Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F13 I0 R1	203	11425.2	8793538	98.4%	8652841	135.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	4,014,242	A→T	12.5%	intergenic (+88/‑256)	bolA → / → tig	transcriptional regulator BolA/trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	4,014,242	0	A	T	12.5%	273.2 / 3.4	152	intergenic (+88/‑256)	bolA/tig	transcriptional regulator BolA/trigger factor
Reads supporting (aligned to +/- strand): ref base A (68/59); new base T (13/5); total (88/64)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGA  >  NZ_CP022686/4014106‑4014380

cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTGTGGAttt                                                                                                                                         >  7:364536/1‑140 (MQ=255)

cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGAttt                                                                                                                                         >  6:940210/1‑140 (MQ=255)

cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGAatt                                                                                                                                         >  6:429169/1‑140 (MQ=255)

cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑GTTGTTTTGTCTGGAttt                                                                                                                                         >  5:646254/1‑140 (MQ=255)

cacCGTCTTTGCCTCTCCACCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACAGTCGGCGCTTTTACAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑G‑GTTGTTTTGTCTGGAatt                                                                                                                                         >  2:489315/1‑140 (MQ=255)

 acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTTGAtttt                                                                                                                                        >  2:1114560/1‑140 (MQ=255)

 acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGAtttt                                                                                                                                        >  1:448813/1‑140 (MQ=255)

 acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGAtt                                                                                                                                          >  3:855334/1‑138 (MQ=255)

 acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCAAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCTGGCCG‑T‑TTTTTTTTTTCTGGAtttt                                                                                                                                        >  7:883099/1‑140 (MQ=255)

   cGTCTTTGCCTCTCCTCCCTGTCGTTGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTgc                                                                                                                                      >  8:514828/1‑140 (MQ=255)

   cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTTGATTTTgg                                                                                                                                      >  5:252456/1‑139 (MQ=255)

   cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTgc                                                                                                                                      <  7:258854/140‑1 (MQ=255)

     tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTgg                                                                                                                                      >  3:1113484/1‑137 (MQ=255)

     tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑GTTGTTTTTTGTGGGTTTTtcgc                                                                                                                                    >  5:416175/1‑140 (MQ=255)

     tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTATTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑GTTGTTGTGTCCGGCTTTTgagc                                                                                                                                    >  6:991045/1‑140 (MQ=255)

       tttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTTTTTTGTCTGGAtttttggc                                                                                                                                    >  3:800015/1‑134 (MQ=255)

            ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTATGGATTTTGCGCttttt                                                                                                                               >  4:148247/1‑138 (MQ=255)

            ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGGCTGGATTTTGCGCttttt                                                                                                                               >  3:132875/1‑138 (MQ=255)

             tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTg                                                                                                                              <  3:64334/138‑1 (MQ=255)

             tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGcc                                                                                                                            <  5:994122/140‑1 (MQ=255)

               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTTTTTTGTATGGTTTTTGCGCGTTTTTCCCa                                                                                                                          >  6:171527/1‑140 (MQ=255)

               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGGGTTTTTCCCa                                                                                                                          >  1:889843/1‑140 (MQ=255)

               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAAGGCCTGCGGGCCG‑TGTTTGTTTT‑TCTGGTTTTTGCGGttttttgccc                                                                                                                          >  7:564249/1‑135 (MQ=255)

                 ctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑GTTTTTTTGTCTGTATTTTGCGCTTTTTTCCCAGc                                                                                                                        >  5:206893/1‑140 (MQ=255)

                 ctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCGTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCAGGATTTTGGGCGTTTTGcccc                                                                                                                          >  3:982878/1‑137 (MQ=255)

                 ctccCTGTCGTGGAGCACGAAGCATCGCGTAAAAACGCATTTGAAACTTTCGGCGCTTTTCCAGTATGATGCTAAAGATTTTAAGAAAAACGGCCTCCGGGCCG‑G‑TTTGTTTTTTCTGGATTTTGCCCttttttcaca                                                                                                                          >  4:305077/1‑133 (MQ=255)

                    ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAtt                                                                                                                     <  2:464918/140‑1 (MQ=255)

                    ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑GTTGTTTTGTCTGGATTTTTCGGGTTTTGCCCAGCAtt                                                                                                                     >  1:84505/1‑140 (MQ=255)

                         cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCTACTGTCGGCGCATTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTTTATGGTTTTTGCGCGTTTTGCCCACCATTCAgcc                                                                                                                >  7:255166/1‑138 (MQ=255)

                         cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTTTTAGGATTTTTGGCATATTTGCCAGCATTAAGAc                                                                                                                >  1:426493/1‑140 (MQ=255)

                            ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaa                                                                                                             <  6:561217/140‑1 (MQ=255)

                             gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTGTGGTTTTTGGGCGTGTTGGCCAGCATTCAGACGaaa                                                                                                            >  6:500259/1‑140 (MQ=255)

                                  ggAAGCATCGCGTAAAAACGTATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGc                                                                                                       >  6:314853/1‑140 (MQ=255)

                                  ggAAGCATCGCGTAAAAACGCATTTGCGACTGTCGGCGCTTTTCCAGTATTTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCCGCCGAAAATCGc                                                                                                       >  5:460459/1‑140 (MQ=255)

                                  ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGc                                                                                                       >  8:203991/1‑140 (MQ=255)

                                  ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTATGGTTTTTGCGCGTTTTGCCCCGCATTCAGCCGAAAATAGc                                                                                                       >  5:589929/1‑140 (MQ=255)

                                    aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTTAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑GTTGTTTTGTTTGGGTTTTTGGCTTTTTTCCCCACATTCAGACGCCAATctc                                                                                                       >  3:701744/1‑136 (MQ=255)

                                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAATGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑GTTGTTTTGTCTGGATGTTGCGCTTTTTGCCCCGCATTCAGACGAAAATCGCCCgggg                                                                                                 >  1:637835/1‑139 (MQ=255)

                                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTTTGGGGTTTTTGGGCGTTTTGCCCAGCATTAAGACGAAAATCGGCCgggt                                                                                                 >  1:546938/1‑139 (MQ=255)

                                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTATGGATTTTGCGCTTTTTGCCCAGCATTAAGACGAAAATCGCCCGGGa                                                                                                 >  2:336616/1‑140 (MQ=255)

                                        aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑G‑TTTGTTTTGTCGGGATTTTGCGCGTTTTGCACAGCATTCAGCCGACAATCGCCCGGGa                                                                                                 >  2:265027/1‑140 (MQ=255)

                                        aTCGCGTAAAAACGCATTTGCAACTGTCGCCGCTTTTCCAGAATTTTGATAAAGATTTTATGAAAAACAGCCTGCGGGCCG‑T‑TATGTTTTTTATTAATTATGCTCTTTTTGCCCTGCATTCAGACGAAAATCGcaacgga                                                                                                 >  6:701335/1‑134 (MQ=255)

                                           gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTTAAGATTTTATGAAAAACGGCCTGCGGGCCG‑G‑TGTGGTTTGTGTGGTTTTTGCGCTTTTTGCCCCGCATTCAGACGATAATCGGCCGGGAAtt                                                                                              >  5:191120/1‑140 (MQ=255)

                                             gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTGTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATtgt                                                                                            >  2:78807/1‑140 (MQ=255)

                                             gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATtgt                                                                                            >  2:783874/1‑140 (MQ=255)

                                              tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATtg                                                                                             >  4:803468/1‑138 (MQ=255)

                                              tAAAAACGCATTTGAAACTGTCGGCGATTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATtg                                                                                             <  3:429480/138‑1 (MQ=255)

                                               aaaaaCTCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATtgt                                                                                            <  3:698670/138‑1 (MQ=255)

                                                 aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGCCGAAAATCGCCCGGGAATTGTGaaa                                                                                        >  6:240782/1‑140 (MQ=255)

                                                   aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGaaa                                                                                        >  4:84703/1‑138 (MQ=255)

                                                           gCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGAGTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCCGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATAcgc                                                                                <  4:960128/138‑1 (MQ=255)

                                                              aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAg                                                                           <  7:758677/140‑1 (MQ=255)

                                                                tGTCGGGGCTTTTCCAGTTTTTTTCTAAATATTTTATGAAAACCGGCGTGGGGGGCT‑T‑TTTGTTTTGTCTGGTTTTTGCCCTTTTTGCCCAGCTTTCAGACGAAAATCGCCCGGTAATTGTGAAAAAATCCGCGACAgcg                                                                         <  6:789824/140‑1 (MQ=255)

                                                                tGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAgcg                                                                         <  2:821785/140‑1 (MQ=255)

                                                                tGTCGGCGCTAATCACGACTGTGGCTAACGATTTTAGGAAAAACGCCCTGCGGGCCG‑T‑TTTGTTTTGGCTGGATTTCGCGCTTTTTGCCCCGCTTTCAGACGAAAATCGCCCGGGAAGTGTGAAAAAATACGCGACAgcg                                                                         <  8:514237/140‑1 (MQ=255)

                                                                 gTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTTCGCTTTTTGGCCAGCATTCAGACGAAAATCGCCCGGGTATTGTGAAAAAAAAcgcgcccgccc                                                                        >  1:245357/1‑133 (MQ=255)

                                                                 gTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGGCCCGCATTCAGACGAAAATCGCCCGGGAATTTTGTAAAAATACGCGACAgcgc                                                                        >  2:388065/1‑140 (MQ=255)

                                                                   cGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAgcgc                                                                        >  4:426269/1‑138 (MQ=255)

                                                                   cGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAGTACGACGAAAAACGCCCGGGTAGTGTGAAAAAAAACGCGACAgcgcgc                                                                      >  6:239742/1‑140 (MQ=255)

                                                                                  ttGTTGCTAACTATTTTATGAAAACCGGCCTGCTGGCCG‑T‑TTTGTTTTTTCTGTATTTTGCGCTTTATGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTtctc                                                         <  3:648351/137‑1 (MQ=255)

                                                                                  ttGTTGCTAAATTTTTTTTTAAAAACGGCGAGCGGGCCG‑T‑TTTGTTTTGTCTTCATTTTGCTCTTTTTGCCCACCATACAGACTAAAAACGCCCGGTAATTGTTAAAAATAACGCGACAGCGCGCAATAACCGTtctc                                                         <  3:652899/137‑1 (MQ=255)

                                                                                     ttGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTGTGGTTTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGCCAGCGCGCAATAACCGGTCTCgcc                                                      >  3:490166/1‑136 (MQ=255)

                                                                                     ttGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTc                                                    >  6:138988/1‑140 (MQ=255)

                                                                                      tGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTGTCGACTCa                                                   >  5:875818/1‑140 (MQ=255)

                                                                                       gCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑GTTGTTTTGTCTGGATTTTGCGCGTTTTGCCCAGCATTAACACGAAAATCGCCCGGGAATTGTGAAAAAATAAGCGACCGCGCGCAAAAACCCTGCTCGCCTc                                                    >  4:308518/1‑138 (MQ=255)

                                                                                           aaGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTGTGGTTTTTGGGCTTTTTTGCCAGCATTCAGACGCAAATCGCCCGGGAATTGTGAAAAAATACCCGACAGCGCGCAATAACCGTTCTCGCCGCaaaaaa                                              >  1:1083645/1‑140 (MQ=255)

                                                                                           aaGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑GTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa                                              >  8:166389/1‑140 (MQ=255)

                                                                                                tttaataaaaaCGGCCTGCGGGCGT‑T‑TTTGTTTTGTCTGTATTTTGCGGTTTTTGCCCAGCATTCACACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                         <  1:90798/134‑1 (MQ=255)

                                                                                                  tATTAAAAACGGCCTGCGGGCGG‑T‑TTTGTTTTGTCTGTTTTTTGCGCTTTTTGCCCAGCATTCAGCCTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                         <  3:781081/138‑1 (MQ=255)

                                                                                                  tATTAAAAACGGCCTGCGGGCGG‑T‑TTTGTTTTGTCTGTTTTTTGCGCTTTTTGCCCAGAATTCAGACTAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  5:940210/140‑1 (MQ=255)

                                                                                                  tATTAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCTTTCAGACCAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  1:1114560/140‑1 (MQ=255)

                                                                                                  tATTAAAAACGGCCGGCGGGCGG‑T‑TTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCCGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                         <  4:497337/138‑1 (MQ=255)

                                                                                                  tATTAAAAACGGCAGGCGGGCGG‑T‑TTTGTTTTGTCTGTATTTTGCGATTTTTGCCCAGCTTTCAGACTAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                         <  3:737659/138‑1 (MQ=255)

                                                                                                  tATGAAAACGGGCTTGCGGGCGG‑T‑TTTGTTTTGTCGGGATTTTGCGCTTTTTGCCCAGCATTCCGCCGAACATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  6:14204/140‑1 (MQ=255)

                                                                                                  tATGAAAAAGGGCGTGCGGGCGG‑T‑TTTGTTTTGTCTGCATTTTGCGCTTTTTCCCCAGCTTTCAGACTAAAATCGCCCGGTAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  8:912231/140‑1 (MQ=255)

                                                                                                  tATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTGTGGATTTTGCGCGTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGGATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       >  1:203099/1‑140 (MQ=255)

                                                                                                  tATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGAATTCATACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                       <  5:314853/140‑1 (MQ=255)

                                                                                                  tATCAAAAACGGCCTGCGGGCGT‑T‑TTTTTTTTGTCTGGATTTTGCTCTTTTTGCCCAGAATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTACTCGACTCATAAAAGTGATGc                                       <  8:1020334/140‑1 (MQ=255)

                                                                                                    ttaaaaaCGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCg                                     <  6:333479/138‑1 (MQ=255)

                                                                                                        aaaCGGCCTGCGGGCCG‑T‑TTTGTTTTGTGTGGGTTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGTATTGTGAAAAAATACGCGACAGCGCGGAATAACCGTTGTCGACTCATAAAAGTGATGCCGCtat                                 >  5:354440/1‑140 (MQ=255)

                                                                                                             gCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGc                            <  7:273309/140‑1 (MQ=255)

                                                                                                                 gCGGGCTT‑T‑TTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGCCTAAAATCGCCCGGGAATTTTGAAAAAATCCGAGACAGCGCGAAATAACCGTTATCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                        <  7:419492/140‑1 (MQ=255)

                                                                                                                  gcggCCGTT‑TTTGTTTTTTGTGTATTTTGTCTTTTTTGCCCCGCATACAGACTAAAATGCCCCGGTAATTGGGAAAACATCCGCGACAGCGCGCAATAACCGTTCTCGACTCAAAAAAGTGATGCCGCTATAATGCcgcg                        <  7:255489/138‑1 (MQ=255)

                                                                                                                   gggCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                        <  3:618161/138‑1 (MQ=255)

                                                                                                                   gggCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTc                      <  2:443975/140‑1 (MQ=255)

                                                                                                                   gggCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCACACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTc                      <  2:203099/140‑1 (MQ=255)

                                                                                                                    gggcgtt‑tttgttttgTCTGTATTTTCGGCTTTTTGCCCAGATTTCAGACTAAAATGGCCGGGTATTTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAGTAATAAAAGTGATGCCGCTATAATGCcgcg                        <  4:1113484/132‑1 (MQ=255)

                                                                                                                        g‑t‑tttgttttGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATCGCCCGGGAAATGTGAAAAAATACGCGACACCGCCCAATAACCGTTATCCACTCATAAAAATGAATCCGCTATAAATCCGCCTCCTAtt                 >  2:624069/1‑140 (MQ=255)

                                                                                                                             ttgttttgtCTGTATTTTGCGCTTTTTGCCCATCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGGCCTATTTGa              <  2:127288/140‑1 (MQ=255)

                                                                                                                             ttgttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGa              <  2:889843/140‑1 (MQ=255)

                                                                                                                              ttttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa             <  2:634227/138‑1 (MQ=255)

                                                                                                                              tgttttgtCTGCATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAGCGCCCGGGAATTGTCAAAAAATACGCCACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa             <  8:100324/140‑1 (MQ=255)

                                                                                                                                    gtCTGTATTTTGCGCTTTTTGCCCAGCATTCATACGAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt       <  6:585513/140‑1 (MQ=255)

                                                                                                                                    gtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt       <  1:783874/140‑1 (MQ=255)

                                                                                                                                     tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGCCTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc      >  5:296558/1‑140 (MQ=255)

                                                                                                                                     tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc      >  1:401563/1‑140 (MQ=255)

                                                                                                                                       tGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc      >  4:763438/1‑138 (MQ=255)

                                                                                                                                         taTTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCgg    <  3:682174/137‑1 (MQ=255)

                                                                                                                                         taTTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTTTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga  <  5:782656/139‑1 (MQ=255)

                                                                                                                                         gATTTTGCGATTTTTCCCCAGAATTCAGACTAAAATCGCCCGGTATTTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga  <  6:771281/140‑1 (MQ=255)

CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCG‑T‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGA  >  NZ_CP022686/4014106‑4014380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A