Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F17 I0 R1 93 279.2 9183790 95.6% 8779703 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 235,022 G→T 27.3% H68N (CAT→AAT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273235,0220GT27.3% 423.7 / 34.5 246H68N (CAT→AAT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (113/51);  new base T (17/60);  total (131/115)
Fisher's exact test for biased strand distribution p-value = 8.58e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

CCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAA  >  CP009273/234874‑235169
                                                                                                                                                     |                                                                                                                                                   
ccTGTGACAACAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATg                                                                                                                                                     <  1:3893455/149‑1 (MQ=255)
  tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGaa                                                                                                                                                   <  2:3769209/149‑1 (MQ=255)
    tgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAATTAAAATT‑AAATGAata                                                                                                                                                 >  1:661410/1‑149 (MQ=255)
        aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAATAATTATTTTATTATAGTAATTAAAAAT‑AAATGAATATTTa                                                                                                                                             >  1:2685150/1‑149 (MQ=255)
        aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTATAGTAATTAAAATT‑AAAAGAATATTTa                                                                                                                                             >  1:1247087/1‑149 (MQ=255)
         aaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTaa                                                                                                                                            <  2:558510/149‑1 (MQ=255)
          aaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAATAATTATTTTATTATAGTAATTAAAATT‑AAATGAATATTTaaa                                                                                                                                           >  1:1436721/1‑149 (MQ=255)
          aaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTaaa                                                                                                                                           <  1:2801227/149‑1 (MQ=255)
            ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTaaaaa                                                                                                                                         <  2:3304623/149‑1 (MQ=255)
              aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTTATAATTATTTTATTATAGTAATTAAAATT‑AAATGAATTTTTAAAAAgt                                                                                                                                       >  1:2516862/1‑148 (MQ=255)
              aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTTAGTAAAATT‑AAATGAATATTTaaaaatt                                                                                                                                       >  2:2677215/1‑147 (MQ=255)
              aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAgg                                                                                                                                       <  1:3506130/149‑1 (MQ=255)
                cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAATAATGATTTTATTATAGTAAGTAAAAAT‑ACAATAATATTTAAAAATGaa                                                                                                                                     >  1:500920/1‑149 (MQ=255)
                  aTTATAGGTGCTATTACTATAGCTTTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAATATT‑ACATGAATATTTAAAAAGGaata                                                                                                                                   <  1:68375/149‑3 (MQ=255)
                  aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGaaaa                                                                                                                                   <  1:2975740/149‑1 (MQ=255)
                   ttAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAAc                                                                                                                                  <  2:2058837/149‑1 (MQ=255)
                     aaaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGGTTAATAATTATTTTATTATCGTAAGTAAAAAT‑AAAATAATATTTAAAAAGGaaaaacg                                                                                                                                >  1:4444113/1‑146 (MQ=255)
                       tggTGCTATTACGATAGCTATTATTAAAAATATAAGAGTTAGCTGTTTTGTTTTGTCTGTGGCTAATTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCTTAATTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACa                                                                                                                              <  1:1875422/148‑1 (MQ=255)
                       aGGTGCTATTACGATTGGTATTAGTAAAAATATAATAGTTAGCTGTATTGTTATTTCTTTTGCGAAATTGACTACATTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACa                                                                                                                              <  2:4034937/149‑1 (MQ=255)
                       aGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACa                                                                                                                              >  2:1835900/1‑149 (MQ=255)
                       aGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACa                                                                                                                              >  2:618670/1‑149 (MQ=255)
                        ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATGATTTTATTATAGTAAGTAAAAAT‑ACAAGAATATTTAAAAATTaaaaaaaaaa                                                                                                                             >  1:819146/1‑143 (MQ=255)
                              aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAATGAAAACGACATGAAAcc                                                                                                                       >  2:2727910/1‑149 (MQ=255)
                                  cGATAGATATTATTAAAAATATATGTGTTAGCTGTATTGTTATGTCTGTGGCTAATTTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACTTGAATATTTAAAAAGTAAAACGACATGAAACCGAAg                                                                                                                   <  2:239814/149‑1 (MQ=255)
                                    aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTTATAATTATTTTTTTTTATTAAGTAAAAAT‑AAATTAAAATTTAAAAATGAAAACGACAATAAAACGAAGca                                                                                                                 >  1:1524591/1‑149 (MQ=255)
                                     tatattttAGAAAAAATATTTGTGTTTTTTGTATTTTTATGTTTTTGGCTAATTTTACTTCTTTTTTTTTTTTTATTAATATTTTTTTTTTTATCTTAAGTAAAATT‑AAATTAATATTAAAAAAGTAAAACGACATGAAACCGAAGcac                                                                                                                <  2:3462957/143‑1 (MQ=255)
                                      aGCTATTTGAAAAAATATATGTGTTCGGTTTTTTGTTTTGTCGGTGGCTAAATTGACTACATTCTTTTTTTTTATTAAGATTTATTTTATTATGTTATGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcaca                                                                                                               <  1:2619156/149‑1 (MQ=255)
                                       gCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAATAATGATTTTATTATAGTAAATAAAATT‑AAATGAATATTTAAAAATGAAAACCACAAGAAACCGAAGcacat                                                                                                              >  2:3108771/1‑148 (MQ=255)
                                       gCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAAAATTTAAAAAGGAAAACGACATGAAACCGAAGcacac                                                                                                              >  2:3699182/1‑148 (MQ=255)
                                           ttAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATCGTAAGTAAAATT‑AAATGAATATTTAAAAAGGAAAACGACATTAAACCGAATCACAGAATc                                                                                                          >  2:100313/1‑149 (MQ=255)
                                               tAAAAATATTTGTTTTAGCTTTTTTGTTTTTTCTGTGGTTAATTTGATTACTTTCTTTTTTTTGTTTTTGATTGTTTTTTTTTTCGTAAGTAAATTT‑ATATTAATTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACa                                                                                                      <  2:528810/149‑1 (MQ=255)
                                               tAAAAATATATGTGTTAGCTTTTTTTTTATGTCTGTGGCTAATTTTAATACATTCTTTTTTTTGATTAATAATTATTTTATTATGTTAATTAAAATT‑AAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACa                                                                                                      <  2:935089/149‑1 (MQ=255)
                                               tAAAAATATAAGAGTTAGCTGTATTGTTTTGTGTGTGGCGAATTTGACTACCTTCGTTTTTTTGATTAAGATTTATTTTTTTATCGTATGTAAAATT‑ACATGAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACa                                                                                                      <  2:2822899/149‑1 (MQ=255)
                                                aaaaaTTTTTGAGTTCGCTTTTTTTTTTTGTCGGTGGCTAATTTGACTACCTTGGTTTTTTTGATTAAGATTGATTTTATTATCGTAATTAAATTT‑ACTTGAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                                                     <  1:4045594/149‑1 (MQ=255)
                                                aaaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTGTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACCTGAAACCCCAGCACAGAATCAACaa                                                                                                     >  1:140177/1‑148 (MQ=255)
                                                    atatAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAATATTTTATTATCGTAAGTAAAATT‑AAAAGAATATTTAAAAAGGAAAAACACAAGAAACCCAAGCACAGAATCAACaaaaac                                                                                                 >  1:1723812/1‑144 (MQ=255)
                                                      ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACAATCTCCa                                                                                               >  2:1402027/1‑149 (MQ=255)
                                                      ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATTAAACCGAATCACAGAATCAACATTCTCCa                                                                                               >  2:129739/1‑149 (MQ=255)
                                                      ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCa                                                                                               >  2:2997777/1‑149 (MQ=255)
                                                       taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTACaa                                                                                              >  2:315297/1‑149 (MQ=255)
                                                          tatttAGTTGTATTTTTATGGTTGTGGCTAATTTGACTACCTTTTTTTTTTTTATTAATATTTTTTTTTTTTTCTTAATTAAATTT‑AATTTAATTTTTAAAACGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCa                                                                                           <  1:3133612/146‑1 (MQ=255)
                                                           agTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAATCTCCAATCAt                                                                                          >  2:2016064/1‑149 (MQ=255)
                                                                  tttATTTTTTTTTCTGTGGCGAATTTGACTACCTTTTTTTTTTTTTTTAATATTTTTTTTATTATCTTATTTAAATTT‑AATTTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                                   <  1:3516397/147‑1 (MQ=255)
                                                                   gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATTATTTTATTATAGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAAACCAAGCACAGAAAAAACAATATCCAATAAAAAAATAtt                                                                                  >  1:1014303/1‑149 (MQ=255)
                                                                   gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATTATTTTATTATAGTAAGTAAAATT‑AAATTAATATTTAAAAATGAAAAAGAAATGAAAACGAAGCACAGAATAAAAATTCTACAATAATAAAAAAtt                                                                                  >  1:3714054/1‑149 (MQ=255)
                                                                    tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                                 >  2:330015/1‑149 (MQ=255)
                                                                     aTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTATCGTAAGTAAAATT‑AAATGAAAATTTAAAAAGGAAAACGAAATGAAAACGAAGCACAGAATCAACAATCTCCAAACATAAAATAttta                                                                                >  1:356046/1‑148 (MQ=255)
                                                                      tttttttgtgtgtgGCTAATTTTACTACATTGTTTTTTTTTTTTAATAATTTTTTTTTTATCTTATTAAAATTT‑AATTTATTATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTcc                                                                               <  1:1004750/143‑1 (MQ=255)
                                                                      ttGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATAGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATAAACAATATCCAATAATAAAAAAtttac                                                                               >  1:2144034/1‑147 (MQ=255)
                                                                         ttATGTCTGTGGCTAATTTTACTACCTACTTTTTTTTTTTTAAGATTTTTTTTTTTATCGTAATTAAAATT‑ACATCAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                                            <  2:3894943/149‑1 (MQ=255)
                                                                           aTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑AAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATAAACAATCTCCAATCATAAAATATTTACGTgta                                                                          >  2:3610119/1‑147 (MQ=255)
                                                                              tCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCa                                                                       >  2:4094246/1‑149 (MQ=255)
                                                                                 gtgGCTAAATTGAATACATTTTTTTTTTTTTTTAATAATTATTTTATTATCTTAA‑TAAAATTAACATTAATATTAAAAAAGAAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAttt                                                                    <  1:913664/149‑1 (MQ=255)
                                                                                 gtgGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTTTAGTAAGTAAAATT‑AAAGGAATATTTAAAAAAGAAAACGACAAGAAACCCAAGCACACAATAAACAATATCCAATCAAAAAATATTTAAGTGGGGCCttt                                                                    >  1:1603593/1‑149 (MQ=255)
                                                                                 gtgGCCAAATTCACTACCTTCTTTTTTTTTATTAATAATTATTTTATTATCTTAATAAAAATA‑AAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAttt                                                                    <  1:1828089/149‑1 (MQ=255)
                                                                                           gtaCTACCTTCTTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATT‑ACTTGAATATTTAAAATGGAACACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                          <  1:3439298/147‑1 (MQ=255)
                                                                                             aatACCTTCTTTTTTTTTTTTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAata                                                        <  1:2233811/147‑1 (MQ=255)
                                                                                             aCTCCCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTATGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAata                                                        <  2:2354320/149‑1 (MQ=255)
                                                                                                 ccTTCGTGTTTTTGATTAATAAAGATTTTAATATAGTAATTAAAAAT‑AAAAGAATATATAAAAAAGAAAACGACCAGAAAACCCAGCACAGAAAAAAAAATATCAAATCAAAAAATAATTACGTGGAGCATTTTATTATTGAAAATaga                                                    >  2:2101101/1‑149 (MQ=255)
                                                                                                 acttcttTTTTTTGATTAATATTTATTTTTTTCTTTAAATTAAATTT‑AATTTAATTTTAAAAAAGCAACACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATaga                                                    <  1:1930795/148‑1 (MQ=255)
                                                                                                  cTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                   >  1:760699/1‑149 (MQ=255)
                                                                                                    tCGTTTTTTTTATTAATAATTATTTTATTATCTTAATTAAAATT‑ACATTAAAATTAAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                                 <  1:3553746/149‑1 (MQ=255)
                                                                                                    tCGTTTTTTTGATTAAGAATGATTTTATTATCTTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                                 <  2:1771582/149‑1 (MQ=255)
                                                                                                    tCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                                 >  1:2535758/1‑149 (MQ=255)
                                                                                                      tttttttttATTAATATTTTTTTTATTATCTTAAGTAAAATT‑ACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                               <  1:3328136/148‑1 (MQ=255)
                                                                                                      ttttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                               <  2:156141/148‑1 (MQ=255)
                                                                                                      gTTTTTTTGTTTAATAATGTTTTTATTATCGTAAGTAAAATT‑ACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                               <  1:752897/149‑1 (MQ=255)
                                                                                                         tttttGTTTATTATTTATTTTATTATCTTAATTAATTTT‑AATTTAATTTTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                            <  1:4525658/149‑1 (MQ=255)
                                                                                                         tttttGATTAATATTTTTTTTATTATCGTAAGTAAAATT‑AAATTAATATTAAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                            <  1:3706106/149‑1 (MQ=255)
                                                                                                         tttttGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                            >  2:1161682/1‑149 (MQ=255)
                                                                                                           tttGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                          >  2:4454351/1‑149 (MQ=255)
                                                                                                           tttGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                          >  2:478269/1‑149 (MQ=255)
                                                                                                              taTTAATAATTATTTTTTTATCGTATTTAAAATT‑AAATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCgg                                       <  1:1779576/148‑1 (MQ=255)
                                                                                                                ttAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                                     >  1:3310914/1‑149 (MQ=255)
                                                                                                                ttAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                                     >  2:1366551/1‑149 (MQ=255)
                                                                                                                   aGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa                                  >  2:1904580/1‑149 (MQ=255)
                                                                                                                     aaTTATTTTATTATCTTAATTAAAATT‑AAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                                <  1:2581863/149‑1 (MQ=255)
                                                                                                                      aTGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                               >  1:1989774/1‑149 (MQ=255)
                                                                                                                       tGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACa                              >  1:1261297/1‑149 (MQ=255)
                                                                                                                           ttttttATCTTAATAAAAATT‑AAATTAATAATAAAAAATCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                          <  1:2677215/149‑1 (MQ=255)
                                                                                                                               ttatCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCa                      >  2:2366676/1‑149 (MQ=255)
                                                                                                                                ttttgtaattaaATTT‑AATTTAATTTTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt                     <  1:4299409/145‑1 (MQ=255)
                                                                                                                                tatCTTAATTAAAATT‑ACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt                     <  2:1247136/149‑1 (MQ=255)
                                                                                                                                tatCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACTACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCAGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGTTAAAAAACAAAGAAGCAt                     >  1:4020605/1‑149 (MQ=255)
                                                                                                                                 atCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                    >  1:2429446/1‑149 (MQ=255)
                                                                                                                                  tCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTg                   >  1:4163188/1‑149 (MQ=255)
                                                                                                                                   cGTAATTAAAATT‑ACTTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGa                  <  2:1723812/149‑1 (MQ=255)
                                                                                                                                    ttaagtaaAATT‑AAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa                 <  2:1436898/148‑1 (MQ=255)
                                                                                                                                    gaaattaaAATT‑ACATCAATATTAAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa                 <  1:2606823/144‑1 (MQ=255)
                                                                                                                                       agtaaAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGc              >  1:4136937/1‑149 (MQ=255)
                                                                                                                                         aaaaaTT‑AAATGAATATATAAAAAGGAACACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg            <  1:1608050/148‑1 (MQ=255)
                                                                                                                                          aaaaTT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAgg           >  1:592723/1‑149 (MQ=255)
                                                                                                                                          aaaaTT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAgg           >  1:3095328/1‑149 (MQ=255)
                                                                                                                                          aaaaTT‑AAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAgg           <  1:3185310/149‑1 (MQ=255)
                                                                                                                                           aaaTT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAggg          >  2:3428722/1‑149 (MQ=255)
                                                                                                                                             aTT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaa        >  1:4118344/1‑149 (MQ=255)
                                                                                                                                                 aCATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaaa     >  1:3414080/1‑149 (MQ=255)
                                                                                                                                                    tGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACATAATCAACATTCTCCAATCATAAAATATTTCCTTGGAGCATTTTATTATTGAATATAGAGGTTTATCTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataa  >  1:3803048/1‑149 (MQ=255)
                                                                                                                                                    tGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataa  >  1:4520289/1‑149 (MQ=255)
                                                                                                                                                    tGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataa  >  1:4412134/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATT‑ACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAA  >  CP009273/234874‑235169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A