Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F36 I0 R1 179 8076.5 8499890 98.3% 8355391 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,224 T→G 22.4% K188N (AAA→AAC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2240TG22.4% 207.9 / 25.9 148K188N (AAA→AAC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (28/83);  new base G (30/6);  total (58/90)
Fisher's exact test for biased strand distribution p-value = 1.08e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

GGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAA  >  NZ_CP022686/1674089‑1674361
                                                                                                                                       |                                                                                                                                           
ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGttttt                                                                                                                                         >  6:137518/1‑140 (MQ=255)
ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGttt                                                                                                                                           >  7:850204/1‑138 (MQ=255)
cgTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTAGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCAGGGATATATAACTCATAACACTCTCCTTACATATATTGATTTTAGTTTAGAGTCGGttttt                                                                                                                                         >  2:558905/2‑140 (MQ=255)
 gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTg                                                                                                                                        <  4:153688/140‑1 (MQ=255)
 gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTATGTTTTTAGTGTAGAGTCGGgttttt                                                                                                                                        >  2:44197/1‑139 (MQ=255)
  tGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGttttt                                                                                                                                         >  7:114732/1‑138 (MQ=255)
  tGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGttttt                                                                                                                                         >  7:197050/1‑138 (MQ=255)
    cagcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGtta                                                                                                                                     >  1:1035403/1‑138 (MQ=255)
      gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTTCTCTACCAGGGCATGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACtt                                                                                                                                   >  6:532316/1‑140 (MQ=255)
      gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGgtttttttatt                                                                                                                                   >  5:277826/1‑134 (MQ=255)
            aTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGgcg                                                                                                                             <  3:975532/140‑1 (MQ=255)
            aTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGgcg                                                                                                                             <  2:750520/140‑1 (MQ=255)
               cgcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGTATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGcgg                                                                                                                          <  6:116274/139‑1 (MQ=255)
                gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGcggt                                                                                                                         <  1:44197/140‑1 (MQ=255)
                ccgcgGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGTAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTAGGTTTTTGTACTTCTGGcgcg                                                                                                                           <  8:269183/137‑1 (MQ=255)
                 ggaggcgCTGCCGGGTTGCATTTGATCTACCAGTGCCTGACGGCGACGCTGAATCTCTTGCCGGGATATATCACTCATAACACTCTCCTTAGATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGcggtc                                                                                                                        <  6:628552/137‑1 (MQ=255)
                     gcgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGtt                                                                                                                    <  4:388189/140‑1 (MQ=255)
                         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                <  6:336978/140‑1 (MQ=255)
                         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                <  4:951997/140‑1 (MQ=255)
                         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                <  2:395961/140‑1 (MQ=255)
                         tGCCGGGTTGCATTTGCTCAACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                <  6:2500/140‑1 (MQ=255)
                         tGCCGGGGTGGATTTGCTCTACAAGGGCCTGTCTGCGACGCTGAAACTCTTGCCGGGATATAGCACTCATAACACTATCCTTAGATTATTTTTTTTTCGTGTAGCGTAGGGTTTTGTACTTCTGGCGCGGTCCGTTGctg                                                                                                                <  4:189086/140‑3 (MQ=255)
                           ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATAGCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGGGTAGAGTCGGGTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑At                                                                                                             <  4:688733/140‑1 (MQ=255)
                            cGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATg                                                                                                            <  3:475879/140‑1 (MQ=255)
                                   cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTAATTATGGCGCGGTCGGGTTCGG‑ATGA‑GTtaat                                                                                                    >  1:72299/1‑139 (MQ=255)
                                       tGCTCTAGCAGGGCCTGACGGCGACGCTGAAACTCTTGACGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGgtgt                                                                                                <  2:869564/140‑1 (MQ=255)
                                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGgtgt                                                                                                <  5:533129/140‑1 (MQ=255)
                                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGgtgt                                                                                                <  2:60541/140‑1 (MQ=255)
                                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGgtgt                                                                                                <  3:655312/140‑1 (MQ=255)
                                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCg                                                                                              <  2:914612/140‑1 (MQ=255)
                                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCg                                                                                              <  3:1020720/140‑1 (MQ=255)
                                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCg                                                                                              <  1:98226/140‑1 (MQ=255)
                                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCg                                                                                              <  4:188155/140‑1 (MQ=255)
                                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTAATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCg                                                                                              <  1:967480/140‑1 (MQ=255)
                                         ctctAAGAGGGCCTGACTGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCATTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCg                                                                                              <  4:923971/140‑1 (MQ=255)
                                            aaCCAGGTCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑ATAAAGGTGTCGTgg                                                                                           <  5:703608/139‑1 (MQ=255)
                                              ccAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTCTTCTGGCGCGGGCGGTTGGGG‑ATGA‑TGAAATgtgtgctggga                                                                                         >  3:532905/1‑133 (MQ=255)
                                                  ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTAAATCTTGCGCGGTCGGTTTCGG‑ATGA‑GTAAAGGTGTCGTGGGATaaa                                                                                     >  4:310818/1‑139 (MQ=255)
                                                   gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGGTTTTTTGTTATGGCGCGGTGGGTGTGGG‑ATTA‑GGAAATGTGGCGTGGGAttaac                                                                                    >  1:74530/1‑136 (MQ=255)
                                                      tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAAGTATTGGGCGGGCGGTTGGGG‑ATGA‑GTAAAGGGGTCGTGGGATAACAgcg                                                                                 >  3:889252/1‑140 (MQ=255)
                                                      tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTATTGTGGCGCGGTCGGTTGCGG‑ATGG‑GTATATGTGGAGTGGCATCACAgcg                                                                                 >  3:894849/1‑140 (MQ=255)
                                                       gACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAgcgc                                                                                <  3:728090/140‑1 (MQ=255)
                                                       gACGGCGACGCTGAAACTCTAGCCGGGATATATCACTCATAACACTCTCATTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGGGGTCGGTTGCGG‑ATGA‑GTAAAGGGGGCGTGGCATAACAgc                                                                                  <  8:1008433/138‑1 (MQ=255)
                                                        ccGGCGACGCGGAAACTCTTGCCGTGAGATATCTCTCATCCCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGGTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGcgcg                                                                               <  4:554323/139‑1 (MQ=255)
                                                        aCGGCGACTCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTGGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGcgcg                                                                               <  2:602211/140‑1 (MQ=255)
                                                        aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTGTGGCGCGGTCGGGTGCGG‑ATGA‑GTAAATGTGTCGTGGCATAACAGcgcg                                                                               >  3:46087/1‑140 (MQ=255)
                                                        aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTAGTCTTGGGCGGGGGGTGGGGG‑TTGA‑GGATAGGGGGAGTGGGAGATCAGcgcg                                                                               >  5:465566/1‑140 (MQ=255)
                                                         cgtcGACGCTGAAACTCTTGACGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCgg                                                                              <  3:841702/137‑1 (MQ=255)
                                                         cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCgg                                                                              <  6:668278/140‑1 (MQ=255)
                                                              aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                         <  6:1043157/140‑1 (MQ=255)
                                                              aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                         <  4:953038/140‑1 (MQ=255)
                                                                    aaaCTCTTGCCGGGATATATCACTCATTACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTCAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACg                                                                   <  1:696033/140‑1 (MQ=255)
                                                                    aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACg                                                                   <  1:851170/140‑1 (MQ=255)
                                                                    aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTAAATCTTGCGCGGTCGGATGCGG‑TTTA‑GTAAATGTGTAGTTGCAAAACAACCCCGCCacaccgtcc                                                                   >  5:634299/1‑135 (MQ=255)
                                                                       ctctTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTAc                                                                <  4:941749/140‑1 (MQ=255)
                                                                          ttGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAAGTCTGGCGCGGTCGGTTGGGG‑ATTA‑GTTAAGGTGTCGGGGGATAACCTCGCCGCCACACCGCCGGACTCg                                                             >  6:24785/1‑140 (MQ=255)
                                                                          ttGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTATTAGTGTAGAGTCGGTTTTTGTAATTATGGCGCGGTGGGTTGCGG‑TTTA‑GTTAATGTGTCGCGGAGAAACAGCCCGGCAACACTGACGAACACg                                                             >  1:155077/1‑140 (MQ=255)
                                                                             ccGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                          <  2:126930/140‑1 (MQ=255)
                                                                                      atCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTTTAGAGTCGGTTTTTGTACTTCTGGCTCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCtt                                                   <  7:344415/138‑1 (MQ=255)
                                                                                          cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCTGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                             <  3:862635/140‑1 (MQ=255)
                                                                                          cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGGGCGGTCGGGTGCGG‑ATGA‑GTAAAGGTGTGGTGGCATAACAGCGCGGCAACACGGCCGTACTCGATGATCTCTTGAAGCg                                             >  4:526665/1‑140 (MQ=255)
                                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTCGGGGGCGGTCGGGTGGGG‑ATGA‑TTTAAGGTGTCGTGGCATAACAGCGCGGCAACACGGAAGGACACGATGAAGTCTTCacgggc                                            >  4:748688/1‑135 (MQ=255)
                                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTACATCTGGGGCGGTCGGGTGCGG‑ATGA‑GTAAATGTGGCGTGGCATAAAAGCGCGGCAAAACGGACGTACAAGATGATGACTTAAAGCGa                                            >  2:481536/1‑140 (MQ=255)
                                                                                            cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGGGGGGTGGGG‑ATGA‑GGAAAGGTGGAGTGGGATAAAAGCGCGGCAACACCGACGTACTCGATGATCTCTTCAAGCGAc                                           >  1:334326/1‑140 (MQ=255)
                                                                                             aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGGTTTTTACGTATGGCGCGGGAGGTTGCGG‑ATGA‑GTTAAGGTGTGGGGGGATAAACGCGCCGCAACACCGCCCTACTCGATGATGTCTTAAAGCGa                                            >  8:419427/1‑138 (MQ=255)
                                                                                              tAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGGCGGTTGAGG‑ATTA‑GGAAATGTGTCGTGGCATAACAGCGCGGACACACGGACGTACAGGATGATAGCTTAAAGAGACAt                                         >  5:796952/1‑140 (MQ=255)
                                                                                               aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAAATATTGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCTGGGCAAAAAATCGAGGCAACACCGACGTAATCCATGCTCTCTTCAAGCGAcc                                          >  8:102589/1‑137 (MQ=255)
                                                                                               aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTACTTATGGCGCGGTCGGTTGGGG‑GTGA‑GTTAAGGGGTGGTGGCAGAACAGCGCGGCCACACGGACGGACTCGATGATCTCTTCAAGCGCCAtt                                        >  2:405655/1‑140 (MQ=255)
                                                                                               aaCACTCTCCTTACATTTAATGTTTTAAGTGTAGAGTCGGTTTTTTTAATTATTGCGCGGTCGTGTGCGG‑AAGA‑GTAAACTGGTAGTGGCATAAAATCGAGGCAACACGGACGTACGAGATGATGTGTTAAAGCGAct                                          >  8:430944/1‑137 (MQ=255)
                                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTATGGCGCGGGGGGTTGGGG‑ATGA‑GTAAAGGTGTAGTGGCCTAACAGCGCGGCCACACGGACGTACTCGATGAGATCCTCAAGCGAAATTTCaaac                                  >  5:87774/1‑138 (MQ=255)
                                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTGCTTCGGGCGCGGTCGGGGGCGGCATG‑‑GTAAAGGTGTGGGGGCAGAACAGCCCCGCCACAAGGCCGTACGAGGTGATGACGTCTCGCGACAtttgcagc                                  >  4:226393/1‑135 (MQ=255)
                                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCaa                                    <  8:773535/138‑1 (MQ=255)
                                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCaa                                    <  7:448851/138‑1 (MQ=255)
                                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                  <  4:599050/140‑1 (MQ=255)
                                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                  <  1:570294/140‑1 (MQ=255)
                                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                  <  3:325857/140‑1 (MQ=255)
                                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTAGGTTGCGG‑ATGA‑GTAAAGGTGTAGTGGCATAACAGCGCGGCAACACGGACGTACTCGAAGATCTCTTCAAGCGACATTTCAAGc                                  >  4:648870/1‑140 (MQ=255)
                                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTGTTTGTACATAAGGCGCGGTCGGTTGCGG‑AAGA‑CTAAATGTGTGGTGGCCAAACAGCCCCGCCACACGGAACTACTCGGTGATCTCTTCAATCGACATTTCAAGc                                  >  4:734638/1‑140 (MQ=255)
                                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTTAATCTGGCGCGGTAGGGTGTGG‑ATTATGTAAA‑GTGTGTGGGGATAAACCCGCGGCAACACCGTCGTCCTCGAAGAAAAGATCAAGAGACAATTCaacc                                  >  5:17993/1‑138 (MQ=255)
                                                                                                        cTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTCTTGCGCGGTCGGTTTCGG‑ATTA‑GTAAAGGTGTCGGGGCCTAACAGCGCGGCCACACGGACGGACAACATGATCTCTTCAAGCGCCATTTCAAGctct                               >  6:379126/1‑140 (MQ=255)
                                                                                                           aCATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                            <  2:728138/140‑1 (MQ=255)
                                                                                                            cATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCt                           <  1:1033527/140‑1 (MQ=255)
                                                                                                            cATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTACTTCTGGCGCGGTCGGGTGCGG‑AAGA‑GTAAAGGTGTAGGGGCATAACAGCGCGGCAACACCGACGGACGCGAAGATCTCTTCAAGCGACATTTCAAGCTCttgca                           >  4:21031/1‑137 (MQ=255)
                                                                                                                  ttGTTTTTAGTGTAGAGTCGGGTTTTGTTCGTCTGGCGCGGTCGGTTGGGG‑ATGA‑GTTAAGGGGTCGTGGGGTAACAGCGCGGCAGCACGGCCGGACGCGATGCTCTCGTCAAGCGACATTTCGAGCGGTTCCGGAtctt                     >  6:453790/1‑140 (MQ=255)
                                                                                                                    gTTTTTAGTGTAGAGTCGGTTTTTTTAATTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGGCGTACTCGATGATATCTTAAAGCGCCATTTAAATCTCTTACTGAtctt                     >  8:703549/1‑138 (MQ=255)
                                                                                                                       tttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgt                  <  7:835753/138‑1 (MQ=255)
                                                                                                                       tttAGTGTAGAGTCGGGTTTTGTACTGCTGTCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGGCGGGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgt                  <  7:539505/138‑1 (MQ=255)
                                                                                                                        ttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt               <  5:551146/140‑1 (MQ=255)
                                                                                                                        ttAGTGTAGAGTCGGTGTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt               <  2:66269/140‑1 (MQ=255)
                                                                                                                          aGTGTAGAGTCGGGTTTTGTTCTTGTGGCGCGGGCGGTTGGGG‑ATGT‑GGAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATGTATTAAAGCGCCATTTAAAGCTCTTCCAGAacttcgtcttcg             >  4:216488/1‑140 (MQ=255)
                                                                                                                           gtgtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc              <  8:87517/138‑1 (MQ=255)
                                                                                                                           gtgtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt            <  1:903740/140‑1 (MQ=255)
                                                                                                                           gtgtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt            <  6:687542/140‑1 (MQ=255)
                                                                                                                           gtgtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt            <  6:1002718/140‑1 (MQ=255)
                                                                                                                             gtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTtggtcg          <  4:144124/140‑1 (MQ=255)
                                                                                                                             gtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTtcgtcg          <  6:829663/140‑1 (MQ=255)
                                                                                                                                     ggTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCTCGGCAACACGGACGTACTCGATGAGCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa  <  4:801480/140‑1 (MQ=255)
                                                                                                                                     ggTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa  <  1:22507/140‑1 (MQ=255)
                                                                                                                                     ggTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa  <  3:102896/140‑1 (MQ=255)
                                                                                                                                     ggTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGGGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCGCTTCCTGATCTTCGTCTTCGTCGTAACCCaa  <  4:48617/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                           
GGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGG‑ATGA‑GTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAA  >  NZ_CP022686/1674089‑1674361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A