Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F13 I0 R1	230	5035.0	9125430	98.4%	8979423	135.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	4,014,242	A→T	10.3%	intergenic (+88/‑256)	bolA → / → tig	transcriptional regulator BolA/trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	4,014,242	0	A	T	10.3%	338.0 / 4.0	176	intergenic (+88/‑256)	bolA/tig	transcriptional regulator BolA/trigger factor
Reads supporting (aligned to +/- strand): ref base A (74/78); new base T (10/11); total (87/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGAT  >  NZ_CP022686/4014106‑4014381

cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCGAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTTTCTTGAttt                                                                                                                                          >  6:114527/1‑140 (MQ=255)

cacCGTCTTTGCCTCTCCTCCCAGTCGAGGAGCAGGAAGCATCGCGTAAAAAAGCATTTGAAACTGTCGGCTCTATTCCAGTATGTTGCTAAAGATTTTATGAAAAACTACCTTCGGGCCG‑TGTTTTTTTGTCTGTAttt                                                                                                                                          >  6:885498/1‑140 (MQ=255)

 acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGAtttt                                                                                                                                         <  5:42328/140‑1 (MQ=255)

 acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTATGGTtttt                                                                                                                                         >  2:1070840/1‑140 (MQ=255)

  ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTg                                                                                                                                        <  2:803746/140‑1 (MQ=255)

     tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTTGATTTTgcgc                                                                                                                                     >  2:878160/1‑140 (MQ=255)

        ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGGCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCttt                                                                                                                                  <  3:677208/140‑1 (MQ=255)

          gCCTCTCCTCCCTGTCGTGGCGCAGGAAGCATCGCGTAATAACGCATTTGCAACTGTCGGCGCATTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTTTTTTGGCAGGATTTTGCGCgtttt                                                                                                                                >  2:639990/1‑140 (MQ=255)

             tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTg                                                                                                                               <  7:578288/138‑1 (MQ=255)

             tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGcc                                                                                                                             <  1:297200/140‑1 (MQ=255)

             tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGcc                                                                                                                             <  6:486748/140‑1 (MQ=255)

             tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTATGCGCTTTTTg                                                                                                                               <  7:811074/138‑1 (MQ=255)

               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTTTTTTTTATGGTTTTTTGGCttttttgcca                                                                                                                           >  5:398988/1‑135 (MQ=255)

               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCa                                                                                                                           >  2:665280/1‑140 (MQ=255)

               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TGTTGTTTTGTCTGGATTTTGCGCGTTTTGcccc                                                                                                                           >  4:860085/1‑139 (MQ=255)

               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCGTTTCCAGTATGTTGCTAAAGATATTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATGTTGCGCGTTTTGgccc                                                                                                                           >  6:784542/1‑139 (MQ=255)

                      tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                                                    >  2:1125105/1‑140 (MQ=255)

                        tCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGa                                                                                                                  <  4:586267/140‑1 (MQ=255)

                         cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGTCCTGCGGGCCG‑TGTTGTTTTGTCTTGTTTTTGCGGGTTTTGGCCGGCAATCAggc                                                                                                                 >  5:545730/1‑138 (MQ=255)

                         cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTTCGCATTTTGCCCAGCAATCAgcc                                                                                                                 >  1:470420/1‑138 (MQ=255)

                         cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGGTTTGTGTTGTTTTTTCGCTTTTTTGCCCCCATTGAGAc                                                                                                                 >  5:13144/1‑140 (MQ=255)

                             gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TGTTGTTTTTTCTGGTTTTTGCGGTTTTTGGCCAGCATTAAGACGa                                                                                                               >  7:161832/1‑138 (MQ=255)

                              aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaaa                                                                                                            >  2:628668/1‑140 (MQ=255)

                               gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTTTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaa                                                                                                             >  7:624422/1‑138 (MQ=255)

                                  ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTGTGGATTTTGCGGTTTTTGCCCCGCATTGAGACGAAAATAGc                                                                                                        >  1:140237/1‑140 (MQ=255)

                                  ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTTCGCTTTTTGCCCAGCATTCAGACGAAAATCGc                                                                                                        >  3:1007252/1‑140 (MQ=255)

                                  ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑ATTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTAAGACGAAAATCGc                                                                                                        >  2:347685/1‑140 (MQ=255)

                                  ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTATATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAATCAGACGAAAATCGc                                                                                                        >  2:342252/1‑140 (MQ=255)

                                  ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCGTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTGTTGTTTTGATTTTTTGCGTTTTGGCCACCATTCAGAAGCAAATAGc                                                                                                        >  3:576236/1‑140 (MQ=255)

                                       cATCGCGTTAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAATCGGCCTGCGGGCCC‑TGTTGTTTTGTCAGGATTTTGCGCATTGTGCCGAGCCTGCAGAGGAAATGCGCCCggg                                                                                                   >  6:474593/1‑140 (MQ=255)

                                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTTCGGGCCG‑TTTTGTTTTGTGGGGTTTTTGCGCTTTTTGCCCAGCATTAAGCAGATAATCGCCCgggg                                                                                                  >  3:989989/1‑139 (MQ=255)

                                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCTGGACG‑TTTTTTTTTGTCTGGATTTTTCGCTTTTTGACCAGCATTCAGACGAAAATCGCACGGGa                                                                                                  >  4:1045809/1‑140 (MQ=255)

                                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGCCGAAAATCGCCCgggt                                                                                                  >  6:498639/1‑139 (MQ=255)

                                         tCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTTGCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATCGCCCGGGaa                                                                                                 >  6:331180/1‑140 (MQ=255)

                                          cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTTTTTTTTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGGCGAAAATCGCCCGGGTAt                                                                                                >  4:79034/1‑140 (MQ=255)

                                          cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAAt                                                                                                <  5:507456/140‑1 (MQ=255)

                                             gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTTTCTGGATTTTGCGCTTTTTTTCCAGCAGTCAGACGAAAAACGGCCGGGTAtttt                                                                                             >  5:8021/1‑138 (MQ=255)

                                               aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTATGTCGGGTTTTTGCGCGTTTTGCCCAGCATTCACACGAAAATCGCCCGGGAATtgt                                                                                             >  8:485678/1‑138 (MQ=255)

                                                   ccGCATTTGCACCTGTCGGCGCTTTTCCAGTATGTTGCTTAAGATTTTATGAAAAACGGACTGCGGGCCG‑TTTTTTTTTCGGTTGATTTTGCGCTGTTTGACCAGCAGTCAATCGAAAATCGCCCGGTAATTGGGaaa                                                                                         >  8:67665/2‑138 (MQ=255)

                                                             aaCTGTGGGCGTTTTTCCTTTTTGTTGATAAATATTTTCTCAAAACCGGCGTGCGGGCCT‑TTTTGTTTTTTCTGGATTTGGCGCTTTTTGCCCACAATTCACACGAAAATCGCCCGGGAATTGTCAAAAAATACGCGa                                                                               <  8:583060/138‑1 (MQ=255)

                                                              aCTGTCGGCGCTTTTCCAGTATGTTGCAAAATATTTTATGAAAACCGGCCTGCGGGCGG‑TTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAg                                                                            <  4:651495/140‑1 (MQ=255)

                                                                tGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAgcg                                                                          <  5:1042210/140‑1 (MQ=255)

                                                                gggcGGCGATTTTCCAGTATGTTGCTAAAGTTTTTATGAAAACCGCCCTGCGGGCCG‑TTTTGTTTTGGCTGGATTTTGCGCTTTTTGCCAAGCATTCAGACGAAAAGCGCCCGGTAATTGTGAAAAAATCCGCGACAgcg                                                                          <  1:448403/137‑1 (MQ=255)

                                                                   cGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGTTTTTTCGCTTTTTGCCCCGCATTCAGACGAAAAAAGCCCGGGAATTGTGAAAAAATACCCGACAgcgc                                                                         >  8:570666/1‑138 (MQ=255)

                                                                    ggCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCTGGCCG‑TTTTGTTTAGTATGGAATTTGCCCTGTTTGCCCAGCAATAAGACGAAAATCGCCAGGGAATTGTGAAAAAATACGAGAAAACGCCCa                                                                      >  4:1141775/1‑140 (MQ=255)

                                                                    ggCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACCGCGCGCa                                                                      >  6:950392/1‑140 (MQ=255)

                                                                        cTTTTCCTGTATGTTGATAACTATATTAACAAAACCGGCCTGCGGGCTG‑TTTTGTTTTGTCGTGTTTTTGCGGTTTTTGCCCCCCATTCAGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATaa                                                                  <  3:594463/140‑1 (MQ=255)

                                                                        cTTTTCCATTATGTTGCTAAAGATTTTATTAAAAACGCCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATaa                                                                  <  4:212371/140‑1 (MQ=255)

                                                                        cTTTTCCAGTATGTTCCTAAATATTTAATCAAAAACGGCCGGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCATCATTCACACCAAAATCGCCCGGGAATTGTGAAAAAATCCGCGACAGCGCGCAATaa                                                                  <  3:40513/140‑1 (MQ=255)

                                                                         ttttCCATTTTGTTGCAAAATATTTTATTAAAACCGGCCTGCGGGCCT‑TTTTTTTTTGTGTGGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGTAATTGTCAAAAAATACGCGACAGCGCGCAATa                                                                   <  7:740503/138‑1 (MQ=255)

                                                                                 tttGTTGCTAAAGATTATATGAAAACCGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCAAGCATTCAGACTAAAATCCCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                                         <  6:768763/138‑1 (MQ=255)

                                                                                 tATGTTGCTAATGATTTTATGAAAAACGGCCTGGGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCTGACGAAAATCGCCCGGTATTTGTTAAAAAATACGCGACAGCGCGCAATAACCGTtct                                                           <  8:100488/138‑1 (MQ=255)

                                                                                 tATGTTCCAAAAAATTTTATCAAAAACGCCCTGGGGGCGG‑TTTTTTTTTGTCTGTATTTGGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCGGGTAATTTTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                                         <  3:926746/140‑1 (MQ=255)

                                                                                 tATGTGTCTAATGATTTTATGAAAAACGGCCTGCGGTCCG‑TTTTGTTTTGTCTGGTTTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTTTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                                                           <  8:100490/138‑1 (MQ=255)

                                                                                 tATGTGGCTAAATATTTTATGTAAACCGGCCGTCGGGCGT‑TTTTGTTTGGTCTGGATTTTGCGCTTTTTGCCCCGCATTCCTCCAAAAATCGCCCGGTAATTTTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                                                           <  7:365520/138‑1 (MQ=255)

                                                                                 tATGTGGATAAATATTTTATGAAAAACGGCCGGCGGGCCG‑TTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                                         <  3:470098/140‑1 (MQ=255)

                                                                                  ttGTTGCTAAATTTTTTATTAAAAACGGCCGGCGGTCCG‑TTTTGTTTTGTCTGTATTTTGCGCTTTTTCCCCACAATTCAGACGAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                        <  5:1107514/139‑1 (MQ=255)

                                                                                  aTGTTGATAACTATTTTATGAACACCGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                        <  2:1159306/140‑1 (MQ=255)

                                                                                   tGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGAGTTTGCGCTTTTTGCCCAGCATTAAGACGAAAATCGAGCGCGAAATGTGAAAAAATACGCGACATCGGGCAATAACCCTTCTCGAc                                                       >  2:171203/1‑140 (MQ=255)

                                                                                     ttGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTGTGGGTTTTGCGCGTTTTGCCCCGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCCTTCTCGACTc                                                     >  1:480833/1‑140 (MQ=255)

                                                                                     ttGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TGTTGTTTTGTGTGGATTTTGCGCTTTTTTCCCAGCCTTCAGACGAAAATAGGCCGGGAATTGTGAAAAAATACCCGACAGCGCGCAATAACCGTtctcccctc                                                     >  3:193775/1‑135 (MQ=255)

                                                                                     ttGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TGTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCACACGAAAAACGCCCGGGAATTGTGAAAAAAAACGCGCCAGCGCGCAATAACCGTTCTCGACTc                                                     >  2:493576/1‑140 (MQ=255)

                                                                                       gCTAAATATTTAATGAAAACCGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCAt                                                   <  1:886373/140‑1 (MQ=255)

                                                                                        cTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTTGAATTTGCGGTTTTTGCCCCACGATCAGCCGAAAATAGCCCGTGAAATGTGAAAAAATACGCGCCACCGCGCAATAACCGGTCACGACTCa                                                    >  8:1043290/1‑138 (MQ=255)

                                                                                        cTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTG‑AAAAATACCCGACAGCGCGCCATAACCGTTCTCGACTCATaa                                                 >  2:736359/1‑140 (MQ=255)

                                                                                        cTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TGTTTTTTTGTCTGGATTTTGGGCTTTTTGGCCAGCATTGAGACGCAAAAAGCCCGGGAATTGTGAAAAAATACGCCACAGCGCGCAATAAACGTTCTCGACTCa                                                    >  7:421832/1‑138 (MQ=255)

                                                                                        cTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TGTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCCGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTAGCGACTCaaa                                                  >  5:997798/1‑138 (MQ=255)

                                                                                         tAAAGATTTTATGAAAAACGGCCGGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaa                                                 <  4:152939/140‑1 (MQ=255)

                                                                                               ttttATTAAAAACGGCCTGCGGGCAG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATGCAGACGAAAATCGCCGGGGAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                             <  8:748124/138‑1 (MQ=255)

                                                                                                tttATGAAAAACGGCCTGCGGGCCG‑TGTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAAAACCGGTCTAGCCTCATAAAAGTGAt                                          >  6:1053470/1‑140 (MQ=255)

                                                                                                  tATTAAAACCGGCCTGCGGGCCT‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCCGCATTCATACTAAAATCGCCCGGGAATTGTTAAAAAATACTCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  2:516692/140‑1 (MQ=255)

                                                                                                  tATTAAAAACGGCCTGCGGGCCT‑TTTTTTTTTGTCTGTATTTTGCTCTTTTTTCCCAGCATTCAGACGAAAATCGCCCGGTAATTTTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  5:571095/140‑1 (MQ=255)

                                                                                                  tATGAAAACCGCCCTGCGGGCGT‑TTTTGTTTTGTCGGTTTTTTGCGCTTTTTGCCCCGCATTCACACGAAAATCCCCCGGGATTTTGGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  6:498610/140‑1 (MQ=255)

                                                                                                  tATGAAAAACGGCCTGCGGGCGG‑TTTTGTTTTGTATGTATTTTGCGCTTTTTGCCCTGAATTCAGCCTAAAACCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  5:293329/140‑1 (MQ=255)

                                                                                                  tATGAAAAACGGCCTGCGGGCCG‑TTTTTTTTTGGCTGGATTTTGCGCTTTTTGCCCAGCATTCAGCCGAAAATCGCCCGGGACTTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  5:401787/140‑1 (MQ=255)

                                                                                                  tATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  6:134378/140‑1 (MQ=255)

                                                                                                  tATCAAAAAGGGCCTGGGGGCGT‑TTTTTTTTTGTCGTGTTTTTGCGCTTTTTCCCCACAATGCATACGAAAATCGCCCGGTAATTGTCAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  5:1010687/140‑1 (MQ=255)

                                                                                                  atttaaaaaCGGCGTGCGGGCCG‑TTTTGTGTGGTGTGGATTTTGCGGTTTATGCCAAGAATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  2:448863/136‑1 (MQ=255)

                                                                                                   aataaaaaCCGGCTGGGGGGCGTTTTTTTTTTGTCTGTATTTTGGGCTTTTTCCCCCCAATTCAGACTAAAATCCCCGGTTATTTGTCAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  1:797021/137‑1 (MQ=255)

                                                                                                         accGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTCAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCtata                                 <  5:643780/138‑1 (MQ=255)

                                                                                                               gtGCGGGCGT‑TTTTTTTTGGGCTGGTTTTGGCGCTTTTTGCCCCGCTTTCAGACGAAAATCGCCGGGGAATTGTTAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                           <  2:174027/139‑1 (MQ=255)

                                                                                                               cTGCGGGCCG‑TTTTGTTTGGTCTGGATTTTGCGCTTTTTGCCCAGAATTCAGACGAAAATCGCCCGGTACTTGGGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                           <  5:993168/140‑1 (MQ=255)

                                                                                                                ggCGGGCCG‑TTTTGTTTTGTGTGGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAACTCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc                            <  8:34450/137‑1 (MQ=255)

                                                                                                                 gCGGGCCT‑TTTTGTTTTGTCTGGATTTTGCGCTGTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                         <  3:122009/140‑1 (MQ=255)

                                                                                                                 gCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                         <  2:412133/140‑1 (MQ=255)

                                                                                                                   gttagg‑ttttgttttGTCTTTTTTTTGAGCTTTTTGCCCACCTTTCAGACTAAAAGCGCCCGGGATTTGTGAAAAAATACGCGCCAGAGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTc                       <  2:673223/135‑1 (MQ=255)

                                                                                                                    gtccG‑TTTTGTTTTTTGTGTTTTTTGCGTTTTTTGCCAAGCATTCATACGAAATTCGCCGGTTATTTGTTAAAAAATACGCGACAGCGCTCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTcc                      <  1:348296/138‑1 (MQ=255)

                                                                                                                     gCCG‑TTTTGTTTTGTCTGGATTTTTCGCTTTTTGCCCAGCATTCAGCCGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGCCTCATAAAAGTGATGCCGCGATAATTCCGCGGCCt                     >  1:841605/1‑140 (MQ=255)

                                                                                                                           ttttttttgtCTGGATTTTTCGCTTTTTGCCCAGCAATAAGACGAAAATCGCCCGGGAATTTTGAAAAAATAAACGACAGCGCGCAATAAACGTTGTCGACACATAAAAATGATGCCGATATAATTACGCGTCCTaat                  >  8:910417/1‑136 (MQ=255)

                                                                                                                             ttttttgtCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa              <  4:142724/138‑1 (MQ=255)

                                                                                                                             tgttttttCTGTATTTTGCGATTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa              <  6:876780/140‑1 (MQ=255)

                                                                                                                             tgttttgtCTGGATTTTGCTCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa              <  4:420429/140‑1 (MQ=255)

                                                                                                                             tgttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa              <  4:708798/140‑1 (MQ=255)

                                                                                                                              tttttgtCTGGTTTTTGCGCTTTTTGCCCAGCATTCCGACTAAAATCGCCCGGTATTTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTACAATGCCGCGTCCTATTTGAAt             <  5:429120/139‑1 (MQ=255)

                                                                                                                                   gtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGTCGCGTCCTATTTGAATGCttt        <  1:665280/140‑1 (MQ=255)

                                                                                                                                   gtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt        <  4:1090616/140‑1 (MQ=255)

                                                                                                                                    tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGCTGCCGCTATAATGCCGCGTCCTATTTGACTGCTTTc       >  5:284390/1‑140 (MQ=255)

                                                                                                                                    tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAACGCCGCGTCCTATTTGAATGCTTTc       >  1:626342/1‑140 (MQ=255)

                                                                                                                                        taTTTTGCTTTTTTTGCCCAGAATTCAGAGTAAATTCCCCCGGTATTGGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTGCGGga   <  6:425356/139‑1 (MQ=255)

                                                                                                                                         aTTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATCCCCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgat  >  6:316130/1‑140 (MQ=255)

CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑TTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGAT  >  NZ_CP022686/4014106‑4014381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A