Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F13 I0 R1 203 11425.2 8793538 98.4% 8652841 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,572,705 A→C 11.7% G191G (GGT→GGG)  CIW80_RS12885 ← ROK family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,572,7050AC11.7% 402.2 / 10.4 216G191G (GGT→GGG) CIW80_RS12885ROK family protein
Reads supporting (aligned to +/- strand):  ref base A (117/67);  new base C (2/29);  total (119/97)
Fisher's exact test for biased strand distribution p-value = 8.86e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

GGTTGCCAGCGTGCGGGCAAGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAAC  >  NZ_CP022686/2572567‑2572843
                                                                                                                                           |                                                                                                                                          
ggTTGCCAGCGTGCGGGCAAGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAAc                                                                                                                                           >  1:676558/1‑140 (MQ=255)
ggTTGCCAGCGTGCGGGCAAGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAAc                                                                                                                                           >  6:488206/1‑140 (MQ=255)
 gTTGCCAGCGTGCGGGCAAGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAAcc                                                                                                                                          >  8:322449/1‑140 (MQ=255)
         cGTGCGGGCAAGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACACGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGGCATCTGTTGATAGCGTTCCC‑TAAAACCGCTGCCgg                                                                                                                                  >  2:718552/1‑140 (MQ=255)
         cGTGCGGGCAAGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCgg                                                                                                                                  <  5:1058815/140‑1 (MQ=255)
             cGGGCAAGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTGATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGagac                                                                                                                              >  2:317827/1‑140 (MQ=255)
               ggCAAGCTTTTGGAGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCAGAAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGaaacaa                                                                                                                            >  5:315065/1‑140 (MQ=255)
                   aGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGTaaacaaac                                                                                                                          <  4:1061651/138‑1 (MQ=255)
                          ggCGAAACCGTCCCACCTTATGATTGGCACAAGCATCACAACTTTTTGCCAGTGTGACAATCGCCGCATAATTGAGAATTTTACCATTCATCTGTTTAAAGCGTACAC‑TAAAACCGCTGCCGGAAACACACGACTCCACg                                                                                                                 <  2:1103904/140‑1 (MQ=255)
                          ggCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACg                                                                                                                 >  5:210556/1‑140 (MQ=255)
                            cGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACg                                                                                                                 >  3:551715/1‑138 (MQ=255)
                                aaCGTGCCACCTGCTGCATGGCACAAGCATCCCCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTAAC‑TAAAACCGCTGCCGGAAACAAACGACTCAACGCAGt                                                                                                             >  4:249737/1‑138 (MQ=255)
                                       cACCTGCTGCATGGCACAAGCATCACCACTTTGTGCCAGGGTGAAAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTTATACCGTTCAC‑TAAAACCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGc                                                                                                    <  2:454376/140‑1 (MQ=255)
                                                  tGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCaa                                                                                         >  7:339109/1‑140 (MQ=255)
                                                  gggCACAAGCATCACCCCGTTGTGCCTGGGTGAAAACCGCCGCAGAAGGGAGCAAGTTACCCGTCATCTGTTTATAGCGTTCAC‑TAAACCCGCTGCCTGAAAAACACGACTCCACGCAGTTGTGTTTGCCGCAATAGc                                                                                           <  4:747010/137‑1 (MQ=255)
                                                     cacaAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGCAGTGAGCAACTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAac                                                                                        >  4:853899/1‑138 (MQ=255)
                                                     cacaAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGCAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAac                                                                                        >  4:507307/1‑138 (MQ=255)
                                                       caAGCATCACCACGTTGTGCCAGGGTGCCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGAGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAactgac                                                                                    >  6:800906/1‑140 (MQ=255)
                                                       caAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAactgac                                                                                    >  2:258102/1‑140 (MQ=255)
                                                               accacGTTGTGCCAGGGTGACACTCGCCGCCGCAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGGTCAC‑TAACCCCGCTGCCGGAAACAAACGACTCCCCGCAGTTGTGTTTGCCGCAATATCAACTGCCTGATGGAc                                                                            >  6:718827/1‑140 (MQ=255)
                                                               accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACTACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTCACTGATGGAc                                                                            >  2:641186/1‑140 (MQ=255)
                                                               accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCCCGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                            >  5:828386/1‑140 (MQ=255)
                                                               accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATgg                                                                              >  4:335282/1‑138 (MQ=255)
                                                               accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                            >  8:304975/1‑140 (MQ=255)
                                                               accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                            >  1:560647/1‑140 (MQ=255)
                                                               accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                            >  5:974023/1‑140 (MQ=255)
                                                               accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGACACTGACTGATGGAc                                                                            >  6:1066206/1‑140 (MQ=255)
                                                                 cacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTTAGCACGTTACAAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCGGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCa                                                                          <  8:459113/140‑1 (MQ=255)
                                                                 cacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                            >  4:549892/1‑138 (MQ=255)
                                                                 cacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGGTGTGATTGCCGCAATAGCAACAGACTGATGGAc                                                                            >  3:27241/1‑138 (MQ=255)
                                                                 cacGATGTGCCAGGGTGACAATCGCCGAAGAAGTGAGAAAGATACCAGTAATCTGTTGATAGCGATCAC‑TAAAACAGCTGCCGGAAACAAACGACTCCAAGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                            >  4:520711/1‑138 (MQ=255)
                                                                 aaggTTGTGCCTGGGTGACAATCGCCCCAGAAGTGCGCAAGTTACAAGTCATATGTTTATAGCGTTCAA‑TAAAACCGCTGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                            <  4:102841/135‑1 (MQ=255)
                                                                      tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCt                                                                       >  3:1105830/1‑138 (MQ=255)
                                                                      tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                     <  7:304975/140‑1 (MQ=255)
                                                                      tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCCGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                     >  7:811018/1‑140 (MQ=255)
                                                                      tgtgCCAGGGTGACAATCGCCGCAGAAGAGAGCAAGTTACCAGTCATCTGTTGATAGCGATCAC‑TAAAACCGCTGCCGGAAACAAACCACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTCACTGATGGACCATCTTc                                                                     >  7:1008478/1‑140 (MQ=255)
                                                                      tgtgCCAGGGTGACAATCGCCGAAGACGTTACCAAGTAACCAGGCATCTGTTTATCGCGTACAC‑AAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                     <  2:205456/140‑1 (MQ=255)
                                                                      tgtgCCAGGGGGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTGGTGTTTGCCGCAATAGCAACTGAATGATGGCCCATCtac                                                                     >  7:998820/1‑138 (MQ=255)
                                                                      gttgCCCGGGTGACAATCGCCGCAGAAGTGAGCTAGTTACCAGTCATCTGTTGATAGCGATCAC‑TAAACCCCCTGCCGGAACCAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                     <  8:169038/138‑1 (MQ=255)
                                                                        tgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCt                                                                   >  5:851784/1‑140 (MQ=255)
                                                                         gCCTGGGTTACAATCGCCGCATAAGTTAGCAATTTACCAGGCTTCTGTTAATCGCTTTCAA‑AAAAACGGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTcc                                                                    <  3:1073452/138‑1 (MQ=255)
                                                                         gCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                  <  6:851784/140‑1 (MQ=255)
                                                                         gCCAGGGGGACACTCGCCGAAGACGTGAGCAAGTTACCAGGCATCTGTTGATTGCGTTCAC‑TAAACCGGCTCCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                  <  2:838148/140‑1 (MQ=255)
                                                                         cacAGGGTCAAAATCGCCGCACAAGGGACCCAGTAACCAGTACACTTTTTATTGCGTGCAA‑TAAACCCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                  <  7:919433/138‑1 (MQ=255)
                                                                           ctgggTGACTCTGGCCGCAGAAGGGAGCAAGTTACCAGTCATCTGTTGATCGGGTTCAC‑AAAAACCGCGGCCGGATACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                <  2:439855/138‑1 (MQ=255)
                                                                           ctgggTGACATCCGCCGCAGACGTGATCAAGTTACCAGTAATCTTTTTATAGCGTCCAA‑TAAAACCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                  <  3:566114/136‑1 (MQ=255)
                                                                           cAGGGTTACAATCGCCGAATAAGTGCCAATTTTACACGGATTTTGTTTATCGCGTCCAA‑TAAACCCTCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                  <  3:1016932/138‑1 (MQ=255)
                                                                           cAGGGTTAAATTCGCCGCAGACGTTAGCAAGTTACCAGTCATCTTTTGATACCGTTCAA‑TAAAACCGCTTCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                <  8:673715/140‑1 (MQ=255)
                                                                           cAGGGTGACAAGCGCCGCAGATGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑AAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                <  8:626919/140‑1 (MQ=255)
                                                                              ggTGACATGCGCCGCATTTGTGAGCATGTTACCAGCCATCTGTTTATAGCGTTCAC‑TAAACCCGCTGCCGGAAAAAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                             <  8:808835/140‑1 (MQ=255)
                                                                                ttaCAATCGCCGCACAAGTCAGCAAGTTACCTGTCTTCTGTTTATAGCGTTCAC‑TAAAACCGCGGCCGTAAAAAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                             <  4:168799/136‑1 (MQ=255)
                                                                                tcaCAAGCGCCGCAGACGTGCGCAAGTTACCAGTCATCTGTTGATAGCGTCCAC‑TAAACCCGCGGCCGCAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGta                                                           <  7:220212/138‑1 (MQ=255)
                                                                                tGACAAGCGCCGAATAAGTGAGCAATTTACCCCTCATCTTTTGATAGGGTTAAC‑TAAACCCGCTGCCGTAAAAAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                             <  3:943655/138‑1 (MQ=255)
                                                                                  aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCTCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                         >  6:453036/1‑140 (MQ=255)
                                                                                  aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                         >  1:44033/1‑140 (MQ=255)
                                                                                  aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                         >  7:390376/1‑140 (MQ=255)
                                                                                  aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                         >  6:955000/1‑140 (MQ=255)
                                                                                  aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                         >  6:246584/1‑140 (MQ=255)
                                                                                    aaTCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCt                                                                       >  4:46938/1‑124 (MQ=255)
                                                                                        cccgcATAAGTTAGAATGTTACCAGTCATCTTTTTATGGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTgg                                                   <  7:1025105/139‑1 (MQ=255)
                                                                                        cccgcAGAAGTTATCAAGTAACCATTAATATGTTTATAGCTAACAT‑AAAAACCGAGCCCGCAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTgg                                                   <  5:636445/139‑1 (MQ=255)
                                                                                         ccgcAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGc                                                  >  2:789235/1‑140 (MQ=255)
                                                                                         ccgcAGAAGTGAGCAAGTAACCCGTAAGCTGTTGATAGCTTTCAC‑TAAACCCGCTGCCGCAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGc                                                  <  1:495650/140‑1 (MQ=255)
                                                                                            ctgAAGTGCGCAAGTTCCCTGTCTTCTGTGTATCGCGTTCAC‑AAAACCCGCTGCCGGAAAAAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAAc                                               <  8:276706/138‑1 (MQ=255)
                                                                                                    agaatgTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAACCAAACGACTCCACGCAGTTGTGTTTGTCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATCGCCTGGCAACGTGatatt                                       >  5:223417/6‑139 (MQ=255)
                                                                                                    aGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGCCTGCTGGACCCTCTTCCTGCTCCGTATAGCCTGGCAACGTGATATg                                       >  1:1004190/1‑140 (MQ=255)
                                                                                                     gCAAGTTACAAGTCATGTGTTTATAGCGTTCAC‑TAAAACCGCTGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGatat                                        <  3:795561/138‑1 (MQ=255)
                                                                                                     ccAAGTATCCAGACATGTGTTTCTAGCG‑TCACATAAACCCGCGGCCGTAAACACACGACTCCACGAAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTTATATGa                                      <  7:32208/139‑1 (MQ=255)
                                                                                                          ttACAAGTCATCTGTTTATAGGGTTCAA‑TAAACCCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCg                                   <  3:152865/138‑1 (MQ=255)
                                                                                                          taacgaGTCATGTGTTGATATCTTTCAC‑TAAAACGGCTGCCGCAAACAAACGACTCCACGCAGTTGTGTTTTCCGCAATAGCAACTGACTGATTGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCa                                 <  7:711646/135‑1 (MQ=255)
                                                                                                           tACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAACCGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCCTCTTCCTCCTCCGTATAGCCTGGCAACGTGATATGACCGCAt                                >  2:175979/1‑140 (MQ=255)
                                                                                                           tACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGc                                  >  4:371924/1‑138 (MQ=255)
                                                                                                            aCCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCATCATGCTCCGTATAGCCTGGCAACGTGATATGACCGCAtt                               >  6:644350/1‑140 (MQ=255)
                                                                                                             ccAGTCATCTTTTGATAGCGTTAAA‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTc                              <  1:475089/140‑1 (MQ=255)
                                                                                                              cAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAact                                                                                       >  1:863458/1‑82 (MQ=255)
                                                                                                               aGTCATCTGTTTATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATGTGACCGCATTc                              <  4:900885/138‑1 (MQ=255)
                                                                                                                 tCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGCCTCCCCGCAGTTGTGTATGCCGCCATAGCAACTGACTGATGGCCCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgcc                          >  5:616816/1‑139 (MQ=255)
                                                                                                                 tCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgca                          >  5:5393/1‑140 (MQ=255)
                                                                                                                   aTCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCGACGTGATCTGACCGCATTCGgcagc                        >  5:980862/1‑140 (MQ=255)
                                                                                                                   aTCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgcagc                        >  1:381106/1‑140 (MQ=255)
                                                                                                                   aTCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATGCGgcagc                        >  6:131898/1‑140 (MQ=255)
                                                                                                                     gtgttgtTCGCGTACAA‑TAAAACCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCAt                      <  1:767835/139‑1 (MQ=255)
                                                                                                                     gtGTTTATAGCGTGCAC‑TAAAACCGCTCCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCAt                      <  7:217514/139‑1 (MQ=255)
                                                                                                                          taTAGCGTTCAC‑TAAAACCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTccc                 <  1:139669/139‑1 (MQ=255)
                                                                                                                          gATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTc                   >  4:681117/1‑138 (MQ=255)
                                                                                                                           aTAGAGTTCAC‑TTAAACCTCTACCGGAAAAAAACGACTCCACGCAGTTGTGATTGCCGCAATAGAAACTGACTGATGGACCATCTTCCTGCTCCTTATAGCCTTGCAACGTGATATGACAGCATTCGGCAGCATTTcccc                >  8:72805/1‑140 (MQ=255)
                                                                                                                             aGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAg              >  1:1028226/1‑140 (MQ=255)
                                                                                                                             aGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAg              >  1:110236/1‑140 (MQ=255)
                                                                                                                              gCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg             <  1:626279/140‑1 (MQ=255)
                                                                                                                              gCGTTCAC‑TAAAACCGCGGCCGGAACCAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATTGACCATCTTCCTGCTCCTTATAGCCTGGCAACGTGATATGACAGCATTCGGCAGCATTTCCCCAgg             <  8:1040904/140‑1 (MQ=255)
                                                                                                                              gCGTTCAA‑TAAAACCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg             <  5:861818/140‑1 (MQ=255)
                                                                                                                               cGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGGTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAg              >  4:603090/1‑138 (MQ=255)
                                                                                                                               cGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAg              >  4:1067576/1‑138 (MQ=255)
                                                                                                                               cGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGc            >  5:287714/1‑140 (MQ=255)
                                                                                                                               cGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGAGATATGACCGCATTCGGCAGCATTTCCCCAGGc            >  6:332443/1‑140 (MQ=255)
                                                                                                                                 ttCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATCGACCATCTTCCTGCTCCGTATAGCCTGGCAACCTGATATGACCGCATTCTGCCGCATTTCCCCACGc            >  3:36639/1‑138 (MQ=255)
                                                                                                                                  tCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGcccc         <  1:258102/140‑1 (MQ=255)
                                                                                                                                  tCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGcccc         >  8:893855/1‑140 (MQ=255)
                                                                                                                                  tCAA‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg             <  2:563798/136‑1 (MQ=255)
                                                                                                                                          aaCCGCTGCCGGAAACAAACGACTCCAAGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCCTCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAAc  >  1:946008/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GGTTGCCAGCGTGCGGGCAAGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAAC  >  NZ_CP022686/2572567‑2572843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A