Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F2 I0 R1 233 6461.8 9239822 98.3% 9082745 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,678,047 A→C 21.6% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,678,0470AC21.6% 75.2 / 2.4 100intergenic (+45/‑76)agn43/CIW80_RS23450autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (54/14);  new base C (16/2);  total (84/16)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

CCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTT  >  NZ_CP022686/4677911‑4678184
                                                                                                                                          |                                                                                                                                           
cccTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑A‑ccc                                                                                                                                         >  8:184499/1‑140 (MQ=255)
cccTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCCTCATGACCGGG‑A‑cgc                                                                                                                                         >  3:346582/1‑138 (MQ=255)
   tGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGAGCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑T‑CCCggc                                                                                                                                      >  2:757593/1‑139 (MQ=255)
     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTTAATGAGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCAGAATGACCGGG‑G‑CCCGGCCg                                                                                                                                    >  2:247131/1‑140 (MQ=21)
     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCATGCCCGGG‑A‑CCCGGACg                                                                                                                                    >  7:617829/1‑140 (MQ=255)
     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGCCg                                                                                                                                    >  2:495826/1‑140 (MQ=255)
     ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATAATGACCGGG‑A‑CCCGGGCg                                                                                                                                    >  8:142557/1‑140 (MQ=255)
     ggCGTTCAGGCCGGTTATGCCAACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACGTAATGTGCCCTTCTGATAATTCTTCATTGCCTCTCTGTGTT‑CCCGGTAATAATGACCGGG‑T‑CCCGGACg                                                                                                                                    >  8:345982/1‑140 (MQ=21)
       cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑ccccgcag                                                                                                                                    >  5:986929/1‑133 (MQ=255)
       cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGACTAATGCCCGGG‑A‑CCCGGACg                                                                                                                                    >  5:6603/1‑138 (MQ=21)
       cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCGTCATGACCCGG‑c‑ccccgcac                                                                                                                                    >  5:6601/1‑133 (MQ=21)
                     aTGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCTCATGACCGGG‑G‑CCCGGACGGGGGAAAACGttta                                                                                                                      >  6:685854/1‑137 (MQ=255)
                        cccACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGGCCGGG‑A‑CCCGGGCGGGGGAAAACGGTTAAGTgg                                                                                                                 >  1:650065/1‑139 (MQ=37)
                        cccACACCATCACCGGCAGCTGCGTTTATGGTTATACCAGCCCGGCCACATTCATTGTCAACTTCTTATATTTCTCCATGCCCCGTCTGGGGC‑CCCGGCCATCACCCCGGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGt                                                                                                                   <  6:638897/138‑1 (MQ=255)
                            caGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCAAGACCCGG‑A‑CCCGGACCGGGGAAAACGATTCCGTGCcacc                                                                                                             >  7:703362/1‑139 (MQ=37)
                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATACTTAAAGGG‑T‑CCCGGACGGTGGTTAACGGTTAAGttcagcat                                                                                                            >  3:47895/1‑133 (MQ=21)
                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGCCCGGG‑C‑CCCGGGCGGGGGGAAACGGGTCAGTGCCCCAt                                                                                                            >  7:1009873/1‑140 (MQ=37)
                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACAt                                                                                                            >  4:52859/1‑140 (MQ=255)
                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACAt                                                                                                            >  4:1062250/1‑140 (MQ=255)
                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCCGG‑C‑CCCGGACGGGGGAAAACGTTTAAGTGGCCCAt                                                                                                            >  3:859204/1‑140 (MQ=37)
                             aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATAATGACCGGG‑A‑CCCGGCCCGGGGGAAACCTTTAAGTggcccaa                                                                                                            >  4:200779/1‑134 (MQ=25)
                                  cAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CTCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACt                                                                                                       >  2:1087484/1‑140 (MQ=255)
                                  cAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTAAcc                                                                                                       >  2:946881/1‑139 (MQ=255)
                                  cAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCCGACGGGGGAAAACGTTTAAGTGCCACATTAACt                                                                                                       >  2:495959/1‑140 (MQ=255)
                                       gcagcaGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAAATCTGCATTGCCTCTCTGTGGT‑CCCCGTGATAATTACCGGG‑A‑CCCGCAAGGGGGACAACCAATCACTGCCACCTTAACAGaa                                                                                                    >  5:828508/1‑137 (MQ=21)
                                         agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACCTTTCAGTGCCACCTTACCAGACAtta                                                                                                >  2:1154602/1‑139 (MQ=37)
                                         agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑C‑CCCGGCCGGGGGAAAACGTTTAAGTGCCACAATCACTGACaata                                                                                                >  2:204210/1‑137 (MQ=37)
                                         agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGCTCATGACCCGG‑A‑CCCGGACGGGGGGAAACGTTTCAGTGCCCCATTCAATGACaata                                                                                                >  4:3826/1‑137 (MQ=37)
                                         agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATAATTCCCGGG‑A‑CCCGGACGGGGGAAAACGTTTAAGTGCCACATTCACTGACaa                                                                                                  >  5:266029/1‑137 (MQ=37)
                                          gctgcgcTGATGTTTATAACAGTCCGGACACACTTATTGTCACCTTCTTATAATTCTGAATTCCCTCTGTGTGGG‑CCCGGCCATCATGCCGGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTca                                                                                               <  4:419906/137‑1 (MQ=255)
                                          gcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGGATCAAGACCGGG‑A‑CACGGACGGGGGAAAAACTTTCAGGGCCACAATAACTGACATTca                                                                                               >  1:856831/1‑140 (MQ=37)
                                           cagcGCTGAAGGTTATAACAGTCAGGCCACACTGCATGTGACCGTCTGATAATTCTGCATTTCCTCTCTGTGGT‑CCCGGTCATGAAGCCCGGG‑C‑CCCGGCCGGGGGACAACGTGTCAGTGACCCATTCACTGAccatc                                                                                                >  6:1062095/1‑134 (MQ=25)
                                           cagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGGAACATGACCGGG‑A‑CCCGGACGGGGGAAAAAGGTTCAGTTCCACCTTCAGAGACATTc                                                                                                >  5:661366/1‑138 (MQ=37)
                                            agcTCTGAAGGGTAACACAGTCCGGCCACACTGAATGTGAACTTCAGATATTTCTTCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGTG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTcaca                                                                                             <  7:680123/140‑1 (MQ=255)
                                               gcTGATGTTTAAAACAGCCCGCCCACACTGATTGGTACCTTCTTATAATTCTGATTTGCCTCTCTGTGGC‑CCCGGCCATCATGCCGGGT‑C‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATa                                                                                         <  2:529872/140‑1 (MQ=255)
                                                cTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑C‑CCCCGGCGGGGGGAAACGTTTAAGTGGCACATTCACTGACATTAACa‑aa                                                                                          >  5:1082259/1‑137 (MQ=255)
                                                    cggTTATAACAGTTATCCCACTCTCATTTTAACTTTCTTTTAATTTTGCATTGCCACTTTTGGGC‑CCGGGAAATCAGCACGGGC‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATg                                                                                    <  4:667783/139‑1 (MQ=255)
                                                        tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑G‑CCCGGCCGGGGGCAAACGGTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGatat                                                                                >  2:1167806/1‑140 (MQ=255)
                                                        tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑A‑CCCGGGCGGGGGAAAACGTTTCAGTGCCACAATGACTGACATTCACA‑AAAACATGaaat                                                                                >  4:543671/1‑140 (MQ=38)
                                                        tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CACGGTCAAGATGACCCGG‑A‑CCCCGACGGGGGAAAAAGTTTCAGTGCCACAATAACGGACATTCACA‑ATAACAttaaat                                                                                >  8:452188/1‑135 (MQ=255)
                                                        tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCAGTGCCTCTCTGTGGT‑CCCGGGCATCCAGACCGGG‑A‑CCCGGCCGGGGGCAAACGGTTACGTGCCACATTCAATGACAGTAACA‑ATAAAATGatat                                                                                >  4:828930/1‑140 (MQ=37)
                                                         ataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCTCATGACCGGG‑A‑CCCGGACGGGGGGAAACGGTTCAGTGACCCATTCACAGACATTCACA‑ATAACATGATAtt                                                                               >  2:272674/1‑140 (MQ=255)
                                                          taACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCCTGCCCGGG‑G‑CCCGGGCGGGGGGGAAAGTTTAAGTGCCCCATTCACTGACCTTAACA‑ATAAAATTatat                                                                                >  6:514573/1‑138 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATCATTGACGGG‑C‑CACGGACGGGGGAAAACGTTTCAGTGACCCATTCACTGAAAATCACA‑ATAAAATTATATTCATCACg                                                                        >  7:839680/1‑140 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGCCCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACg                                                                        >  2:917338/1‑140 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑C‑CCCGGTCGGGGGAAAACGTTTCAGTGCCCCATTCACTGTCATTCACA‑ATAACATGATTTTCATAACg                                                                        >  7:183471/1‑140 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTACTCATGACCGGG‑G‑CCCGGGCGGGGGAAAACGGTTAAGAGCCACCTTCACGGTCCTTCACA‑ATAAAAAGATATTCATCACg                                                                        >  7:352890/1‑140 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATGATGCCCGGG‑G‑CCCCGCCGGGGGGAAAACTTTAAGTGCCCCAGTCACTGACCTT‑ACACATAAAATGATATTAATAAcc                                                                        >  1:1007929/1‑139 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGATCATGACCGGG‑A‑CCCCGCCGGGGGGAAACGTTTAAGTGGCCCATTCACTGCCAATCAAA‑AAAAAATGATATTAATCAcc                                                                        >  1:822855/1‑139 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑A‑CCCGGTCGGGGGTAAACGTTTGAGTGCCACATTAACAGACATTCACA‑ATAACATTATTTTAATAACg                                                                        >  3:924774/1‑140 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATGACCGGG‑C‑CCCGGCCGGGGGGAAACCTTTCAGTGGCCCAATAACAGACATTAACA‑AAAACAAGATATTCATAAcc                                                                        >  3:530078/1‑139 (MQ=255)
                                                                tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGACTCTCTGTGGT‑CCCGGGGATCATTACCCGG‑C‑CCCGGGCGGGGGAAAACGGTTCAGGGCCCCATTCACTGAGCTTCACC‑AAAACATTATATTCATCACg                                                                        >  7:184373/1‑140 (MQ=255)
                                                                gccccccACCCTTAAGGTGACATTCTTATAATACTCACTCGCCGCTCGGGGGC‑CCGGCTCAACA‑GCCCGGG‑CCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATtcatca                                                                          <  6:444051/133‑1 (MQ=255)
                                                                  cggCCACACTTATGGGCACCTTATTATAATTCGGAAGGGCCGCCCTGGGGC‑CCGGGCCATCATGCCCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGa                                                                      <  2:1127722/139‑1 (MQ=255)
                                                                  aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCTGATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACGGACATTCACA‑ATAACAAGATATTCATGACg                                                                        >  6:1027439/1‑138 (MQ=255)
                                                                  aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑A‑CCCGGCAGGGGGCCAAAGTTTCAGTGCCACATTGCATGACATTCACA‑ATACAATGGTATtcaaaccg                                                                        >  6:1030561/1‑133 (MQ=255)
                                                                    gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTGCTGATGGACGGG‑T‑ACCGGTCGGGGGAAAACGTTTCAGTGGCCCCATAACTGTCAGTGACA‑ATAACAAGATATTCATAAccgt                                                                      >  6:924372/1‑135 (MQ=255)
                                                                     ccACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCCTCATTACCCGG‑G‑CCCGGGCGGGGGAGAACGGTTCAGTGCCACCTTAACTGACAATAGCA‑ATAACAAGATATTCAACACGGAggg                                                                   >  4:526402/1‑138 (MQ=255)
                                                                        cacTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCTACATGAACGGG‑A‑ACCGGACGGGGGGAAACGTTTAATTGGCACATTAACTGACATTCACA‑AAAACATGATATTAATAACGGAGTGAcg                                                                >  1:149790/1‑139 (MQ=255)
                                                                        cacTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATGACCGGG‑G‑CCCCGCCGGGGGAAAACGTTTAAGTGCCCCCTTCACTGACATTAACA‑ATAAAAAGATATTAATAACGGAGTGACt                                                                >  2:1174955/1‑140 (MQ=255)
                                                                        cacTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATGACCCGG‑A‑CCCGGACGGGGGCAAACGGTTCAGTGCCACATTCACTGACCATAAAA‑ACAACCTGATATTCCTCACGGAGTGAcg                                                                >  2:310314/1‑139 (MQ=255)
                                                                        attttattgtcACTTTCTGATATTTGTCTATTCCCTCTCTGTGGG‑CCGGGACAACTTTCCTGTG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACt                                                                <  3:1060643/133‑1 (MQ=255)
                                                                          cTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTAAACATGACCGGG‑A‑CCCGGAGCGGGGAAAAAGTTTAAGTTCCAAATTAAATTAAATTCACA‑ATAAAAAGAAATTAAAAAAGGAGTGACt                                                                >  6:863699/1‑138 (MQ=255)
                                                                              aTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGGCAACA‑GACCGGC‑C‑CCCCGGCGGGGGGAAACGTTTAACGGGCACCATCACGGGCATTACCA‑AAAAAAAGATATTAATAACCGGGTGAGTATGttt                                                          >  7:201148/1‑139 (MQ=255)
                                                                              aTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCAAAATTACCGGG‑A‑CCCGGCCGGGGGCAAACGTTTCAGTGCCACATTCACAGAACTTCACA‑AATACATTATATTAAAAACGGAGTTAcgtagtga                                                          >  2:144876/1‑133 (MQ=255)
                                                                              aTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATAATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACCTTCACTGACATTCACA‑ATAACAATATATTCATCAACGAGTGACTATGTTa                                                          >  7:361668/1‑140 (MQ=255)
                                                                                  gACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCAACATGACCGGG‑C‑CCCGGACGGGGGAAAACGTTTAAGTGACACAATAAAGGACATTCACA‑ATAACATGAAATTAATCACGGAGGGACTATTGTACAGa                                                      >  3:1039809/1‑140 (MQ=255)
                                                                                   aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGGAAACGTTTCAGTGCCACATTAACTGACATTCACA‑ATAACATGATATTCATCAAGGAGTTACTATGTTAAAGAt                                                     >  2:281177/1‑140 (MQ=255)
                                                                                   aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGttt                                                                                                                       <  4:58127/75‑1 (MQ=255)
                                                                                   aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGttt                                                                                                                       >  3:58127/1‑75 (MQ=255)
                                                                                   aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACCTTAACTGACATTCACA‑ATAACATGATATTAATCACGGAGTGACTATGTTACAGAt                                                     >  7:62403/1‑140 (MQ=255)
                                                                                   aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACAATAACTGACATTCAAA‑ATAACATTATATTCAACACGGAGTTAATATGTTACAGAt                                                     >  7:62408/1‑140 (MQ=255)
                                                                                   aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCATCATGACCGGG‑C‑CCCCGCCGGGGGAAAACCTTTCCGTGCCCCATTAACTGCCATTCACA‑ATAAAAAGAATTTAATACCGGAGTGACTATGTTACACAt                                                     >  2:443551/1‑140 (MQ=255)
                                                                                     cTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTAATCATGAACGGG‑A‑CCCGGACGGGGGAAAACGTTTAAGTGCCACATTAACTTACATTCACA‑AAAACATGATATTAATCACGGAGTGACTATTTTACAGAt                                                     >  6:772581/1‑138 (MQ=255)
                                                                                     cTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGCACAATGAACCGG‑G‑CCCCGCCGGGGGCAAACGATTAAAGGCCCCATTCACTGACATTCCAA‑ATAACATGATATTAATCACCGAGTTGATAGTTTACAGAt                                                     >  5:496403/1‑138 (MQ=255)
                                                                                       tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGGATCATGACCGGG‑C‑CCCGGCCGGGGGAAAAAGTTTACTTGCCACAATCCCGGACATTCACC‑ATAAAATTAAATTCATGACGGATTGACTATTTTAAAGATATTc                                                 >  8:83165/1‑140 (MQ=255)
                                                                                       tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGACCGGG‑A‑CCCGGCCGGGGGTAAACGTTTCAGTGCCCCATTCAATGACATTGACA‑ATAACATGATATTCATCACGGAGTGACGATGTTACAGATAGtg                                                 >  2:1107336/1‑139 (MQ=255)
                                                                                       tCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑C‑CCCCGGCGGGGGGAAACGTTTCAGTGCCCAAATAACAGCCATTCAAC‑ATAACATGATATTCATGACGGAGTGACAATGTTACAGATAGTc                                                 >  3:1102525/1‑140 (MQ=255)
                                                                                       tCTGATAATTCTGCATTGCCTCTAACTGGT‑CCCGAACGTCAAGCCACGG‑A‑AACGGCCGGGGGAAAAAGTTTCAGTCCCACATAAACACACAAAAACA‑ATAACATCATATTAATGACGGAGTTACAATATTTAAGATAgaa                                                 >  4:974918/1‑138 (MQ=255)
                                                                                         tGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTGATAATTACCCGG‑A‑CCCGGTCGGGGGAAAACGTTTCACTGCCACATTCACTGACATTGACA‑ATAAAATGATATTAATCACTTTGTGACTATGTTAAAtatattt                                                 >  6:683376/1‑135 (MQ=255)
                                                                                              aTTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGCCGGGGGAAAACGGTTCAGTGCCACATTCACTGACATTCACC‑ATAACATGATATTCATAACGGAGTGACTATGTTACAGATAGTCGgcgcgc                                          >  4:875134/1‑140 (MQ=255)
                                                                                               ttCTGCATTGCCTCTCTGTGGT‑CCCCGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTAAGTGCCACATTAACAGACATTAACA‑ATAAAAAGATAATAAAAACGGAGTGACTATTTTAAAGATATTAGgcgcgca                                         >  2:1118143/1‑139 (MQ=255)
                                                                                                  tGCATTGCCTCTCTGTGGT‑CCCGGTCATCATTACCGGG‑A‑CCCCGGCGGGGGAAAACGTTTAAGTGGCACCTTCACTGACATTCACA‑ATAAAATTATATTAATAACGGAGGGACTATGTTAAAGATAGTCGGCGCGCTGAt                                      >  1:742153/1‑140 (MQ=255)
                                                                                                  tGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑ACCGGGCGGGGGAAAACGTTTCAGTGCCACATTCACGGACATTAACA‑ATAACATGATTTTCATAACGGAGTGACTATGTTACAGATAGTCGGCGGGCTGAt                                      >  4:115276/1‑140 (MQ=255)
                                                                                                  tGCATTGCCTCTCTGTGGT‑CCCGGGCATCATGTCCGGG‑A‑CCCGGCCGGGGGGAAACGTTTGAGTGCCACATTCACTGACATTCACA‑ATAACATGAATTTCCTCACGGAGTGAGTATGTTACAGCTATTCGGGCCGCTGAt                                      >  4:276731/1‑140 (MQ=255)
                                                                                                              ctGTGGT‑CCCCGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGGTTAAGTGCCCCAATCACAGACAATCACA‑ATAACAATATATTCATCACGGAGTGACTATGTTTCAGATAGTCGGGGCGCGGATTATGGTGATCgg                          >  2:59698/1‑139 (MQ=255)
                                                                                                                gtgGT‑CCCGGTCATCATGACCGGG‑G‑CCCGGACGGGGGAAAACGTTTAAGTGGCACATTCACTGACATTCACA‑AAAACATGATATTAATCACGGAGTGACGTTGTGACAGATAGTCGGCGCGCGGATTCTGCGTATCGc                          >  6:80508/1‑138 (MQ=255)
                                                                                                                 gggT‑CCGGGCCATCA‑GCCCGGGCA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCa                         <  5:639837/137‑1 (MQ=255)
                                                                                                                   gT‑CCCCGTCCTCATGACCGGG‑C‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAATCACA‑ATAACATGATAATAATCACGGAGTGGCTATGTTACAGATAGTTGGCGCGCTGATTATGCAGTTCGCAGGAt                     >  8:136933/1‑140 (MQ=255)
                                                                                                                    t‑CCCGGGCCTAATGACCGGG‑C‑CCCGGCCGGGGGGAAACGTTTCAGTGCCACATTCACAGACATTAACC‑AATACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCGGCTTAAGCGGGTCGCAGGGtt                    >  4:312221/1‑140 (MQ=255)
                                                                                                                      ccccGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTAAGTGCCACATTGACTGACATTAACA‑ATAACATGATATTCATCACGGAGTTAATATGTTAAAGATAGTAGGCGCGCTGATTATGCAGATCGCAGGAt                     >  6:691698/1‑138 (MQ=255)
                                                                                                                        gggTCATCAGCACGGGG‑A‑CCCGGACGGGGGAAATCGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGc                 <  8:535147/139‑1 (MQ=255)
                                                                                                                               catGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGAGGATCGCAGGCTTTGCCAttctt          >  8:808281/1‑140 (MQ=255)
                                                                                                                                tgccccGGT‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGTTTCTGCTGATCGCATGATTTGCCAttcttc         <  4:1080468/136‑1 (MQ=255)
                                                                                                                                cggcccGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttcttc         <  4:689845/136‑1 (MQ=255)
                                                                                                                                atcccgggg‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttcttc         <  8:270782/134‑1 (MQ=255)
                                                                                                                                 tGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCg        >  1:475665/1‑140 (MQ=255)
                                                                                                                                 tGACCGGG‑A‑CCCGGACGGGGGAAAACGGTTCAGGGCCCCAATCACTGACATTCACA‑AAAACATGATATTCAGCCCCGAGTGACTATGTTACAGATAGTCGGCGCGCTGAATCTGCTGATCGCAGGATTTGCCTtactt          >  5:47270/1‑138 (MQ=255)
                                                                                                                                      gggca‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtt    <  4:197104/136‑1 (MQ=255)
                                                                                                                                       gg‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCtttt  >  7:529465/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTCATCATGACCGGG‑A‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACA‑ATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTT  >  NZ_CP022686/4677911‑4678184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A