Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A27 F36 I0 R1 164 7919.5 7665810 98.4% 7543157 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,244 T→G 11.6% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2440TG11.6% 294.2 / 5.4 171T182P (ACC→CCC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (42/104);  new base G (17/8);  total (59/112)
Fisher's exact test for biased strand distribution p-value = 3.91e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

GCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATC  >  NZ_CP022686/1674105‑1674382
                                                                                                                                           |                                                                                                                                           
gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGcggt                                                                                                                                             <  6:662497/140‑1 (MQ=255)
gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGcggt                                                                                                                                             <  5:242126/140‑1 (MQ=255)
gcgcGGAGCTGCCGGGGTTGATTTGATATACCATGGCCTGACGGCGACGCTGAAACTCTTTCCGGGATATATCACTCATAACTCTCTCCTTACATTTTTTGTTTTTAGTGTATAGTCGGTTTTTGTACTTCTGGCGcggt                                                                                                                                             <  1:239768/140‑1 (MQ=255)
 cgcgGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAGATGGGGCGcggtg                                                                                                                                            >  6:676536/1‑139 (MQ=255)
         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCgg                                                                                                                                   <  2:860731/140‑1 (MQ=255)
         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCgg                                                                                                                                   <  4:897030/140‑1 (MQ=255)
         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCgg                                                                                                                                   <  6:783278/140‑1 (MQ=255)
         ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCgg                                                                                                                                   <  4:33349/139‑1 (MQ=255)
         ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCgg                                                                                                                                   <  6:49441/139‑1 (MQ=255)
           ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGAt                                                                                                                                 <  5:149441/140‑1 (MQ=255)
           ccGGGTTGCATTTGCTCTACCAGGGACTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGAt                                                                                                                                 <  4:163183/140‑1 (MQ=255)
            cGGGTTGCATTTGCGCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATg                                                                                                                                <  2:315267/140‑1 (MQ=255)
                 tGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCGGTTTTTGTACTTCTGGCGCGGG‑CGGTTGCGGATGAGTaa                                                                                                                           <  1:554016/140‑1 (MQ=255)
                  gCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTaaa                                                                                                                          <  4:911737/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  4:846127/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  8:452127/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  8:857549/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  2:700850/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  2:432229/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  6:416459/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCAATCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  8:305721/140‑1 (MQ=255)
                        gCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTCTTGTGGCGGGGT‑CGGGGGCGGATGACTAAAGGTGTc                                                                                                                    >  3:799087/1‑140 (MQ=255)
                        gCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTc                                                                                                                    <  5:528516/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACTCTGAAACTCTTGCCGGGATATATAACTCATAACACTGTCATTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                   <  4:164522/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                   <  6:910200/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                   <  5:676536/140‑1 (MQ=255)
                                   gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACGTGTTGCGCGGT‑CGGTTTCGGTTGAGTAAAGGGGTCGTTGAATAAcc                                                                                                         >  5:324006/1‑139 (MQ=255)
                                       gACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcgc                                                                                                     >  4:402248/1‑140 (MQ=255)
                                        aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTCTTATGGGGGGGG‑GGGGTTCGGATGAGTAAATGTGTCGTGGCGTAAAAgcgag                                                                                                    >  1:478663/1‑138 (MQ=255)
                                        aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACAATTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTTCATCTGGCGCGGG‑GGGGTGGGGATGAGTAAAGTTTGCGGGGCCTAACAGcgcg                                                                                                    >  3:22736/1‑140 (MQ=255)
                                        aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTACTTACAGTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGcgcg                                                                                                    <  8:465313/140‑1 (MQ=255)
                                         cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                                                   <  6:151210/140‑1 (MQ=255)
                                         cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                                                   <  6:861528/140‑1 (MQ=255)
                                            cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGG‑CGGGTGCGGTGGAGTAAAGGTGTGGTGGCATAACAGCGCGGcca                                                                                                >  8:793129/1‑138 (MQ=255)
                                              aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACGTATGGCGCGGG‑CGGTTGCGGCTGAGTAAATGTGTCGTGGCATAACAGCGCGGCAaca                                                                                              >  5:745573/1‑140 (MQ=255)
                                              aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCTTCTGGCGCGGG‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                              <  3:328474/140‑1 (MQ=255)
                                              aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                              <  6:744219/140‑1 (MQ=255)
                                              aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                              <  2:985803/140‑1 (MQ=255)
                                                gCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACg                                                                                            <  1:119730/140‑1 (MQ=255)
                                                  tGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTGGTGGCATAACAGAGCGGCAACACggc                                                                                          >  1:873181/1‑139 (MQ=255)
                                                    aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACg                                                                                        <  4:638047/140‑1 (MQ=255)
                                                    aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACg                                                                                        <  8:558704/140‑1 (MQ=255)
                                                             ccGTGATATATCACTCATAACACTCTCATTACATTTTTTGATTTTAGTGTAGAGTCGGATTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATATCAGCGCGGCAACACGGACGTACTCgatg                                                                               <  6:446262/140‑1 (MQ=255)
                                                             ccGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                                               <  8:813536/140‑1 (MQ=255)
                                                               gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  1:211894/140‑1 (MQ=255)
                                                               gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  5:789820/140‑1 (MQ=255)
                                                                     aatCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTc                                                                       <  4:149433/139‑1 (MQ=255)
                                                                        aaCTAATCACACTCTCCTTTCATTTTTTGTTTTTTGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGTTTGCGGATGAGTAAAGGGTTCGTGGCATATCAGCGCGGCTACACTGAGGTACTCGATGATCTCTTCaat                                                                    <  6:902722/139‑2 (MQ=255)
                                                                          cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTCTTAGGGCGCGGTGCGGTT‑CGGATGAGGAAAGGTGTAGTTGCATAACAGCGCGGGCACACGGCCGTTCTCGCTGATCTATTCAAGCg                                                                  >  7:784986/1‑140 (MQ=255)
                                                                          cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTATGGGGCGGT‑AGGTTGCGGCTGAGTTAACGTGTCGTGGCATTAACGCGCGGCCACACGGCCCTGCTCGCGGATCTCTTGAAGCg                                                                  >  4:483331/1‑140 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGTCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                 <  7:179569/140‑1 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                 <  2:242607/140‑1 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCGGGCGCGGT‑CGGGTGCGGGTGAGTAAATGGGTCGTGGCATAACAGCGCCGCAACACCGCCCTACTAGATGATAGCTTCAAGCGa                                                                 >  3:887343/1‑140 (MQ=255)
                                                                           tCATAACACTCTCCTTACAATTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                 <  6:216575/140‑1 (MQ=255)
                                                                           tCATAACACTCTCCATACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                 <  2:621597/140‑1 (MQ=255)
                                                                           acATAACACTCTCCTTACATTTTTTGTTTTTACTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACATCGCGGCAACACGGACGTACTCGATTATCTCTTCAAGCGa                                                                 <  6:95815/139‑1 (MQ=255)
                                                                             aTAACACTCTCCTTACATTTTTTGGTTTTAGTGTAGAGTCGGGTTTTGTTCTTATGGGGCGGG‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGCCGTACACGATGATCACTTCCAGAGACa                                                               >  8:222304/1‑140 (MQ=255)
                                                                               aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTCAGGCGCGGG‑CGGTTGGGGAGGAGTAAAGGGGTCGTGGCAAAACAGCGCGGGCACACTGTACTACTAGATGATCTCTTAAACCCACAtt                                                             >  4:692502/1‑140 (MQ=255)
                                                                               aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTATTATGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTTTGGTGGCATAACAGGGCGGCAACACGGACGTAATCGATGATCTCGTCAAGCGACAtt                                                             >  3:790857/1‑140 (MQ=255)
                                                                                acacTCTCCTTTCATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAttt                                                            <  4:658040/140‑1 (MQ=255)
                                                                                acacTCTCCTTACATTTTTTGTTTTTCGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAttt                                                            <  7:231881/140‑1 (MQ=255)
                                                                                acacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAttt                                                            <  1:919094/140‑1 (MQ=255)
                                                                                   ctctCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTGGGGGCATAACAGCGCGGCAACACGGGCGGACTCGATGATGTCTTCAAGCGACATTTCaa                                                         >  6:937880/1‑140 (MQ=255)
                                                                                    tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTGCGTCTGGGGCGGG‑AGGGTTCGGATGCGGAAAGGGGGCCTGGCAGATCAGCGCGGCCACACGGGAGTAATCGCTTATAGCTTCAAGCGCCATTTCatg                                                        >  4:704784/1‑138 (MQ=255)
                                                                                    tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                                        <  8:376827/140‑1 (MQ=255)
                                                                                    tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGG‑CGGTTGCGGATGAGTAAAGGTGGAGTTGGATAACAGCGCGGCAACACGGACGTACTAGATGATATCATCAAGCGACATTTCaac                                                        >  7:285429/1‑139 (MQ=255)
                                                                                    tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTATGGGGCGGT‑GGGTTTGGGATGGGTTTAGGTGTCGGGGGATAAAAGCGCGGCCACACGGACGTGCTCGATGATCGATTAAAGCGGCATTTGAAg                                                        >  2:836203/1‑140 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTATTGCGCGGT‑AGGTTGCGGAAGAGTAAAGGTGTGGTGGGATAAAAGCGCGGCAACCCCGCCGTACTCGATTATCTCTTCAAGCGACATTTCAAGc                                                       >  8:246711/1‑140 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGGTGATGAGTAAAGGTGTCGTGGCATAACAGCGAGGCAACACGGACCTACTAGATGATATCATAAAGCGACAATTAAAGc                                                       >  8:757137/1‑140 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  1:690286/140‑1 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACATCTGGCGCGGG‑CGGTTGCGGATGAGAAAAGCTGACGTGGCATAACAGCCCGGCAACCCGGAAAAACACGATGATCACATCAAGCAACAATTCAAGc                                                       >  8:689854/1‑140 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGGATAACAGCGCGGCAACCCCGACGTACTCGATGATCTCATCAAACGCCATTTCAAGc                                                       >  4:950764/1‑140 (MQ=255)
                                                                                     ctcCTTACAATTTTTGTGTTTAGTGGAGAGTCGGTGTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  7:603838/140‑1 (MQ=255)
                                                                                       ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGGGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAACCGACATTTCAAGctg                                                     >  3:573528/1‑139 (MQ=255)
                                                                                       ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGGTGCGGATGAGTAAAGGAGGCGTGGCCAAACCGCGCCGCACCACCGCCGGACGCGAGGATATCTTCAAGCGACAAATAAAGctc                                                     >  8:422008/1‑140 (MQ=255)
                                                                                       ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTCTTCTTGCCCGGG‑AGGGTGCGGATGAGTAAAACGTTCGGGGAGTAAAACCGCGGCAACACGGCCCTACACGATGATCACTTCAAGCCACCATTCAAGgtc                                                     >  6:693017/1‑140 (MQ=255)
                                                                                          tACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTc                                                  <  3:402248/140‑1 (MQ=255)
                                                                                           aCATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                                                 <  8:40106/140‑1 (MQ=255)
                                                                                           aCATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                                                 <  5:462629/140‑1 (MQ=255)
                                                                                                ttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGG‑CGGTTGCGGATGTGTAAAGGTGTCGTGGCCTAACCGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAACTCTTGCTGAta                                            >  4:839975/1‑139 (MQ=255)
                                                                                                ttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTAAGGGGCGGG‑CGGGTTGGGAGCTTTAAAAGTGTCGGGGGCTAAAACCCCCGCAACACGGCCGGAAACGATGAACGCTTAAAGCGAAAAATAAACCTCTTccgtatg                                            >  2:933221/1‑135 (MQ=255)
                                                                                                       tttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtct                                     <  4:203968/140‑1 (MQ=255)
                                                                                                        ttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTTGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt                                    <  6:570467/140‑1 (MQ=255)
                                                                                                           gtttAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                 <  5:795869/137‑1 (MQ=255)
                                                                                                           gtgtAGAGTCGGTTTTTGTACGTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                 <  7:547888/140‑1 (MQ=255)
                                                                                                             gtAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGGAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTtcgtcg                               <  1:26638/140‑1 (MQ=255)
                                                                                                               agagGCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTa                             <  2:290043/140‑1 (MQ=255)
                                                                                                                          ttGTACTTGTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  1:903866/140‑1 (MQ=255)
                                                                                                                          ttGTACTTCTGGCGCGTT‑AGGTTGCGGATTAGTAAAGGTGTAGTGGCATAACAGCGCGGCAACACGGACGTACTAGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  1:172189/140‑1 (MQ=255)
                                                                                                                          ttGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  6:116439/140‑1 (MQ=255)
                                                                                                                          ttGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  2:61486/140‑1 (MQ=255)
                                                                                                                          ttGTACTTCTGGCGCGGG‑CGGGTGCGGATGAGTAAAGGTGTCGTGGCATACCAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  5:552339/140‑1 (MQ=255)
                                                                                                                            gTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATTATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                <  3:41289/140‑1 (MQ=255)
                                                                                                                            gTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                <  4:229507/140‑1 (MQ=255)
                                                                                                                                 tCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCACCACGGCCGTACTCGATGAGCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTCCGCGAAGtt           >  8:625558/1‑140 (MQ=255)
                                                                                                                                 tCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt           >  1:4941/1‑140 (MQ=255)
                                                                                                                                    ggCGCGGT‑CGGTTGCGGATGAGTAAAGTTGTCGTGGCTTAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTTTTCCTGATATTCGTCTTCGTCGTAACCCAACTGCGGGATGTTACg        <  4:921096/140‑1 (MQ=255)
                                                                                                                                     gcgcGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGc       >  2:454595/1‑140 (MQ=255)
                                                                                                                                     gcgcGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGc       >  3:616217/1‑140 (MQ=255)
                                                                                                                                          gt‑cggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                                                  <  5:49140/76‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGT‑CGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATC  >  NZ_CP022686/1674105‑1674382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A