Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F2 I0 R1 252 7301.5 9636800 98.5% 9492248 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,014,242 A→T 10.9% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,014,2420AT10.9% 316.0 / 4.0 168intergenic (+88/‑256)bolA/tigtranscriptional regulator BolA/trigger factor
Reads supporting (aligned to +/- strand):  ref base A (68/77);  new base T (10/10);  total (81/87)
Fisher's exact test for biased strand distribution p-value = 8.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGAT  >  NZ_CP022686/4014106‑4014381
                                                                                                                                         |                                                                                                                                             
cacCGTCTTTGCCTCTCCTCCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACTGCCTGCGGGCCGTTTTGTTTTTTGTGGAttt                                                                                                                                            >  8:899440/1‑140 (MQ=255)
 acCGTCTTTTCCTCTACTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAACA‑CGCATTTGCAACTGTCGGCTCTTTTCCAGTATTTTTCTAAAGATTTTATGAAAAACGTCCTGCGGGCCGTTTTGTTTTGTCTGGAtttt                                                                                                                                           >  6:64562/1‑140 (MQ=255)
   cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgg                                                                                                                                         >  7:259120/1‑139 (MQ=255)
   cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTAAGGATTTTgg                                                                                                                                         >  8:10574/1‑139 (MQ=255)
     tCTTTGCCTCTCCTCCCTGTCGTGGCGCAGGAAGCATCGCGTAAAAA‑CGCATATGCAACTGTCGGCGCGTTTCCAGGATGTTGCTAAAGATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTtcgc                                                                                                                                       >  6:724638/1‑140 (MQ=255)
     tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgagc                                                                                                                                       >  7:719913/1‑140 (MQ=255)
     tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCGTGTTTTTgg                                                                                                                                         >  1:395484/1‑137 (MQ=255)
        ttGCCTCTCCTCCCTGTCGTGGAGCAGGACGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCGGCGGGCCGTTTTGGTTTGTCTGGATTTTGCGC‑ttt                                                                                                                                   >  8:359306/1‑140 (MQ=255)
        ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑ttt                                                                                                                                   >  6:310772/1‑140 (MQ=255)
        ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑ttt                                                                                                                                   >  4:1113170/1‑140 (MQ=255)
           ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCGTTTCCAGTATGTTGCTAAAGCTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGCGC‑tttttt                                                                                                                                >  8:614674/1‑139 (MQ=255)
             tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTg                                                                                                                                <  2:1036360/138‑1 (MQ=255)
             tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTg                                                                                                                                <  1:393234/138‑1 (MQ=255)
             tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGcc                                                                                                                              <  5:310772/140‑1 (MQ=255)
             gctactccCTTGCCTGGAGCCTGTAGCATCCCGGAAAAACCGCA‑TTGCAACTTGCGGCGCTTTTCCAGTTTGTTGCAAAAGATTTTTTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGcc                                                                                                                              <  4:781807/136‑1 (MQ=255)
               tcctccCTGTCGTGGAGCAGGCAGCATCGCTTAAACA‑CTACTTTGCAACTGTATGCGCTGTTCAATTATGTTGATAAAGATTTTATGAAAAACGGCATGCGTGACGTTTTTTTTTGTGTTTTTTTTGGGC‑tttttttcca                                                                                                                            >  8:635499/1‑135 (MQ=255)
               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTTTGGTGCTAAAGATGTTATGAAAAACGGCCTGCGGGCCGTGTTGGTTTGTCTGGTTTTTTCGC‑TTTTTGcccc                                                                                                                            >  7:754441/1‑139 (MQ=255)
               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGATTTTGCGC‑TTTTTGCCCa                                                                                                                            >  4:572853/1‑140 (MQ=255)
               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTATGGATTTTTCGCGTTTTTGccc                                                                                                                             >  3:282843/1‑140 (MQ=255)
               tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACGGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCGTGCGGGGCGTGTTTTTTTGTGTGGTTTTTGGGG‑TTTTTGccac                                                                                                                            >  7:586545/1‑138 (MQ=255)
                 ctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCa                                                                                                                            >  2:698904/1‑138 (MQ=255)
                    ccTGTCGTGGAGCATGAAGGATCGCGTAAACA‑GGCATTTGCTACTGTCGTCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATATTGCGC‑TTTTTGCACAGCAt‑t                                                                                                                      <  6:367698/140‑1 (MQ=255)
                      tGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTATATGAAAAACGGCCTGCGGGCCGTTTTGTTTTAGAGGGATTTTGGGA‑TTTTTTCCCAGAAt‑tag                                                                                                                    >  6:963330/1‑138 (MQ=255)
                        tCGTGGAGCAGGAAGCAACGCGTAAAAA‑CGCATTTGCAACTGTCCGCGCTTTTCCAGGATGTTGCTAAAGATTTTATGAAAAACGTCCTGCGGGCCGTTTTGATTTTGCTGGATTTTTCGG‑TTTTTGCCCAGCaataa                                                                                                                     >  1:635506/1‑134 (MQ=255)
                         cGTGGAGCAGGATGCATCGCGTAAAAA‑AGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGCACTGCGGGCCGATTAGTGTTGGCTGGTTTTTGCGC‑TTTTTGCACAGCAT‑GCaccc                                                                                                                 >  8:660784/1‑137 (MQ=255)
                           tGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTGTCTGGATTTTGCGC‑TTTTTTCCCCGCAT‑TGAGACGa                                                                                                               >  7:1072424/1‑140 (MQ=255)
                           tGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTTCCCAGCAT‑TCAGACGa                                                                                                               >  6:553557/1‑140 (MQ=255)
                           tGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGa                                                                                                               >  6:1000214/1‑140 (MQ=255)
                             gAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTCTGGATTTTTCGC‑TTTTTTCCCAGCAT‑TAagaaga                                                                                                               >  1:878422/1‑138 (MQ=255)
                             gAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGC‑TTTTTGCCCAGCAT‑TCAGACGaaa                                                                                                             >  8:704555/1‑140 (MQ=255)
                             gAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTTCCCAGCAT‑TCAGACGaaa                                                                                                             >  7:987532/1‑140 (MQ=255)
                                 aGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAAt                                                                                                           <  2:293659/138‑1 (MQ=255)
                                  ggAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCCGCAT‑TCAGACCAAAATCGc                                                                                                        >  8:1103332/1‑140 (MQ=255)
                                   gAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGcc                                                                                                       <  6:1175367/140‑1 (MQ=255)
                                       cATCGCGTAAAAA‑CGCATTTGCACCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGGTGGTTTGTGTTGTTTTTTGGG‑GTTTTGCCCAGCCT‑TCAGACGGAAATAGCCCggg                                                                                                   >  5:44611/1‑140 (MQ=255)
                                         tCGCGTAAAAC‑CGCATTTGCAACTGGCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCggg                                                                                                   <  2:883764/138‑1 (MQ=255)
                                          cgcgTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGGTTTTGCGC‑TTTTTGCCAAGCAT‑TCAGACGAAAATAGCCCgggg                                                                                                  >  2:1116393/1‑137 (MQ=255)
                                          cgcgTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGa                                                                                                  >  2:900932/1‑138 (MQ=255)
                                            cgTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATAGCCCGGGAATtg                                                                                              >  7:779933/1‑140 (MQ=255)
                                              tAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGGGGATTTTGCGC‑TTTTTTGCCCGCAT‑TCAGACGAAAATCGCCCGGGAATtg                                                                                              >  1:566942/1‑138 (MQ=255)
                                              tAAAAA‑CGCATTTGCAACTGTCGGCGCTGTTCCCGTATGTTGCTAAAGATTTTAGGAAAAACGGCCGGCGGGCCTGTTTGTTTTGTCTGGATTTTGCGC‑GTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATtgtg                                                                                            <  5:146481/140‑1 (MQ=255)
                                                 aaa‑CTCTTTTGAAACTGGCGGGGATTTTCATGTATTTTGATAAAGATTTTATGAAAAACGGCCTGGGGGCGGTTTTGTTTTGTCTGTATTTTGCGC‑TTTTTGCCAAGAAT‑TCAGACTAAAATCGCCCGGTAATTGTTaaa                                                                                         <  6:849244/140‑1 (MQ=255)
                                                 aaa‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGCAAATCGCCCGGGAATTGTGaaa                                                                                         >  4:143249/1‑140 (MQ=255)
                                                  aaccgattttGCAAGTGGCGGCGCTTTTCCAGTTGGTGTCTAAAGATTTTATGAAAAACGGAGTGGGGGCCGTTTTGTTTTGTCTGTATTTTGCGA‑TTTTTGCCCAGCAT‑TCAGACGAAAACCGCCTGGTAATTGTGaaa                                                                                         <  6:123279/133‑1 (MQ=255)
                                                         tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGCATTGTGAAAAAATa                                                                                    >  2:503208/1‑138 (MQ=255)
                                                               aCTGTAGTCGCTTTTCCAGTATGTCGATAAAGATTTTATGAAAAACTGCCTGCGGGCAGTTTTTTTTTGTCTGGATTTTGCGC‑TTTTTGCCCATCAT‑TCAGACGAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAg                                                                            <  6:871272/140‑1 (MQ=255)
                                                                cTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGGTTTGTCTGGATTTTGCGC‑GTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAgc                                                                           >  8:183925/1‑140 (MQ=255)
                                                                 ggTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATCAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCACACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAgcg                                                                          <  4:384501/139‑1 (MQ=255)
                                                                  gTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGG‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAgcgc                                                                         >  4:97748/1‑140 (MQ=255)
                                                                    cGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAgcgc                                                                         >  1:841322/1‑138 (MQ=255)
                                                                     ggCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCAGGATTTTGCGC‑ATTTTGCCCAGCAT‑ACAGCCGAAAATAGCCCTGGAATTGTTCAAATAAACGCGCCAgcgcgcc                                                                      >  6:874556/1‑139 (MQ=255)
                                                                         tttttCCAGTATGTTGATAAAGATTTTATCAAAAACGGCCTGGGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                                                                  <  8:582772/139‑1 (MQ=255)
                                                                         cTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                                                                  <  6:768760/140‑1 (MQ=255)
                                                                          ttttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGC‑GTTTTGGCCAGCAT‑TGAGACGCAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAttaa                                                                 >  4:503537/1‑137 (MQ=255)
                                                                               cAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGCCGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGt                                                              >  2:1145779/1‑138 (MQ=255)
                                                                               cAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTTCGC‑TTTTTTCCCAGCAT‑TAAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGCCAGCGCGCAAAAACCGt                                                              >  1:1114793/1‑138 (MQ=255)
                                                                                  ttttttGCTAAATTTTTTATCAAAAAGGGCCTGCGGGGGTTTTTGTTTTTTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACTAAAATCCCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                                         <  5:553557/136‑1 (MQ=255)
                                                                                  tttGTTCCTATAGATTTAAGCAAAAACGCCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGCAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                                         <  8:493259/138‑1 (MQ=255)
                                                                                   ttGTTGATAAAGATTTTATTAAAACCGGCCGGCGGGCGGTTTTGTTTTGTCGGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCCCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                        <  5:1049044/139‑1 (MQ=255)
                                                                                    tGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGGCCAGCAG‑TCAGACGAAAATCTCCCGGGATTTGTGTAAAAATAACCGACAGCGCGCCATAACCGttggagac                                                       >  3:470352/1‑134 (MQ=255)
                                                                                    tGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGCCAGCGCGCAATAACCGTTCTCGAc                                                       >  6:441350/1‑140 (MQ=255)
                                                                                    tGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                                                       <  3:637188/140‑1 (MQ=255)
                                                                                        gCTAATGATTTTATGAAAAAGGGCCGGCGGGCCGTTTTGTTTTGTCTGGTTTTTGCGC‑TTTTTGCCCAGAAT‑TCAGACTAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCAt                                                   <  6:989570/140‑1 (MQ=255)
                                                                                         cTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACCGCGCGCAATAACCGTTCTCGACTCATa                                                  >  6:1053789/1‑140 (MQ=255)
                                                                                            aaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGATTTTTGGC‑TTTTTGGCCAGCAT‑TCAGACGCAAATAGCCCGGGTATTTTGAAAAAATACGCGACAGCGCGCAATAACCGTTATCGACTAATaaaa                                               >  3:419632/1‑140 (MQ=255)
                                                                                              gATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa                                               >  2:369049/1‑138 (MQ=255)
                                                                                                ttttATTAAAAACGGCCTGCGGGCCGTTTTTTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGAAT‑TCAGCCTAAAATCGCCCGGTAATTGTTAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa                                           <  4:419632/140‑1 (MQ=255)
                                                                                                ttttATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGATTTTGCGG‑TTTTTGCCCAGCAT‑TCAGCCGAAAATGGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTGTCGACGAATAAAAGTgt                                           >  4:798584/1‑139 (MQ=255)
                                                                                                 tttATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCCGCAT‑TCAGGCGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGGTCTCGCCTCATAAAAGTGAt                                          >  4:262113/1‑140 (MQ=255)
                                                                                                   tATTAAAACCGGCCTGCGGGCCGTTTTGTTTTGTCTGGTTTTTGCGG‑TTTTTGCCCAGAAT‑TCATACTAAAATCCCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  6:242654/140‑1 (MQ=255)
                                                                                                   tATTAAAAACGGCCTGCGGGCCGTTTTTTTTTGTGTGTATTTTTCGT‑TTTTTGCCCATCAT‑TAATACTAAAACCGCACGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                          <  2:157062/138‑1 (MQ=255)
                                                                                                   tATGACAACGGGCCTGGGGGCGGTTTTGTTTTGTCGGTTTTTTGCGG‑TTTTTCCCCAGCAT‑TAATCCGAAAATCGCCCGGTATTTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  5:35928/140‑1 (MQ=255)
                                                                                                   tATGAAAAAGGGCGTGCGGGCGGTTTTTTTTTGTGTGTATTTTGCGT‑TTTTTGCCCTGAAT‑TCAGACAAAAATCGCCGGGTATTTGTCAAAAAATACGCGACAGCGCGAAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  4:182783/140‑1 (MQ=255)
                                                                                                   tATGAAAAACGGCCTGGGGGCGGTTTTTTTTTGTCTGGATTTTGCGC‑TTTTTGCCCATCAT‑TCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  3:836417/140‑1 (MQ=255)
                                                                                                   tATGAAAAACGGCCTGCGGGCGGTTTTGTTTTGTGTGGATTTTGGGG‑TTTTTGCCCAGCTT‑TAAGACGAAAATCGCCGGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  3:1076853/140‑1 (MQ=255)
                                                                                                   tATGAAAAACGGCCTGCGGGCCGGTTTGGTTTGTCTGGTTTTTGCGC‑TTTTTGCCCCGCAT‑TGAGACGAAAATCGCCCGGGCATTGTGAAAAAATACGCGCCAGCGCGCAATAACCGTTCTCGCCTCATAAAAGTggt                                          >  2:1078906/1‑136 (MQ=255)
                                                                                                   tATCAAAAACGGCTTGCGGGCCGTTTTGTTTTTTGTGGATTTTGCGC‑TTTTTGCCCATATT‑TCACAATAAAATCGCCCGCGAATTTTAAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                          <  1:1091364/138‑1 (MQ=255)
                                                                                                     tGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGATTTTGCGA‑TTTTTGCCCAGCAT‑TCATACGAAAATCGCCGGGGAATTTTGAAAATATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                        <  1:715542/138‑1 (MQ=255)
                                                                                                     tGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCg                                      <  8:159406/140‑1 (MQ=255)
                                                                                                     tGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGAAT‑ACAGACGAAAAGCGCCCGGGAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCg                                      <  3:466480/140‑1 (MQ=255)
                                                                                                               ccTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc                            >  4:1125024/1‑140 (MQ=255)
                                                                                                                cTGCGGGCGGTTTTGTTTTGGCTGGATTTTGCGC‑TTTTGGCCCAGCAT‑TCACACTAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGc                             <  1:439001/138‑1 (MQ=255)
                                                                                                                cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGAAT‑TCAGACTAAAATCGCCCGGTAATTGTTAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                           <  3:972369/140‑1 (MQ=255)
                                                                                                                cTGCGGGCCGTTTTGTTTTGGCTGGATTTTGCGC‑GTTTTGCCCAGCAT‑TCAGCCGAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCTCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGc                             <  2:115506/138‑1 (MQ=255)
                                                                                                                  gcgggcggTTTTGTTTTGTCTTGTTTTTGCGA‑TTTTTGCCCAGCTT‑TCAGACTAAAATCGCCCGGGAATTGTCAAAAAATACGCGACAGCGCGCAATAACCGTGCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                         <  4:1019047/140‑1 (MQ=255)
                                                                                                                  gCGGGCTGTTTTGTTTTGTCTGGTTTTTGCGG‑TTTTTCCCCAGAAT‑ACCTACTAAAATCGCCTGGTAATTTTTAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                         <  4:209262/140‑1 (MQ=255)
                                                                                                                  gCGGGCGTTTTTTTTTTGTCTGTTTTTTGCTT‑TTTTTCCCCAGATT‑TCACACTAAAATCGCCCGGCAATTGTTAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                         <  7:704555/140‑1 (MQ=255)
                                                                                                                  gCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                           <  2:226791/138‑1 (MQ=255)
                                                                                                                    gggctttttttttttgTTGGTATTTTGGGT‑TTTTTGCCCAGAAT‑TAAGACTAAAATCGCCCGTTATTTGTTAAAAAATACGCGACAGCGCGCAATTACCGTTCTCGAATCATAAAAGTGATGCCGCTATAATGCcgcg                         <  1:272788/132‑1 (MQ=255)
                                                                                                                    gggcgttttttttttgTGTGTTTTTTGCTC‑TTTTTG‑CCACCATATCTCACTATTATCGCCCGGTAATTGTTAAAATATACGCGACTGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTc                       <  3:1026178/134‑1 (MQ=255)
                                                                                                                    gggCAGTTTTTTTTTTTCTGTATTTTCCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCCCCCGGTAATTGTTAAAAAAACCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTc                       <  7:221448/140‑1 (MQ=255)
                                                                                                                             tgttttgtCTGGATTTTGCGC‑TTTTTGCCAAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTCAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa              <  4:288596/140‑1 (MQ=255)
                                                                                                                             tgtttgttCTGGATTTGGCGC‑TTTTTCCCCTGCTT‑TCATACTAAAATCGCCCGGTATTTGTTAAAAAATACGCGACAGCGCTCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa              <  3:732516/140‑1 (MQ=255)
                                                                                                                                 ttgtTTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACTAAAATCGCCGGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCt          <  4:1009007/140‑1 (MQ=255)
                                                                                                                                   gacTGGATTTTGGGC‑TTTTTCCCAAGCAT‑TCAGACTATACTCGCCCGTTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt        <  7:113456/138‑1 (MQ=255)
                                                                                                                                    tCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCTTTCTCGACTCATAAAAGTGATGCCGCTATAATCCCGCGTCCTATTTGAATGCttta       >  5:973133/1‑139 (MQ=255)
                                                                                                                                    tCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc       >  5:839997/1‑140 (MQ=255)
                                                                                                                                    tCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc       >  3:1151285/1‑140 (MQ=255)
                                                                                                                                        taTTTTTCGC‑TTTTTCACAATCTG‑TTAGACCAAATTCGCCCGGTATTTGGTAAAAAATACGCCACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCGGCTATACTGCCGCGTCCTATTTGAATGCTTTCGGga   <  6:1077150/139‑1 (MQ=255)
                                                                                                                                        taTTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCTGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCgg     <  2:1190910/137‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                             
CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAA‑CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTTGCCCAGCAT‑TCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGAT  >  NZ_CP022686/4014106‑4014381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A