Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A27 F2 I0 R1 73 6005.1 5484070 98.3% 5390840 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,244 T→G 11.7% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2440TG11.7% 194.2 / 3.5 115T182P (ACC→CCC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (28/71);  new base G (12/4);  total (40/75)
Fisher's exact test for biased strand distribution p-value = 5.12e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATC  >  NZ_CP022686/1674111‑1674382
                                                                                                                                     |                                                                                                                                           
cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTGTGGCGCGGGCGGTTg                                                                                                                                       >  2:587772/1‑140 (MQ=255)
cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTg                                                                                                                                       <  4:564403/140‑1 (MQ=255)
  cTGCCGGGTTGCATTTGCGCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGGTGCg                                                                                                                                     <  4:6075/140‑1 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                    <  4:359454/140‑1 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                    <  2:375710/140‑1 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                    <  3:556983/140‑1 (MQ=255)
   ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                    <  2:655680/139‑1 (MQ=255)
     ccGGGTTGCATTTGCTCTTCCTGGGCCTGACTGCGACGCTGATACTCTTTCCGGGATTTATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGTTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                                                  <  2:39436/140‑1 (MQ=255)
          ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑Ta                                                                                                                            <  4:259815/140‑1 (MQ=255)
            gCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGAGATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTATTCTGGCGCGGTAGGGTTGGGATGAG‑tt                                                                                                                            >  7:656044/1‑137 (MQ=255)
             cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAg                                                                                                                         <  2:692234/140‑1 (MQ=255)
                 tGCTCTAGCAGGGCCTGTCGGGGACGCTGAAACTATTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TTAAGgt                                                                                                                       <  8:608025/138‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGTAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                     <  1:505124/140‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                     <  5:281181/140‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATAACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                     <  3:450655/140‑1 (MQ=255)
                  gCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTATGGGGCGGTGGGTTTCGGATGAG‑TAAATGTGTc                                                                                                                    >  5:326619/1‑140 (MQ=255)
                   ctctACCAGGGCCTGTCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACTTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCg                                                                                                                   <  1:201855/140‑1 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                     <  7:88485/138‑1 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                     <  7:327745/138‑1 (MQ=255)
                   cgctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                     <  7:136644/136‑1 (MQ=255)
                       aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATTTATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTAGGGTTTTGTAAGTATGTGGCGGTTGTTTGAGGCTGAG‑TTAATGTGTCGTggg                                                                                                               >  1:211605/1‑139 (MQ=255)
                         cAGGGCCTGACGGCGACGCTTAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGc                                                                                                               <  7:377834/138‑1 (MQ=255)
                            ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTATGGCGCGGTCGGGTGCGGATGAG‑TAAAGGTGTAGTGGCATatc                                                                                                          >  2:681950/1‑138 (MQ=255)
                            ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCGTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGGCGGGTGCGGATGGG‑TAAAGGTGGCGTGGCGTaaa                                                                                                          >  5:629521/1‑139 (MQ=255)
                             gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATACCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTGACTTCAGGCGCGGTCGGTTGCGGATGTG‑GAAAGGTGTCGTTGCGTAACa                                                                                                         >  1:407846/1‑140 (MQ=255)
                             gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCGCCTTACATTTTTTGTTTTTAGTGTAGAGGCGGGTTTTGTGCTTCGGGCGCGGTAGGTTGGGGGTGAG‑GAAAGGGGTCGGGGCCTAAcc                                                                                                         >  2:146001/1‑139 (MQ=255)
                              ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTGTTGTAAGTCTGGCGCGGGCGGTGGGGGATGAG‑TTAAGGTGTCGTGGCATAACAg                                                                                                        >  4:18417/1‑140 (MQ=255)
                                 gACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAgcgc                                                                                                     <  1:311750/140‑1 (MQ=255)
                                   cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCATTACATTTTTTGTTTTTAGTGTAGAGGCGGTTTTTGTACTTCTTGCGCGGTCGGTTGCGTATGAG‑TAAAGGTGTCGTGGCATAACAGCGCgg                                                                                                   <  5:478373/140‑1 (MQ=255)
                                      cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTCTGGCGGGGGCGGTGGCGGCTGAG‑TAAAGGTGTGGGGGGGTAAAAGCGCGGccc                                                                                                >  4:686228/1‑138 (MQ=255)
                                        aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                              <  4:254153/140‑1 (MQ=255)
                                          gCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGGCGTGGCATAACAGCGCGGCAACACg                                                                                            <  4:45002/140‑1 (MQ=255)
                                          gCTGAAACTCTTGCCGGGATATATCACTCATAACACGCTCCATACATTTTTTGTTTTTAGTGTAGAGGCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACg                                                                                            <  6:21341/140‑1 (MQ=255)
                                           cTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACgg                                                                                           <  3:289888/140‑1 (MQ=255)
                                              aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGGGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACg                                                                                        <  2:158029/140‑1 (MQ=255)
                                                   ctTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTT‑TACTTTTGGCGCGGGCGGTGGCGGCTGAGTTAAAGGTG‑AGTGGCATAACAGCGCCGCAACAAGGACGGACACg                                                                                  >  2:76960/1‑140 (MQ=255)
                                                    ttGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTAAATCTGGCGCGGGCGGGTGCGGATGAG‑TAAAGGGGTGGTGGGATAACAGCGCGGCCACACCGACGTACTCg                                                                                  >  3:432825/1‑140 (MQ=255)
                                                       ccGGGATATATCACTCATAACACTCTCCCTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCga                                                                                 <  7:470221/138‑1 (MQ=255)
                                                         ttgATATATCAAGAATAAAACTCTCATTACATTTTTTGCTTTTCTTGTAGAGTCTGTTTTTGTACTTCTGGAGCGTTCGGTTGCGGATGAT‑TAAAGGTTTCTTGGCATAACAGCGCGGAAACACGGACGTACTCgatgat                                                                             <  2:156749/138‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTTCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgattat                                                                             <  4:565965/140‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  1:587772/140‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  3:479068/140‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  2:288677/140‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  1:188479/140‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  6:209411/140‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                                               <  8:198060/138‑1 (MQ=255)
                                                         gggAGATATCACTCATAACACTCACCTTACATTTTTTGTGTTTAGTGTAGAGGCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                                               <  8:476487/138‑1 (MQ=255)
                                                              atatCACTCATAACCCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGTCGGGTGCGGGTGAG‑TAAAGGTGTAGTGGGCTAACAGCGCGGGCACCAGGACGGACTCGATGAtcta                                                                          >  8:422749/1‑137 (MQ=255)
                                                              atatCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGATTCGGTTTTTGTACTTCTGGCGCGGGAGGGTGCGGATGAG‑TAAAGGTGTAGTGGCATAACAGAGCGGCAACACGGACGTACTCGATGATATCtt                                                                        >  1:156408/1‑140 (MQ=255)
                                                                    cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAg                                                                    <  7:321874/138‑1 (MQ=255)
                                                                    cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAG‑TAAAGGTGTCGTGGCATAAAAGCGCGGCAAAACGGACGTACTCGATGATCTCTTCAAGCg                                                                  >  2:631934/1‑140 (MQ=255)
                                                                     tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                 <  4:131342/140‑1 (MQ=255)
                                                                      cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                                               <  7:85419/125‑1 (MQ=255)
                                                                      cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGAc                                                                <  3:307792/140‑1 (MQ=255)
                                                                      cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACGTCTGGCGGGGGCGGTTGCGGATGAG‑TAAAGGTGTGGTGGGATAACAGCGCGGCCACCCGGCCGTACTCGATGCTAGCGTCAAGCgcc                                                                >  4:391689/1‑138 (MQ=255)
                                                                       aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGGGCGGGCGGTTGCGGATGAG‑TAAAGGTTTCGTGGGATAACAGCGCGGGCACACGGACGTACTCGAGTATCTCTTCAAGCGACa                                                               >  4:366981/1‑140 (MQ=255)
                                                                       aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TTAAGGTGTAGTGGCATAAAAGCGCGGCAACACCGCCGGACTGGATGTTCTCATCAAGCGCCa                                                               >  3:601316/1‑140 (MQ=255)
                                                                       aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTGTTTGTAATTCTGGGGCGGTCGGTTGCGGCTGAG‑TAAAGGGGTCGTGGGCTAACAGCGCGGCCCCCCGGCCGTACGCGATGATCTCTTCACGCGCCa                                                               >  4:613935/1‑140 (MQ=255)
                                                                         aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGCGTCGGTTTTTTTAATTCAGGCGCGGTCGGGTGCCGAAGTG‑TTAAGTTGTCGTGGCCTAACAGCGCGGCAAAACCGACGGACTCGCTGAACTCATCAAGctaaa                                                               >  8:84063/1‑134 (MQ=255)
                                                                         aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTATTCTTGCGCGGTGGGGTTCGGTTTAG‑TAAAGGTGTCGTGGCATAACAACGCGGCAACACGGACGTTCTAGATGGTATCTTGAAGCGACAtt                                                             >  5:546508/1‑140 (MQ=255)
                                                                           cacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGTCGGTTGGGGATGAG‑TAAAGGTGTGGTGGCATAACAGCGCGGCAACACGGCCGTACTCGATGATCTCTTAAACCGACAtt                                                             >  8:499003/1‑138 (MQ=255)
                                                                              tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                                        <  3:75827/140‑1 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTATGTCGCGGTCGGTTGAGGATGAG‑TAAATGTGTCCTGGCAAAACACCGCGGACACACCGACGGACTCGATGATATCTTCAAGCGCCATTTAAAGc                                                       >  6:95248/1‑140 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCaa                                                         <  7:569912/138‑1 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTTCTTCTGGGGCGGTCGGGTGCGGTTGAG‑TTAAAGTGTCGTGGGATAACAGCGCGGCAAAACCGAAGTACACGATGATCTCATCAAGCGACATTTCAAGc                                                       >  3:36936/1‑140 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTCTTATGGCGCGGGCGGGTGCGGATGAG‑TAAAGGTGTGGTGGCATAACAGCCCGGGAACACCGACCTACTCGATGCTCTCTTAAAGCCACATTTAAAGc                                                       >  2:217153/1‑140 (MQ=255)
                                                                                tcCTTACATTTTTTGTTTTTTGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGct                                                      <  1:622584/140‑1 (MQ=255)
                                                                                 ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTATGGCGCGGGGGGTTGCGGATGAG‑TAAAGGTGTAGTGGCAGAACACCTCGGCACAAAGGACGTACTTGATGCTCTCTTCAAGCGACATTTGAAGc                                                       >  7:643514/1‑138 (MQ=255)
                                                                                 ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCTTCTGGCCCCGTCCGTTGGGGAGGAG‑TAAAGGTTTAGGGGCCTAACAGAGCCGCAACACGCACACACTCGAAGAAAACTTCAAGCGCCATTTaaatc                                                       >  8:15232/1‑136 (MQ=255)
                                                                                     aCATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGGGGTCGGTTGGGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTTCTCGATGTTATATTCAAGCGACATTTAAAGCTGttac                                                 >  6:328261/1‑138 (MQ=255)
                                                                                     aCATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                                                 <  5:184661/140‑1 (MQ=255)
                                                                                            ttGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGTCGGGTGGGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGCCGTACACGATGATCTCTTAAAGCGCCATTTAAAGCTCGTCCTGAta                                            >  8:508123/1‑137 (MQ=255)
                                                                                                  ttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCTGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt                                    <  1:228344/140‑1 (MQ=255)
                                                                                                   tAGTGTAGAGTCGGTTTTTGTACTTGTGGGGCGGTCGGGTGGGGATGAG‑GAAAGGTGTCGTGGCATAACCAGGCGGCCACACGGACGTACTCGATGCTCGCATCAAGCGACATTTCAATCTCATGCTGGtgttcgttt                                     >  8:118037/1‑136 (MQ=255)
                                                                                                     gtgtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc                                   <  7:499003/138‑1 (MQ=255)
                                                                                                     gtgtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                 <  3:523523/140‑1 (MQ=255)
                                                                                                       gtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                 <  7:286166/138‑1 (MQ=255)
                                                                                                                    ttGTACTTCTGGCGCTGTCGGTTGCTGATGAG‑TAAAGGTGTCGTTGCATACCAGCGCGGCAACACGTACGTACTCGATGATCTCTTCTAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGGCGTAACCCAAGTgag                  <  4:187599/140‑3 (MQ=255)
                                                                                                                    ttGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  1:133201/140‑1 (MQ=255)
                                                                                                                    ttGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  5:272201/140‑1 (MQ=255)
                                                                                                                    ttGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  1:689871/140‑1 (MQ=255)
                                                                                                                    ttGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  3:189886/140‑1 (MQ=255)
                                                                                                                    ttGTACTTCTGGCGCGGTCGGTTGCGGAGGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                    <  7:627169/138‑1 (MQ=255)
                                                                                                                    ttGTACTTCTGGCGCGGTCGGTTGCGGACGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  3:286567/140‑1 (MQ=255)
                                                                                                                      gTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  7:508123/138‑1 (MQ=255)
                                                                                                                      gTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                <  6:437824/140‑1 (MQ=255)
                                                                                                                      gTACTTCTGGCGCGGGCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcgct                <  3:176908/140‑2 (MQ=255)
                                                                                                                       tACTTCTGGCGCGGGCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACTGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcgc                 <  8:656044/138‑1 (MQ=255)
                                                                                                                           tCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt           >  3:624358/1‑140 (MQ=255)
                                                                                                                            cTGGCGCGGTCGGTTGGGGTTGAG‑TTAAGGGGTCGTGGGATTACAGCGCGGCTACTCGGTGGTACTCGATGATCTCTTCTAGCGACATTTCAAGCTCTTCCTGATCTTGGTCTTCGTCGTTCCCGATCTGCGCGATGTTa          <  4:581597/140‑1 (MQ=255)
                                                                                                                            cTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTa          <  1:76960/140‑1 (MQ=255)
                                                                                                                             tGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt           >  7:451937/1‑138 (MQ=255)
                                                                                                                             tGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATATTCGTCTTCGTCGTAACCCAACTGCGCGATGtt           >  7:201008/1‑138 (MQ=255)
                                                                                                                              ggCGCTGTAGGTTGCGGATGAG‑TAAAGTTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGTTTATCTCTTCAAGCGACATTTCAAGCTCTTCCTTATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACg        <  6:601054/140‑1 (MQ=255)
                                                                                                                              ggCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACg        <  5:328261/140‑1 (MQ=255)
                                                                                                                              ggCGCGGGCGGGTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACg        <  5:324826/140‑1 (MQ=255)
                                                                                                                                  gggtcggtTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCACGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa    <  3:64279/139‑1 (MQ=255)
                                                                                                                                  cggtcggtTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCa      <  7:161276/138‑1 (MQ=255)
                                                                                                                                    gtcggtTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtc  >  5:78462/1‑140 (MQ=255)
                                                                                                                                    gtcggtTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtc  >  2:5287/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                           
CGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATC  >  NZ_CP022686/1674111‑1674382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A