Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F13 I0 R1	150	9496.0	7621532	98.4%	7499587	136.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	1,360,750	G→T	10.2%	intergenic (‑85/+239)	kgtP ← / ← rrf	alpha‑ketoglutarate permease/5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	1,360,750	0	G	T	10.2%	417.7 / 8.2	178	intergenic (‑85/+239)	kgtP/rrf	alpha‑ketoglutarate permease/5S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base G (53/101); new base T (16/8); total (69/109)
Fisher's exact test for biased strand distribution p-value = 3.40e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

ACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTGAATCTGGCTT  >  NZ_CP022686/1360612‑1360889

 cgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCa                                                                                                                                           <  3:257226/140‑1 (MQ=255)

 cgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCa                                                                                                                                           <  2:959564/140‑1 (MQ=255)

 cgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCa                                                                                                                                           <  2:74165/140‑1 (MQ=255)

 cgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCa                                                                                                                                           <  7:313293/140‑1 (MQ=255)

 cgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCa                                                                                                                                           <  2:113259/140‑1 (MQ=255)

 cgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCa                                                                                                                                           <  1:600254/140‑1 (MQ=255)

 cgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGGTACAGTACTTTCCGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCa                                                                                                                                           <  1:865563/140‑1 (MQ=255)

 cgaGTATCACTACTTGGCAGTTTGCTGGCTGCAGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATACTATGCTTTTGTCTGTCGCGTTTGTTTATTTTTTTGTAAGAAA‑AATGTTATACATTTGTTGCa                                                                                                                                           <  5:565309/140‑1 (MQ=255)

    gTATCACTACTTGTCAGTTTGCTGTCTGCCGGTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCatat                                                                                                                                        <  4:772856/140‑1 (MQ=255)

      agcACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCAGCCGTAATCCAATGCTTTTGTCGGGCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCa                                                                                                                                      <  5:167260/138‑1 (MQ=255)

      aTCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCa                                                                                                                                      <  2:732588/140‑1 (MQ=255)

            actTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGc                                                                                                                                <  7:892075/140‑1 (MQ=255)

               tGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTTTTTATTTTTTTTTAAGAAA‑AATGTTATACATTTTTTGTATATAATTAttaaaa                                                                                                                             >  5:347215/1‑135 (MQ=255)

                 tCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCt                                                                                                                           <  5:458023/140‑1 (MQ=255)

                 tCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCAGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCt                                                                                                                           <  5:19914/140‑1 (MQ=255)

                 acAGATTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCt                                                                                                                           <  8:665775/139‑1 (MQ=255)

                     tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAATAAA‑AATGTTATACATTTTTTGCATATAATTATGCAACCATacc                                                                                                                       >  2:411340/1‑138 (MQ=255)

                     tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAAc                                                                                                                       <  3:324267/140‑1 (MQ=255)

                     tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAAc                                                                                                                       >  3:764641/1‑140 (MQ=255)

                     tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAAc                                                                                                                       <  4:577009/140‑1 (MQ=255)

                     tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AAAGTTATACATTTGTTTCATATCATTATTCAACCTTAAc                                                                                                                       >  8:452183/1‑140 (MQ=255)

                     tttGCTGTCTGCCGTTACAGTACTTTCAACCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTTTTGCATATAATTATGCAACCTTAAc                                                                                                                       >  4:617186/1‑140 (MQ=255)

                       tGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCa                                                                                                                     <  5:611627/140‑1 (MQ=255)

                       tGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGGCGCTTTTTTTTATTTTTTGGTAATAAA‑AATGTTTTTAATTTGTTGCATATAATTATGCAACCTTAACCa                                                                                                                     >  8:298542/1‑140 (MQ=255)

                       tGCTGTCTGCAGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATACAATGCTTTTGTCGTTCGCTGTTTGTTATTTTTTAGTAATAAA‑AAAGTTATAAATTTTTTGAAAATCATTATTCAACCATAAcaa                                                                                                                     >  4:385009/1‑138 (MQ=255)

                          tGTCTGCCGTTTCAGTACTTTCATCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATTTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGa                                                                                                                  <  3:968115/140‑1 (MQ=255)

                                   ttACAGTACTTTCAGCCATGCCATTATGTCTCCTGCTGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTTTTGCATATCATTATGCAACCTTAACCATGAATttagtta                                                                                                         >  1:542073/1‑140 (MQ=255)

                                           cTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTTTTGCATATAATTATGCAACCTTACCCATGAATTTAGTTAGCAgtgaa                                                                                                 >  4:27413/1‑136 (MQ=255)

                                             ttCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAAt                                                                                               <  4:262021/140‑1 (MQ=255)

                                              tCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTGTTATAAA‑AATATTTTACATTTTTTTCATATCATTATGAAACCTTAACAATGAATTTAGTTTGGAGGAAAAtt                                                                                              >  7:586414/1‑139 (MQ=255)

                                              tCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCGTTTGTTTTTTTTTTGGTAAGAAA‑AATTTTATACATTTTTTGCATTTAATTATGAAACCTTAACCATGAATTTATTTAGCAGGAAAAtt                                                                                              >  3:266623/1‑139 (MQ=255)

                                                aGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTGTAAGAAA‑AATGTTATACATTTTTTGAATATCATTTTGCAACCTTAAACATGAATTTATTTTGCAGGAAAATggg                                                                                            >  3:865306/1‑139 (MQ=255)

                                                   catgccatTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTGTTAATAAA‑AATTTTAAACAATTTTTGCATATCATTATGCAACCTTAAACATGAATTTTGTTATCAGGAAAATTGtttt                                                                                         >  1:438326/1‑138 (MQ=255)

                                                     tgccatTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTTTTTTTTTTTTGGTTATAAA‑AATGTTATAAATTTTTTGCATATAATTATTCAACCTTAACCATGAATTTAGTTATCAGGAAAATGGttatta                                                                                       >  3:650016/1‑140 (MQ=255)

                                                     tgccatTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATg                                                                                                                                 <  6:752523/98‑1 (MQ=255)

                                                     tgccatTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATg                                                                                                                                 >  5:752523/1‑98 (MQ=255)

                                                     tgccatTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATAAATTTGTTGAATATCATTATGAAACATTAACCATGAATTTATTTAGCAGGAAAATTGttattt                                                                                       >  5:328726/1‑139 (MQ=255)

                                                     tgccatTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTGTAAGAAA‑AATTTTATACATTTTTTGCATTTAATTTTGGAACCTTTACCATGAATTTAGTTAGCAGGAAAATGGtttttt                                                                                       >  1:257552/1‑139 (MQ=255)

                                                     tgccatTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTGTAAGAAA‑AAAGTTATACAATTTTTTCATATAATTATGCAACCTTAACCATGAATTTTGTTATCAGGAAAATGGttttta                                                                                       >  7:335250/1‑140 (MQ=255)

                                                     tgccatTATGTCTCCTGCCGTAATCCAATGCGTTTGTCGGTCGCTTTTTTTTATTTTTTGGTAAGAAATAATTTTTTA‑ATTTTTTGATTATCATTATGCAACAGTAACCAGGAATTTATTTAGAGTGAAAATGGtttttt                                                                                       >  4:407448/1‑139 (MQ=255)

                                                      gccatTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATTCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGttattat                                                                                      >  1:65315/1‑139 (MQ=255)

                                                      gccatTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTGTTTTTTTTTTTTTGGTTAAAAA‑AATTTTATACAATTGTTTCATATAAATATGAAAACATAACCATTAAATTATTTAGCAGGAAAATTGGTATTaa                                                                                      >  3:493770/1‑140 (MQ=255)

                                                        catTATGTCTACTGACGTAATCCTATTCTTTTGTCGGTATCTTTGTTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCGTATCATTATGAAACCTTAAGGATGAATTTAGTTAGCATTAAAATGGTTATTAAgg                                                                                    <  4:860834/140‑1 (MQ=255)

                                                               tctcCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAATAAA‑AAAGTTATACATTTTTTGCATATCATTATTCAACCTTAACCATTAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCATaa                                                                             >  8:377280/1‑140 (MQ=255)

                                                                ctcCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAg                                                                            >  2:445550/1‑140 (MQ=255)

                                                                  ccTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTTTTTATTTTTTGGTAATAAA‑AATTTTATAAATTTTTTTCATATAATTATTCAACCTTAACCATTAATTTAGTTATCAGGAAAATTGTTATTAAAGAGCTTAATGa                                                                          >  1:2976/1‑140 (MQ=255)

                                                                  ccTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGa                                                                          <  5:706194/140‑1 (MQ=255)

                                                                  ccTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGa                                                                          <  1:339395/140‑1 (MQ=255)

                                                                   cTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTTAGAAA‑AATGTTTTAAATTTTTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTTAGGAGGTTAAGGAt                                                                         >  6:112235/1‑140 (MQ=255)

                                                                         tAATCCAATGCGTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGAttt                                                                   <  6:254003/140‑1 (MQ=255)

                                                                          aaTCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTc                                                                  <  8:318873/140‑1 (MQ=255)

                                                                           aTCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTGTTAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAATGAGCTTAAGGATAGATTTCt                                                                 >  3:787189/1‑140 (MQ=255)

                                                                           aTCCAATGCTTTTGTCGGTCGCGTTTTTTTTTTTTTTGGTTAGAAA‑AATGTTATTAATTTTTTTGAGATGATTATTGAACCGTAACAATGAAATTAGTTTGCAGGAAAATGGTTATTAATTAGCTTAAGGATTGATTTcg                                                                 >  5:38093/1‑139 (MQ=255)

                                                                             ccAATGCTTTTGTCGGTCGCGTTTGTTTTTTTTTTGGTAAAAAA‑AAATTTATAAAATTTTTGCATATAATTATTCAACCTTAAACAATAATTTTTTTAGAAAGAAAAATGTTATTAAAGAGCGTAAGGATATATTtgtgt                                                               >  6:513041/1‑139 (MQ=255)

                                                                              cAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTATGGAGCTTAAGGATAGATTTCTGGt                                                              <  2:65315/140‑1 (MQ=255)

                                                                              cAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGt                                                              <  1:172607/140‑1 (MQ=255)

                                                                               aaTGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTa                                                             <  4:355704/140‑1 (MQ=255)

                                                                               aaTGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTa                                                             <  4:14553/140‑1 (MQ=255)

                                                                                 tGCTTTTGTCGGTCGCTTTTTTTTATTTTTTGGTAAAAAA‑AAATTTATAAAATTTTTGCATATAATTATGACACCTTAACCAAGAATTTATTTAGCATGAAAATAGTTATTAAGATGCGTAAATATAGAATTATGGTaat                                                           >  7:116623/1‑139 (MQ=255)

                                                                                 tGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAag                                                           >  2:183211/1‑140 (MQ=255)

                                                                                 tGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAag                                                           >  2:608309/1‑140 (MQ=255)

                                                                                  gCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAagg                                                          <  1:126955/140‑1 (MQ=255)

                                                                                  gCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAagg                                                          <  6:758758/140‑1 (MQ=255)

                                                                                     tttGTCGGTCGCTTTTGTTTATTTTTTGGTAAGATA‑AATGTTCTAAATGTGTTGCTTATACTTATGCATCCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTACGGAGCTTAAGTATAGATTTCTGGTAaggagg                                                       <  4:693501/140‑1 (MQ=255)

                                                                                     tttGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑ATTGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATATAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAaggagg                                                       <  6:172757/140‑1 (MQ=255)

                                                                                     tttGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACGTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAaggagg                                                       <  5:257952/140‑1 (MQ=255)

                                                                                     tttGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAaggagg                                                       <  7:276925/140‑1 (MQ=255)

                                                                                     tttGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAaggagg                                                       <  6:347215/140‑1 (MQ=255)

                                                                                     tttGTCGGTCGCTTTTGTTTATTTTTTGGGAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGACTTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAaggagg                                                       <  3:408210/140‑1 (MQ=255)

                                                                                     tttGTCGGGCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAaggagt                                                       <  3:412637/140‑2 (MQ=255)

                                                                                      ttGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTTTTTCATATAATTATGCAACCTTAACCATGAATTTAGTTATCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAggagga                                                      >  4:805476/1‑140 (MQ=255)

                                                                                      ttGTCGGTCGCGTTTTTTTATTTTTTTGTAAGAAA‑AATGTTATAAATTTTTTTCATATCATTATGCAACCATAACCAAGAAATTATTTAGCAGGAAAATGGTTATTAATGACCTTAAGGATATATTTCTTGTAAtgagga                                                      >  2:646084/1‑140 (MQ=255)

                                                                                             tcgCGTTTGTTTATTTTTTTGTAAGAAA‑AAAGTTATACAATTGTTGCATATCATTAAGCAACCTTAACAAAGAATTTAAATAGCAGGAAAATGGTTATTAAAGAGCTTAAAGATATATTACTTGTAAAGAGTAAGTATgg                                               >  6:720463/1‑140 (MQ=255)

                                                                                                    tGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTggg                                        <  2:136249/140‑1 (MQ=255)

                                                                                                    tGTTTATTTTTTGGTAAGAAA‑AATGGTATACATTTGTTGCATATGATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTggg                                        <  6:593152/140‑1 (MQ=255)

                                                                                                         aTTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAAtt                                   <  4:764641/140‑1 (MQ=255)

                                                                                                             tttGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTggg                               >  8:517618/1‑140 (MQ=255)

                                                                                                              ttGGTAAGAAA‑AATGTTATACATTTGTCGCCTATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATTGAAGTGGGCAATTTgggg                              <  1:744737/140‑1 (MQ=255)

                                                                                                                ggTAAGAAA‑AATGTTATACATTTGTTTCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGaa                            <  2:450483/140‑1 (MQ=255)

                                                                                                                ggTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATTCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGaa                            <  1:773604/140‑1 (MQ=255)

                                                                                                                ggTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGaa                            <  4:780585/140‑1 (MQ=255)

                                                                                                                ggTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGaa                            <  3:721692/140‑1 (MQ=255)

                                                                                                                ggTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGaa                            <  3:441738/140‑1 (MQ=255)

                                                                                                                ggTAAGAAA‑AATGGTATACATTTGGTGCATATCATTCTGCAACCTTAACCAAGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGaa                            <  2:834971/140‑1 (MQ=255)

                                                                                                                     taaa‑aaTGTTATACATTTGTTGCATATCATTATGCAACCTTAAACATGAATTTAGTTATCATGAAAATGGTTTTTAAGGAGCTTAAGTATAGATTTCTGGTAAGGAGTAAGTATGGTAGTTGGCAATTTGGTGaataggt                       <  2:30671/139‑6 (MQ=255)

                                                                                                                        a‑aaTGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTAtt                    <  6:761071/140‑1 (MQ=255)

                                                                                                                        a‑aaTGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTAtt                    <  7:712749/140‑1 (MQ=255)

                                                                                                                           aTGTTATACATTTGTTTCATATCCTTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGAGAGATTTATGGTCAGGAGGAAGTATGGAATTGGGCAATTTGGGGAAGACGTATTCg                  <  5:489248/140‑1 (MQ=255)

                                                                                                                                 taCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTg            >  3:953151/1‑140 (MQ=255)

                                                                                                                                 taCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTg            >  6:921947/1‑140 (MQ=255)

                                                                                                                                    aTTTGTTGCATATCATTCTGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGCTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTGAAt         <  6:444882/140‑1 (MQ=255)

                                                                                                                                    aTTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTGAAt         <  4:460406/140‑1 (MQ=255)

                                                                                                                                    aTTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTGAAt         <  2:169009/140‑1 (MQ=255)

                                                                                                                                      ttgttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTGAATCt       >  2:909654/1‑140 (MQ=255)

                                                                                                                                      ttgttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTGAATCt       >  3:926179/1‑140 (MQ=255)

                                                                                                                                      ttgttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTGAATCt       >  6:167090/1‑140 (MQ=255)

                                                                                                                                       tgttgCATATGAATATTCATCCTTAAACATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGCGGGCAATTTGGGGAAGACGTATTCGCTGTTGAATCTg      <  7:771898/140‑1 (MQ=255)

                                                                                                                                        gttgCATATCATTATGCAACCTTAACCAGGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTGAATCTgg     >  7:739449/1‑140 (MQ=255)

                                                                                                                                         ttgCATATCATTATGCAACCTTAACCATTAATTTACTTAGCAGTACACTGGTTTTTAATGAGGTTAAGGATAGATTTCAGGTAAGGAGGAATTCTGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTGAATCTGGc    <  2:823657/140‑1 (MQ=255)

                                                                                                                                           gCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTGAATCTGGCtt  >  7:634392/1‑140 (MQ=255)

ACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCAATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGGTAAGAAA‑AATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTAAGGAGCTTAAGGATAGATTTCTGGTAAGGAGGAAGTATGGAAGTGGGCAATTTGGGGAAGACGTATTCGCTGTTGAATCTGGCTT  >  NZ_CP022686/1360612‑1360889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A