Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F35 I0 R1 174 9808.2 8488800 98.4% 8352979 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,244 T→G 10.1% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2440TG10.1% 317.6 / 6.8 180T182P (ACC→CCC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (46/111);  new base G (14/9);  total (60/120)
Fisher's exact test for biased strand distribution p-value = 4.18e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

GCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATC  >  NZ_CP022686/1674105‑1674382
                                                                                                                                            |                                                                                                                                          
gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGcggt                                                                                                                                            <  8:530268/140‑1 (MQ=255)
gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGcggt                                                                                                                                            <  8:973097/140‑1 (MQ=255)
   cggcgCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGcggtc                                                                                                                                           <  1:90913/138‑1 (MQ=255)
      cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTTCATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTATGGGGCGGTGGGTTg                                                                                                                                      >  5:910728/1‑140 (MQ=255)
      cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTTCTTATGGGGggggcggggg                                                                                                                                      >  7:568440/1‑137 (MQ=255)
      cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GGTTTGTTCTTCGGGGGCGGTCGGGTg                                                                                                                                      >  3:453922/1‑140 (MQ=255)
      cgcTGACGGGTTGCATTTGCTCTACCCGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACGCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTTCGTCTGTCGCGGTCGGGTg                                                                                                                                      >  4:831951/1‑140 (MQ=255)
         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                   <  5:17361/140‑1 (MQ=255)
         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                   <  3:774282/140‑1 (MQ=255)
         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGGCGGTTGCgg                                                                                                                                   <  8:701273/140‑1 (MQ=255)
         ggCCGTGTTGCATTTTCGCTACCAGGGCCTGACGGCGAAGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                   <  7:586288/139‑1 (MQ=255)
           ctgggTTGCATTTTCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCTT‑TTTTTGTACTTCTGGCGCGGTCGGTTggtgat                                                                                                                                 <  8:383372/138‑6 (MQ=255)
           ccGGGTTGCATTTGATCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACTCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGac                                                                                                                                 <  6:986875/140‑2 (MQ=255)
                ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACA‑‑‑‑‑‑‑TTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  6:157279/140‑1 (MQ=255)
                   cATTTGCTCTCCCAGGGCCGGACGGCGACGCGGACACTCGTGCCGGGATCTATCACTCATAACACGCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTCATACTTGCGCGGTCGGTGGCGGATGAGGAAAg                                                                                                                         <  8:643388/140‑1 (MQ=255)
                   cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTTCTTGTGGCGCGGGGGGGTGGGGGTGAGTTAAg                                                                                                                         >  8:458919/1‑140 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  4:296433/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  4:159981/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  5:485931/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  8:291566/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGATGAAACTCTTGACGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  5:967107/140‑1 (MQ=255)
                        gCTCTACCAGGGCCTGACGGCGACGCTGACACTCTTGCCGGGATATATCACTCATAACACTCTCATTACATTTTTTGTTTTTAGTGTAGAGTCTG‑GTTTATTACTTCTGGAGCGGGCGGTATCGGATGATTaaaagaggg                                                                                                                    >  4:804160/1‑134 (MQ=255)
                         ctctACCAGGGCCTGACGGCGTCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                   <  8:574960/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  2:713632/138‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  2:415352/138‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                   <  8:59329/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATAGCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                   <  6:537055/140‑1 (MQ=255)
                            tACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCATACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTgg                                                                                                                <  6:638354/140‑1 (MQ=255)
                             aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTTCTTCTGGCGCGGTCGGTTGCGGGTGAGTTAAGGTGTCGTggg                                                                                                               >  3:1015980/1‑139 (MQ=255)
                              ccAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTTTAATTATGGGGCGGTCGGTTGGGGATGAGTAAATGTGTGGTGGCa                                                                                                              >  3:860454/1‑140 (MQ=255)
                               cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTTTGCGTCTGGCGCGGGCGGTTGGGGGTGAGTAAAGGTGGCggtgg                                                                                                               >  2:736940/1‑134 (MQ=255)
                                aGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTAATTCTGGCGCGGTCGGTTGCGGATGAGTTAATGTGTCGTGGcg                                                                                                              >  1:625104/1‑137 (MQ=255)
                                      tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcg                                                                                                      <  4:735033/140‑1 (MQ=255)
                                      tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGGCGGGGGGGGATGAGTAAAGGTGTCGTGGCATAACAtcg                                                                                                      >  5:939754/1‑140 (MQ=255)
                                      tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTGATGGCGCGGTCGGGGGCGGATGAGTTATGGTGTGGGGGGATAACAggg                                                                                                      >  6:579478/1‑138 (MQ=255)
                                         cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACCCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAATGGTGTCGTGGCATAACAgcgc                                                                                                     <  1:958716/138‑1 (MQ=255)
                                            cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCaa                                                                                                <  4:35215/140‑1 (MQ=255)
                                              ccGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCGG‑TTTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGACATAACAGCGCGGCAaca                                                                                              <  3:124747/139‑1 (MQ=255)
                                              aCGCTGAAACTCTTGCCGGGATATATGACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGAGG‑TTTTTGTACTGCTGGCGCGGTCGGTTGCGGTTGAGTAAAGGTGTCGTCGCATAACAGCGCGGCAaca                                                                                              <  3:1069608/140‑1 (MQ=255)
                                              aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCaa                                                                                                <  2:908595/138‑1 (MQ=255)
                                              aCGCTGAAACTCTTGACGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                              <  7:269747/140‑1 (MQ=255)
                                                  tGAAACTCTTGCCGCGATATATCACTCATAACACCCACCTGACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTGTAATGTTGGC‑CGGGGGGGTGACGGTGGCTAAACGTGTCGTGGCAGTACCGCGCGGCcacagcgc                                                                                          >  8:490278/1‑136 (MQ=255)
                                                    aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGa                                                                                          <  1:584889/138‑1 (MQ=255)
                                                     aaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCTGTCGGTTGCGGATGTGTAAATGTGTCGTGGGATAACAGCGAGGCAACACGGACGt                                                                                       <  5:347079/140‑1 (MQ=255)
                                                      aCTCTTGCCGGGATATATCACTCATAACACTCTCCTTCCATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTa                                                                                      <  5:801560/140‑1 (MQ=255)
                                                      aCTCTTGACGGGAGATCTCACTCTTAACACTCTCATTTCATTTTTTGTTTTTAGTGTAGAGGAGG‑TTTTTGTACTTCTGGGGAGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTa                                                                                      <  7:827311/140‑1 (MQ=255)
                                                        tctTGCCGGGATATATCACTCATAACACTATCCTTACATTATTTGTTTTTAGTGTAGAGGCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTCGCATAACAGCGCGGCAACACGGACGTa                                                                                      <  2:124880/138‑1 (MQ=255)
                                                          tttccGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCTGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACt                                                                                    <  2:608804/135‑1 (MQ=255)
                                                             ccGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                                               <  5:420331/140‑1 (MQ=255)
                                                             ccGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                                               <  6:877908/140‑1 (MQ=255)
                                                               gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCTG‑TTTTTGTACTTCTGGAGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  3:322859/140‑1 (MQ=255)
                                                               gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  6:1002463/140‑1 (MQ=255)
                                                                 gATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGAtct                                                                           <  5:178338/140‑1 (MQ=255)
                                                                      atCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTGTTTTAAATCGGGCGCGGTCGGGTGCGGCTGAGTAAATGGGTCGTGGCATAACAGGGCAGGAACACGGAAGTACTCGTTGATCTCttgc                                                                      >  5:614006/1‑138 (MQ=255)
                                                                          cTCATAACCCTCTCCTTACATTTTTTGTTGTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                                                  <  8:1005320/140‑1 (MQ=255)
                                                                          cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                                                  <  6:696935/140‑1 (MQ=255)
                                                                          cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTAATTATTGCGAGGTCGGTTGAGGATGAGTTAAAGTGTAGTGGGATAACAGCGCGGCAACAAGGACGTACTCGATTATATGTTCAAGCg                                                                  >  8:885983/1‑140 (MQ=255)
                                                                          cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTGCTTATGGGGCGGTGGGGTGGGGATGAGTAAAGGTGTCGTGGCATAACCGCGCGGCAACACGGCCGTTCTAGCTGATGTCTTAAAGCg                                                                  >  7:895093/1‑140 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTATTGGGCGGTCGGTTGGGGATGAGGAAATGTTTAGTGGGAGAACAACGCGGCAACACGGACGTACAAGCTGATAAATTCAAGCGa                                                                 >  3:927739/1‑140 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTGTGGGGCGGTCGGTTGCGGAAGAGGTAATGTGTGGGGGGATAACAGCGCGTCCACAAGGTCGGACTCGATGATCTCTTCAAGCgg                                                                 >  3:685532/1‑139 (MQ=255)
                                                                           acATAACACTCTACTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGAGGATGAGTAAAGGTGTCGTGGCATTACAGCGCGGCAACTCGGACGTACTCGATGATCTCTTCAAGCGa                                                                 <  3:269509/139‑1 (MQ=255)
                                                                                acacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAttt                                                            <  7:642703/140‑1 (MQ=255)
                                                                                   ctctCCTAACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCaa                                                         <  8:508683/140‑1 (MQ=255)
                                                                                    tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTGTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                                        <  5:1011956/140‑1 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTTCTTCTGGCGCGGTCGGGTGGGGATGAGTAAAGGTGTCGTGGCATAAAAGCGCGGCCAACCGGTCGTTGGCGATGTTCTAATCAAGCGACAGTTAAAGc                                                       >  6:138549/1‑140 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGTCGCGGTCGGTTGCGGATGAGTAAAGGTTGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCaa                                                         <  1:19409/138‑1 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  8:666664/140‑1 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  8:566890/140‑1 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGGCGGTTGAGGATTTGTTAATGTTTCGTGGCATAACAACGCGGGAACAATGCCGTACTAGATGATCTCTTCAAGAGACATTTCaatg                                                       >  6:5088/1‑138 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTAATTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTGGTGACATAACACCGCGGCAAAACGGAAGTACACAATTATCAATTAAAAAGACATTAAAAGc                                                       >  4:1014088/1‑140 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTTTTATTATGGAGCGGTCGGGTTAGGCTGATGTAAGGTGTGGTGGGATAAAAACGCGGCCACACGGACCTACTCGATGATCTCTTAAAGAGCCAATTTAAGc                                                       >  4:5020/1‑140 (MQ=255)
                                                                                     ctcCTTACATATTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTACTTATGGCGCGGGCGGGTTCGGCTGTGTAAATGTGTGGTGGCATAACAGCCCGGGCACCCGGACGAACTCGATGATCTCCTAAAGCGTCCATTCAAGc                                                       >  6:953738/1‑140 (MQ=255)
                                                                                     ctcATTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  3:218677/140‑1 (MQ=255)
                                                                                      tcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTACTTCAGGCGCGGTCCGGTGCGGATGATTTAACGTGTCGTGGCATAACAGCGCGGCAACACGGCCGTACGCGATGATCTCTTCAAGCGACATATCAATct                                                      >  7:739352/1‑140 (MQ=255)
                                                                                      tcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTATGGGGCGGGCGGGTGGGGATGAGTAAAGGTGTCGTGGCAAAAAAGCGCGGCACAACGGACGTACTCGATGATCTCTTCAAGCCACATTTCaac                                                        >  1:287132/1‑137 (MQ=255)
                                                                                       ccTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTAATTATTGCGCGGGAGGGTGCGGATTATGAAAGGTGTCTGGGCATAAAAGAGCGGCAACACGGACGTACTAGATTATCTCTTACATCGACATTTAAAGcta                                                     >  4:758847/1‑139 (MQ=255)
                                                                                           aCATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAATGTGTAGGGGCAATACAGCGCGGCTACCCGGACGGACACGATGATCTAATCAAGCGACAATTCAAGCTCTTcc                                                 >  4:928724/1‑140 (MQ=255)
                                                                                                ttttGTTTTTAGTGTAGAGTCGG‑TTTTTGTTCTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACCGCGCGGCAACACGGCCGGACACGTAGATCTCTTCAAGGGACCTTTCAAGCTGTTACGGAtg                                            >  6:430001/1‑139 (MQ=255)
                                                                                                  ttGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTATGGCGCGGTCGGGTGCGGATGAGGAAAGGGGTCGGGGCAGAACAGCGCGGCAACCCGGACGGACTCGCAGATCTGTTCAAGCGCCATTTGAAGCTGTTCCAGAtagt                                          >  8:723624/1‑137 (MQ=255)
                                                                                                       tttAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtct                                     <  4:631555/140‑1 (MQ=255)
                                                                                                           gtgtAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc                                   <  1:1073379/138‑1 (MQ=255)
                                                                                                           gggtAGTGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGGTGCGGTTGAGTAAAGGTGTCGTTGCATAACAGCGCGGCAACACGGCCGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                 <  7:691221/138‑1 (MQ=255)
                                                                                                             gtAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                 <  2:977116/138‑1 (MQ=255)
                                                                                                                     gg‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa                       <  8:138029/140‑1 (MQ=255)
                                                                                                                        ttttttTACTTAAGGCGCGGTCGGTTGCGGATGAGTAAATGTGTCGAGGCATAACAGCGCGGCAACACGGACGTACTGGATGATCTCTTCAAGCGACATTTCAAGCTGTTCCTGCTCTTCCTCTTAGTAGTAACCCAACt                     >  4:465812/1‑140 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAATGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  7:324913/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  3:940600/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  6:1016601/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  4:877988/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                    <  2:264318/138‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGGAAAGCTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  7:622818/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAACGGGGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCGCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  3:958841/140‑1 (MQ=255)
                                                                                                                             gTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  1:114462/138‑1 (MQ=255)
                                                                                                                             gTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                <  4:969539/140‑1 (MQ=255)
                                                                                                                                    tGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt           >  1:496663/1‑138 (MQ=255)
                                                                                                                                      gcgcGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGAAATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTActc       >  8:521319/1‑138 (MQ=255)
                                                                                                                                         cggtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa    <  8:833653/140‑1 (MQ=255)
                                                                                                                                         cgggcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa    <  5:125122/140‑1 (MQ=255)
                                                                                                                                           gtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                <  3:340634/126‑1 (MQ=255)
                                                                                                                                           gtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtc  >  5:258931/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                          
GCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATC  >  NZ_CP022686/1674105‑1674382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A