Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F36 I0 R1 179 8076.5 8499890 98.3% 8355391 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,479,607 A→C 16.3% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,479,6070AC16.3% 368.8 / 21.7 203intergenic (‑16/+177)ftsB/ygbEcell division protein FtsB/DUF3561 family protein
Reads supporting (aligned to +/- strand):  ref base A (118/44);  new base C (2/36);  total (122/81)
Fisher's exact test for biased strand distribution p-value = 4.30e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

CTTGCTGTGCCGCCACATCATTATTGACGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTT  >  NZ_CP022686/1479471‑1479744
                                                                                                                                         |                                                                                                                                          
cTTGCTGTGCCGCCACATCATTATTGACGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACgg                                                                                                                                         <  3:141376/140‑1 (MQ=255)
    cTGTGCCGCCACATCATTATTGACGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑a                                                                                                                                      >  8:753666/1‑138 (MQ=255)
    cTGTGCCGCCACATCATTATTGACGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATc                                                                                                                                    >  2:1073160/1‑140 (MQ=255)
    cTGTGCAGCCACATCATTATTGACGAGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATc                                                                                                                                    <  1:381983/140‑1 (MQ=255)
       tgCCGCCACATCATTATTGACGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTc                                                                                                                                 <  3:569393/140‑1 (MQ=255)
          cgccACATCATTATTGACGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATc                                                                                                                              <  2:778198/140‑1 (MQ=255)
            ccACATCATTATTGACGCGAGAATAGTCATGTAAACCGTTCTTACCGAACCACACCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTAAGTTTTCCC‑ATCCTGCCCCCTGAAACACGCC‑AACTTCATacc                                                                                                                            >  4:702305/1‑140 (MQ=255)
             cacaTCATTATTGACGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATcc                                                                                                                             <  7:515783/138‑1 (MQ=255)
                         gACGCTAGTATTGTCATGTATACCGTTATTACCTACCCACACCTAATACTGTCGCCAGACCAGAATCGCCAGCAACAGCAGGTTTAGTTTACCC‑ATCCTGCCCCCTGAAAACCGGC‑ATCTTAATCCCATGCATCCGAAga                                                                                                               <  4:762826/140‑1 (MQ=255)
                          aCGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAgcc                                                                                                              >  2:148577/1‑138 (MQ=255)
                          aCGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCA‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAgac                                                                                                              >  2:556508/1‑140 (MQ=255)
                          aCGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACACCCAATACTGTAGACAGACCAGAATAGCCAGCAACCGCAGCGTAAGTTTACCC‑ATCCAGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGAATCCGAAgac                                                                                                              >  6:585939/1‑140 (MQ=255)
                           cgccAGTATAGTCTCGTATACCGTACTTACCCACCCACAGCTAATAAGTTAGCCATACCAGAATAGCCAGCAACAGCCGCTTTTTTTTCACC‑ATCCCGCCCCCTTAAAAACGGC‑ATCTTCATCCCATGCATCCGAAgacg                                                                                                             <  2:689867/140‑1 (MQ=255)
                            gcgAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAgacga                                                                                                            >  3:114880/1‑140 (MQ=255)
                            gcgAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAgacga                                                                                                            >  3:302705/1‑140 (MQ=255)
                            gcgAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAgac                                                                                                              >  7:434395/1‑138 (MQ=255)
                               aGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATACTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGActc                                                                                                         >  5:177230/1‑140 (MQ=255)
                               aGTATAGTCATGTATACCGTTCTTACAGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGA‑ATCTTAATCCAATGCATCCGAAGACGActc                                                                                                         >  3:427980/1‑140 (MQ=255)
                                       aatttataCCGTTCTTACCGAACCACACCTAATACTCTAGCCACACCAGAATAGCCCCCAACCGCAGCGTTAGTTACCCC‑AGCCTGCCCCCTCAAAAACGGC‑ATCTTCATCCCATGCACCCCACCACGACTCTACATCct                                                                                                 <  6:477521/136‑1 (MQ=255)
                                       aaTGTATACCGTTCTTACCGAACCACAGCCAATAGTTTCGCCACCCCACAATCGCCAGCAACCGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTTAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCct                                                                                                 <  4:741364/139‑1 (MQ=255)
                                         tGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGCCTCTACATCctct                                                                                               >  6:432485/1‑140 (MQ=255)
                                          gTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTg                                                                                              >  6:104106/1‑140 (MQ=255)
                                          gTATACCGTTCTTACCGAACAACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATACTGCCCCCTGATAAACCGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACCTCCTCTg                                                                                              >  4:927582/1‑140 (MQ=255)
                                           tatcCCGATCATACCGCCCCACAGCGAATACAGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTGAGTTTACCC‑AGCCTCCCCCCAAAAAAACGGC‑ATCTTCATAGCATGCATCCGAAGACGACTCTACAACCTCTGt                                                                                             >  2:987344/1‑140 (MQ=255)
                                           tataCCGTTGTAACCCACCCACCGCTAATACTGTAGCAAGACCATAATAGCCTGCAACAGCAGCGTTTGTTAACCC‑AACCTGCCCCCTCAAAACCGCA‑TTCTTCATCCAATGCATCCGAACACGACTCTACATCCTCTGt                                                                                             <  4:500651/140‑1 (MQ=255)
                                            ataCCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCCTCCGAAGACGACTCTACATCCACTg                                                                                              >  8:825506/1‑138 (MQ=255)
                                              aCCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACAGCCTCTGTTgg                                                                                          >  6:249144/1‑140 (MQ=255)
                                               ccGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTg                                                                                           >  7:393646/1‑138 (MQ=255)
                                                cGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTgggg                                                                                        >  6:309953/1‑140 (MQ=255)
                                                  ttCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTACATCCCATGCATCCGAAGACGACTATACATCCTCTGTTgggg                                                                                        >  8:882298/1‑138 (MQ=255)
                                                   tCTTACCGAACCCCTGCGAATCGTGTCGCCAGCCCATAATACCCCTCACCAGCTGCGTTTTTTTACCC‑ACCGTCCCCCCTGAAACCCGTC‑AGCTACACCCCAGCCAGCCGACGACGACTCTACATCCTCTGTTGGGGATa                                                                                     <  2:455553/140‑1 (MQ=255)
                                                    cTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGCCGACTCTACATCCTCTGTTGGGGAt                                                                                      >  7:648149/1‑138 (MQ=255)
                                                    cTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                      >  8:642333/1‑138 (MQ=255)
                                                    cTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                      >  7:346537/1‑138 (MQ=255)
                                                    cTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                      >  7:838212/1‑138 (MQ=255)
                                                    cTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                      >  7:364880/1‑138 (MQ=255)
                                                    cTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                      >  7:77274/1‑138 (MQ=255)
                                                    cTTACCGAACCACAGCGAATACTGTAGACAGACCAGAATAGCCAGCAACAGCAGAGTTAGTTTACAC‑ATCCTGCCCCATGAAAATCGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTGTGTTGGGGATAc                                                                                    >  2:693500/1‑140 (MQ=255)
                                                       aCCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑CTCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACcgc                                                                                 >  3:881005/1‑140 (MQ=255)
                                                       aCCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACagc                                                                                 >  3:490189/1‑140 (MQ=255)
                                                       aCCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGGTGGGGATACcgc                                                                                 >  6:679427/1‑140 (MQ=255)
                                                         cGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACcgc                                                                                 >  7:47429/1‑138 (MQ=255)
                                                          gAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGAc                                                                              >  1:483135/1‑140 (MQ=255)
                                                          gAACCACAGCGAATACTGTAGCCACACCAGAATAGCCAGCACCAGCAGCGTTAGTTTACCC‑ATCCAGCCCCCTGAAAAACGGC‑ATATTCATCCCATGCATCCGAAGACGCCTCTACATACAATGATGGGGATAcctccac                                                                              >  1:151973/1‑135 (MQ=255)
                                                            aCCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCCCGACaa                                                                            >  4:577223/1‑140 (MQ=255)
                                                              caccccGAATACTGTACCCACACCCGAACCCCCACCAACACCCGCGTTAGTTACCCC‑ATCCGCCCCCCTCAAAACCGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAAcg                                                                          <  4:505016/135‑1 (MQ=255)
                                                                caGCCAAAACTTTACCCAGCCCAGAAAACCCCGAAACCGCAGCGTTTGTTAACCC‑ACCCTCCCCCCTTAAACCCGGC‑ATCTTCATCCCATCCATCCCACGACGACTCTACATCCTCTGTTGGGGATACCGCGACAAcgcg                                                                        <  6:831446/140‑1 (MQ=255)
                                                                   cGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGc                                                                     >  3:951988/1‑140 (MQ=255)
                                                                     aaTACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCCTCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCaa                                                                   >  2:487288/1‑140 (MQ=255)
                                                                        aCTGTACCCCCACCAGAACACCCCCAAACAGCAGGGTTCTTTTCCCC‑ATCC‑GCCCCCCGCAAACCCGCAAGCTTCATCCCATCCACCCGACGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATg                                                                <  4:475521/140‑1 (MQ=255)
                                                                           gtccccagcccacaAAAGCCAGCACCAGCTGCTTTAGTTTCCCC‑ATCCTGCCCCCTCAAAACCGCC‑ATATGACTCCCCTGCACCCGAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGt                                                               <  7:712630/134‑1 (MQ=255)
                                                                           gTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTAc                                                             >  6:889227/1‑140 (MQ=255)
                                                                           gTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTAc                                                             >  3:521326/1‑140 (MQ=255)
                                                                           gTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCGATTGGGGATACAGCGACAACGCGGGCAAATGTAc                                                             >  5:528814/1‑140 (MQ=255)
                                                                           gTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCATCTGTTGGGGATCCCGCGACAACGCGGGCAAATGTAc                                                             >  5:890217/1‑140 (MQ=255)
                                                                           gTAGCCAGACAAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATACTCTGTTGGGGATACCGCGACAAAGCGGGCAAATGTAc                                                             >  1:350843/1‑140 (MQ=255)
                                                                             ccccagaccagaATCGCCCGCAACAGCCGCGTTTGTATACCC‑ATCCTCCCCCCTTAAAACCGCC‑ATCTGCAACCCATCCACCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTAc                                                             <  8:940254/136‑1 (MQ=255)
                                                                             aGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGaa                                                                                                                 <  8:510456/86‑1 (MQ=255)
                                                                                  gaccagaATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACAttt                                                      >  5:709619/1‑140 (MQ=255)
                                                                                   accagaATAGCCAGCAACAGCAGCGTTAGTTTACCC‑CTCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATCCCGCGACAACGCGGGCAAATGTACCACATTTg                                                     >  3:81243/1‑140 (MQ=255)
                                                                                   accagaATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGTCAAATGTACCACATTTg                                                     >  4:300992/1‑140 (MQ=255)
                                                                                   accagaATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTg                                                     >  3:613376/1‑140 (MQ=255)
                                                                                    ccagaATAGCCAGCAACCCCAGCGTTAGTTTACCC‑ATCCTGCCCCCTTAAAAACGCC‑TTCTTCACCCCATCCACCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGt                                                    <  3:321193/140‑1 (MQ=255)
                                                                                     cagaATAGCCAGCAACAGCAGCGGTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCCTCAGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTg                                                     >  8:186365/1‑138 (MQ=255)
                                                                                           cgccacaaaCAGCCGGGTTCGTTAACCC‑ATCCTCCCCCCTGAAAACCGCC‑ATCTTCATCCAAGTCACCCGACCACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGt                                             <  6:766043/133‑1 (MQ=255)
                                                                                           cgCCAGCAACAGCAGCTTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGCC‑ATCTTCATCCAATGCATCCTAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGt                                             <  4:611632/139‑1 (MQ=255)
                                                                                           cgCCAACAACTGCCGCGTTA‑TTAACCCAAACCTTCCCCCTCAAACCCGGA‑AACTAAATCCAAGGCAGCCTACGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGAtt                                               <  7:1006788/137‑1 (MQ=255)
                                                                                           ccccaccaACACCAGCGTTAGTTACCCC‑ATCATGCCCCATTAAAAAGGGC‑ATCTTCATCCCATGAACCCGCCGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGAtt                                               <  7:909610/136‑1 (MQ=255)
                                                                                           ccccAGCAACCTCTGCTTTAGTTAACCC‑ACCCCCCCCCCTTAAAAACGCA‑CTCTTCATCCATTGCATCATATCACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGt                                             <  5:260530/138‑1 (MQ=255)
                                                                                           agcaagcaCCAGCAGCGTTAGTTTCCCC‑CTCCTGCCCCCTTAAAACCGCC‑ATATTCATCCCATGCACCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGt                                             <  2:239437/140‑1 (MQ=255)
                                                                                               agaaaCAGCAGCGTTATTTAACCC‑ACCCTCCCCCCTGAAAAAGGCA‑ATCTTAATCCCATGCATCCTAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACg                                         <  4:867361/137‑1 (MQ=255)
                                                                                               accAACAGCAGCGTTATTTAACCC‑ATCCTGCCCCATGAAAAACGCC‑ATCTTCATCCCATGCCTCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACg                                         <  4:244686/138‑1 (MQ=255)
                                                                                               accAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTTAAAACCGGC‑ATCTTCACCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACg                                         <  5:321293/138‑1 (MQ=255)
                                                                                               aGCAACCGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAAGGGC‑ATCTTCATCCCATGCATCCTAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACg                                         <  4:797695/140‑1 (MQ=255)
                                                                                               aGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGCCTCTACATCCGCTGTTGGGGATACCGCGACACCGCGGGCAAATGTACCACAGTTGTCGATTGTTa                                           >  8:732613/1‑138 (MQ=255)
                                                                                               aGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTa                                           >  8:481941/1‑138 (MQ=255)
                                                                                               aGCAACACCAGCTTTAGTTTACCC‑ATCCTCCCCCCTAAAAACCGGC‑ATCTACAACCCATGCATCCGACCACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACg                                         <  1:332639/140‑1 (MQ=255)
                                                                                                 cAACAGCCGCTTTAGTTTACCA‑ATCCTGCCCCCTTAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGta                                       <  4:1017290/140‑1 (MQ=255)
                                                                                                       cagcTTTAGTTACCCC‑ATCCTCCCCCCTCAAAACCGGA‑ATATTCAACCCATGAACCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCa                                 <  6:740889/140‑1 (MQ=255)
                                                                                                       cagcTTTAGTTAACCC‑ATCCTGCCCCCTTAAAAACGGC‑ATATTCAACCCATGCACCCGACGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCa                                 <  4:536113/140‑1 (MQ=255)
                                                                                                         gcGTTATTTTACCC‑ATCCTTCCCCCTGAAACACGGC‑ATCTTCATACAATGCATCCTAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAgg                               <  2:888924/140‑1 (MQ=255)
                                                                                                         gcGTTAGTTTACCC‑ATCCTGCCCCCTTAAAAACGGA‑ATATTCAACCCTTGCATCCGACCACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCa                                 <  8:353306/138‑1 (MQ=255)
                                                                                                           gTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGc                             >  3:185316/1‑140 (MQ=255)
                                                                                                            ttAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCg                            >  5:100870/1‑140 (MQ=255)
                                                                                                              tgTTTACCC‑ATCCTGCCCCCTCAAAACCGTC‑ATCGTCAGCCCATGCCTCCCAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCg                            <  8:694985/137‑1 (MQ=255)
                                                                                                              tgTTTACCC‑ATCCTCCCCCCTTAAAACCGCC‑TTATCCACCCCATGCATCCCATGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTg                          <  4:185316/139‑1 (MQ=255)
                                                                                                              tgTTAACCC‑ATCCTGCCCCCTTAAAAACGGC‑AGCTTCATCCCATGCAGCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCg                            <  7:250609/137‑1 (MQ=255)
                                                                                                              aGTTTCCCC‑AGCCACCCCCCTTAAAACCGCC‑ATCTTAAACCCAGCCATCCGACGACGACTATACATCCTCTGTTTGGGATACCGCGACAACGAGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTg                          <  5:597446/140‑1 (MQ=255)
                                                                                                               ttttACCA‑ATCTTGCCCACTTAAAACCGTA‑ATTTTAATCCCATGAAGCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGt                           <  7:593600/137‑1 (MQ=255)
                                                                                                                tttACCC‑ATCCTGCCCCCTCAAAACCGGC‑ATCTCCAACCCATGCATCCTAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGTGCGTGCa                        <  2:379696/140‑1 (MQ=255)
                                                                                                                tttACCC‑ACCCGCCCCCCTCAAAACCGGC‑ATCTTCTACCAATGCCTCCGAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCa                        <  2:1015986/140‑1 (MQ=255)
                                                                                                                  tACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTAGATTGTTACGTATACCCAGGGCGTGCAGa                      >  1:883163/1‑140 (MQ=255)
                                                                                                                    ccc‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAAc                    >  2:580905/1‑140 (MQ=255)
                                                                                                                    ccc‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCATCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACTTATAACCAGGGCGTGCAGAAc                    >  1:780537/1‑140 (MQ=255)
                                                                                                                      c‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGCCTCTACATCCTCTGTTGGGGATACCGCTACGACGCGGGCAATTGTACAACATTAGTAGATTGTTACGTATAACAATGGCGTGAAGAAc                    >  8:318339/1‑138 (MQ=255)
                                                                                                                          ccaccccacTTAAAACCGGC‑ATCTTCATCCCATGCAGCCGAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAAt               <  5:836448/136‑1 (MQ=255)
                                                                                                                          ccTCCCCCCTCAAAAACGCC‑ATCATCATCCCAGGCAACCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAAt               <  4:990999/140‑1 (MQ=255)
                                                                                                                            tGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGTCAACGCGGGCACATGTCCCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAAt               >  8:633249/1‑138 (MQ=255)
                                                                                                                                    gAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCattat       >  8:895212/1‑138 (MQ=255)
                                                                                                                                      aaaaCGCC‑AACTACAACCCATCCAACCTAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGt   <  1:1073160/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CTTGCTGTGCCGCCACATCATTATTGACGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGC‑ATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTT  >  NZ_CP022686/1479471‑1479744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A