Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F0 I0 R1 81 326.4 10500568 93.1% 9776028 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 235,022 G→T 27.7% H68N (CAT→AAT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273235,0220GT27.7% 127.7 / 7.3 74H68N (CAT→AAT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (34/15);  new base T (8/16);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 5.29e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAA  >  CP009273/234879‑235169
                                                                                                                                               |                                                                                                                                                   
gACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAtt                                                                                                                                               <  2:4136785/150‑1 (MQ=255)
 aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAAATAAAAGAATAttt                                                                                                                                              >  1:3340348/1‑150 (MQ=255)
   aactggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAATAATATAAGAGTTAGCTGTTTTGTTATGTCTGTTGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATTTTTaa                                                                                                                                            <  1:859933/146‑1 (MQ=255)
   aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                                            <  1:860011/150‑1 (MQ=255)
   aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                                            <  1:860606/150‑1 (MQ=255)
   aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                                            <  1:4246157/150‑1 (MQ=255)
    aaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATATTAAGTAAAATTAAATGAATATTTaaa                                                                                                                                           >  1:3276629/1‑150 (MQ=255)
        gAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaatg                                                                                                                                       >  2:2524927/1‑148 (MQ=255)
            tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTTTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                   <  1:2121610/150‑1 (MQ=255)
              ttAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACg                                                                                                                                 <  2:5152191/150‑1 (MQ=255)
                  aGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAATGAAAAAGACAt                                                                                                                             >  1:2617391/1‑150 (MQ=255)
                             cGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACATGAAAACGAAGc                                                                                                                  >  1:5165760/1‑150 (MQ=255)
                             cGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAATAAAGATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGGAAAAAGACATTAAAACGCAGc                                                                                                                  >  1:5165753/1‑150 (MQ=255)
                                tAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTATAGTAAATAAAAATAAAATAATATTTAAAAATTAAAACGACAATAAACCCAAGcaaa                                                                                                               >  1:1266899/1‑148 (MQ=255)
                                    tattatTAAAAATATAAGTGTTTGCTTTTTTTTTTTGTCTGTGGCTAATTTTACTACCTTTTTTTTTTTTATTAATAATTATTTTTTTATCTTAATTAAATTTAAATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAAt                                                                                                           <  1:130405/150‑1 (MQ=255)
                                            aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATAGTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAATAAAAAtta                                                                                                   >  2:3226769/1‑149 (MQ=255)
                                             aaaTATATGATTTTGTTTTTTTTTTATTTCTGTGGCTAAATTTACTACCTTCTTTTTTTTTATTAATATTTATTTTATTATCTTAATTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATtct                                                                                                  <  2:4881095/150‑1 (MQ=255)
                                             aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATtct                                                                                                  <  2:2564543/150‑1 (MQ=255)
                                              aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAATAATTATTTTATTATAGTAAGTAAAAATAAAATAATATTTAAAAAAGAAAAACAAATTAAAACGAACCACAGAATCAACaatata                                                                                                 >  1:2773749/1‑145 (MQ=255)
                                                  taagttttAGCTTTTTTGTTTTGTCGGTGGCTAATTTGACTACCTTCTTTTTTTTTATTAATAATGATTTTTTTATCTTATTAAAAATTAAATCAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                             <  1:810124/144‑1 (MQ=255)
                                                    agagTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACCACAAGAAACCGAAGCACACAAAAAAAATTCTCCaaaaa                                                                                           >  2:3088598/1‑147 (MQ=255)
                                                    agagTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAAATACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCACCAATCa                                                                                           >  2:3091559/1‑150 (MQ=255)
                                                     ttgTTCGCTTTATTGTTTTGTCTGTGGCTAATTTGACTACCTTGTTTTTTTTTATTATTATTGTTTTTATTATCGTAATTAAAATTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCAt                                                                                          <  1:344076/148‑1 (MQ=255)
                                                      agTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTTATAATTATTTTATTATAGTAAGTAAAATTAAATTAAAATTTAAAAAATAAAAACACAAGAAAACGAAACACAGAAACAACAATCTACaaaaaaa                                                                                         >  1:4838678/1‑145 (MQ=255)
                                                      agTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAAAAAACATTATCCaaaaata                                                                                         >  2:3065914/1‑145 (MQ=255)
                                                          tggtgttttgtttTGTCTGTGGATAATTTGAATACCTTCTTTTTTTTTTTTAAGTTTGATTTTTTTATCTTATGTAAATTTAATTTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAat                                                                                     <  2:1519381/147‑1 (MQ=255)
                                                          aGCTTTATTTTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAat                                                                                     <  2:5133520/150‑1 (MQ=255)
                                                          aGCTGTTTTGTTTTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGATTGATTTTATTTTCGTAAGTAAATTTAAATGATTATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAat                                                                                     <  2:1519931/150‑1 (MQ=255)
                                                          aGCTGTATTGTTATTTCTGTGGCTAAATTGAATACATACTTTTTTTTGATTAATAATGATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAat                                                                                     <  2:2617391/150‑1 (MQ=255)
                                                          aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAat                                                                                     >  2:4332175/1‑150 (MQ=255)
                                                          aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAat                                                                                     >  2:4332192/1‑150 (MQ=255)
                                                          aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAat                                                                                     >  2:4331800/1‑150 (MQ=255)
                                                            ttGTATTGTTATTTATGTGGCTAAATTGACTACCTTGTTTTTTTTGATTAATAATGATTTTATTATCTTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                                   <  2:575134/149‑1 (MQ=255)
                                                            cTGTTTTTTTATGTCTGTGGCTAAATTGACTCCCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                                   <  2:3848420/150‑1 (MQ=255)
                                                               tATTGTTATGTCTGTTGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                                                >  2:2515846/1‑150 (MQ=255)
                                                                  tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGt                                                                             >  2:3853900/1‑150 (MQ=255)
                                                                  tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTACGt                                                                             >  2:3854354/1‑150 (MQ=255)
                                                                  tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTACGt                                                                             >  2:3853858/1‑150 (MQ=255)
                                                                  tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATAAACATTCTCCAAACATAAAAAATTTCCGt                                                                             >  2:3782161/1‑150 (MQ=255)
                                                                   gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTGTTAAGAATTATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAATTAAAACGACATGAAACCGAATCACAAAATAAAAAATAAACAATCAAAAAATATTTCCGTg                                                                            >  2:1311732/1‑150 (MQ=255)
                                                                   gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGGTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAATCAACATTCTCCAATAATAAAAAATTTACGTg                                                                            >  2:3953367/1‑150 (MQ=255)
                                                                   gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATCGGAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATACACCCATCATAAAAAAATTCCGTg                                                                            >  2:3000825/1‑150 (MQ=255)
                                                                      ttttgtgtgGCTAATTTTAATACTTTTTTTTTTTTTATTAATATTTTTTTTTTTATTTTAATTAAATTTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAg                                                                         <  1:4877831/145‑1 (MQ=255)
                                                                       tGTCTGTGGCGAAATTGACTACCTTCTTTTTTTTGTTTAAGATTTATTTTTTTATCGTAAGTAAATTTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGc                                                                        <  2:2755914/150‑1 (MQ=255)
                                                                          ttgtgGATAATTTGACTACCTTTTTTTTTTTTATTAATAATGTTTTTATTATCTTAATTAAAATTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                                     <  2:973812/149‑1 (MQ=255)
                                                                            ggggCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATAAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtttt                                                                   <  2:3340348/148‑1 (MQ=255)
                                                                             tgGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAAAGATTTTATTATAGTAAGTAAAATTACATTAATATTTAAAAATGAAAAAGACATGAAAACGAAGCCCAAAATCAACATTCTCCAATCATAAAATATTTACGTTGAGCATTtta                                                                  >  1:4718678/1‑150 (MQ=255)
                                                                             tgGCAAATTTCAATACCTTGGTTTTTTTTATTAATAATTATTTTATTATCTTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                                  <  1:2974026/150‑1 (MQ=255)
                                                                                   aaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCAAAGAAACAAAATTATACAATCAAAAAATATTTCCGTGGAGCATTTTATTATTg                                                            >  2:1179207/1‑150 (MQ=255)
                                                                                          tcccTTCTTTTTTTTTATTAAGAATGATTTTATTACCTTAAGAAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATag                                                     <  2:1363932/148‑1 (MQ=255)
                                                                                            ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATTAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                   >  1:604522/1‑150 (MQ=255)
                                                                                            ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                   >  1:3226568/1‑150 (MQ=255)
                                                                                                 gTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTa                                              >  1:2647554/1‑150 (MQ=255)
                                                                                                 gTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATATAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCAAATCATAAAATATTTCATTGGAGCATTTTATTATTGAATATAGAGGTTTa                                              >  1:2647956/1‑150 (MQ=255)
                                                                                                        tGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCgg                                       >  2:1662921/1‑150 (MQ=255)
                                                                                                           ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                    >  2:3892684/1‑150 (MQ=255)
                                                                                                                 attaTTTTATTATCTTAATTAAAATTACTTTAATATTAAAAAACTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACa                              <  1:3226769/147‑1 (MQ=255)
                                                                                                                  tGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATCGAGGTTTAACTCCGGTAAAAAACaa                             >  2:4251008/1‑150 (MQ=255)
                                                                                                                      tatattatCTTAAGTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                         <  2:4744963/148‑1 (MQ=255)
                                                                                                                        tattatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCTAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGc                       >  2:1337489/1‑150 (MQ=255)
                                                                                                                        tattatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATATAGGTTTAACTCCGGTAAAAAACAAAGAAGc                       >  2:3978142/1‑150 (MQ=255)
                                                                                                                        tattatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAAACCGGTAAAAAACAAATAAGc                       >  2:3642971/1‑150 (MQ=255)
                                                                                                                         attatCTTAAGTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCa                      <  2:3276629/150‑1 (MQ=255)
                                                                                                                         attatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaaaga                      >  1:4744963/1‑147 (MQ=255)
                                                                                                                          ttatCGTAAGTAAATTTACATGAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt                     <  2:2331417/150‑1 (MQ=255)
                                                                                                                           tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                    >  1:2439427/1‑150 (MQ=255)
                                                                                                                               gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt                >  1:4169289/1‑150 (MQ=255)
                                                                                                                               gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt                >  2:100229/1‑150 (MQ=255)
                                                                                                                                tattaaaaaTTAAATTAATATTTAAAATCGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATg               <  1:4903531/145‑1 (MQ=255)
                                                                                                                                  attaaAATTACATTAATATATAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa             <  2:1011041/148‑1 (MQ=255)
                                                                                                                                  agtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa             >  2:1741353/1‑150 (MQ=255)
                                                                                                                                   gtaaAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg            <  2:1266899/150‑1 (MQ=255)
                                                                                                                                           aaaTGAATATTAAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaat    <  1:1083428/148‑1 (MQ=255)
                                                                                                                                             aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataa  >  2:656145/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAA  >  CP009273/234879‑235169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A