Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I0 R1 235 6960.4 9135780 98.4% 8989607 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,546,394 A→C 13.8% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,546,3940AC13.8% 288.7 / 15.4 166V95G (GTT→GGT) waaGglycosyltransferase family 4 protein
Reads supporting (aligned to +/- strand):  ref base A (83/57);  new base C (2/24);  total (85/81)
Fisher's exact test for biased strand distribution p-value = 6.34e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

CAGCAGCTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAA  >  NZ_CP022686/2546255‑2546533
                                                                                                                                            |                                                                                                                                           
cagcagCTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATaaa                                                                                                                                             >  7:588490/1‑140 (MQ=255)
     gcTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGt                                                                                                                                          >  3:310580/1‑138 (MQ=255)
      cTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTccc                                                                                                                                       >  6:668044/1‑139 (MQ=255)
      cTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCa                                                                                                                                       >  2:1035587/1‑140 (MQ=255)
          gtCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGccc                                                                                                                                   <  6:85236/140‑1 (MQ=255)
            cGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGAGTTTACGTGACGTCAGTCGATAGAATATGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAAAATCAGCGGCATAATAAACGTCCAGCCCCg                                                                                                                                 <  1:373878/140‑1 (MQ=255)
                 tGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtt                                                                                                                            >  6:1101826/1‑140 (MQ=255)
                 tGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtt                                                                                                                            <  6:48335/140‑1 (MQ=255)
                 tGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtt                                                                                                                            <  5:1043625/140‑1 (MQ=255)
                 tGCCCTGTGCGAAGGTTGCCCGCTCAAAGGCGGCATTATGGCGATAACGTGACGGCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtt                                                                                                                            <  1:537502/140‑1 (MQ=255)
                   cccTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtttt                                                                                                                          >  8:800266/1‑140 (MQ=255)
                   cccTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtttt                                                                                                                          >  7:350018/1‑140 (MQ=255)
                   cccTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtttt                                                                                                                          >  8:15956/1‑140 (MQ=255)
                   cccTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAAAAAACGTCCAGCCCCGGCAtttt                                                                                                                          >  6:532588/1‑140 (MQ=255)
                     cTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtttt                                                                                                                          >  3:1037810/1‑138 (MQ=255)
                     cTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtttt                                                                                                                          >  3:879213/1‑138 (MQ=255)
                       gTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTttatta                                                                                                                      <  1:628311/140‑1 (MQ=255)
                       gTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTttatta                                                                                                                      <  1:110875/140‑1 (MQ=255)
                         tCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTaaa                                                                                                                    <  7:800266/140‑1 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCTATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCGCCGGCATTTAATTAAATCCAACGAc                                                                                                           >  8:1088148/1‑140 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGAc                                                                                                           >  6:580212/1‑140 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGAc                                                                                                           >  5:802186/1‑140 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGAc                                                                                                           >  7:125254/1‑140 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGAc                                                                                                           >  5:1003638/1‑140 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGAc                                                                                                           >  5:688209/1‑140 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGAc                                                                                                           >  5:571620/1‑140 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGAc                                                                                                           >  7:967023/1‑140 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGAc                                                                                                           >  8:150219/1‑140 (MQ=255)
                                  gCCCGCTCAAAGGCGGCATAATGGCGATAAAGTGACGTCAGGCGATAGAAAAAGCCGTTCTCCTGCTCAACTTTCTCGGCAA‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTAATTAACTCCAActaa                                                                                                           >  1:862370/1‑137 (MQ=255)
                                    ccGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTGCTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCCGCCCCGGCATTTTATTAAATCCAACGAc                                                                                                           >  3:1022243/1‑138 (MQ=255)
                                    ccGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGAc                                                                                                           >  3:1103894/1‑138 (MQ=255)
                                    ccGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGAc                                                                                                           >  3:530523/1‑138 (MQ=255)
                                    ccGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGAc                                                                                                           >  4:513507/1‑138 (MQ=255)
                                            tggcggcATATTGGAGATACCGTGCCGACTGGCTATTTAAAAAGCGTTTCTCTTGCGCTAATTTTTCGGAAT‑AACAAACATCCCCGGAATAATAAACGTCCCGCCCCGGCATTTTATTAAATCCAACGACTCTATCGa                                                                                                   <  4:593835/137‑1 (MQ=255)
                                              gcggaATAATGGCGATAACGTGACGTCAGGCTATAGAAAAAGCCTTTCTCCTGCGCTACTTTCGCGGCAA‑AACAAACATCAGCGGCAAAATAACCGTCCCGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACggg                                                                                               <  2:1125839/140‑1 (MQ=255)
                                              cccgcATAATGGCGATACGGTTAGCACAGGCGAAACAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAAAAACCGCGCAATAATAACCCACCACCCCGGGAATTTAATTAAAACCAACGACTCTATCGACggg                                                                                               <  8:269321/137‑1 (MQ=255)
                                                 gcATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGgtg                                                                                            >  6:879567/1‑140 (MQ=255)
                                                 gcATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCAGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACTACTCTCTCGAAGGGgtg                                                                                            >  6:459676/1‑140 (MQ=255)
                                                  cATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGgtgt                                                                                           >  7:411922/1‑140 (MQ=255)
                                                  cATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCGTTCTACTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACTTCCCGCCCAGGCATTTTCTTAAATCCAACTACTCTATCGCCGGGgttt                                                                                           >  8:936589/1‑138 (MQ=255)
                                                    tAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATACACGGCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGgtgt                                                                                           >  4:887204/1‑138 (MQ=255)
                                                    tAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGgtgt                                                                                           >  4:1105823/1‑138 (MQ=255)
                                                            aTAACGTGACGTCAGGCGATACAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATAAGCGGCATAATAAACGTCAAGCCCCGGCATTTTATTAAATCCAACGCCTCTATCGACGGGGTGTTCACGTAAAt                                                                                 >  8:541058/1‑140 (MQ=255)
                                                                  tGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCAGAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGttt                                                                             >  3:821240/1‑138 (MQ=255)
                                                                    aCGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGc                                                                         >  1:1043206/1‑140 (MQ=255)
                                                                       tccggCTATACAAAAAGCCTTTCTCGTGCGCTACTTTCTCGGCAT‑AACAAACATCCGCGGCATAATAACCGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCAcc                                                                      <  2:36568/137‑1 (MQ=255)
                                                                           gCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACACACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATGAAATCCAACGACTCTATCGACGGGGTGTTCCCGTAATTGTTTTTGCACCCAgg                                                                  >  8:115621/1‑140 (MQ=255)
                                                                           gCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAgg                                                                  >  7:56602/1‑140 (MQ=255)
                                                                           gCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGATCACGTAAATGTTTTTGCACCCa                                                                    >  4:82103/1‑138 (MQ=255)
                                                                             catatAAAACGCCTTCCCCCTGCGCGAATTTCTCGGCAT‑AACAACCATCCGCGGCAAAAAAAACGACCCCCCCCGTCATTTTATTAAAACCCACGACTCTATCGACGTGGTGTTCACGTAAATGTTTTTGCACCCAGGCa                                                                <  6:194910/139‑1 (MQ=255)
                                                                                         cTTTCTCCTGCGTTACTTTCTCGGCAT‑AAAAAACATCTGGGGAATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCa                                                    <  6:599826/140‑1 (MQ=255)
                                                                                         atttCCCCTGCGAAATTTTCTCGGCAA‑AACAACCAACAGCGGAAAAATAAACTTCCCCCCCCGGAATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCg                                                      <  3:137414/137‑1 (MQ=255)
                                                                                           ttCCCCTCCGCAACTTTCGCGCCAA‑ACCAAACACCCCCGGCATAAAAACCGTCCCGCCCCGCCATTTTATAAAAACCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCAtt                                                  <  2:398725/140‑1 (MQ=255)
                                                                                            tctcCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTg                                                 >  1:333041/1‑140 (MQ=255)
                                                                                            tctcCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCACGGCATTTTATCAAATACAACGACTCTATCGACGGTGTGTTGACGTAAATGTTTTTGCACCCAGGCAATATATTCCGCATTg                                                 >  5:706653/1‑140 (MQ=255)
                                                                                              tcCTGCGATAATTTCTC‑GCATAAACAAACTCCCGCGTAATAAAAAACGCCCAGCCCCGGATTTTTATAAAATCACACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTgcg                                               <  1:1027989/140‑1 (MQ=255)
                                                                                                      taatttCTGGGCAT‑AACAAACATCAGCGCCATAATAAAAGCCCAGCCCCGGAATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTg                                         <  3:906665/135‑1 (MQ=255)
                                                                                                      taatttCTAGGCAC‑AACAAACAGAACCTGCAAAAAAACAGTCCACCCCAGCAATTTAATAAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACACAGGCAAAATATTCCGCATTGCGCCCGTg                                         <  4:310580/135‑1 (MQ=255)
                                                                                                      tACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCACCCCCGCCATTTTATTAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTg                                         <  4:731311/138‑1 (MQ=255)
                                                                                                        cTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTTCACCACGGCAAAATATTCCGCATTGCTCCCGTGATTg                                     >  7:258496/1‑140 (MQ=255)
                                                                                                           tatcGTCAA‑AACAAACATCCGCGTCATAATAAACTTCCCGCCCCGGCATTTTTTTAAAACCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTa                                  <  1:604358/138‑1 (MQ=255)
                                                                                                            ctcGGAAA‑AACAAACATCAGCGGCAAAATAAACGTCCAGCCCCGCCATTTTATTAAAACCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTAt                                 <  1:1035587/140‑1 (MQ=255)
                                                                                                             gcGGCAT‑CACAAACATCCGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTa                                  <  4:30830/137‑1 (MQ=255)
                                                                                                               ggCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATg                                >  4:992647/1‑138 (MQ=255)
                                                                                                               ggCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATg                                >  4:613101/1‑138 (MQ=255)
                                                                                                                  aT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGAtt                           >  1:1168879/1‑140 (MQ=255)
                                                                                                                     aacaaacaTCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACg                                                                                                 >  1:311818/1‑68 (MQ=255)
                                                                                                                       aaaacaTAAGCGGCATAATAACCGTCCATCCCCGGAATTTAATTAAATCCAACGACTCTATCGACGGGGGGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATCGCGCCCGTGATTGGTATGCGATTTAAc                       <  8:706099/139‑1 (MQ=255)
                                                                                                                         aacaTCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAAc                       <  4:879213/138‑1 (MQ=255)
                                                                                                                         aacaTCAGCGGCAAAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAAc                       <  4:497918/138‑1 (MQ=255)
                                                                                                                         aacaTCAGCGGAATAATAACCGTCCTGCCCCGTATTTTTTTTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAAc                       <  3:590601/138‑1 (MQ=255)
                                                                                                                          acaTCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCgg                    >  8:211932/1‑140 (MQ=255)
                                                                                                                            aTCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCAACGTGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGAGTGGTATGCGATTTACCCGGCa                  >  1:34918/1‑140 (MQ=255)
                                                                                                                            aTCAGCCGCATAATAAAAGCCCAGCCCCGCAATTTTATTAATACCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCa                  <  2:738932/140‑1 (MQ=255)
                                                                                                                              cAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGcc                  >  3:286810/1‑137 (MQ=255)
                                                                                                                               aGCTGCATAATAAACGTCCAGCCCCGGCATTTTAATAAAACCAACGACTCTATCGACGGAGTGTTAACGTAAATGTTTTTGAACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACtt               >  8:354517/1‑140 (MQ=255)
                                                                                                                                 ggtaataataAACGACCAGCCGTGTAATTTTATTAAAACACACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTg             <  1:707484/136‑1 (MQ=255)
                                                                                                                                 cGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTACGCATTGCGCCCGTGATTGGTATGCTATTTAACAGGCACTTTg             >  8:458743/1‑140 (MQ=255)
                                                                                                                                   gaataaaaAACGGCCTGCCCCGACTTTTTAATAACACCACCGACTCTATCGACTGTGGGTTCAAGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGAGATTTAACCGGCACTTTGAt           <  8:1032296/138‑1 (MQ=255)
                                                                                                                                   ccaaaaaaaaCGTCCTGCCCGGGCTTTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGAt           <  1:790182/133‑1 (MQ=255)
                                                                                                                                   ccATAATAAACGTCCAGCCCGGGCATTTTATAAAATCCCACGACTCTATCGACGGGGTGTTCACGTAATTGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGAt           <  5:668044/139‑1 (MQ=255)
                                                                                                                                   caaaaataaACGCCCCGCCCCGCCATTTAATTAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGAt           <  6:364538/136‑1 (MQ=255)
                                                                                                                                    cATAATAACCGTCCAGCCCCGGCATTTTATTAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc          <  8:116034/140‑1 (MQ=255)
                                                                                                                                    cATAATAAACGTCCCGCCCCGTAATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGa            <  3:982672/138‑1 (MQ=255)
                                                                                                                                    cATAATAAACGTCCAGCCCCGGCTTTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGa            <  4:162141/138‑1 (MQ=255)
                                                                                                                                    cATAAAAACCGCACAGCCCCGTCAGTTTATTAAAACCAACGCCTCTCTCGACGTGGTGTTCACGTAAATGTTTTAGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGTCACTTTGATc          <  6:724692/140‑1 (MQ=255)
                                                                                                                                    aataataACCGTCCCGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGa            <  3:513507/137‑1 (MQ=255)
                                                                                                                                    aataataACCGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGa            <  3:730826/137‑1 (MQ=255)
                                                                                                                                    aataataACCGTCCAGCCCCGCCATTTTATTAAATCCCACGACGCTCTCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCTATTTAACCGGCACTTTGa            <  4:699365/137‑1 (MQ=255)
                                                                                                                                    aataaaaAACGCCCCGCCCCGCCATTTTATTAAATCCCACGACTCTATCGACGGGGTGGTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGa            <  3:270569/137‑1 (MQ=255)
                                                                                                                                    aaaaataaCCGTCCACCCCCGTCATTTAATTAAATCCAACGACTCTATCGACGGGGTGTTCAAGTAAATGTTTTTGCACCCAGTCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc          <  7:773942/137‑1 (MQ=255)
                                                                                                                                     ataataAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATcc         >  1:1084301/1‑139 (MQ=255)
                                                                                                                                      taaaaaACGTCCAGCCCCGGCAATTTATTAAATCCAAAGACTCTATAGACGGGGTGTTAACGTAAATGTTATTGCACACATGCAAAATATTCCGCATGGCGCCCATGATTGGTATGAGATTTAACCGGCACTTTGATCAg        >  8:613920/1‑140 (MQ=255)
                                                                                                                                       aatacACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGt       <  7:245169/140‑1 (MQ=255)
                                                                                                                                       aataaCCGTCCAGCCCCGGATTTTTATAAATTCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGt       <  2:202058/140‑1 (MQ=255)
                                                                                                                                       aataaCCGGCCAGCCCCGGCTTTTTATTAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCa         <  4:181204/138‑1 (MQ=255)
                                                                                                                                       aataaACGTCCAGCCCCGGAATTTTATTAAACCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGt       <  1:569241/140‑1 (MQ=255)
                                                                                                                                       aataaACGCCCACCCCCGCCATTTTATTAAATCCCACGACTCTATCGACGGGGTGGTCACGTAAATGTTTTTGCACCCAGACAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCa         <  4:912618/138‑1 (MQ=255)
                                                                                                                                           aaCGTCCAGCCCGGGAATTTTATAAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaa   <  7:246288/140‑1 (MQ=255)
                                                                                                                                            aCGTCCACCCCCGTCATTTTATTAAATCCAACGACTCTATCGACGGGTTGTGCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCa    <  4:346366/138‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CAGCAGCTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAT‑AACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAA  >  NZ_CP022686/2546255‑2546533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A