Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F13 I0 R1 230 5035.0 9125430 98.4% 8979423 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,546,394 A→C 12.6% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,546,3940AC12.6% 291.3 / 8.0 166V95G (GTT→GGT) waaGglycosyltransferase family 4 protein
Reads supporting (aligned to +/- strand):  ref base A (84/55);  new base C (2/22);  total (87/79)
Fisher's exact test for biased strand distribution p-value = 2.29e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

CAGCAGCTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAA  >  NZ_CP022686/2546255‑2546533
                                                                                                                                              |                                                                                                                                            
cagcagATGTGTCGGATTGCCCTTATCTAATGTTGCCCTATCAAAGGCGGCATAAAGGAGATAAAGTGACGTCAGGCGATAGAAAAAGCCTTTATCCTGCGCTACTTTCTCCTCATAAC‑AAA‑CTGCAGAGTC‑ATACTaaa                                                                                                                                              >  2:388466/1‑140 (MQ=255)
   cagcTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGt                                                                                                                                           >  3:441316/1‑140 (MQ=255)
    agcTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTc                                                                                                                                          <  3:65888/140‑1 (MQ=255)
       tgtgTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAg                                                                                                                                       >  1:406138/1‑140 (MQ=255)
         tgtCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGcc                                                                                                                                     >  6:443426/1‑140 (MQ=255)
          gtCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATAGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATACC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGc                                                                                                                                      >  8:127517/1‑138 (MQ=255)
           tCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCTCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGcccc                                                                                                                                   >  6:843459/1‑140 (MQ=255)
           tCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGcccc                                                                                                                                   >  6:152897/1‑140 (MQ=255)
            cGGCTTGCCCTGTTCGAAGGTTGACCGCTCATAGGCGGGATAATGGCGTTTAGGTGACGTCAGGCGATTGTTAAAGCCTTTCTCCTGCGATACTTTCTCGGCATTAC‑AAA‑CATGTGCGGC‑ATAATAAACGACCAGccc                                                                                                                                    <  8:249983/138‑1 (MQ=255)
                 tGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAAAGTGACGGCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCa                                                                                                                               <  7:4700/138‑1 (MQ=255)
                   cccTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCAtttt                                                                                                                           >  5:230540/1‑140 (MQ=255)
                        ttCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTAAGGCGCTAGAAAAAGCCTTTCTCCTGCGCAACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGAATATTAT‑Taa                                                                                                                     >  6:657790/1‑140 (MQ=255)
                         tCGAAGGGTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGGCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑Taaa                                                                                                                    <  5:757986/140‑1 (MQ=255)
                          cGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGTCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑Taa                                                                                                                     >  8:672480/1‑138 (MQ=255)
                          cGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑Taa                                                                                                                     >  8:626345/1‑138 (MQ=255)
                            aaGGTGGCCCGCTCAACGGCGTAATAATGGCCATACCGTGACGTCCGGCTATATAAAAACCCTTTCTCCTGCGTTACTTTCTCGGCAAAAC‑AAA‑AATCAGGGTA‑ATAATAAACGTCCAGCCCCGGAATTTTAT‑TAAAt                                                                                                                   <  8:287392/138‑1 (MQ=255)
                              ggTTGCCCGCTAAATGGCGGAATAATGGCTATAACGTTCCGGCCGGCTATACAAAAAGCCTTTCTCCTGCGCTAATTTCTCGCCAAAAC‑AAA‑CATCAGCGCA‑ATAAAAAACTTCCCGCCCCGGAATTTTAT‑TAAAAccca                                                                                                               <  6:237415/140‑3 (MQ=255)
                               gTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCa                                                                                                                >  7:931407/1‑138 (MQ=255)
                                 tGCCCGGTCATAGGCGGAATACTGGCGATAACTTGACGTCAGGCGTTATAAAAAGCATTGCTCCTGCGATAATTTCTCGGAATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCTGCCCCGGCTTTTTAT‑TAAAGCCAAc                                                                                                              <  8:183747/138‑1 (MQ=255)
                                 tGCCCGCTAAATGGCGTCATAATGGCTATAACGTGACGTCAGGCGATAGAAAAAGCATTTCTCCTGCGCAACTTTCTCGGCATAAC‑AAA‑CATCAGCGCA‑ATAAAAAACTTCCAGCCCCGGCATTTTAT‑TAAATCCAACGa                                                                                                            <  2:306481/140‑1 (MQ=255)
                                 ggCCCGCTCAAAGGCGACATAATGGCGATACCGTGCCGTCCGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAA‑AAA‑CATCAGCGGC‑ATAATAAACGGCCAGCCCGGGAATTTTAT‑TAAATCCAACGa                                                                                                            <  2:822441/139‑1 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGATACAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGAc                                                                                                           >  3:565519/1‑140 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGAc                                                                                                           >  2:918942/1‑140 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGAc                                                                                                           >  5:44730/1‑140 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGAGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGAc                                                                                                           >  4:300187/1‑140 (MQ=255)
                                    ccccacAACGGCGGAATAATGCCCATAACGGGACGTCTGGCGATACAAACAGCCTTGGCCCTGCGCTACTTTCTCGGAATAAC‑AAA‑CAGCCGCGTA‑ATAAAAAACGCCCACCCCGGGATTTTTAT‑TAAATCCAACGActc                                                                                                         <  2:127294/135‑1 (MQ=255)
                                       cTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTAt                                                                                                      >  5:45158/1‑140 (MQ=255)
                                          aaaGGGGGCATAAGGGCGAAACCGTCACGTCAGGCGATACAAAAAGCCTTTCTCCTGCGCTACTTTCGCGCAATAAC‑AAA‑CATC‑CCGGCAATAATAAACGCCCAGCCCCGGCATTTTAT‑TAAACCCCACGACTCTATc                                                                                                     <  8:922059/138‑1 (MQ=255)
                                          aaaGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTGTCTCGGCATCAC‑AAA‑CATCAGCGGC‑ATAATAACCGTCCCGCCCCGGCATTTGAT‑TACATCCATCGACTCTAACgc                                                                                                   >  6:1117411/1‑139 (MQ=255)
                                          aaaGCCGGAATAATGGCGATACCGTCACGACCGGCGAATTAAAACGCCTTTACCCTGCGCTACATGCTCGGCTTCAC‑AAA‑CCCCACCGGC‑ATAATAAACGTCCAGCCCGGGCTGTTTAT‑TAAATCCAACGACTCTATc                                                                                                     <  8:350073/138‑1 (MQ=255)
                                           aaGGTGTCATAATTGCGATAACGTTACGTAAGGCGATAGAAAAAGCCTTTCACCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCTGC‑ATAATAAACGGCCAGCCCCGGCATTTTAT‑TAAAACCAACGACTCTATCGAc                                                                                                  <  6:393309/140‑1 (MQ=255)
                                              gcggcATAAGGGCGATAACGTCCCGGCCGGCGATACAAAAAGCCTTTCGCCTGCGCTACTTTCGCGGCATAAC‑AAC‑AATCTGCGCC‑AAAATAAACGTCCCGCCCCGGAATTTATT‑TAAAACCAACGACTCTATCGACg                                                                                                 <  8:251784/138‑1 (MQ=255)
                                                 gcATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGgtg                                                                                            >  4:806463/1‑140 (MQ=255)
                                                 gcATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGgtg                                                                                            >  6:339161/1‑140 (MQ=255)
                                                 gcATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑GAAATCCAACGCCTCTATCGACGGGgtg                                                                                            >  4:801262/1‑140 (MQ=255)
                                                  cATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGgtgt                                                                                           >  6:108942/1‑140 (MQ=255)
                                                    tAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTATCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCTACGGGgttt                                                                                           >  7:273837/1‑136 (MQ=255)
                                                        ggCGATAACGTGACGTCAGGCGATAGAAAAAGCATTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACgg                                                                                     >  3:877042/1‑139 (MQ=255)
                                                            aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAATTCCAACGACTCTATCGACGGGGTGTTCCCGTAAAt                                                                                 >  1:826261/1‑140 (MQ=255)
                                                            aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTaaaa                                                                                 >  3:1149310/1‑139 (MQ=255)
                                                            aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                 >  1:136831/1‑140 (MQ=255)
                                                            aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                 >  3:543398/1‑140 (MQ=255)
                                                            aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑AAAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                 >  1:426095/1‑140 (MQ=255)
                                                              aaCGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                 >  7:1008511/1‑138 (MQ=255)
                                                              aaCGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                 >  7:98475/1‑138 (MQ=255)
                                                              aaCGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCAGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                 >  7:456806/1‑138 (MQ=255)
                                                                    aCGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGc                                                                         >  6:902753/1‑140 (MQ=255)
                                                                       acAGGCGAAAGAAAACCCCTTACACCTGCGCAACTTTCCCGCCATAAC‑ACA‑ACTCCGCGGA‑ATAATACACGCCCCCCCCCGGCATTTTAT‑AAAAACCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCAcc                                                                      <  5:915884/139‑1 (MQ=255)
                                                                              aTAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCTGCGGC‑ATAATAATCGTCGAGCCCCGGCTTTTTTT‑TAAATCCAACGACTCTATCGAAGGGGTGTTCACGTTATTGTTTGTGCACCCAGGCaa                                                               >  4:686759/1‑140 (MQ=255)
                                                                              aTAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCCGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCCCCCAGGCaa                                                               >  4:605519/1‑140 (MQ=255)
                                                                               tAGAAAACGCCTTTCTCCTGCGCTACTTTCTCGGAATAAC‑AAA‑CATCCCCGGC‑ATAATAACCGCCCAGCCCCGGCATTTTAT‑TAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCaaa                                                              <  6:280709/140‑1 (MQ=255)
                                                                               tAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAATTGTTTTTGCACCCAGGCaaa                                                              >  2:1122210/1‑140 (MQ=255)
                                                                                aGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCGCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCaaaa                                                             >  2:1053024/1‑140 (MQ=255)
                                                                                 taaaaccccTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGCC‑ATAAAAAACGTCCCCCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAat                                                            <  2:1108588/133‑1 (MQ=255)
                                                                                     atcccTTTCTCCTGCGCAACTTTCCCGGAATAAA‑AAA‑CATCACCGCC‑ATAATACACGCCCAGCCCGGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAatat                                                          <  8:532641/135‑1 (MQ=255)
                                                                                     aaGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑AGAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTc                                                        >  6:796435/1‑140 (MQ=255)
                                                                                         cttccaccTGCCCTACTTTCGC‑GCAAAAC‑AAACCACCCCCGCA‑AAAAAAAACGCCCCCCCCCGCCATTTTAT‑AAAAACCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCg                                                      <  7:33584/132‑1 (MQ=255)
                                                                                         atttCACCTGCGCAACTTTCTCGG‑ACAACAAAA‑CATCCGCGGC‑ATAATACCCGTCCCCCCCCGGCATTTTAT‑TAAAACACACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCa                                                    <  4:309154/139‑1 (MQ=255)
                                                                                           ttCACCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTAACGTAAATGATTTTGCACCAAGGCAAAATATTCCGCAtt                                                  >  3:304767/1‑140 (MQ=255)
                                                                                            tctcCTGCGCTACTATCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTATTGCACCCAGGCAAAATATTCCGCATTg                                                 >  1:1072163/1‑140 (MQ=255)
                                                                                              cccggcgcTAATTTGTCGCAAAAAA‑AAA‑CATCCGCGCA‑AAAAAACACGCCCACCCCCGGCTTTTTTT‑AAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTgcg                                               <  2:426095/136‑1 (MQ=255)
                                                                                              cccTGCGCTATTTTCTCGGAATAAC‑AAA‑CATCCGCTCA‑ATAATAACCGTCCCGCCCCGGAATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTgcg                                               <  3:245922/139‑1 (MQ=255)
                                                                                                  gcgcTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCAAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCg                                           >  4:429244/1‑140 (MQ=255)
                                                                                                   cgcTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGt                                          >  1:1100056/1‑140 (MQ=255)
                                                                                                     ctcatttCTCGGAATAAA‑AAA‑CATCAGCGTC‑ATACTAAACGTCCCGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGt                                          <  8:993817/134‑1 (MQ=255)
                                                                                                      taatttCTCGGCATAAC‑AAA‑CATCAGCGTA‑ATAATAAACGTCCCGCCCCGGATTTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTg                                         <  8:851291/135‑1 (MQ=255)
                                                                                                      tACTTTCTCGGCATAAC‑AAA‑CTTCAGCGGC‑ATAATAAACGACCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTg                                         <  8:547736/138‑1 (MQ=255)
                                                                                                      aaCTGTCTCGGAATAAC‑AAC‑AGTCTTCGGA‑ATAAAAAAAGTCACGCCCGGTCATTTTAT‑TAATTCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGAt                                       <  3:699950/139‑1 (MQ=255)
                                                                                                        cTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTg                                     >  3:450789/1‑140 (MQ=255)
                                                                                                         tttCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTgg                                    >  5:1003294/1‑140 (MQ=255)
                                                                                                                  aTAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCACATTGCGCCCGTGATTGGTATGCGAtt                           >  5:570802/1‑140 (MQ=255)
                                                                                                                   aaac‑aaa‑caTCCGCGGA‑AAAATAAACGTCCCCCCCCGCAATTTTAT‑TAAAACCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGAttt                          <  3:76001/139‑1 (MQ=255)
                                                                                                                    aac‑aaa‑caTCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGATTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTa                         >  6:583246/1‑140 (MQ=255)
                                                                                                                        aaaccctCAGCGCA‑ATAATAACCGGCC‑CCCCCCGCATTTTTTATAAAACCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAAc                       <  2:468844/134‑1 (MQ=255)
                                                                                                                         aa‑caTCCGCGGC‑ATAATAAACGGCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                     <  4:441316/140‑1 (MQ=255)
                                                                                                                         aa‑caTCAGCGGC‑ATAATAACCGTCCACCCCCGGCATTTTAT‑TAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                     <  2:61878/140‑1 (MQ=255)
                                                                                                                         aa‑caTAAGCTTC‑ATAATAACAGTCAAGACCAGGATTTTTAT‑TAAAACAAACGACTCTCTCGACGGGGTGTTCACGTAAATGTTTTTGCAGCCAGGCAAAATATTCCTCATTGCGACCGTGATTGGTATTCGATTTAAcct                     <  4:999030/140‑2 (MQ=255)
                                                                                                                         aa‑caCCAGCGCA‑AAAATAACCGTCCAGCCCCGCCATTTAAT‑AAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                     <  4:388285/140‑1 (MQ=255)
                                                                                                                          acaaTCAGCGGC‑ATAATAACCGTCCAGCCCCGGCATTTTAT‑TAAATCAAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                     <  1:950883/137‑1 (MQ=255)
                                                                                                                          aaaaTCCGCGGA‑ATAATAACCGTCCAGCCCCGTCTTTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                     <  3:1102148/137‑1 (MQ=255)
                                                                                                                             aTCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCa                  >  1:218031/1‑140 (MQ=255)
                                                                                                                               cAGCGGA‑ATAATAAACGACCAGCCCCGGCATTTAAT‑TAAATCCAACGACTCTATCGACTGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCAGTGATTGGTATGCGATTTAACCGGCACt                <  4:245892/140‑1 (MQ=255)
                                                                                                                                aGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACtt               >  1:634805/1‑140 (MQ=255)
                                                                                                                                  cGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTg             >  2:718570/1‑140 (MQ=255)
                                                                                                                                  cGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCAAACGACTCTATCGACGGGGTTTTAACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGAATGGTATGCGATTTAACCGGCACtatt             >  2:340004/1‑137 (MQ=255)
                                                                                                                                    gC‑ATAATAAACGTCCAGCCACGGCATTTTAA‑TAAATCCAACGACTCTATCGACGTGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCCCTATg             >  7:190570/1‑138 (MQ=255)
                                                                                                                                    cc‑ATAATAAACGTCCACCCCCGGCTTTTTAT‑TAAAACCAACGACGCTATCGTCGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGACCGTGATTGGTATGCGATTTAACCGGCACTTTGAt           <  6:867394/139‑1 (MQ=255)
                                                                                                                                     gaataataAACGACCCCCCCCGGAATTTTAT‑AAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGAt           <  5:108942/138‑1 (MQ=255)
                                                                                                                                     c‑ATAATAAACGTCCAGCCCCTGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTAGGCGATTTAAACGGCACTTTGagc          >  5:832369/1‑138 (MQ=255)
                                                                                                                                     c‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc          >  6:1018049/1‑140 (MQ=255)
                                                                                                                                      cttaataaAAGTACCGCCCCGGCATTTTTT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGAACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc          <  6:72744/138‑1 (MQ=255)
                                                                                                                                      aataataAACTTCAAGCCCCGTCTTTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc          <  1:299561/139‑1 (MQ=255)
                                                                                                                                      aataataAACGTCCCGCCCCGGAATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc          <  1:228223/139‑1 (MQ=255)
                                                                                                                                      aaaaataaCCGGCCACCCCGGGCATTTTAT‑AAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc          <  3:16522/137‑1 (MQ=255)
                                                                                                                                         aataaCCGCCCAGCCCCGGAATTTTAT‑TAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGt       <  2:697376/140‑1 (MQ=255)
                                                                                                                                         aaaaaaCGACCACCCCCGTATTTTTTT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGt       <  5:843459/137‑1 (MQ=255)
                                                                                                                                          ataaACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTACCCGGCACTTTGATCAg        >  8:113792/1‑138 (MQ=255)
                                                                                                                                             aaCGGCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaa   <  6:867499/140‑1 (MQ=255)
                                                                                                                                              aCGTCCACCCCCGGCTTTTTAT‑TAAATCCAACGACTCTATCGACGGTGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaaa  <  2:221017/140‑1 (MQ=255)
                                                                                                                                              aCGCCCTGCCCCGTAATTTTAT‑AAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaaa  <  1:918942/140‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                            
CAGCAGCTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAC‑AAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAA  >  NZ_CP022686/2546255‑2546533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A