Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 161,068 A→C 24.2% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273161,0680AC24.2% 64.0 / 8.7 65intergenic (+47/‑149)hrpB/mrcBputative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (20/28);  new base C (5/12);  total (25/40)
Fisher's exact test for biased strand distribution p-value = 5.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAG  >  CP009273/160922‑161215
                                                                                                                                                   |                                                                                                                                                   
tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt                                                                                                                                                    >  2:3133564/1‑148 (MQ=255)
tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCAAAACA‑TGTCTGGCCGGACGACCCGGCAATTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGTTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCAt                                                                                                                                                    <  2:472359/148‑1 (MQ=255)
  aGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                                                  >  1:503397/1‑148 (MQ=255)
     tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcg                                                                                                                                               >  2:3446110/1‑148 (MQ=255)
         aaaaGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCa                                                                                                                                           >  1:100947/1‑148 (MQ=255)
         aaaaGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCa                                                                                                                                           >  2:2828895/1‑148 (MQ=255)
         aaaaGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCa                                                                                                                                           >  2:1396164/1‑148 (MQ=255)
         aaaaGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCa                                                                                                                                           >  2:1395412/1‑148 (MQ=255)
          aaaGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCAc                                                                                                                                          >  1:1802009/1‑148 (MQ=255)
          aaaGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCAc                                                                                                                                          >  1:535090/1‑148 (MQ=255)
          aaaGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCAc                                                                                                                                          >  1:535140/1‑148 (MQ=255)
          aaaGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCAc                                                                                                                                          >  1:809031/1‑148 (MQ=255)
           aaGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACg                                                                                                                                         >  1:448349/1‑148 (MQ=255)
            agagATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGt                                                                                                                                        >  1:2691180/1‑148 (MQ=255)
               gaTGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGtgga                                                                                                                                     >  2:3450914/1‑145 (MQ=255)
                aTGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACgggggc                                                                                                                                    >  1:3782868/1‑143 (MQ=255)
                aTGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgggg                                                                                                                                    >  1:3005020/1‑144 (MQ=255)
                  gAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCAACTCCGTGTGGGTGAGGGCCGGGGTGAGGGCCGCGGCGCGCCCgggggggg                                                                                                                                  >  1:1621870/1‑141 (MQ=255)
                   aaaGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtggggccc                                                                                                                                 >  2:981558/1‑141 (MQ=255)
                   aaaGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgggcccc                                                                                                                                 >  1:2460614/1‑141 (MQ=255)
                      gggCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggaccccccc                                                                                                                              >  2:919884/1‑144 (MQ=255)
                            atagccAAACATTGTGTGGCGGCACGACCCGCAAAAAACGCCCCCGCCGGGCGGGACTAAAATTTTTTCTTATGGTTGTTTTACGCCCTCGCCGTGGGGGAGGGGCCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTaa                                                                                                                         <  2:2741609/144‑1 (MQ=255)
                             aTCCCAACCA‑GGTCTGGCCGCCCGCCCCGGCAAATCCTGCACCGACGCGACGCACGAAAATGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAAcc                                                                                                                       <  2:2011786/148‑1 (MQ=37)
                              tCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCGCCCCCACCCCAAacc                                                                                                                      >  2:3576769/1‑148 (MQ=38)
                                  aaaCA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCGCACCCCAAaccacac                                                                                                                  >  2:1984326/1‑144 (MQ=37)
                                  aaaCA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCGCACCCCAAcccacac                                                                                                                  >  2:209161/1‑144 (MQ=37)
                                                        ccgcaaaaTCCTGCACCGCCGCGACTGACTAAAATTTATTGTTATGTTTGTTTTACACCCTCTCCGTGTGGGTGTGGGCGGGGGTGGGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGa                                                                                             <  2:1879540/143‑1 (MQ=255)
                                                        ccGGCAAATACTCCACCGCCGCGACGTACTAAAAAGTTTTCGTAAGGTTGGTTTTCTCCCTCTCCCTTTGTGTGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGa                                                                                             <  2:1817385/148‑1 (MQ=255)
                                                               acacgccACCGCCGCGCCGGACGAAAATGTATGCGTACGGTGGGTTTGCGCCCGCCCCCGGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                                                      <  2:1679751/142‑1 (MQ=255)
                                                                    ccccctccgcgacgTAATAAAATTTTTTCTTATGGTTGTTTTCCCCCCTCCCGCTGGGGTTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAt                                                                                 <  1:2543048/141‑1 (MQ=255)
                                                                        cgccgcgacgGACGAAAAATTATTCGTAAGGTTGGTTGTCTCCCTCTCCCTGTGGGATAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTg                                                                             <  2:3173227/145‑1 (MQ=255)
                                                                        cgacgcgacgGACTAAAAAGTATTCGTCCGGTTGGTTTTCGCCCTGTCCCTGTGGGAGCGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTg                                                                             <  2:2502471/148‑1 (MQ=255)
                                                                          acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCGAAACCAATCCCACAAGGGGGAGGGGAGCCCTAGTCCCCAAATTTTgag                                                                           >  2:2855812/1‑148 (MQ=21)
                                                                              gacggactAAAAATTATTCTTAAGGTGGGTTTTCCCCCTCCCCCGGTGGTAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat                                                                       <  2:1911364/148‑1 (MQ=38)
                                                                                cggacgAAAAGTTTTTCGTATGGTTGGTTTTCTCCCTCTCCCTGGGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat                                                                     <  2:2460614/148‑1 (MQ=255)
                                                                                 ggactAAAAATTATTGGTATGGTTGGTTTTCACCCTCTCCGTGTGGTAGAGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc                                                                    <  2:3692219/148‑1 (MQ=255)
                                                                                    cgAAAAAGTATCGTTAAGTTTGGTTTACCCCCACGCCGTGGGGTCGTGGGCGGGGGGGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc                                                                 <  1:2551437/148‑1 (MQ=255)
                                                                                      aaaatGTATTCGTACGGTGGGTTTTCGCCCTCCCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt                                                               <  2:2410524/148‑1 (MQ=38)
                                                                                      aaaaaGTATTCTTACGGTTGTTTTTCTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt                                                               <  2:2411364/148‑1 (MQ=255)
                                                                                      aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt                                                               <  2:490564/148‑1 (MQ=255)
                                                                                        aaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct                                                             <  2:3507042/148‑1 (MQ=255)
                                                                                         aagaagtCGTATGGTTGTTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATAGAGCACAAATTTTGAGAGATATCTTCTTCTg                                                            <  2:135853/142‑1 (MQ=38)
                                                                                              ttggTAGGGTTGGTTTCCGCCCTCGCGGGGGGGGTGTGGGCGGGGGTGGGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg                                                       <  2:2678222/145‑1 (MQ=255)
                                                                                                 ttatggttggttTTCCCCCCCTCCCTGGGGGAGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa                                                    <  2:1125788/144‑1 (MQ=255)
                                                                                                  tttggttggttTTCTCCCTCTCCGGGGGGGAGGGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAAc                                                   <  2:1802009/145‑1 (MQ=255)
                                                                                                  tctgtgtggttTCCCCCCTCCCCCGGGGGGGGCGGGCGGGGGGGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAAc                                                   <  2:809031/142‑1 (MQ=255)
                                                                                                     ggttggttTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga                                                <  2:3470303/148‑1 (MQ=255)
                                                                                                     ggttggtgTTCCCCCTCCCCTTGTGGGTGTGGGGCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga                                                <  2:722642/148‑1 (MQ=255)
                                                                                                      gttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                                               <  2:1874299/148‑1 (MQ=255)
                                                                                                      gtgggttTTCTCCCTCGCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagta                                               <  2:1874407/145‑3 (MQ=255)
                                                                                                      gtgggttTGCGCCCTCTCCGGGTGGTTGAGGGCGGGTGTGAGGGCCTCCGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCCCAAATTTTGAGAGATTTCTTCTTCTGTCTTGTAACagaa                                               <  2:2701810/145‑1 (MQ=255)
                                                                                                         ggttTTCGCCCTCGCCCTGGGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa                                            <  2:2510243/148‑1 (MQ=255)
                                                                                                         gggtttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAACAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa                                            <  2:2509093/145‑1 (MQ=255)
                                                                                                           ttttCCCCCCCGCCGTGGGGGCGCGGGGGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa                                          <  1:2991138/148‑1 (MQ=255)
                                                                                                              tccccccctccgtGGGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGaa                                       <  2:1245671/145‑1 (MQ=255)
                                                                                                                   ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCTCCCACACCCAAACCCGATCCCGCCAGGGGGAGGGGAGCGTTCGTGGACCAATTTTGTGTGTTTTATTCTTTTGTGGTGTTAAAGAATGACAGaaaaaag                                  >  2:2541404/1‑145 (MQ=12)
                                                                                                                   ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg                                  <  2:2082372/148‑1 (MQ=255)
                                                                                                                     cgccgtgtgGTCGAGGGCGGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg                                <  2:448349/143‑1 (MQ=255)
                                                                                                                       ccgggggggAGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCt                              <  2:2447755/142‑1 (MQ=255)
                                                                                                                           gtgggcgtgggcGGGGGGGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                          <  1:1092063/148‑1 (MQ=255)
                                                                                                                                  agGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGa                   <  2:1621870/148‑1 (MQ=255)
                                                                                                                                  agGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGa                   <  1:612093/148‑1 (MQ=255)
                                                                                                                                         gggggtgggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc            <  2:454901/142‑1 (MQ=255)
                                                                                                                                           gTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg          <  2:680354/148‑1 (MQ=255)
                                                                                                                                                 gCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa    <  1:2828895/148‑1 (MQ=255)
                                                                                                                                                   aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAg  <  2:406552/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAG  >  CP009273/160922‑161215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A