Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F35 I0 R1	195	10216.4	8585726	98.3%	8439768	135.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	2,546,394	A→C	16.0%	V95G (GTT→GGT)	waaG ←	glycosyltransferase family 4 protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	2,546,394	0	A	C	16.0%	197.3 / 7.3	123	V95G (GTT→GGT)	waaG	glycosyltransferase family 4 protein
Reads supporting (aligned to +/- strand): ref base A (57/43); new base C (3/20); total (60/63)
Fisher's exact test for biased strand distribution p-value = 1.38e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

CAGCAGCTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAA  >  NZ_CP022686/2546255‑2546532

cagcagCTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATaaa                                                                                                                                             >  4:644397/1‑140 (MQ=255)

   cagcTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACACACATCAGCGGCATAATAACCgg                                                                                                                                          >  1:83311/1‑139 (MQ=255)

   cagcTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATACACGt                                                                                                                                          >  8:920733/1‑140 (MQ=255)

   cagcTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAACCGt                                                                                                                                          >  1:968891/1‑140 (MQ=255)

   cagcTGTGTCGGCTTGCCCTGTTCGAAGGTTGCACGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAATGCCTTTCTCCTGCGCTACTTTCTCAGCATAACCAACTTCAGCGGCATAATAAACgg                                                                                                                                          >  2:1034443/1‑139 (MQ=255)

    agcGGTGTCGGCTTGCCCGTGTCGAAGTTTGCCCGATCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGCCATAATAAACg                                                                                                                                           <  5:79336/138‑1 (MQ=255)

     gcTGTGTCGGCTTGCCCTGTTCGAATGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTcc                                                                                                                                        <  3:723949/140‑1 (MQ=255)

     gcTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAACCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTcc                                                                                                                                        <  1:876597/140‑1 (MQ=255)

       tgtgTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTcc                                                                                                                                        <  6:276253/138‑1 (MQ=255)

         tgtCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGcc                                                                                                                                    >  3:206389/1‑140 (MQ=255)

          gtCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTTACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGc                                                                                                                                     <  5:441056/138‑1 (MQ=255)

          gtCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGccc                                                                                                                                   <  4:287921/140‑1 (MQ=255)

           tCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACGTTCTCGGCATAACCAACATCAGCGGCATAATAAACGTCCAGcc                                                                                                                                    >  5:516283/1‑138 (MQ=255)

               cTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCa                                                                                                                              <  4:156474/140‑1 (MQ=255)

                ttGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGc                                                                                                                               >  5:452330/1‑138 (MQ=255)

                 tGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtt                                                                                                                            <  4:1057043/140‑1 (MQ=255)

                   cccTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAAAGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtttt                                                                                                                          >  4:862480/1‑140 (MQ=255)

                    ccTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAttt                                                                                                                           <  6:1021130/138‑1 (MQ=255)

                       gTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTttatt‑a                                                                                                                     <  8:349998/140‑1 (MQ=255)

                       gTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTttat                                                                                                                        <  5:557265/138‑1 (MQ=255)

                        ttCGAAGGTTGCCCGCTCAAAGGCGGCATAATGTCGATAACGTGACGACAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTGTCGGCATAACAACCATCAGCGGCATAATACACGTCCAGACCCGGCATGTTATG‑aa                                                                                                                    >  8:677946/1‑140 (MQ=255)

                        ttCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑aa                                                                                                                    >  2:1068435/1‑140 (MQ=255)

                        ttCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGAGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑aa                                                                                                                    >  2:762575/1‑140 (MQ=255)

                          cGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGAATTTTATT‑aa                                                                                                                    >  6:778570/1‑138 (MQ=255)

                               gTTGCCCGCTCAACGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCa                                                                                                               >  6:464705/1‑138 (MQ=255)

                               gTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTttatt‑a                                                                                                                     <  6:909887/132‑1 (MQ=255)

                               gTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTttatt‑a                                                                                                                     >  5:909887/1‑132 (MQ=255)

                                 ggCCCGCTCAAAGGCTGCATAATGGCGATAACGTGACGTCAGGCTATACAAAAAGCCTTCCTCCGGCGCTACTTTCTCGGCATAACAAACATCCGCGGCATAATAAACGGCCAGCCCCGGCTTTTTATT‑AAATCCAACGa                                                                                                           <  8:56036/139‑1 (MQ=255)

                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAACCATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGAc                                                                                                          >  1:1011721/1‑140 (MQ=255)

                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGAc                                                                                                          >  1:194364/1‑140 (MQ=255)

                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGAc                                                                                                          >  7:454251/1‑140 (MQ=255)

                                  gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGAc                                                                                                          >  1:610261/1‑140 (MQ=255)

                                   cccTCTCAATGGCTGAATATTGGCTATAACGTGACGTCTGGTTATAGAAAAAGCCTTTCTCCGGCGATAATTTCTCGGCATAACAAACATCAGCGGAATAATAAACTTCCCGCCCCGGCTTTTTTTT‑AAATCCAACGAct                                                                                                         <  4:79425/140‑1 (MQ=255)

                                    ccGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGAc                                                                                                          >  6:579578/1‑138 (MQ=255)

                                    ccGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGAc                                                                                                          >  6:799621/1‑138 (MQ=255)

                                       cTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTaa                                                                                                     >  4:327160/1‑139 (MQ=255)

                                          aaaGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCACCGACTCTATCGa                                                                                                  >  3:1068728/1‑140 (MQ=255)

                                            tggcggaATAAGGGCGATAACGTGACGTAAGGCTATATAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGAATAACAAACATCCGCGCAATAAAAAACGCCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACg                                                                                                <  8:635839/139‑1 (MQ=255)

                                            aGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACg                                                                                                >  3:123722/1‑140 (MQ=255)

                                              gcggcATACTGGCTAAACGGTCAGGTCCGGCGATATAAAAACCATTTCTCCTGCCCTACTTTCTCGGCATAACAAACATCAGCGCCATAATAAACGGCCCGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACggg                                                                                              <  8:295102/140‑1 (MQ=255)

                                              gcggcATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACg                                                                                                >  5:718741/1‑138 (MQ=255)

                                                  cATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGTCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGCCGGGgtgt                                                                                          >  7:793290/1‑140 (MQ=255)

                                                  cATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGCCTCTATCGACGGGgtgt                                                                                          >  4:929584/1‑140 (MQ=255)

                                                  cATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGgtgt                                                                                          >  8:920226/1‑140 (MQ=255)

                                                            aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCGTAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCCACGCCTCTATCGACGGTGTGTTCACGTaa                                                                                  >  6:254083/1‑138 (MQ=255)

                                                            aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCTACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                >  3:579662/1‑140 (MQ=255)

                                                            aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                >  1:545610/1‑140 (MQ=255)

                                                            aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTAATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                >  8:776348/1‑140 (MQ=255)

                                                              aaCGTGACGTCAGGCGATAGAAAAAGCAGTTCTCATGCGCTACTTTCTCGGCATAACAAACATCAGATGCATAATAAACGTCCAGCCACGGCATTTTATT‑AAATCCAACGACTAGATCGACTGTGTGTTAACGTAAAt                                                                                >  6:519331/1‑138 (MQ=255)

                                                                   gACGTCAGGCGATAGAAAAAGCCTTTCTCCTGAGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCACCGCCTCTATCGCCGGGGTGTTCACGTAAATGTTTTTg                                                                         >  3:477227/1‑140 (MQ=255)

                                                                       tCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCTGCATTTTATT‑AAATCCAATGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCa                                                                       <  6:147843/138‑1 (MQ=255)

                                                                          ggCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAg                                                                  >  2:238022/1‑140 (MQ=255)

                                                                           gctaacgAAAACCCCTTACTCATGCGCTACTTCCTCGGAATAAAAAACACCAGCGGCAAAATAAACGTCCAGCCCCGGCATTTCATT‑AAACCCAACGACTCTATCGACGGGGTGCTCACGTAAATGTTTTTGCACCCAgg                                                                 <  8:143912/134‑1 (MQ=255)

                                                                             taaacaaaaaCCCTTTCGCCTGCGCAAATTGCGCCCCAAAACAAACAACCCCCCAAAAAAAACCGCCCCCCCCCGGCATTTAATT‑AAACCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAgg                                                                 <  5:897579/133‑1 (MQ=255)

                                                                             aaaaaaaaaaCCCTTTCACCTGAGACAATTTCCCGCCAAAACAAACAACAGCGTCATAAAACACGTCACCCCCCGGAATTATATA‑AAAACCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCa                                                               <  7:725365/135‑1 (MQ=255)

                                                                               tAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCTACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCaaa                                                             <  4:579662/140‑1 (MQ=255)

                                                                               tAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCaaa                                                             >  4:73111/1‑140 (MQ=255)

                                                                               tAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCAAAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACAGGGTGTTCACGAAAATGATTTTGAACCCAGGCaaa                                                             >  4:152432/1‑140 (MQ=255)

                                                                               aaaaaaaaGCCTTTCCCCTGCGCTACTTACGCGCAAAAACAACCACCAGCGCAAAAAAAACCGGCCCGCCCCGCCATTTTATA‑AAACCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCaaa                                                             <  1:220072/137‑1 (MQ=255)

                                                                                         ttttCTCCTGCGCTACTTTCTCGGCATAACAACCAGCAGCGTCATAATAACCGCCCCGCCCCGCCATTTTTTT‑AAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCa                                                   <  7:1017148/139‑1 (MQ=255)

                                                                                            tccccTCCGCTACTTTCTGGGAAAAACAAACATCAGCGGAATAAAAACCGCCCAGCCCCGGCATTTTATT‑AAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCAt                                                  <  6:150237/135‑1 (MQ=255)

                                                                                              tcCTGCGATACTTTCTCGGCATAACAAACATCAGCGGAATAATAAACGTCCAGCCCCGGAATTTTATT‑AAAACCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTgcg                                              <  2:305447/140‑1 (MQ=255)

                                                                                              cccggcgaTACTTTCTCGGCAAAAAAACCCTCTGCGGAATAAAAACCGCCCCGCCCCGG‑ATTTTATTAAAAACCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTgcg                                              <  3:521059/136‑1 (MQ=255)

                                                                                                  gcgcTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCg                                          >  7:155557/1‑140 (MQ=255)

                                                                                                  gcgcTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGAGCCCg                                          >  7:267516/1‑140 (MQ=255)

                                                                                                      aaCTTTCTCGGCATAACAAACATCAGCTGCATAATAACCGTCCAGCCCCGGAATTTAATA‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGAt                                      <  3:663204/139‑1 (MQ=255)

                                                                                                        cTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACACAGGCAAAATATTCCGCATTGCGCCCGCGATTg                                    >  3:401502/1‑140 (MQ=255)

                                                                                                          ttCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTg                                    >  5:599566/1‑138 (MQ=255)

                                                                                                           tctcGGAATAAAAAACATCCGCGGCAAAATAAACGTCCCGCCCCGGCATTTTATT‑AAACCAAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTgg                                   <  5:507791/138‑1 (MQ=255)

                                                                                                           gcccGGCAAAACAACCATCAGCGGCAAAATAAACGTCCCGCCCCGGATTTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTa                                 <  2:10172/137‑1 (MQ=255)

                                                                                                              cGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGAATTTTATT‑AACTCCAACGCCTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGc                              >  1:445974/1‑140 (MQ=255)

                                                                                                                 aaTAACAAACATCCGCGGAATAAAACCCGGCCCGCCCCGCAATTTAATT‑AAAGCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGAt                           <  1:980363/139‑1 (MQ=255)

                                                                                                                 aaTAACAAACATCAGCGGCATAATAAACGTCCACCCCCGGCATTTTATA‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGAt                           <  8:267516/139‑1 (MQ=255)

                                                                                                                        aaccaccgCGGAATAATAAAGCTCCAGCCCCTGATTTTTATT‑AAACCAAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                    <  7:120265/133‑1 (MQ=255)

                                                                                                                        aaccTCCGCGGCATAAAAAACGACCCCCCCCTGCAATTAATT‑AAAACCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCAGCATTGCGCCCGTGATTGGTATGCGATTTAAc                      <  5:539806/138‑1 (MQ=255)

                                                                                                                        aacaTCCGCGGAAAAATAACCGGCCAGCCCCGGTATTTTATT‑AAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                    <  4:545969/140‑1 (MQ=255)

                                                                                                                        aacaTCAGGGGTATATTAAAGCTGCTGCGCCGGCTTTTTTTT‑AAAACCTACGACTCTATAGACGGGGTGTTCACGTAAATGTTTTTTCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                    <  7:776348/140‑1 (MQ=255)

                                                                                                                        aacaTCAGCGGAATAATAACCGTCCCGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAAc                      <  5:97130/138‑1 (MQ=255)

                                                                                                                        aacaTCAGCGCCATAATACCCGTCCAGCCCCGGCATTTAATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                    <  2:666036/140‑1 (MQ=255)

                                                                                                                         aaaTCAGCGGCATAATAACCGCCCCCCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCgg                   <  7:723608/138‑1 (MQ=255)

                                                                                                                           aTCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATCTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGAGATTCGTATACGATTTAACCGGcc                 >  8:128177/1‑139 (MQ=255)

                                                                                                                                cGGAATAATAAACGCCCCGCCCCGGCATTTTATA‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTg            <  2:8745/140‑1 (MQ=255)

                                                                                                                                  gCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTATTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTCATTGGTATGCGATTTAACCGGCACtttt            >  5:483581/1‑137 (MQ=255)

                                                                                                                                  gCATAATAAACGCCCCGCCCCGGAATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGAt          <  7:920226/140‑1 (MQ=255)

                                                                                                                                  gCATAATAAACCTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTg            <  6:152954/138‑1 (MQ=255)

                                                                                                                                  ccATAATAAACGACCAGCCCCGTCATTTTATT‑AAACCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGAt          <  2:481740/139‑1 (MQ=255)

                                                                                                                                  caataataAACGACCATCCCCGGCATTTTATA‑AAATACAACGACTCTACCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGAt          <  4:278778/138‑1 (MQ=255)

                                                                                                                                   ctaaataatCGCCCCCCCCCGGCTTTTTATA‑AAACCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc         <  2:187303/137‑1 (MQ=255)

                                                                                                                                   cATAATAAACTTCCAGCCCCGGCATTTTATA‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGa           <  6:774661/138‑1 (MQ=255)

                                                                                                                                   aataataAACGTCCAGCCCGGGCATTTTATT‑AAATCACACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGa           <  5:799621/137‑1 (MQ=255)

                                                                                                                                   aataaaaACCGTCCCGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc         <  3:644749/139‑1 (MQ=255)

                                                                                                                                   aataaaaACCGTCCAGCCCCGGAATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc         <  4:441926/139‑1 (MQ=255)

                                                                                                                                   aataaaaACCGCCCACCCCCGCCATTTTATT‑AAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc         <  8:38548/139‑1 (MQ=255)

                                                                                                                                   aaaaatacACGCCCAGCCCCGGCATTTTATT‑AAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc         <  3:841298/137‑1 (MQ=255)

                                                                                                                                     taataaACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAg       >  7:403301/1‑140 (MQ=255)

                                                                                                                                      aataaACGTCCAGCCCCGGCATTTTATT‑AAATCCCACGACTCTATCGACGGGGGGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGt      <  4:828319/140‑1 (MQ=255)

                                                                                                                                          aaCGTCCCTCCCCGGCTTTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaa  <  1:691152/140‑1 (MQ=255)

                                                                                                                                           aCGTCCAGCCCCGGCTTTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCa   <  6:374664/138‑1 (MQ=255)

                                                                                                                                           aCGTCCAGCCCCGGCATTTTATT‑AAATCAAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCa   <  6:718741/138‑1 (MQ=255)

                                                                                                                                           aCGCCCACCCCCGGCATTTTATT‑AAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCa   <  5:846002/138‑1 (MQ=255)

CAGCAGCTGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTTATT‑AAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAA  >  NZ_CP022686/2546255‑2546532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A