Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F35 I0 R1 195 10216.4 8585726 98.3% 8439768 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,224 T→G 11.1% K188N (AAA→AAC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2240TG11.1% 276.1 / 8.0 163K188N (AAA→AAC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (37/103);  new base G (16/6);  total (54/109)
Fisher's exact test for biased strand distribution p-value = 4.21e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

CTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCC  >  NZ_CP022686/1674087‑1674359
                                                                                                                                         |                                                                                                                                         
cTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGTGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGttt                                                                                                                                         >  8:757402/1‑140 (MQ=255)
cTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGttt                                                                                                                                         >  1:1002068/1‑140 (MQ=255)
  ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGCCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGttt                                                                                                                                         >  6:292960/1‑138 (MQ=255)
   gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGtttttt                                                                                                                                      >  2:1089202/1‑139 (MQ=255)
   gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTGTAGTGTAGAGTCGGtttttt                                                                                                                                      >  7:93325/1‑139 (MQ=255)
        tcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑tt                                                                                                                               >  3:619670/2‑140 (MQ=255)
        gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGCCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTG‑tgatt                                                                                                                                >  7:715658/1‑136 (MQ=255)
        gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGTTATATCACTCATAACACTATCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTT‑TTC‑tt                                                                                                                               >  8:978544/1‑140 (MQ=255)
          aaaaaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCGACCAGGGCCTGACGGCGACGCTGAAACTCTTGCAGGGATATATCACTCATAACCCTCTCCGTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑tagt                                                                                                                             >  2:456615/1‑137 (MQ=255)
                   cgcgGCGCTGCCGGGTTGCATATGCTCTAACAGTGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATGACTCATAACACTCTCCTTACATTTTTTGTTTTTATTGTAGATTAGGTTTTTG‑TTC‑TTTTTgcggtgtc                                                                                                                    >  1:914074/1‑135 (MQ=255)
                     cggcgCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTGTTG‑TAC‑TTCTGGCGcggtc                                                                                                                    <  5:666973/138‑1 (MQ=255)
                      ggcgCTGCCGGGGTGCAGTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGTGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGcggtcggt                                                                                                                 <  8:531089/140‑1 (MQ=255)
                      ggagcTGCCGGTTTGCATTTGCTCTACCATGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCTCTAATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGcggtct                                                                                                                   <  6:303392/135‑2 (MQ=255)
                        cgcTGCCGGGTTGCATTTTCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTTCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTg                                                                                                               <  1:237714/140‑1 (MQ=255)
                        cgcTGCCGGGGTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTg                                                                                                               <  1:862123/140‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGAGGCTGAAACTCTTGCCGGTATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCgg                                                                                                            <  7:192837/140‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGc                                                                                                              <  6:766209/138‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCgg                                                                                                            <  1:492125/140‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCGCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCgg                                                                                                            <  3:82072/140‑1 (MQ=255)
                           tGCCGGGGTGCATTTGCTCTACCAGGGCCTGCCGGAGACGCTGAAACTCTTGCCGGTATATATCTCTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCTGTCGGTTGCgg                                                                                                            <  2:778044/140‑1 (MQ=255)
                             ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGAt                                                                                                          <  4:619670/140‑1 (MQ=255)
                             ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGAt                                                                                                          <  2:400920/140‑1 (MQ=255)
                             ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGGCGGTTGCGGAt                                                                                                          <  8:203529/140‑1 (MQ=255)
                             cagggTTGCATTTGCTCTAGCAGGGCCTGACGGCGACGCTGAAAGTCTTGCCGGGATATATCACTCATAACAGTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGAt                                                                                                          <  2:713381/138‑1 (MQ=255)
                                    gCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGGGGGTGAGTa                                                                                                     >  5:526603/1‑138 (MQ=255)
                                     aaTTTGCTCTACCAGGGGCTTTCGGCGAAGCTGAAACTGTTGCCGGGATATATCACTCATAACACTCTCCTTAAATTTTTTTTTTTTAGTGTAGAGTCGTTTTTTG‑TAC‑TTCTTGAGCGGTCGGTTGCGGATGAGTAAAg                                                                                                  <  3:42182/139‑1 (MQ=255)
                                         ttctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTTGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                              <  4:648787/138‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGTCGAAGCTGAAACTCTTGACTGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGGGTAGAGTCTGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                              <  7:959205/140‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGGCGCCGCTGAAACTCTTGCAGGGATATATAACTCATATCACTCTCCAGACTTTTTTTGTTTTTAGTGTATAGTCGGGTTTTT‑TAA‑TGGATGAGCTGGCGGTTGCTGATGATTaaaaat                                                                                                >  6:187115/1‑135 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                              <  8:247781/140‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                              <  2:919605/140‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                              <  1:519440/140‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                              <  2:817871/140‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                              <  4:893811/140‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                              <  4:706279/140‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                              <  2:1002068/140‑1 (MQ=255)
                                           ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                              <  6:1020042/138‑1 (MQ=255)
                                               aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TTA‑TTATTGCGGGGGAGGTTGAGGATGAGTAAATTGGTCGTGGc                                                                                        >  8:197778/1‑140 (MQ=255)
                                                ccAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCGTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTG‑TTC‑TTGTGGCGCGGGGGGTTGGGGATGATTAAAGGTGTCGGGGCa                                                                                       >  2:881225/1‑140 (MQ=255)
                                                   gggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATaa                                                                                    <  8:423763/140‑1 (MQ=255)
                                                        tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTT‑TAC‑TTGTGGCGCGGTCGGTTGCGGATTTGTAAAAGTGGGGGGGCATAAAAgcg                                                                               >  2:382499/1‑140 (MQ=255)
                                                        tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGGGGATGAGTAAATGGGTCGTGGCATAACAgcg                                                                               >  1:658360/1‑140 (MQ=255)
                                                           cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                            <  1:765489/140‑1 (MQ=255)
                                                           cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                            <  2:15896/140‑1 (MQ=255)
                                                           cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTATCCTTACATTTTTTGTTTTTAGTGTAGAGGCGGTGTTTG‑TAC‑TTCTGGCGCGGGCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcgc                                                                              <  6:119340/138‑1 (MQ=255)
                                                            ggCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGc                                                                           <  4:1063030/140‑1 (MQ=255)
                                                              cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGc                                                                           <  6:616248/138‑1 (MQ=255)
                                                                aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                       <  1:1000082/140‑1 (MQ=255)
                                                                   cTGAACCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACgg                                                                    <  2:642525/140‑1 (MQ=255)
                                                                   cTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAacac                                                                      <  5:632478/138‑1 (MQ=255)
                                                                   cTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACgg                                                                    <  3:840839/140‑1 (MQ=255)
                                                                      aaaCTCTTGCCGGGATATATCACTCATAACACGCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACg                                                                 <  2:988521/140‑1 (MQ=255)
                                                                         ctctTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTAc                                                              <  1:974535/140‑1 (MQ=255)
                                                                          tctTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACt                                                             <  7:1004348/140‑1 (MQ=255)
                                                                               ccGGGATATCTCACTCCTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTG‑TCC‑TTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGGGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                        <  4:39705/140‑1 (MQ=255)
                                                                               ccGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                        <  4:441678/140‑1 (MQ=255)
                                                                                 gtgATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGGCGGGGGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                      <  7:628312/138‑1 (MQ=255)
                                                                                 gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCTGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                        <  6:660892/138‑1 (MQ=255)
                                                                                 gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                        <  5:146261/138‑1 (MQ=255)
                                                                                 gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                        <  5:517056/138‑1 (MQ=255)
                                                                                 gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTGTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCTGGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                      <  4:438826/140‑1 (MQ=255)
                                                                                        atCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAA‑TTCGGGGGCGGGGGGGTGCGGTTGTGTAAAAGTGTCGTGGGATAACATCGCGGCAACCCGGACGTACTCGCTGAGCTCttga                                               >  3:557493/1‑138 (MQ=255)
                                                                                            cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                           <  4:418924/140‑1 (MQ=255)
                                                                                            cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTATTGCGCGGTCGGTTGCGGGTGAGTAAAAGTGTGGTGGGATAACAGCGCGGCACCACGGACGTAATCGCAGATATAGTCAAGCg                                           >  2:1100937/1‑140 (MQ=255)
                                                                                             tCATAACACTCTCCTTACCTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCTGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGc                                            <  5:864928/138‑1 (MQ=255)
                                                                                             tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTGGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                          <  3:398746/140‑1 (MQ=255)
                                                                                             acATAACACTCTCCTACCATTTATTGTTTTTAGTGTAGACTCGGTTTTTG‑TAC‑TTCTGGCGCTGTCGGTTGCGGATGCGTAAAGGTGTCGTGTGATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGc                                            <  6:852581/137‑1 (MQ=255)
                                                                                               aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTAG‑TGGTGG‑GCGGTCTGTTGGGGATGAGTTAATGTGTAGTGGCATAACAGCGCGGCAACCCGGGCGTACTCGATTATATCTTAAAGCGACa                                        >  8:156347/1‑140 (MQ=255)
                                                                                               aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTG‑TTA‑TTCTGGGGCGGTGGGGTGGGGGTGCGTTAACGGGTCGTGGCATAACAGGGCGGCCACACGGACGTTCTCGCTGATGTATTCAAGCgg                                          >  5:839657/1‑137 (MQ=255)
                                                                                               aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGATTTG‑TAC‑TTCTGGCGCGGGCGGTTGCGGGTGAGTAAAGGGGTCGTGGGATAACAGCGCCGCCCCACCGACGTACTACATGTTCTCTTCAAGCGAcc                                        >  3:1056671/1‑139 (MQ=255)
                                                                                                 aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTG‑TAC‑GTATTGGGCGGGGGGGTGCGGATGGGGAAAGGTGGCGTGGCCTAACAGCGCGGCAACCCGGGCGTACTCGGAGGACTCTTCAAGCGACa                                        >  6:857357/1‑138 (MQ=255)
                                                                                                 aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGACGGTTTTTT‑TAA‑TTCTTGCGAGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCAAAAAAGCCCTGAAAAAAGGAAGAACTCAATTATCAATACAAGCGACaat                                      >  8:307762/1‑138 (MQ=255)
                                                                                                   cacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTT‑TTC‑TTATGGGGCGGTGGGATGCGGATTAGTTAACGTGTAGGTGCATAAAAACGCGGCAACACCGACGGTATCGTTGATACCTTAAAGCGACAatta                                    >  8:704558/1‑139 (MQ=255)
                                                                                                    acTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCa                                   <  1:326404/140‑1 (MQ=255)
                                                                                                    acTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCa                                   <  3:31950/140‑1 (MQ=255)
                                                                                                      tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                 <  7:417649/140‑1 (MQ=255)
                                                                                                      tctcCTTACATATTTTGTTTTTAGTGTAGAGTCGGATTTTG‑TAA‑ATCTGTGGCCGTCGGTTGCTGATGAGTAAACTTGTCGTTGCATAACACCCCGGCCACACCGCCCTACTGCATGCTCTCGTAAAGCGACCGTTCAAg                                 >  4:617734/1‑140 (MQ=255)
                                                                                                      gctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTGTG‑TAC‑TTCTGGCGCGGGCGGGTGCGGATGAGTACAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGTTCTCTTCAAGCGACATTTCAAg                                 <  3:227025/139‑1 (MQ=255)
                                                                                                       ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTT‑TAC‑TTCTGGCGCGGGCGGTTGCGGATGAGTTAAGTTGTCGTGGCATAGCAACGCGGCCACACGGACGTACTCGATGATCTCTTCAAGCGACATTTAAAGc                                >  8:82597/1‑140 (MQ=255)
                                                                                                       ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TTC‑TTATGGCGCGGTCGGGTGCGGATTCGTTAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGGACGAGATGATATATTCAAGCGACATTTGAAGc                                >  8:574546/1‑140 (MQ=255)
                                                                                                       ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                <  1:1095044/140‑1 (MQ=255)
                                                                                                       ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                <  4:1074898/140‑1 (MQ=255)
                                                                                                       ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑GTAAGGCGCGGTAGGTTGCGGATTATTAAAGGTGTAGTTGCAAAAAAGCGCGGCCACCCGGCCGGACACCAGGATCTCTTCAAGCGACATATCAAGc                                >  4:815898/1‑140 (MQ=255)
                                                                                                       ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTT‑TAC‑TTCTGGCGCGGTCGGTTGGGGATGTGTAAAGGTGTCGTGGCATAACAGCGCGTCAACACGGACGTACTCGATGATATCTTCAAGCGACATTTAAAga                                >  2:293642/1‑139 (MQ=255)
                                                                                                       ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTG‑TAC‑TTCTGGCGCGGTCGGTTTGGGATGAGTAAAGGTGTAGTTGCATAACACCCCCGCCACAAGCACGGACTCGATGATCACTTGAACAGACATTTCAAGc                                >  2:779739/1‑140 (MQ=255)
                                                                                                            tACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTG‑TAC‑TTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTc                           <  7:5421/140‑1 (MQ=255)
                                                                                                             aCATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTT‑TTC‑TTCGGGCGCGGTCGGTGGCGGATGAGTAAAAGTGTCGTGGGCTAAACGCGCGGCAACACGGACGGACTCGATGATATCTTGAACCGACATTTCAATctagtcc                          >  2:231653/1‑135 (MQ=255)
                                                                                                               aTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTT‑TTCTTTGTGGC‑CGGGAGGGAGCGGATGAGGAAATGTGTCTTGGCATAACAGCTCGGCAACACGGACGGACAGGATGAACTCTTCAAGCCACATTACAAACTCttag                          >  6:55133/1‑136 (MQ=255)
                                                                                                                   tttGTTTTTAGTGTAGAGTCGGTTTTTT‑TAA‑TTAAGGCGCGGTCGGGTGCGGATGAGTTAAGGTGTAGTGGCATAACAACGCGGGAACACGGCCGTACTCCATGATATCTTAAATCGACATTTAAAGCTCTTACTGAtct                    >  2:591719/1‑140 (MQ=255)
                                                                                                                    ttGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACg                                                                 <  3:565854/94‑1 (MQ=255)
                                                                                                                    ttGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACg                                                                 >  4:565854/1‑94 (MQ=255)
                                                                                                                         tttAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGTCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgt                <  6:680566/138‑1 (MQ=255)
                                                                                                                         tttAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtct              <  8:474762/140‑1 (MQ=255)
                                                                                                                             gtgtAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAgcttcgtcttc            <  6:415953/138‑1 (MQ=255)
                                                                                                                             gtgcAGAGTCGGTTTTTG‑TAA‑TTCTGGGGCGTTCGGTTGGGGACTAGTTTAGGTGTCGTGGCATAAGAGCGCTGCAACACGGACGTTCTCGATGATCTCTTCAAGCGACATTTGAAGCTCTGCCTGAtcttcgtattc            <  6:357275/138‑1 (MQ=255)
                                                                                                                               gtAGAGTCTGTTTTTG‑TAC‑TTCAGGCGCGGTCGGTTGCGTATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTtcgtcg        <  3:743864/140‑1 (MQ=255)
                                                                                                                               gtAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCTGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGTCAACACGGACGTACTCGAGGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTtcgtcg        <  1:236720/140‑1 (MQ=255)
                                                                                                                                  tagTCGGGTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTcgtcgt       <  5:740735/137‑1 (MQ=255)
                                                                                                                                  gagTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGACCTTCGTCTTCGTCGTaa     <  3:866959/140‑1 (MQ=255)
                                                                                                                                     gcGGGTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAAccc  <  2:908700/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
CTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTG‑TAC‑TTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCC  >  NZ_CP022686/1674087‑1674359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A