Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F52 I0 R1 779 320.1 10410758 96.6% 10056792 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 235,022 G→T 27.2% H68N (CAT→AAT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273235,0220GT27.2% 315.2 / 25.0 188H68N (CAT→AAT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (72/53);  new base T (10/49);  total (83/105)
Fisher's exact test for biased strand distribution p-value = 2.10e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

CCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAAT  >  CP009273/234874‑235170
                                                                                                                                                     |                                                                                                                                                    
ccTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATg                                                                                                                                                      <  1:1662640/149‑1 (MQ=255)
 cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGa                                                                                                                                                     <  1:2460588/149‑1 (MQ=255)
    tgACAACAGCAGCTATAAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTATTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAata                                                                                                                                                  <  2:3498248/149‑1 (MQ=255)
    tgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGT‑AAAATTACATGAata                                                                                                                                                  >  2:517004/1‑149 (MQ=255)
       cAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATAttt                                                                                                                                               <  1:3422716/149‑1 (MQ=255)
        aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATTATTTTATTATATTAAGT‑AAAATTAAAATAATATTTa                                                                                                                                              >  1:474898/1‑149 (MQ=255)
        aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATAGTAAGT‑AAAATTAAAAGAATATTTa                                                                                                                                              >  1:1866006/1‑149 (MQ=255)
              aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAgg                                                                                                                                        <  1:3984633/149‑1 (MQ=255)
              aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAgg                                                                                                                                        <  1:1340441/149‑1 (MQ=255)
                cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGaa                                                                                                                                      <  1:2696073/149‑1 (MQ=255)
                 tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGaaa                                                                                                                                     <  2:2408602/149‑1 (MQ=255)
                   ttAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                                   <  2:4176536/149‑1 (MQ=255)
                        ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACAt                                                                                                                              >  2:2378091/1‑149 (MQ=255)
                             ttttACTATAGCTTTTATTAAAAATATATGTGTTAGCTGTATTTTTTTGTCTGTGGCTAAATTTACTACCTTCTTTTTTTTGATTAATAATGATTTTTTTATCTTAATT‑AAAATTAATTTAATATTTAAAAAGGAAAACGACATGAAAc                                                                                                                         <  1:3426334/147‑1 (MQ=255)
                                tACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTATCGTAATT‑AAAAATACATGAATATTTAAAAATTAAAAAGACATTAAAACGa                                                                                                                      >  1:5014466/1‑149 (MQ=255)
                                 actatatCTATTATAAAAAATATAAGTTTTAGCTTTTTTTTTATGTCTGTGGCTAAATTCAATACCTTCTTTTTTTTTATTAATATTGATTTTATTATCTTAATT‑AAATTTACATTAATATTTAAAAAGTAAAACGACATGAAACCGaa                                                                                                                     <  2:4373717/146‑1 (MQ=255)
                                 aCGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAATTTGACTCCCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAATTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGaa                                                                                                                     <  2:2601079/149‑1 (MQ=255)
                                    attgCTTTTATTAAAAATATAATTTTTAGCTTTATTTTTTTGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTTATTAATATTTATTTTATTATGGTAATT‑AAATTTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGca                                                                                                                  <  2:1890107/146‑1 (MQ=255)
                                      aGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAATAATGATTTTATTATAGTAAGT‑AAAATTAAAAGAAAATTTAAAAAGGAAAACGACAAGAAACCCAACcaca                                                                                                                >  1:2609731/1‑149 (MQ=255)
                                      aGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGT‑AAAATTACATGAATATTTAAAAATGAAAACGAAATTAAACCGAAGcacc                                                                                                                >  1:2345269/1‑148 (MQ=255)
                                      aGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTGTTTTTGATTAAGAATGATTTTATTATAGTAAGT‑AAAAATAAAAGAATATTTAAAAACGAAAAAGACAAGAAAACCAATcaca                                                                                                                >  1:2609715/1‑149 (MQ=255)
                                         ttttattaAAATTATAAGTGTTAGCTGTTTTGTTTTGTCTGTGGCTAATTTGACTACATTATTTTTTTTTTTTAATATTTATTTTTTTATCTTAAGT‑AAATTTAATTGATTATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGaa                                                                                                             <  1:3720080/147‑1 (MQ=255)
                                         tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTTTTATAGTAAGT‑AAAATTAAATTAATATTTAAAAAGGAAAACGAAATGAAACCGCAGCACAGaa                                                                                                             >  1:591675/1‑149 (MQ=255)
                                            ttttAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAATTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCa                                                                                                          <  1:1248301/146‑1 (MQ=255)
                                             ttaaaaaaTATATGTTTTCGCTGTTTTGTTTTGTGTGTGTCTAATTTGAATACCTTCTTTTTTTTTATTAATATTTTTTTTTTTATCGTAATA‑AAATTTAAATTAATATTTAAAAATTAAAACGACATGAAACCGAAGCACAGAATCaa                                                                                                         <  1:2234169/146‑1 (MQ=255)
                                             attAAAAATATATGTTTTTGCTGTATTGTTTTGTGTGGGGCTAAATTGACTACCTTCTTTTTTTTGATTAATAATTTTTTTATTATCGTAAGT‑AAAATTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCaa                                                                                                         <  1:2052852/147‑1 (MQ=255)
                                              gTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                                                        >  1:296506/1‑149 (MQ=255)
                                              gTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGACCAGAATCAAc                                                                                                        >  2:5131871/1‑149 (MQ=255)
                                              gTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGT‑AAAATTAAATGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAATAAAc                                                                                                        >  1:296556/1‑149 (MQ=255)
                                               tAAAATTATAAGTGTTTGGTGTTTTGTTTTGTGTGTGGCTAAATTTACTACGTTCGTTTTTTTGTTTAAGATTTTTTTTTTTATCGTAATT‑AAAATTACATTAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACa                                                                                                       <  1:4465282/149‑1 (MQ=255)
                                                  aaaTATAATTGTTTGTTTTTTTTTTTTGTCGGGGGCTAATTTGACTACCTTCTTTTTTTTTATAAATAATGTTTTTTTTATCTTAATT‑AAAATTAAATTAATATTTAAAAAGCAACACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                                    <  1:1508014/149‑1 (MQ=255)
                                                   aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTAAGT‑AAAATTAAATGAATATTTAAAAAGGAAAACGACATTAAAACGAAGCACAGAATCAACAttat                                                                                                   >  2:4441323/1‑147 (MQ=255)
                                                     tataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGT‑AAAATTACAATAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACACAAACAACAATCAcc                                                                                                 >  2:4848552/1‑149 (MQ=255)
                                                     tataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATAAAAGTAAAA‑AAAATTAAAATAATATTTAAAAAATAAAAAGAAATGAAAACGAATCACACAAacaacaatatca                                                                                                 >  2:4848379/1‑143 (MQ=255)
                                                      ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAAAGATTTTATTATCGTAAATAAAAATTAAAT‑AAAATTTAAAAAGGAAAACGAAAATAAAAAGAAGCACAGAAACAACAAtctcaa                                                                                                >  1:1131804/1‑147 (MQ=255)
                                                      ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCa                                                                                                >  2:2011237/1‑149 (MQ=255)
                                                       taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATAAACATTCTCaaa                                                                                               >  2:4997282/1‑149 (MQ=255)
                                                        aagttttAGCTTTTTTTTTTTTTGTGTGGCTAATTTGAATACCTTCTTTTTTTTTATTAAGATTTATTTTATTATCGTATTT‑AAAATTACTTGAATATTTAAAATGTAACACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                              <  1:3310205/144‑1 (MQ=255)
                                                        aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                              >  2:4742499/1‑149 (MQ=255)
                                                            gTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATa                                                                                          >  2:2851573/1‑149 (MQ=255)
                                                                   gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAtt                                                                                   >  2:1305770/1‑149 (MQ=255)
                                                                   gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGT‑AAAAATACATGAATATTTAAAAAGGAAAAAGACATGAAACCCAAGCACAGAATCAAAAATCACCAATAATAAAAAAtt                                                                                   >  1:2553016/1‑149 (MQ=255)
                                                                    tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATTATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                                  <  2:162673/149‑1 (MQ=255)
                                                                          tATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAAATTCTCCAATCATAAAATATTTCCGTgg                                                                            >  2:4131694/1‑149 (MQ=255)
                                                                           aTGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTGATTAAGAATTATTTTATTATCGTAATT‑AAAATTACATTAATATTAAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                           <  2:104012/149‑1 (MQ=255)
                                                                            tGTCTGTGGCGAATTTGACTACCTTCTTTTTTTTTATTAAGATTTATTTTATTATCTTAAGT‑AAATTTACATTAATTTTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAg                                                                          <  1:696555/149‑1 (MQ=255)
                                                                            tGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAg                                                                          >  2:3350506/1‑149 (MQ=255)
                                                                             gTCTGTGGCTAAATTTACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGc                                                                         <  2:4199034/149‑1 (MQ=255)
                                                                              tCTGTGGAGAAATTTACTACCTTCGTTTTTTTTATTAATATTTATTTTATTATCTTATGT‑AAAATTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCa                                                                        <  2:3300335/149‑1 (MQ=255)
                                                                                tgtgtataaaTTGACTACTTTTGTTTTTTTTATAAATATTTTTTTTATTATATTATTT‑AAATTAAATTTAATTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                                      <  1:3069323/142‑1 (MQ=255)
                                                                                tgtgGCTAAATTGAATACCTTCTTTTTTTTTATAAATAATGATTTTATTATCTTAATT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                                      <  2:3909783/149‑1 (MQ=255)
                                                                                tgtgGCGAAATTGACTACCTTCGTTTTTTTTATTTAGAATTATTTTTTTATAGTAAGT‑AAAAATAAAATAATAATTAAAAAATAAAAACACAATAAAACCAATCACACAAAAAAAAATATACAAAAAAAAAAAATTTAAGTGGAGCAtt                                                                      >  1:956341/1‑149 (MQ=255)
                                                                                tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAACATAAAATATTTCCGTGGAGCAtt                                                                      >  1:5170536/1‑149 (MQ=255)
                                                                                tgtgGCGAAATTGACTACATTCTTTTTTTTGATTAAGAATTATTTTTTTATCTTAATT‑AAAATTAAATTAATTTTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                                      <  1:2494092/149‑1 (MQ=255)
                                                                                  tgGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtttt                                                                    <  2:3526235/149‑1 (MQ=255)
                                                                                  tgGCGAAATTGACTACCTTCGTTTTTTTGAATAAGAAATAATTTATTATACTAAGT‑AAAAATAAAATAATATATAAAAATGAAAACGAAAATAAACAGAAGCAAACAAACAAAATTCTCCAAAAATAAAATATTTACGAGGAGCAtttt                                                                    >  2:22784/1‑149 (MQ=255)
                                                                                  gggCTAATTTTACTACCTTCGTTTTTTTGATTAAGATTTATTTTATTATCGTAAGT‑AAATTTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtttt                                                                    <  2:929112/148‑1 (MQ=255)
                                                                                     ctaaaTTGACTACATTCTTTTTTTTTATTAATAATTATTTTATTATCTTAAGT‑AAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                                 <  2:2528773/147‑1 (MQ=255)
                                                                                        aaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                              <  2:765895/149‑1 (MQ=255)
                                                                                           ggACTACCTTCTTTTTTTTTATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                           <  2:4982522/148‑1 (MQ=255)
                                                                                               tACCTTCGTTTTTTTTATTAAGAATGATTTTATTATAGTAAGT‑AAAATTACAAGAATATTTAAAAATGAAAAAGACAAGAAACCGAAGCACAGAATCAACAATATCCAATCATAAAAAATTTAAGTGGAGCATTTTATTATTTAAtata                                                       >  2:2351853/1‑149 (MQ=255)
                                                                                                  cTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATT‑AAAATTACATGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                    <  2:1131804/149‑1 (MQ=255)
                                                                                                   ttCTTTTTTTTGATTAAGAATTATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAgg                                                   <  2:1578921/149‑1 (MQ=255)
                                                                                                      tttttttttATTAACAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                                <  2:1494623/148‑1 (MQ=255)
                                                                                                      gTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                                >  1:4656318/1‑149 (MQ=255)
                                                                                                      gTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                                <  2:810072/149‑1 (MQ=255)
                                                                                                      gTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                                >  2:2668991/1‑149 (MQ=255)
                                                                                                       tttttttGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTa                                               >  2:2545755/1‑149 (MQ=255)
                                                                                                         ttttttATTAATATTTATTTTATTATCGTAATT‑AAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                             <  1:1105953/149‑1 (MQ=255)
                                                                                                         ttttttATTAATAATTTTTTTATTATATTAATT‑AAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                             <  1:5057543/149‑1 (MQ=255)
                                                                                                                ttAAGAATTTTTTTTTTATCGTAATT‑AAAATTACATTAATTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                                      <  1:2901294/149‑1 (MQ=255)
                                                                                                                 tAAGAATTATTTTATTATCGTATTT‑AAAATTAATTTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                     <  1:5178687/149‑1 (MQ=255)
                                                                                                                  aaGAATGATTTTATTATCTTAATA‑AAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                                    <  1:4024023/149‑1 (MQ=255)
                                                                                                                  aaGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                                    >  2:2820152/1‑149 (MQ=255)
                                                                                                                      ttGATTTTTTTATCTTAAGT‑AAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                                <  1:618048/148‑1 (MQ=255)
                                                                                                                      aTGATTTTATTACCGTAATT‑AAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                                <  1:816407/149‑1 (MQ=255)
                                                                                                                       ttaTTTTATTATGTTATTA‑AAAATTACATTAATATTTAAAAATCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACa                               <  1:3563740/147‑1 (MQ=255)
                                                                                                                          ttttATTATCTTAATT‑AAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                            <  2:1963946/149‑1 (MQ=255)
                                                                                                                               ttatCGTAATT‑AAAATTAATTTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCa                       <  1:2281108/149‑1 (MQ=255)
                                                                                                                                 atCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                     >  2:4666602/1‑149 (MQ=255)
                                                                                                                                 atCGTAAGT‑AAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                     <  2:2915541/149‑1 (MQ=255)
                                                                                                                                  tCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTg                    >  2:1777223/1‑149 (MQ=255)
                                                                                                                                    gtaagt‑aaATTTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa                  <  2:601575/149‑1 (MQ=255)
                                                                                                                                     taatt‑aaATTTAATTTAATTTTTAAAATTTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt                 <  1:4441323/149‑1 (MQ=255)
                                                                                                                                     taagt‑aaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAATCAAAGAAGCCTTGAAt                 >  2:3510754/1‑149 (MQ=255)
                                                                                                                                     taagt‑aaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt                 >  1:486578/1‑149 (MQ=255)
                                                                                                                                      aagt‑aaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATg                >  1:4640500/1‑149 (MQ=255)
                                                                                                                                         t‑aaaataaaaTTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg             <  2:2609731/149‑1 (MQ=255)
                                                                                                                                         t‑aaAATTAAATTAATATTAAAAAAGAAACACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg             <  1:905415/149‑1 (MQ=255)
                                                                                                                                         t‑aaAATTAAATGAATATTTAAAAATTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg             <  2:1499043/149‑1 (MQ=255)
                                                                                                                                           aaaaTTAAATTAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAgg            <  1:3291788/149‑1 (MQ=255)
                                                                                                                                             aaTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGa          >  1:427287/1‑149 (MQ=255)
                                                                                                                                              attaaattaaTATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaa         <  1:3216952/149‑1 (MQ=255)
                                                                                                                                              aTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaa         >  1:301875/1‑149 (MQ=255)
                                                                                                                                                tACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaa       >  2:4478833/1‑149 (MQ=255)
                                                                                                                                                 aCATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaaa      <  2:134659/149‑1 (MQ=255)
                                                                                                                                                  cATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaat     >  2:3842161/1‑149 (MQ=255)
                                                                                                                                                   aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAGCAAAGAAGCATTGAATGCAGGGAAAaata    >  1:3507460/1‑149 (MQ=255)
                                                                                                                                                   aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaata    >  1:3507212/1‑149 (MQ=255)
                                                                                                                                                     gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGAAGGGAAAaataat  >  2:4457189/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGT‑AAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAAT  >  CP009273/234874‑235170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A