTTATATTTCTGATTTG‑CCTCTTGGGGGCCCGGGTAATCTTC‑CCGGGCACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATtcat                                                                              <  6:415890/132‑1 (MQ=255)
                                                                tccgcccACAGTTAATGTGACCTTCTAAAAATTCTCCATGC‑CCTCTCTGGGGCCCCGGTCATCATC‑CCGGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACg                                                                          <  8:812961/135‑1 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTGCCCGGCGTCATG‑ACCGGGGCCCCGACGGGGGTAAACGTTTCAG‑TGCCACAATCACTTCCAATAACAATAAAATCATATTGATAACg                                                                          >  1:858518/1‑140 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTGATCATG‑ACCGGGACCCGGACGGGGGGAAACGTTTCAG‑TGCCACCATCACTGACATTAACAATAACATGATATtcatacag                                                                          >  1:1026205/1‑136 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑CCCGGGCCCCGGGCGGGGGAAAACGGTTCAG‑TGCCCCATTGACTGCCAGTCACAATAACATGATATTCATCACg                                                                          >  2:810984/1‑140 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACg                                                                          >  4:661061/1‑140 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGGATCCTG‑ACCGGGCCCCGGCCGGGGGGAAACGTTTATG‑TGCCACCTTAACTGCCAGTCACAATAACATGAGATtaatacag                                                                          >  1:682558/1‑136 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATCATG‑GCCGGGTCCCGGCCGGGGGGAAACGGTTCAA‑GTCCACCTTCACGGAAATTGCCCATAACAAGATATTGATCAct                                                                          >  7:1012956/1‑139 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATCATG‑AACGGGACCCGGACGGGGGAAAAAGTTTCAG‑TGGCACCTTAAAAGACTTTCACAATAACATGATATTCATCACg                                                                          >  3:782671/1‑140 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGAATCCTG‑ACCGGGACCCGGACCGGGGAAAACGTTTCAG‑TGCCACATTAACGGGCATTAACAATAACAAGATATTCATCACg                                                                          >  3:431912/1‑140 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTACCCGTGATAAAT‑CACGGGACCCGGACGGGGGAACACGGTTAAG‑TGCCACAATAACAGACAAAGACAAAAACATGATAttgaaaacc                                                                          >  8:286641/1‑133 (MQ=255)
                                                                  aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGACGGGGGAAAAAGTTTCAG‑TGCCACCATCACTGACATTCCCAATAAAATTATAATAAACACGGa                                                                        >  4:836759/1‑140 (MQ=255)
                                                                  aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGCATCAAG‑ACCGGGACCCGGACGGGGGCTAACGTTTGAG‑TGCCCCATTCACTGACATTCACAATAACATGATATTCATGACGGa                                                                        >  2:48627/1‑140 (MQ=255)
                                                                    gCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCATG‑ACCGGGGCCCGGACGGGGGGAAACGGTTGAG‑TGCCCCAGTCACGGGCCTTCACAATAAACTGTTATTGATCACggg                                                                        >  6:398632/1‑137 (MQ=25)
                                                                        cacTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGACGGGGGGAAACGTTTCAG‑TGCCCCATTCACTGCCATTCACAATAACATGATATTCATAACGGAGTGAca                                                                  >  3:418350/1‑139 (MQ=255)
                                                                        cacTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCCTCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTAAG‑TGCCACATTAACTGACATTCACAATAACATTATATTAATAACGGAGTGACt                                                                  >  1:250706/1‑140 (MQ=255)
                                                                         acTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGTCGGGGGAAAAAGTTTGAG‑TGCCCAATTAACTGACATTCCCAATAACATGATATTAATAACGGAGTGACTa                                                                 >  8:608411/1‑140 (MQ=255)
                                                                          cTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATGATC‑ACCGGGCCCCGGACGGGGGAAAACGTTTCGG‑TGCCACATGACCTGACATTCACAATAACATGATATTCATAACGGAGTGACt                                                                  >  6:466786/1‑138 (MQ=255)
                                                                           tGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCATG‑ACCGGGGCCCGGACGGGGGGAAACCTTTCAG‑TGGCCCCTTCACAGACATTCACCAAAAAAAGTTATTAATCACGGCGTGGCTa                                                                 >  6:310793/1‑138 (MQ=255)
                                                                              aTGTGCCCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTAATCATG‑ACCCGGACCCGGACGGGGGAAAACGTTTAAT‑TGGCACATTCACTGACCTTCACAAAAACATGCAATTCATAACGGATTGCAGATGTTa                                                            >  7:590385/1‑140 (MQ=255)
                                                                                   aCCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCAAAATG‑CCCCGGCCCCCGCCGGGGGGAAACGTTTAAG‑TGCCACATTAAAAGCCATTCACAAAAACAAGTTATTAATCACCGCGTGACGATGTTAAAGAt                                                       >  3:371025/1‑140 (MQ=255)
                                                                                     cTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGCATCATG‑ACCGGGACCCCGACTGGGGAAAACATTTCAG‑TGACCCATTCAAAGACATTGAAAATAACAAGAAATTAATAACCGAATTACAATGTTACAAATAg                                                     >  2:617680/1‑140 (MQ=255)
                                                                                       tCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTGATAATT‑ACCCGGACCCGGGCGGGGGGAAACGGTTCAG‑GGCCACATTAACAGCAAATGACAATAAAAATAAATTAATAAAGGAGTGTCTAAGTTACAGaaattc                                                   >  8:622091/1‑135 (MQ=255)
                                                                                       tCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTGATAATG‑ACCCGGGCCCGGCCGGGGGCAAAAGGTTCAG‑TGCCCCAGTTACGGGCATTAACAATAAAAAGATATTAATACCGGGGTGGCTATGTTTCAGAAAGtg                                                   >  8:349789/1‑139 (MQ=255)
                                                                                       tCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCATG‑ACCGGGGCCCGGACCGGGGAAAACGTTTCAG‑TTCCCCATTAACGTACATTCACAATAACATGATATTCAACACGGAGTTACTATGTTACAGATAGta                                                   >  4:467969/1‑139 (MQ=255)
                                                                                         tGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCCGGACCCGGCCGGGGGAAAACGTTTCAC‑TGGCACCATAACTGACATTAACAATAACATGATATTAATAACGGAGGGACTATGTTACAGATAGta                                                   >  5:864779/1‑137 (MQ=255)
                                                                                         tGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCATG‑ACCGGGACCCGGACGGGGGGAAACCTTTAAG‑TGCCACATTCACAGACATTCACAATAACAAGATATTAATAACGGAGTGCCTATGTTACATATAGta                                                   >  5:910776/1‑137 (MQ=255)
                                                                                             atttcttcATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGTCCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgcg                                             <  2:5129/138‑1 (MQ=255)
                                                                                              aTTCTGCATTC‑CGTCTGTGGGGCCCCGTTCTACATG‑ACCGGGCCCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgcgc                                            <  4:79618/140‑1 (MQ=255)
                                                                                                   gCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGGCACATTAAAGGACATTAACAATAACATGATATTAATGACGGAGTGACTATTTTAAAGATAGTAGGGGCGCTGAtt                                       >  3:256769/1‑140 (MQ=255)
                                                                                                   gCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGAtt                                       >  8:756521/1‑140 (MQ=255)
                                                                                                    cATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGAtta                                      >  2:600666/1‑139 (MQ=255)
                                                                                                          ccTCTCTGTGGTCCCGGGCATCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATAACGGAGTGACTATGTTACAGATAGTAGGCGCGCTGATTctgcgg                                 >  2:685137/1‑138 (MQ=255)
                                                                                                                    gTCCCGGGGATCATG‑GCCGGGCCCCCGCCGGGGGAAAACGTTTCAG‑TGGCCCATTGACTGGCATTCACAAAAACAATATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGGGGATTATGCTGATCGCAGGAt                       >  4:376548/1‑140 (MQ=255)
                                                                                                                       ccGGTCATCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCCCATTCACGGACATTCACAATAAAATGATATTCATCCCGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGAtttt                    >  4:71627/1‑139 (MQ=255)
                                                                                                                               catG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttctt            >  2:1001108/1‑140 (MQ=255)
                                                                                                                               catG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATGTGCCAttctt            >  2:363459/1‑140 (MQ=255)
                                                                                                                                 ctcccggggACCCGGACGGGGGTAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACCTGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttcttc           <  4:1005106/134‑1 (MQ=255)
                                                                                                                                 cgccgggggCCCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttct             <  5:1041006/132‑1 (MQ=255)
                                                                                                                                 aggccggggCCCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttcttc           <  4:355902/134‑1 (MQ=255)
                                                                                                                                    aCCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGcc        >  7:231516/1‑140 (MQ=255)
                                                                                                                                      ggggCCCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtt      <  8:400571/139‑1 (MQ=255)
                                                                                                                                      ggggCCCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtt      <  2:564532/139‑1 (MQ=255)
                                                                                                                                      ggggACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtt      <  7:443548/139‑1 (MQ=255)
                                                                                                                                      ggggACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtt      <  3:759005/139‑1 (MQ=255)
                                                                                                                                        ggACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtttt    >  2:950781/1‑140 (MQ=255)
                                                                                                                                        ggACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtttt    >  1:609849/1‑140 (MQ=255)
                                                                                                                                         gACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCttttt   >  8:358056/1‑139 (MQ=255)
                                                                                                                                         gACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTg   >  3:265983/1‑140 (MQ=255)
                                                                                                                                          aCCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATACCATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGCTTTGCCATTCTTCGCCTTTTGt  >  8:922009/1‑140 (MQ=255)
                                                                                                                                          aCCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTGt  >  8:3397/1‑140 (MQ=255)
                                                                                                                                          aCCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTGt  >  1:590978/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATG‑ACCGGGACCCGGACGGGGGAAAACGTTTCAG‑TGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTTGT  >  NZ_CP022686/4677911‑4678186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A