Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A29 F2 I0 R1 207 10827.9 9263290 98.4% 9115077 135.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,244 T→G 14.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2440TG14.4% 300.2 / 7.9 195T182P (ACC→CCC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (53/108);  new base G (23/11);  total (76/119)
Fisher's exact test for biased strand distribution p-value = 3.64e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

GCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATC  >  NZ_CP022686/1674105‑1674382
                                                                                                                                            |                                                                                                                                            
      cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTg                                                                                                                                        <  1:1010950/140‑1 (MQ=255)
        cTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTAATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGGTTTGTACTTATGGGGCTGTCGGGTg                                                                                                                                        >  6:258011/1‑138 (MQ=255)
         tGCCGGTTTTCATTTGCTCTTCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTACgg                                                                                                                                     <  2:1141045/140‑1 (MQ=255)
         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                     <  2:895779/140‑1 (MQ=255)
         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGGCGGTTGc                                                                                                                                       <  5:1062592/138‑1 (MQ=255)
         tGCCGGGTTGCATTTGCGCTACCAGTGCCTGACGGCGACGCTTAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑TGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                     <  2:504425/140‑1 (MQ=255)
         ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCCTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                                                       <  6:276196/137‑1 (MQ=255)
         ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                     <  3:651201/139‑1 (MQ=255)
         ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                     <  1:84053/139‑1 (MQ=255)
          gCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCg                                                                                                                                      <  8:339390/138‑1 (MQ=255)
           ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                     <  7:1033010/138‑1 (MQ=255)
           ccGGGGTGCATTTGCTCTACAAGGGCCTGACGTCGACGCTGAACCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑TGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                     <  6:780637/138‑1 (MQ=255)
                ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACTTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTGT‑TTTGTGGGGCGGTCGGGTgggggttaggt                                                                                                                              >  4:207490/1‑133 (MQ=255)
                ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTTCTTGGGGCGCGGGCGGTTGCGGTTG‑AGTa                                                                                                                             >  3:344498/1‑140 (MQ=255)
                   cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTTATTCTGGCGCGGTCGGTTGCGGATG‑AGTaaat                                                                                                                          >  1:409463/1‑139 (MQ=255)
                       tgagctACCAGGGCCTGTCTGCGAGGCTGATAATCTATTGCGGCTATATCACTCTTAACACTCTCCTTTAATATTTTGTTTTTAGTGTTGAGTC‑GGGGTTTGTACTTCTGGCGCGGTCGGGTGCGGATT‑AGATAAGgt                                                                                                                        <  8:400762/134‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACTCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgt                                                                                                                        <  5:251499/138‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGTAACTCTTGCCGTGATATATCACTCATAACACTCTACTTTCATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                                      <  1:871155/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCGTAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGGTGCGGATG‑AGTAAAGgtgt                                                                                                                      <  1:722658/140‑1 (MQ=255)
                         ctctTCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                                      <  6:630135/138‑1 (MQ=255)
                         ctctAGCAGGGCCTTACGGCGACGCTGAAACGCTTGCCGGGATATATCACGCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCg                                                                                                                    <  2:1078736/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTGCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCg                                                                                                                    <  2:83705/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                                      <  6:97923/138‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                                      <  6:538156/138‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCg                                                                                                                    <  3:146467/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCg                                                                                                                    <  4:738708/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCg                                                                                                                    <  1:43416/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGAGATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                                      <  6:1008558/138‑1 (MQ=255)
                               cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTTCTTCTGGCGCGGTGGGGTGGGGTGG‑AGTAAATGTGtggtggg                                                                                                                >  7:439123/1‑137 (MQ=255)
                                  ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTAATTATTGGGCGGGAGGTTGCGGATG‑AGTAAAGTTGTGGTTGCA‑Taaa                                                                                                          >  1:782290/1‑139 (MQ=255)
                                      tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTTTTCAGGTGGCGCGGTCGGGTGGGGTGG‑AGTAAAGGTGTCGGGGAA‑TAACAggc                                                                                                      >  2:191112/1‑138 (MQ=255)
                                        aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTTCTTATGGCGCGGGCGGTTGCGGGTG‑AGTTAATGTGTAGTGGGA‑TAACAGcgcg                                                                                                    >  1:584406/1‑140 (MQ=255)
                                        aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTTATTGTGGCGCGGGGGGGTGGGGATG‑TGTAAAGGTGTGGTGGGA‑TAACAGcgcg                                                                                                    >  3:555313/1‑140 (MQ=255)
                                            cGACGCTGAATCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTCGCA‑TAACAGCGCGGCaa                                                                                                <  3:332813/140‑1 (MQ=255)
                                            cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCaa                                                                                                <  3:424162/140‑1 (MQ=255)
                                              aatcTGAAACTCTTGCCGGGATATAGCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTCGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCaa                                                                                                <  7:30103/135‑1 (MQ=255)
                                              aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTAATTCAGGCGCGGTGGGTTGGGGATG‑TGTAAAGGTGTGCTGGCA‑TAACAACGCGGGAacc                                                                                              >  1:1187520/1‑139 (MQ=255)
                                              aCGCTGAAACTCTTGACGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCTGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCaa                                                                                                <  7:997629/138‑1 (MQ=255)
                                                 cTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAacac                                                                                             <  7:413108/138‑1 (MQ=255)
                                                   gAAACTCTTGCAGGGATATATCACTCATAACACTCTCCTTTCATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGTTG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGAc                                                                                         <  1:1070836/140‑1 (MQ=255)
                                                     aaCTCTTTCCGGTATATATCACTCCTCACACTCGCCTTACATTTTTTGTTGTTAGTGTAGAGTC‑TGATTTTGAACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAATGGGGTCGTGGCA‑TAACAGCGCGGCAACACGGACGt                                                                                       <  4:850316/140‑1 (MQ=255)
                                                     aaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTATAGTC‑GGTTTTTGTACTTCTGGCGAGGTCGGTTGCGGATG‑AGTAAAGGTGTCTTGGCA‑TAACAGCGCGGCAACACGGAc                                                                                         <  6:333091/138‑1 (MQ=255)
                                                       ctctTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGGCGGTTGCGGATG‑AGTAAAGGTGTGGTGGCA‑TAACAGCGCGGCAACACGGCCGt                                                                                       >  5:580317/1‑138 (MQ=255)
                                                           tGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTTTACTTCTGGCGCGGGGGGTTGGGGTTGTAGT‑AAGGTGTAGTGGCG‑TAACAGCGCGGAAACACGGCCGGAcgcgc                                                                                 >  3:246163/1‑139 (MQ=255)
                                                               gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  1:544418/140‑1 (MQ=255)
                                                               gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGGCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCgatg                                                                               <  5:956791/138‑1 (MQ=255)
                                                                        cACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTTTTGCGCGGTCGGGTTCGGATT‑AGGAAAGTTGTGGTGTCA‑TAACAGAGCCTCCTCACGGACGTACTAGATTATATCttaa                                                                      >  6:445257/1‑136 (MQ=255)
                                                                          cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAg                                                                    <  7:352367/138‑1 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCgc                                                                 >  4:1022957/1‑139 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTTATTCTGGCGCGGTGGGGTTGGGATG‑AGTAAAGGTGGGGGGGGG‑TAACAGCGCGGCAAAACGGACGTACTCGATGATAACGTCAATCGa                                                                 >  4:732516/1‑140 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGc                                                                   <  5:676274/138‑1 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGAGGCAACACGGACGTACTCGATGATCTCTTCAAGc                                                                   <  6:924458/138‑1 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCATGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCCACACGGACGTACTCGATGATCACTTAAAGCgg                                                                 >  2:752420/1‑139 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGc                                                                   <  8:1058104/138‑1 (MQ=255)
                                                                            cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGAc                                                                <  2:539592/140‑1 (MQ=255)
                                                                            cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACATATGGCGAGGTCGGGTGCGGATG‑AGGAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTAATAGATGATCTATTAAAGCg                                                                  >  8:211643/1‑138 (MQ=255)
                                                                             aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTATGGCGCGGTCGGTTGCGGATG‑AGTAAATGTGGGGTGGGC‑TAACAGCGCGGCAACACGGGCGTACTCGATGATCGCGTCAAGCGACa                                                               >  4:933244/1‑140 (MQ=255)
                                                                               aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTACTTATGGGGCGGTGGGTTGCGGATG‑ATTAAATGTGTGCTGGCA‑TAACACCGCGGCCACCCGGCCGGACGAGATGATCTCTTCAAGCCACAtt                                                             >  4:336946/1‑140 (MQ=255)
                                                                                acacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAttt                                                            <  2:583968/140‑1 (MQ=255)
                                                                                 cacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAtt                                                             >  7:169588/1‑138 (MQ=255)
                                                                                 cacTCTCCTTACATTTTTTGTTTTTAGTGGAGAGGC‑GGGTTTTGGACTTCAGGCGCGGTCGGGTGCGGTTG‑AGGTAAAGTGTCGTGGCA‑TAAAAGCCCCGCCACCCGGCCGGACGCGATGTTCTCTTCAACCGCCAtt                                                             >  6:712102/1‑138 (MQ=255)
                                                                                    tctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTAATTATGGCGCGGTCGGTTGCGGATG‑AGTAAAAGTGTCGTGGCA‑TAAAAGCGCGGCAACACTGACGTACTCGAAGATCTCTTAAAGCGACATTTaaac                                                        >  4:684655/1‑139 (MQ=255)
                                                                                    tctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGGGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCa                                                          <  5:699682/138‑1 (MQ=255)
                                                                                    tctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGATTTTGTACTTCTGGCTCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                                        <  3:76681/140‑1 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTACATATGGCGCGGTGGGTTTGGGTTG‑AGTAAAGGTTTCGGGGGA‑TAAAAACGCGGCTACAAGGACATACTCGATGATCGCTTGAATCGACATATAAAGc                                                       >  4:38497/1‑140 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  1:958922/140‑1 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  3:833267/140‑1 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGAC‑CGTTGTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  2:799726/140‑1 (MQ=255)
                                                                                       ccTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAATGTGTCGTGGCA‑TAACAGCGCGGCAACACGGCCGTAAGAGATTATCTATTCAAGCGACATTTAAAGc                                                       >  6:676274/1‑138 (MQ=255)
                                                                                       ccTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACATATGGGGCGGGCGGTTGCGGATG‑AGTAAAAGTTTGGGGGGA‑TAACAGCGCGGCAACACGGACGGACTCGATGATCAATTAAACCGCCAATTAAAGc                                                       >  5:499825/1‑138 (MQ=255)
                                                                                       ccTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTACTTCTGGCGCGGGCGGTTTCGGATG‑AGTAAAGGTGTAGTGGCA‑TAACAGCGCGGCAACACGGACGGACTCGATGATATCTTCAAGCGCCATTTAAAGc                                                       >  8:526699/1‑138 (MQ=255)
                                                                                       ccTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTAATTCTGGCGCGGTGGGTTGCGGATG‑AGTTAAGGTGTCGTGGCA‑TAACAGCGCGGCACCACGGACGTACTCGATGCTATCGTCAAGCGACATTTCAAGc                                                       >  7:533204/1‑138 (MQ=255)
                                                                                         ttACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTTTAATTATGGGGCGGGGGGTTGGGGAGT‑AGGTAAAGTGGCGTGGGATTTACA‑CGGGGCGCCACGGCCGGACAAGATGATCTCTTCAAGGGTCCTATCAAtctc                                                     >  6:1090952/1‑138 (MQ=255)
                                                                                           aCATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCtt                                                   <  8:169588/138‑1 (MQ=255)
                                                                                           aCATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTTCTTGAGGCGCGGGGGGGTGCGTCGG‑AGTAAAGGGGTAGTGGCA‑GAACAGCGCGGCCACACGGCCCATCGGCATGATATCTTCAAGAGCCAATTAAAGcgattac                                                 >  4:1133498/1‑134 (MQ=255)
                                                                                                ttttGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtc                                            <  4:551816/140‑1 (MQ=255)
                                                                                                ttttGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGGCGGTGTAGGCTG‑AAGAAAGGTGTCGTGGGA‑TTACACCGCCGCCACGCCGCCGGACTCCAGGATCACTTCAAACATCCTTTAAAGCTCTTGCTGa                                              >  6:955593/1‑138 (MQ=255)
                                                                                                  ttGTTTTTAGTGTAGAGTC‑GGGTTTTGTACATATGGGGCGGTCGGTTGCGGATG‑AGGTAAGGTGTCGTGGCA‑TAACCGCGCGGCCACACGGACGTACACGATGATCTCATACAGCGACATTTAAAGCTATTCCGGaaa                                            >  8:637974/1‑136 (MQ=255)
                                                                                                    gTTTTTAGTGTAGAGTC‑GGGTTTTGTACTTATGGGGCGGTCGGTTGGGGATG‑AGGAAAGGTGTGGTGGCA‑TAACAGCGCGGCCACACGGGCGTACTCGATGATCTATTCAAGCGACATTTAAAGCTCTTGCTGAtggt                                          >  6:131697/1‑135 (MQ=255)
                                                                                                       tttAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtct                                     <  1:274724/140‑1 (MQ=255)
                                                                                                        ttAGTTGAGAGTC‑GGTGTTTGTACTTCTGGCGCTGTCGGTTGCGGATG‑AGTAAAGGTGGCGTGTCA‑TAACAGCGCGGCAACACGGACTTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtc                                      <  7:119057/138‑1 (MQ=255)
                                                                                                           gtgtAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                 <  2:861533/140‑1 (MQ=255)
                                                                                                              tAGAGTC‑GGATTTTGTACTTGTGGCGCGGGCGGGTGCGTTTG‑AGTCAAGGTGTCGTGGCA‑TAACAGCGATGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTcgtcgt                              <  2:1168716/140‑1 (MQ=255)
                                                                                                                 agTC‑GGGGTTTGTTCTTGTGGGGCGGTAGGTTGGGGATG‑AGGTAAGGGGTCGTGGGA‑TAACAGCGCGGCAACACGGACGGACTAGATGATCTCTTAAAGCGACATTTAAAGCTATTACGGATATACCTCTTAGTAGTAAc                           >  4:597762/1‑140 (MQ=255)
                                                                                                                      ggTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCACGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa                       <  2:105605/140‑1 (MQ=255)
                                                                                                                       gTTTTTTTACTTATGGCGCGGGCGGGTGCGGATT‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCCACACGGACGTACTCGATGATATCTTCAAGCGACATTTAAAGCTCTTACTGCTCTTAGTCTTCGTCGTAAACCAAc                      >  2:436243/1‑140 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                    <  6:889803/138‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGGCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                    <  7:516792/138‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGGCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  4:431916/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGGCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                    <  5:8675/138‑1 (MQ=255)
                                                                                                                           ttGTACTTCGGGCGCGGTCTGATGAGGAAG‑TGTAAAGGATTCGTGGCA‑TAACAGCGCGGAAACACGGAGGTACTCGATGATCGCTTCACGCGACATTTCAAGCTCTTCCTGTTCTTCTTCTTCGTCGTAACCCAACTg                    <  5:634243/138‑1 (MQ=255)
                                                                                                                             gTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  5:336655/138‑1 (MQ=255)
                                                                                                                             gTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                <  3:22819/140‑1 (MQ=255)
                                                                                                                             gTACTTCTGGCGCGGGCGGGTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  5:829741/138‑1 (MQ=255)
                                                                                                                             gTACATCTGGCGCGGGCGGTTGCGGATG‑AGTAAAGGTGTCGAGGCA‑TAACAGCGCTGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  7:709868/138‑1 (MQ=255)
                                                                                                                                  tCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGTCT‑TACCAGCGCGGCCACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGCTCTTCGTCTTAGTCGTAACCCAACAGCGAGATg             >  8:368522/1‑138 (MQ=255)
                                                                                                                                    tGGCGCGGTCGTGAGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGCTCTCTTCACGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTACACCCACTGCGCCCTGtt           >  5:155329/1‑138 (MQ=255)
                                                                                                                                    tGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAATCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt           >  5:806210/1‑138 (MQ=255)
                                                                                                                                           gtcggtTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTATCCCAACTGCGCGATGTTACGCAGAtc  >  4:557333/1‑140 (MQ=255)
                                                                                                                                           gtcggtTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGCtc  >  3:312282/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATC  >  NZ_CP022686/1674105‑1674382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A