Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F368 I0 R1	1472	214.4	7116750	95.9%	6824963	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	161,068	A→C	38.0%	intergenic (+47/‑149)	hrpB → / → mrcB	putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	161,068	0	A	C	38.0%	22.0 / 11.4	41	intergenic (+47/‑149)	hrpB/mrcB	putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
Reads supporting (aligned to +/- strand): ref base A (13/12); new base C (5/11); total (18/23)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGC  >  CP009273/160922‑161205

tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt                                                                                                                                          >  1:2857770/1‑148 (MQ=255)

  aGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                                        >  2:1907360/1‑148 (MQ=255)

   ggTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTccg                                                                                                                                       >  2:325252/1‑146 (MQ=255)

         aaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCa                                                                                                                                 >  1:1850728/1‑148 (MQ=255)

          aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCAc                                                                                                                                >  1:2015765/1‑148 (MQ=255)

           aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACg                                                                                                                               >  2:3275959/1‑148 (MQ=255)

           aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg                                                                                                                               >  2:149567/1‑148 (MQ=255)

           aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg                                                                                                                               >  2:2801639/1‑148 (MQ=255)

           aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg                                                                                                                               >  2:719010/1‑148 (MQ=255)

            agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGt                                                                                                                              >  2:590245/1‑148 (MQ=255)

              agaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACgggg                                                                                                                            >  2:1405105/1‑145 (MQ=255)

              agaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgg                                                                                                                            >  2:214515/1‑146 (MQ=255)

                   aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTgcgcccg                                                                                                                       >  1:2336427/1‑147 (MQ=255)

                      ggggtttAACCAAAAAATGTGGGGCGGTACGACCCGCAAAATCCGCCACCGACGCGAGGTACAAAAATTTATTCTTATGTTTGGTTTACACCCTCTCGGTGTGGGTGTGGGCGGGGTTGTGGGCCTCAGCGCGCACGTtcaccctcac                                                                                                                    <  2:3515316/143‑1 (MQ=255)

                                   aaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCGCGCCCCAAcccccgcc                                                                                                       >  2:880366/1‑143 (MQ=37)

                                   aaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCCCACCCAAAaccaaaac                                                                                                       >  2:880235/1‑143 (MQ=37)

                                      aTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGGCCCCTCCCCCCCACCCACTccccc                                                                                                    >  2:2229048/1‑146 (MQ=37)

                                       tGTCTGGCCGGACGCCCGGGAAAATCCTGCCCCCCCGCCACGCACTAAAAATAATGCTTAAGGTTGTTTTTCCCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa                                                                                                   <  2:296453/148‑1 (MQ=21)

                                                    gacCCGTCAAATAGGCCCCCGACGCGACTGACCAAAATTTATACGTATGTTGGGTTTCCTCCCACCCCGGGGGGGACCGGCCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGAc                                                                                      <  2:91913/148‑1 (MQ=255)

                                                                 cgccACCGACGCGCCGGACGAAAAAGTATGCCTACGGTGGGTTTTCTCCCTCCCCGTGGGGGCTAGGGCGGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACaa                                                                         <  2:3365775/145‑1 (MQ=255)

                                                                  ggCACCGCCGCGCGGGACTAAAAAGTATTCTTAAGGTTGTTTTTCTCCCTCTCCGTGTGGGAGAGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACaaa                                                                        <  2:3163081/147‑1 (MQ=255)

                                                                    cACCGCCGGGAGGGACTAAAATGTTTTGGTATGTTTGTTTTTAGCCCTCTCCGGGTGGGAGCGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtt                                                                      <  2:2336427/148‑1 (MQ=255)

                                                                         ccgccacgCACCAAAATGTTTTCTAAAGTTTGTTTTACACCCTCACCCTGTGGGAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag                                                                 <  2:162130/147‑1 (MQ=255)

                                                                            cgcgggccgAAAAAGTTTTCGTAGGGTTGGTTTTCTCCCTCTCCGTGTGGGAGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagaga                                                              <  2:2015765/141‑1 (MQ=255)

                                                                              acggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACTCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGata                                                            >  2:2360570/1‑148 (MQ=255)

                                                                               cggactAAAATGTATGCCAACGGTTGTTTTCCCCCCACTCCCTGTGGTAGCGGCCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat                                                           <  2:458830/148‑1 (MQ=255)

                                                                                        aattataCGTCAGGTTGGTTGGCGCCCGCGCCCGGGGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCGCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGAGATCTTCTTCTg                                                  <  2:3043336/145‑1 (MQ=255)

                                                                                              gcgtttggttggttTGCTCCCTCTCCGTGGGGTAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGCTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                                            <  2:859429/142‑1 (MQ=255)

                                                                                                 aaaGGTTTGTTTGCTCCCACCCCCGGGGGGGGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAAc                                         <  2:488965/147‑1 (MQ=255)

                                                                                                  atgtttgtttTTCGCCCTCCCCCTGTGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa                                        <  2:222579/144‑1 (MQ=255)

                                                                                                   aGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag                                       <  2:3346417/148‑1 (MQ=255)

                                                                                                    ggttggttTTCGCCCTCGCCCGGGGGGTGTGGGCGGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga                                      <  2:1754320/148‑1 (MQ=255)

                                                                                                       tggttTTCTCCCAGCCCCGGTGGGACCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga                                   <  2:1880161/148‑1 (MQ=255)

                                                                                                                         tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCCCCCCCCAAACCCCAACCCCCAGCGGGGGGGGGCCGGGCGCGCACACAAATTGAGAGATATGTTATTATGTTGTGGAACAGAAGGACACCCAATCgggggttt                 >  2:3382786/1‑143 (MQ=255)

                                                                                                                            gggggggggCGGGGGTGCGCCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcg              <  1:2054024/142‑1 (MQ=255)

                                                                                                                            gggTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcg              <  2:243195/148‑1 (MQ=255)

                                                                                                                               ggcggcccGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt           <  1:1848739/142‑1 (MQ=255)

                                                                                                                                     ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAatat     <  2:2082566/148‑1 (MQ=255)

                                                                                                                                       ggggTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCGAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTg   <  2:1747363/148‑1 (MQ=255)

                                                                                                                                        gggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc  <  2:1383025/148‑1 (MQ=255)

                                                                                                                                        gggTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc  <  2:1383053/148‑1 (MQ=255)

TGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGC  >  CP009273/160922‑161205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A