Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F13 I0 R1 150 9496.0 7621532 98.4% 7499587 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,014,242 A→T 14.1% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,014,2420AT14.1% 202.1 / 5.9 127intergenic (+88/‑256)bolA/tigtranscriptional regulator BolA/trigger factor
Reads supporting (aligned to +/- strand):  ref base A (62/44);  new base T (9/9);  total (74/53)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGA  >  NZ_CP022686/4014106‑4014380
                                                                                                                                          |                                                                                                                                           
cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGAGGGCCGTTATG‑TTTTTTATGGA‑ttt                                                                                                                                         >  2:452877/1‑140 (MQ=255)
 acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑tttt                                                                                                                                        >  8:521314/1‑140 (MQ=255)
 acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCGTTTCCAGTATGTTGCTAAAGATTTTATTAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGT‑tttt                                                                                                                                        >  6:76612/1‑140 (MQ=255)
  ccGGCTTTGCCTCTCATCCCTGTCGTGGAGCAGGAATCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATTTTGCTAAAGATTTTATGAAATAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTg                                                                                                                                       <  3:904499/140‑1 (MQ=255)
   cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTTTATGTG‑TTTTga                                                                                                                                      >  6:343702/1‑139 (MQ=255)
        ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCttt                                                                                                                                 >  6:583953/1‑140 (MQ=255)
        ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTATGGA‑TTTTGCGCttt                                                                                                                                 >  4:397617/1‑140 (MQ=255)
          gCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCGTTTCCTGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTATG‑TTTTTTGTGGG‑TTTTTCGCttttt                                                                                                                               >  7:183477/1‑140 (MQ=255)
             tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATTAAAAAC‑AGCCTGCGGGCCGTTTTG‑TTTTTTATTGT‑TTTTTCGCTTTTTTcc                                                                                                                            >  1:950385/1‑140 (MQ=255)
             tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGcc                                                                                                                            <  4:370704/140‑1 (MQ=255)
             tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGcc                                                                                                                            <  6:924731/140‑1 (MQ=255)
               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAATATTTTATGAAAAAC‑GGCCTGCGGGCCGTGTTG‑TTTTGTGTGGA‑TTTTGCGCGTTTTGCCCa                                                                                                                          >  5:635739/1‑140 (MQ=255)
               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGATAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGGGCTTTTTGCCCa                                                                                                                          >  8:154972/1‑140 (MQ=255)
               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTTGA‑TTTTTCGCTTTTTGaccc                                                                                                                          >  6:560876/1‑139 (MQ=255)
               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGT‑TTTTGCGCTTTTTGCCCa                                                                                                                          >  1:504862/1‑140 (MQ=255)
               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGGGCTTTTTTcccc                                                                                                                          >  7:72202/1‑139 (MQ=255)
               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCGTTTTGGCCa                                                                                                                          >  5:416894/1‑140 (MQ=255)
                      tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTT‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCa                                                                                                                   >  6:226720/1‑140 (MQ=255)
                      tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCCGCATTCa                                                                                                                   >  2:896396/1‑140 (MQ=255)
                           tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCAAGCATTCGGACGa                                                                                                              >  3:740955/1‑140 (MQ=255)
                           tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑GTTTGTCTGGA‑TTTTGCGCTTTTTGGCCAGCATTCatgcga                                                                                                              >  5:461190/1‑135 (MQ=255)
                             gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGaaa                                                                                                            >  5:187740/1‑140 (MQ=255)
                               gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTGGGCGCTTTTCCTGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGTCCTGCATTCAGACGaaatt                                                                                                          <  6:894950/140‑3 (MQ=255)
                                cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGGTTTTTGCCCAGCATTCAGACGAAAATc                                                                                                         >  1:137034/1‑140 (MQ=255)
                                cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATc                                                                                                         >  4:711582/1‑140 (MQ=255)
                                  ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTAAGACGAAAATCGc                                                                                                       >  8:455100/1‑140 (MQ=255)
                                  ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCGTTTTTCCCAGCATTCAGACGAAAATCGc                                                                                                       >  1:307808/1‑140 (MQ=255)
                                  ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTGTTG‑TTTTGTGTGGA‑TTTTGCGGGTTTTTGCCAGCCTTAAGACGaaaaaagc                                                                                                       >  7:490401/1‑136 (MQ=255)
                                    aaGCATCTCTTAAACACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGccc                                                                                                     <  4:61878/140‑1 (MQ=255)
                                     aGCATCGCGTAAAAACTCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCAGTTTTG‑TTTTGTGTGGA‑TTTTGCGGTTTTTGAGCCGCATTCAGACGAAAAGAGCCCg                                                                                                    >  7:210878/1‑140 (MQ=255)
                                     aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCAGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATAGCCCg                                                                                                    >  8:139783/1‑140 (MQ=255)
                                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTT‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGa                                                                                                 >  3:68174/1‑140 (MQ=255)
                                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTGGGGA‑TTTTTCGCTTTTTTGCCCGCATTCAGCCGAAAATCGCCCgggt                                                                                                 >  3:619876/1‑139 (MQ=255)
                                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGa                                                                                                 >  8:239507/1‑140 (MQ=255)
                                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTATGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATAGCCCGGGa                                                                                                 >  1:546962/1‑140 (MQ=255)
                                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTGTTG‑TTTTGTAGGGA‑TTTTTCGCTTTTTGGCCAGCATTCAGCCGAAAATCGCCCgggt                                                                                                 >  7:868756/1‑139 (MQ=255)
                                        aTCGCGTAAAAACGCATTTGCAACAGTCGGCGCTGTTACAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTGTTG‑TTTTGGCTGGA‑TTTTGCTCTTTTTTCCCAGCATTGAGCCGCAAATCGCCCgggt                                                                                                 >  6:279110/1‑139 (MQ=255)
                                            cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAATATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTGTGGAGTTTTTCCCTTTTTACCC‑GAATGAAGACGCAAATAGCCCGGGGAttt                                                                                             >  5:189940/1‑139 (MQ=255)
                                            cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTACAGTATGTTCCTAAAGATTTTATGAAAAAC‑GCCCTGCGGGCCGTTATT‑TTTTGTCTGGA‑TTTTGCGCTATTTCCCCAGCCTTCAGACGCATAACTCACGGGACTtg                                                                                             >  6:462152/1‑140 (MQ=255)
                                             gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCGTTTTGCCCAGCATTGAGACGAAAATCGCCCGGGAATtgg                                                                                            >  4:751390/1‑139 (MQ=255)
                                                 aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGaaa                                                                                        >  2:722588/1‑140 (MQ=255)
                                                 aaaCGCATTTGCAACTGTCGGCGCTTTTCAAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGTA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTGaaa                                                                                        <  8:870549/140‑1 (MQ=255)
                                                 aaaCGCATTTGCAACTGGGGGCGCTTTTCCAGTATGTTGATAAATATTTTATGAAAACC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGAATTCAGACGAAAATCGCCCGGGAATTGTGaaa                                                                                        <  1:446582/140‑1 (MQ=255)
                                                       aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTTGA‑TTTTGCGCTTTTTGGCCAGCATTCAGAAGAAAATCGCCCGGGAATTGTGAAAAAATaa                                                                                  >  2:457646/1‑139 (MQ=255)
                                                       aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTTACCAGCATTCAGCCGAAAATAGCCCGGGAATTGTTAAAAAATaa                                                                                  >  1:386440/1‑139 (MQ=255)
                                                       aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATAc                                                                                  >  4:905083/1‑140 (MQ=255)
                                                       aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCAGTCAGACGAAAATAGCCCGGGAAGTGGGAAAAAATAc                                                                                  >  8:166544/1‑140 (MQ=255)
                                                              aCTGTCGGCGCTTTTCCTGTTTGTGGCTAAAGATTTTATGAAAACC‑GGCCGGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTCAAAAAAACCGCGACAg                                                                           <  6:362161/140‑1 (MQ=255)
                                                              aCTGTCGGCGCTTTTCCATTATGTTGCTAAACATTTTATGAAAAAC‑GGCCTGGGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCCCCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAg                                                                           <  4:694676/140‑1 (MQ=255)
                                                                tggcGGCGCTTTTCCAGTATGTTGCTAATTATTTTATTAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGTA‑TTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGTAATTGTGAAAAAATCCGCGACAgcg                                                                         <  7:71734/137‑1 (MQ=255)
                                                                tGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAgcg                                                                         <  4:598665/140‑1 (MQ=255)
                                                                 gTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTGTGGA‑TTTTGCGCTTTTTGCCCCGCATTAAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGCCAgcgc                                                                        >  4:423532/1‑140 (MQ=255)
                                                                 gTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTTCGCTTTTTTCCCAGCATTCAGACGAAAATAGCCCGGGAATTGTGAAAAAATAAGCGACAgcgc                                                                        >  8:835197/1‑140 (MQ=255)
                                                                     gcgcTATTCCATGATTTTCCAAAATATTTTATCAAAAAC‑GGCGTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCAAGCATTCAGACGAAAATCGCCCGTGAATTGTTAAAAAATACGCGACAGCGCGCaa                                                                    <  1:429751/140‑1 (MQ=255)
                                                                      ggcTTTCCCAGTAGTTTGCTAAAGATTTTATGCAAACC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGTA‑TTTTGCGCTTTTGGCCCAGCATTCAGCCGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAt                                                                   <  6:229534/139‑1 (MQ=255)
                                                                      cgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAt                                                                   <  3:707254/140‑1 (MQ=255)
                                                                      cgcTTTTCCAGTATGTTGATAATCTTTTTATGAAAACC‑GGCCTGCGGGCGGTTTTG‑TTTTGTCTGGT‑TTTTGCGCTTTTTGCCCCGCATTCCGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAAt                                                                   <  1:642194/140‑1 (MQ=255)
                                                                         ttttCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTGTTG‑TTTTGTATTGA‑TTTTTTGGTTTTTTGCCCGCATTGAGACGAAAATAGCCCGGGTATTGTGAAAAAATACGCGACCGCCCCGAATAAc                                                                >  8:305575/1‑140 (MQ=255)
                                                                          tttCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTGTGGT‑TTTTTCGCGTTTTTGCCAGCATTAAGACGAAAAACGCCCGGGAATTTTTAAAAAATAACCGACCGCGGGCAATAAcc                                                               >  1:985387/1‑140 (MQ=255)
                                                                               aGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCCGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                                                          <  7:521314/140‑1 (MQ=255)
                                                                                 tATGTTGCTAAAGATTTTATCAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                                        <  3:214285/140‑1 (MQ=255)
                                                                                  ttGTTTCTAAATATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATGCAGACGAAACTCGCCCGGGAATTGGTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                       <  8:294557/139‑1 (MQ=255)
                                                                                  ttGTTGCTAAATTTTTTATGAAAAAC‑GGCCTGCGGCCCGTTTTG‑TTTTGTGTGGA‑TTTTGCGCTTTTTGCCCAGAATTCAGCCGAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                       <  2:24051/139‑1 (MQ=255)
                                                                                  ttGTTGCTAAAGATTTAATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGT‑TTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                       <  7:134822/139‑1 (MQ=255)
                                                                                  ttGTTGATAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                       <  2:355577/139‑1 (MQ=255)
                                                                                      tGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTGTGG‑GTTTTTCTGGA‑TTTTGAGCTTTTTTCCCGCCATTCATACGCAAAGCGCCCGGGTATTGTGTAAAAAAACGCGACCGCGCGCCAATACCGTTCTCgcggcg                                                   >  2:327645/1‑135 (MQ=255)
                                                                                        cTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCCGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCCATAACCGTTCTCGACTCATa                                                 >  1:922504/1‑140 (MQ=255)
                                                                                               ttttATTAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa                                          <  4:398927/140‑1 (MQ=255)
                                                                                                 ttattaAAACG‑GGCCTGCGGG‑CGTTTTGTTTTTGGCTGTT‑TTTTGCGTTTTTTGCCCAGAATTCAGACTAAAATCGCCCTTTAATTGGTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATg                                        <  3:323663/140‑1 (MQ=255)
                                                                                                  tttGAAAACC‑GGCCTGGGGGGCGTTTTG‑TTTTGAGTGGT‑TTTTGCGCTTTTTGCCCAGCATTCATACTAAAATCGCCCGGGAATTGTTAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  3:103736/138‑1 (MQ=255)
                                                                                                  tctGAAAACC‑GGCCTGCGGGCGGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCACCATTCAGACGAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  8:850721/138‑1 (MQ=255)
                                                                                                  tATTAAAACC‑GGCGTGGGGGCGGTTTTG‑TTTTGGCGGTT‑TTTTGCGCTTTTTGCCCAGCATCCAGCCCAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  8:45656/140‑1 (MQ=255)
                                                                                                  tATTAAAACC‑GGCCTGCGGGCGGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  3:423532/140‑1 (MQ=255)
                                                                                                  tATGAAAAAC‑GGCCTGCGGGCGGTTTTG‑TTTTGTCTGTA‑TTTTGCGGTTTTTGCCCAGCATTCAGACTAAAAGCGCCCGGGAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  3:36166/140‑1 (MQ=255)
                                                                                                  tATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTGTGGT‑TTTTTCGCTTTTTGCCCAGCATTCAGACGAAAATAGCCCGGGAATTGTGTAAAAATACGCGCCAGCGCGCAATAACCGTTCTCGACTCATTAAAGtgctgc                                       >  3:414467/1‑140 (MQ=255)
                                                                                                  tATGAAAAAC‑GGCCGGCGGGCGGTTTTG‑TTTGGGCTGTA‑TTTTGCGCTTTTGGCCCAGCATTCTGACTAAAATCGCCGGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  7:176100/140‑1 (MQ=255)
                                                                                                  tATCAAAACC‑GGCCGGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCCGCATTAAGCCTAAATTCGCCCGGGAATTGTCAAAAAAACCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  4:953111/140‑1 (MQ=255)
                                                                                                  tATCAAAAAG‑GGCCTGGGGGCGTTTTTG‑TTTTGTCTGTT‑TTTTGCTCTTTTTCCCCAGATTTCAGACTAAAATCCCCGGGTAATTTTTAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  5:124952/140‑1 (MQ=255)
                                                                                                  tATCAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCTAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  1:509276/140‑1 (MQ=255)
                                                                                                  aaTGAAAACC‑GCCCTGGGGGCCTTTTTG‑TTTTGTCTGGA‑TTTGGCGCTTTTTGCCCCCCATTCAGACTAAAATCGCCCGGCAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  7:111681/139‑1 (MQ=255)
                                                                                                     ataaaaCGGGCGTGCGGGCCTTTTTG‑TTTTGTCTGTA‑TTTTGCGGTTTTTGCCCTCCATTCAGACGAAAATCGCCCGGCAATTGTTAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCg                                     <  1:216523/138‑1 (MQ=255)
                                                                                                       aaaaC‑GGCCTGCGGGCCGTTTTG‑TTTTGTATGGT‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGCCAGAGCGCAATAAACGTTCTCGAAGCATAAAATTGATGCAGCtt                                  >  7:684831/1‑139 (MQ=255)
                                                                                                                gtgtgggCGGTTTTG‑TTTTGGGTGGT‑TTTTGCGCTTTTTGCCCAGCTTTAAGACTAAATTCGCCCGGCATTTGGTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                          <  1:337434/136‑1 (MQ=255)
                                                                                                                  gcgggcggTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                        <  2:530524/140‑1 (MQ=255)
                                                                                                                  gCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGCCGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                        <  6:391398/140‑1 (MQ=255)
                                                                                                                  gCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                        <  3:736551/140‑1 (MQ=255)
                                                                                                                    gggCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTc                      <  2:745343/140‑1 (MQ=255)
                                                                                                                     ggCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTcc                     <  4:64335/140‑1 (MQ=255)
                                                                                                                     ggCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATGGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTcc                     <  6:608461/140‑1 (MQ=255)
                                                                                                                      gCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCGTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTATCGCCTCATAAAAGTGATTCCGCTATAATGCCGCGTcca                    >  4:254542/1‑139 (MQ=255)
                                                                                                                             tg‑ttttgtCTGGT‑TTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa             <  7:375059/140‑1 (MQ=255)
                                                                                                                             tg‑tttgttCTGTT‑TTTTGCGGTTTTTCCCAAGCATACACACCAAAATCCCCCGGTAATTGTTAAAAAAACCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa             <  7:628610/140‑1 (MQ=255)
                                                                                                                                    ttCTGTA‑TTTTGCGATTTTTGCCCAGCATTCAGACTAAAATCGCCGGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt       <  1:913423/139‑1 (MQ=255)
                                                                                                                                    gtatGGA‑TTTTGCGATTTTTGCCCAGCATTCCGACGAAAATCGCCCGTGAATTGTGAAAATATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt       <  4:397850/137‑1 (MQ=255)
                                                                                                                                    gtCTGGA‑TTTTGCGCTTTTTGCACAGAATTCAGACTAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt       <  4:101168/140‑1 (MQ=255)
                                                                                                                                     tCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc      >  7:833102/1‑140 (MQ=255)
                                                                                                                                     tCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc      >  5:120182/1‑140 (MQ=255)
                                                                                                                                     tCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc      >  4:73013/1‑140 (MQ=255)
                                                                                                                                        tta‑TTTTCCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCggg   <  8:187792/138‑1 (MQ=255)
                                                                                                                                         gA‑TTTTGCGATTTTTGCCATGAATTCATACTAATATCGCCCGTGTTTTTTGAAAAAATACGCGACAGCGCTCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga  <  7:626416/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTG‑TTTTGTCTGGA‑TTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGA  >  NZ_CP022686/4014106‑4014380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A