Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F2 I0 R1 74 6379.7 5468672 98.4% 5381173 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,244 T→G 12.0% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2440TG12.0% 191.1 / 3.4 122T182P (ACC→CCC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (29/75);  new base G (10/8);  total (39/83)
Fisher's exact test for biased strand distribution p-value = 2.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

CGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAT  >  NZ_CP022686/1674106‑1674381
                                                                                                                                            |                                                                                                                                         
cgcgGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTTTACGTCTGGCGcggtg                                                                                                                                          >  3:9050/1‑139 (MQ=255)
  cggcgCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑GT‑TTGTTCGTGTGGCgcgggcgg                                                                                                                                        >  1:327679/1‑140 (MQ=255)
   ggcgCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGcggtcggt                                                                                                                                       <  1:9232/140‑1 (MQ=255)
     cgcTGCCGGGTTGCATTTGCTCTACAAGTGCATGACGGCGACGCTGAAACTCTAGACTGTATTTATCACTCTTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCTGT‑TT‑TTGTACTTCTGGCGCGGTCTGTTg                                                                                                                                     <  5:419779/140‑1 (MQ=255)
        tGCCGGGTTGCATTTGCTCTACCATGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                  <  1:687282/140‑1 (MQ=255)
        tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                  <  4:429892/140‑1 (MQ=255)
        tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                  <  6:37724/140‑1 (MQ=255)
        tGCCGGGGTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGc                                                                                                                                    <  8:424349/138‑1 (MQ=255)
        ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGGCGGTGGCgg                                                                                                                                  <  6:145523/139‑1 (MQ=255)
         gCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGa                                                                                                                                 <  5:100343/140‑1 (MQ=255)
          ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                  <  7:162729/138‑1 (MQ=255)
          ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                                                <  5:512861/140‑1 (MQ=255)
          ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                                                >  4:441918/1‑140 (MQ=255)
               ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTATGGCGCGGTCGGGTGCGGATGAGtt                                                                                                                           >  3:435515/1‑139 (MQ=255)
                  cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTaa                                                                                                                          <  8:397173/138‑1 (MQ=255)
                  cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTCTTGCGCGGTCGGTTTCGGATGAGTaaaa                                                                                                                        >  5:360355/1‑139 (MQ=255)
                  cATTTGCTCTACCAGGGCCGGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAg                                                                                                                        <  4:688951/140‑1 (MQ=255)
                      tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTATAGgtgt                                                                                                                    <  2:398098/140‑1 (MQ=255)
                      tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                    <  3:478703/140‑1 (MQ=255)
                      tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                    <  3:441918/140‑1 (MQ=255)
                      tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                    <  5:641224/140‑1 (MQ=255)
                      tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                    <  4:212939/140‑1 (MQ=255)
                      tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                    <  4:424193/140‑1 (MQ=255)
                      tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                    <  6:581319/140‑1 (MQ=255)
                      tGCTCTACCAGGGCCTGAAGTCGACGCTGAAACTCTTGCCGGTATATATCACTCATAACACTCTCCTTACATATTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTCCTTCTGGCGCGTTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                    <  5:89572/140‑1 (MQ=255)
                        gtctACCAGGGCCTGACGGCGACGCTGTAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                  <  1:398246/139‑1 (MQ=255)
                        ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATGACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                  <  5:44724/140‑1 (MQ=255)
                        ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                    <  7:104973/138‑1 (MQ=255)
                        ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                    <  7:562090/138‑1 (MQ=255)
                            aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTCTGGCGCGGTGGGGTGCGGGTGGTGTAAGGTTTCGTggg                                                                                                              >  3:334697/1‑139 (MQ=255)
                            aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTACTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGTGGCGGGCCGGTGAGGAATAGTTAAGGTGTCGTGGc                                                                                                              >  6:538662/1‑140 (MQ=255)
                              cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCAt                                                                                                            <  6:400643/140‑1 (MQ=255)
                                  gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑GT‑TTGTACTTATGGCGCGGGAGGTTGCGGATGAGTTTAGGTGTCGTGGGATAACa                                                                                                        >  4:640288/1‑140 (MQ=255)
                                  gCCAGACGGCGACGCTGAAAATCTTGCCGGGATATATCACTCATACCACTCTCCTTACATTTTTTGTATTTAGTGTATAGTCGGTGTT‑TTGT‑CATGTGGCGCGGGCGGTTGGGGATGAGGAAAGGGGTCGGGGCAGAAcc                                                                                                        >  4:49774/1‑139 (MQ=255)
                                   ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg                                                                                                       <  1:602651/140‑1 (MQ=255)
                                   ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg                                                                                                       <  2:347713/140‑1 (MQ=255)
                                     tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTTTTATTGAGGCGCGGGCGGGTGGGGGTTATTAAAGGGGTGGGGGGATAACAgcg                                                                                                     >  2:45535/1‑140 (MQ=255)
                                     tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTTTAATTGTTGGGCGGTCGGTTGCGGATTAGTAAAGGTGTGGTGGGATAACAgcg                                                                                                     >  2:201211/1‑140 (MQ=255)
                                     tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTATGGAGGGGGGGGGTGCGGTTGAGTTAATGTGGAGTGGGAAAACAccg                                                                                                     >  1:65747/1‑140 (MQ=255)
                                       aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTGTTGT‑GTTGTGGCGCGGGGGGGTGCGGATGGGGAAAGGGGGCGGGGCATAACAGcgcg                                                                                                   >  3:349264/1‑140 (MQ=255)
                                       aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTTGTTCTGGGGCGGTCGGTTGGGGATGAGTTAAGGTGTGGTGGCATAAAAGcgcg                                                                                                   >  6:331064/1‑140 (MQ=255)
                                       aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTTCTTATTGCGCGGTCGGTTGAGGATGAGTTAAGGTGTAGTGGGCTAACAGcgcg                                                                                                   >  1:82137/1‑140 (MQ=255)
                                       aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTCTGGCGCGGTCGGGTGCGGATGAGGAAAGGTGTGGTGGCATATCAGagcg                                                                                                   >  2:278064/1‑140 (MQ=255)
                                        cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                                                  <  2:405227/140‑1 (MQ=255)
                                        cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGATTTTAGTGAAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGGTGCGGAGGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                                                  <  1:220624/140‑1 (MQ=255)
                                         ggCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGcgcg                                                                                                   >  8:4800/1‑138 (MQ=255)
                                         ggCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGc                                                                                                 <  4:11769/140‑1 (MQ=255)
                                            gACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTATGGCGCGGTCGGTTGCGGATGTGTTAAGGTGTGGTGGCATAACAGCGCGGCAac                                                                                              >  2:375347/1‑140 (MQ=255)
                                             aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTATAGTAGGG‑TT‑TTGTTCTTCTGGCGCGGTCGGGTGCGGATTATTAAAGGTTTCGTGGGCTAACAGCGCGGCAaca                                                                                             >  2:503392/1‑140 (MQ=255)
                                             aCGCTGAAACTCTAGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTTCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                             <  1:132547/140‑1 (MQ=255)
                                                  gAAACTATTGCCGGGATATATCACTCATACCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGA‑TT‑TTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGAc                                                                                        <  6:652223/140‑1 (MQ=255)
                                                         ttGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTTTTCTTATGGGGCGGTCCGTTGCGGATGTGTTAATGTGTCGTGGCATAAACCCGCGGCAACACCGACGTaaacg                                                                                 >  5:391720/1‑136 (MQ=255)
                                                           gCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTTTTCTTCTGGCGCGGGCGGTTGCGGATGAGTAAATGTGTCGTGGCATAACACCGCGGCCACACCGACGTAATCgat                                                                               >  3:341913/1‑140 (MQ=255)
                                                            ccGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCga                                                                                <  7:4800/138‑1 (MQ=255)
                                                              gggCTATATCACCCAAAACCCCAACCTTACAATTTTTTTTTTTATGGTAGTCTCCGG‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                            <  1:368382/140‑1 (MQ=255)
                                                              gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCTTCAACACGGACGTACTCgatgat                                                                            <  5:671676/140‑1 (MQ=255)
                                                              gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                            <  6:99163/140‑1 (MQ=255)
                                                              gggATATAGCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                            <  4:58470/140‑1 (MQ=255)
                                                                         cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGGACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                                                 <  2:411072/140‑1 (MQ=255)
                                                                          tCATATCACTCTCCTTAGATTTTTTGTTTTTAGTGTAGAGTCGGT‑TG‑TTGTTCTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                <  3:235856/140‑1 (MQ=255)
                                                                          tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                <  1:509599/140‑1 (MQ=255)
                                                                          tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                <  6:409208/140‑1 (MQ=255)
                                                                          tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTATGTCGCGGGCGGTTGGGGGTGTGGTAAGGTGGCGTGGCATAACAGCGCGGGACCACGGCCGTACTGGATGAGCTCTTCAAGCgg                                                                >  2:230052/1‑139 (MQ=255)
                                                                          tCATAACACTCTCCTTACATTTTTTGTTATTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGGGTCGTGGCAAAACAGCGCGGCAACACGGACCGACACGATGATCTCATCaaaccc                                                                >  4:295179/1‑136 (MQ=255)
                                                                                    ctcCTTACCTTTTTTGTTTTTCGGGTAGAGGCGGG‑TT‑TTGTACTGCTGGCGCGGTCGGTTGCGGATGAGTACAGGTGGCGGGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACTTTTCAAGc                                                      <  2:193430/140‑1 (MQ=255)
                                                                                    ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTTTACTTATTGCGCGGGCGGTTGAGGATGAGTAAATGGGTCGTGGCATAACAGCGCGGGCAAACCGAAGTACTCGATGATATCTTAAATCGACATTTaaatc                                                      >  4:160080/1‑138 (MQ=255)
                                                                                    ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTTTAATTATAGCGCGGTGGGTTGCTGAAGATAAAATGTGTGGTGGCCTAACAGCGCGGCCACAACGACCTTAACGATGATCTAATGAAGCCACATTTaaatc                                                      >  6:93399/1‑138 (MQ=255)
                                                                                    ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                      <  2:151490/140‑1 (MQ=255)
                                                                                    ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTAATTATGGCGCGATACGTTGAGGATGAGTAAAGGTGTGGTGGCATAACAGCGCGGCAACACCGACGGGCTCGCTGATCTCTTCAAGAGACATTTCAAGc                                                      >  4:517341/1‑140 (MQ=255)
                                                                                     tacTTACATTTATTGTTTTTAGTGTAGAGTCTGT‑GT‑ATGTACTTCAGGCGCGGTCGGTTGCGGATGAGTAAAGGGGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTAAAGCGACATTTCAAGct                                                     <  3:681374/138‑1 (MQ=255)
                                                                                      ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTAGTTCTGGCGGGGTCGGTTGCGGATGAGTAAATGTGTAGTGGGATAACAGCGCGGCAACACGGACGTACTCGATGATATCTTAAAGCGCCATTTAAAgg                                                      >  8:306525/1‑137 (MQ=255)
                                                                                      ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGGGCGGTGGGTTGCGGATTAGTTAAGGTGTCGTGGCATAAACGCGCGGCAACACGGACGTACTAGAAGATCTATTAAAGCGACAATTaaaccaa                                                    >  2:687518/1‑136 (MQ=255)
                                                                                       cTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACATATGGCGCCGTCGGTTGCGGAAGAGTAAAGGAGTCGGGGGATAACACCCCGGCAACACCGACGGACTAGAACATCAAATGAAGCGACATTTAAAggg                                                     >  8:612662/1‑136 (MQ=255)
                                                                                          aCATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                                                <  3:413802/140‑1 (MQ=255)
                                                                                          aCATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTAATTAAGGCGCTCTCGGTTGCGGAAGAGTTAAGGTGTCGTGGCAAATCACAGCCGCCACACGGACGTAGACGATGATCTCTTCAACCCACATTTAAAGatcgtac                                                >  6:179905/1‑136 (MQ=255)
                                                                                                       ttAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt                                   <  1:538002/140‑1 (MQ=255)
                                                                                                          ttgtAGAGTCGGT‑TT‑TTGTTCTTCTGGCGCGGTCGGTTGCGGATTAGTAAAGGAGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                <  5:426567/139‑1 (MQ=255)
                                                                                                            gtAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                <  7:228487/138‑1 (MQ=255)
                                                                                                            gtAGAGTCGGT‑TT‑TTGTACTGCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                <  7:613535/138‑1 (MQ=255)
                                                                                                                agTCGGG‑TT‑TTGGACATATGGGGCGGTGGGTTGCGGATGAGGAAAGGTGTCGTGGCATAACAGCGCGGCAAAAAGGCCGGACTCGATGAACACTTCAAGCGCAATTTAAAGCAGCTCCGGATCTACGTCATAGTGGTAAc                          >  4:665085/1‑140 (MQ=255)
                                                                                                                    ggT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa                      <  3:282254/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCTGTCGGTTGCGGATGATTAAAGGTGTCGGTGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGAAATTTCAATCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                 <  1:325627/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                 <  2:545672/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                 <  3:685640/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAGCTTCGTCTTCGTCGTAACCCAACTgcg                 <  2:499468/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                 <  5:4246/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGGCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                 <  5:352212/140‑1 (MQ=255)
                                                                                                                            tGTACTTATGGCGCGTTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGAAGTAAACGAAGATCTCTTAAAGCGACAATTCAAGCTCTTCCAGATCTTCGACTTCGTCGTAACCACACAgcgc                >  3:182399/1‑140 (MQ=255)
                                                                                                                             gTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                 <  7:491211/138‑1 (MQ=255)
                                                                                                                             gTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg               <  1:438015/140‑1 (MQ=255)
                                                                                                                             gTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGGCGTAACCCAACTGcgcg               <  2:270697/140‑1 (MQ=255)
                                                                                                                                cTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATg            <  3:5077/140‑1 (MQ=255)
                                                                                                                                     ggCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAATCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACg       <  4:535136/140‑1 (MQ=255)
                                                                                                                                     ggCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTa         <  7:569013/138‑1 (MQ=255)
                                                                                                                                     ggCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTa         <  7:572099/138‑1 (MQ=255)
                                                                                                                                      gcgcGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGc      >  4:329488/1‑140 (MQ=255)
                                                                                                                                        gcgGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGAATATATCTTCAAGCGACATTTCAATCTCTTCCTGATCTTCGTATTCGTCGTAACCCAACTGCTCGATGTTACga      >  8:536168/1‑137 (MQ=255)
                                                                                                                                         cggtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa   <  2:631478/140‑1 (MQ=255)
                                                                                                                                         cggtcgggTGCGTATGAGTAAATGGTTCTTGGCAGAACTGCGCGGCAAAACGGACGTACTCGATGATCTCTTCAAGCGACATTTCCAGCTCTTCCTGCTCTTCGTCTTCTTCGTAACCCAACTGCGCGATGTTACGCAGa   <  4:158598/140‑1 (MQ=255)
                                                                                                                                          ggtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAt  >  2:585635/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                         
CGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAT  >  NZ_CP022686/1674106‑1674381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A