Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 161,068 A→C 23.5% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273161,0680AC23.5% 124.1 / 20.1 117intergenic (+47/‑149)hrpB/mrcBputative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (19/69);  new base C (7/22);  total (26/91)
Fisher's exact test for biased strand distribution p-value = 7.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

GTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA  >  CP009273/160921‑161217
                                                                                                                                                    |                                                                                                                                                     
gTGCGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCCCCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATc                                                                                                                                                     <  2:850459/150‑1 (MQ=255)
gTGAGGTGCAAAAAGAGATGAACGGGCGTTATCCCACACAGGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATc                                                                                                                                                     <  2:4631767/150‑1 (MQ=255)
  gAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAg                                                                                                                                                   >  2:1158257/1‑150 (MQ=255)
  gAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAg                                                                                                                                                   >  2:2520510/1‑150 (MQ=255)
    ggTTCAAAAAGTTATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTT‑TTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAgcg                                                                                                                                                 <  2:1444744/150‑1 (MQ=38)
       gCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcgc                                                                                                                                              >  2:3552684/1‑150 (MQ=255)
       gCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcgc                                                                                                                                              >  2:3552490/1‑150 (MQ=255)
          aaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACg                                                                                                                                           >  1:2504644/1‑150 (MQ=255)
           aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGt                                                                                                                                          >  1:1370075/1‑150 (MQ=255)
             atataTGAACGGGCGTTATCCCAAACTTGTCTGGCCGGACGACCCGGAAAATACGGCACCGACGCGACGGACGAAAAAGTA‑TTCTTATGTTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTtc                                                                                                                                        <  2:3973678/146‑1 (MQ=37)
             agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGtgc                                                                                                                                        >  2:3282262/1‑148 (MQ=255)
                taTGAATGGGCGTTATCCCAAAATTGTGTGGCGGGCCGACCCGGAAAATACTGCACCGACGCGACGGACTAAAATTTA‑TTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcacc                                                                                                                                     <  2:2587458/149‑1 (MQ=25)
                    aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggaccccca                                                                                                                                 >  2:2830759/1‑150 (MQ=255)
                        ggCTTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACTAAAAATTA‑TTCTTAAGGTTTGTTTTCTCCCTCGCCCTGGGGTTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTcaccctcaccct                                                                                                                             <  2:471079/150‑1 (MQ=38)
                         gCGTTATCCCAAACATGTCTGGCCGGACCACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTa                                                                                                                            <  2:1079391/150‑1 (MQ=255)
                           gTTATCCCAAACATGTTTGGCGGTACGACCCGTAAAATCCTGCACCGCCGCTCGGGACTAAAATTTT‑TGCTTATGGTTGTTTTTCTCCCTCTCCCTGTGGTTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc                                                                                                                          <  2:473193/150‑1 (MQ=19)
                           gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTGGGCCCCCACCCAAAc                                                                                                                          >  1:2561945/1‑150 (MQ=38)
                           gTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGTACTAAAAATTA‑TTCGTATGTTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc                                                                                                                          <  2:4404315/150‑1 (MQ=38)
                           gTTAACCCAAACTTTACTGGCGGCACGCCCCGCAAATTACTGCACGGAGGGGA‑GGCAGTAAAATTATTTCTTACGGTTGTTTTTCACCCTCTCCCTGTGGGATTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc                                                                                                                          <  2:473061/150‑1 (MQ=255)
                            ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCGCCCCCAAAcc                                                                                                                         >  1:3920737/1‑150 (MQ=38)
                                   aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGGCCCCGCCCCCAAACCCACAccc                                                                                                                  >  1:3289158/1‑150 (MQ=37)
                                    aaCATGTCTGGCCGTACGACCCGGCAAATACTTCACCCACGCGACGGACTAAAAAGTA‑TTCTTAAGTTTGTTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                                                 <  2:1488630/150‑1 (MQ=38)
                                    aaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGAACCGCACCCAAACCCACAcccc                                                                                                                 >  2:2424539/1‑149 (MQ=37)
                                         gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCACACCCAAACCCACACCCACaacg                                                                                                            >  2:612980/1‑148 (MQ=38)
                                         gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCACACCCAAAACCACACCCACAAgg                                                                                                            >  2:3751019/1‑150 (MQ=37)
                                          tCTGGCCGTACGACCCGGCAATTACTGCACCGACGCGACGGACGAAAAAGTA‑GTCGTAAGGTTGGTTTTCTCCCACTCCCTGTGGTAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAggg                                                                                                           <  2:1519331/150‑1 (MQ=255)
                                          tCTGGCCGGACGACCCGGCAAATACGGCACCGACGCGACGGACGAAAAAGTA‑TCCGTAAGGTTGGTTTTCCCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAggg                                                                                                           <  2:1519289/150‑1 (MQ=255)
                                          tCTGGCCGGACGACCCGCCAATTCCTGCACCCACGGTACGGACGAAAAAGTT‑TTCGTATGGTTGGTTTTCTCCCTCACCGTGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAggg                                                                                                           <  1:1148072/150‑1 (MQ=255)
                                           cgggCCGGACGACCCGGAAAATCCTGCACCGACGCGACGTACTAAAAAGTT‑TTCGTAAGGTTGGTTTTCCCCCTCTCCCTGTGGGTTCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga                                                                                                          <  1:2188124/148‑1 (MQ=255)
                                            tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTTGACCCGCCCCCAAAACCCCAACCAAAAGGgag                                                                                                         >  1:4214284/1‑150 (MQ=37)
                                            tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCGCACCCGAACCCACAACCGCAAgggcg                                                                                                         >  1:4214302/1‑148 (MQ=37)
                                             ggCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCGGTGGGAGAGGGCCTGGGTGAGGGCATCAGCGCGCACGTTCGCCCACAACCAAACCCACACCCACaacgtcag                                                                                                        >  2:3638127/1‑144 (MQ=37)
                                              gccggccgCCCCGGCAAATACTGCCCCGACGCGCGGGACTAAAATTTA‑TTCTTATGGTTGTTTTTCACCCCCTCCCGGGGGGTGTGGGGCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                                       <  1:705830/150‑1 (MQ=255)
                                               ccGGCCGCCCCGGCAAATCCTGCCCCGACGCGACGGACGAAAAATTA‑TTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGATAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg                                                                                                      <  2:2061323/150‑1 (MQ=38)
                                                    cgccccGGAAAATACTGCCCCGCCGCCACGGCCCAAAAATTA‑TTCTAAAGGTTGGTTTACGCCCCCTCCCTGTGGGAGAGGGCCGGGGGGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAcc                                                                                                 <  2:72752/147‑1 (MQ=255)
                                                    cgacCCGGAAAATACGGCACCGACGCGACGGACGAAAAATTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGCGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAcc                                                                                                 <  2:220236/150‑1 (MQ=38)
                                                     gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCTACTCCCTCAAGGGAGAGGGGACCg                                                                                                >  1:143209/1‑150 (MQ=255)
                                                     gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGGGCCCACCCCCCCACCCCCACCCACCAGGGGGCGGGGGCCg                                                                                                >  1:2907892/1‑150 (MQ=25)
                                                     gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCGCCCCCCAACACACAACCACAAGGGCGTGGGGGCCg                                                                                                >  1:1307791/1‑150 (MQ=25)
                                                      ccccGGAAAATCCGGCACCGACGCGACGGACGAAAAAGTA‑TTCGTACGGTTGTTTTTCCCCCTCTCCCTGTGGGAGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGa                                                                                               <  2:1830063/149‑1 (MQ=255)
                                                      acCCGGCAAACACGGCACCGCCGCGCCGGACGAAAAATTA‑TTCGAACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGa                                                                                               <  2:3923802/150‑1 (MQ=37)
                                                        ccGGCAAATACTGCCCCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc                                                                                             <  2:1036636/150‑1 (MQ=255)
                                                          ggCAAATACTGCACCGACGCGACGGACTAAAAAGTA‑TTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGATAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa                                                                                           <  2:3018745/150‑1 (MQ=255)
                                                            cAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCGCCCCCCAACCCACACCCGCCACGGAGAGGGGGCCGCTCGAGc                                                                                         >  2:508232/1‑150 (MQ=25)
                                                              aaTCCTGCACCGACGCGCCGGACTAAAACTTA‑TTCGTACGGTTGTTTTACCCCCTCGCCCTGGGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGcac                                                                                       <  1:3775166/150‑1 (MQ=255)
                                                               acacggCACCGACGCGAGGTACGAAAAAGTA‑TTCTTACGGTTGGTTTTCTCCCTCTCCCGGGGGGTGAGGGCCGGGGGGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGcaca                                                                                      <  2:667049/145‑1 (MQ=255)
                                                               aTACTTCCCTGACGCGACGGACTAAAAAGTT‑TTCGTAAGGTTGTTTTTGTCCCTCTCCCTGTGGTAGGGGGCCGGGTTTTGGGACTCCGCGCGCACGTTCTCCCTCACCCTAACCCTCTCCCTCAAGGGATAGGGGTACGATCGAGcaca                                                                                      <  2:667020/150‑1 (MQ=255)
                                                               aTACTGCCCCCACGCCACGGACCAAAAAGTA‑TTCGTAAGGTTGTTTTTCTCCCTAGCCCAGTGGGATAGGCCCGGGGTTAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGcaca                                                                                      <  2:2549459/150‑1 (MQ=255)
                                                               aTACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGcaca                                                                                      <  2:2601129/150‑1 (MQ=255)
                                                                   tGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCCCCCGCACCCAAACCCAAACCCACAAGGGGGAGGGGGCCGGTCGAGgacacaag                                                                                  >  2:884299/1‑146 (MQ=25)
                                                                    gccccGAGGCTAGGGACTAAAATTTT‑TTCTTATGTTTGGTTTTCGCCCCCCCCTTGGGGTATGGGGCGGGGTTGTGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAttt                                                                                 <  1:4131339/147‑1 (MQ=255)
                                                                    gccccGACGCGCCGGACTAAAATTTT‑TTGGTATGGTTGGTTTTCTCCCCCCCCGGGGGGGTGCGGGCGGGGGTGCGGCCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAttt                                                                                 <  1:4131457/147‑1 (MQ=255)
                                                                     ccccgccgCGACGGCCGAAAAATTA‑TGGTTATGGTTGGTTTTCGCCCACTCCCTGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt                                                                                <  1:956237/148‑1 (MQ=255)
                                                                         gacgcgcggGACTAAAATGTT‑TTCGTATGGTTGTTTTTCCCCCTCTCCCTGGGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga                                                                            <  1:2579948/150‑1 (MQ=255)
                                                                            gcgacgGACTAAAATGTT‑TTCGTATGGTTGGTTTTCGCCCCCGCCGTGTGGGTGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat                                                                         <  1:2799960/150‑1 (MQ=255)
                                                                              gacggactAAAAAGAT‑TTCGTAGGTTTGGTTTTCTCCATCTCCCTGTGGGATCGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat                                                                       <  1:4088766/150‑1 (MQ=255)
                                                                               ccggccgAAAACTTA‑TAGGTACGTTTGTTTTCCTCCCCCTCCGTGTGGTTGTGGGCGGGGTTTCGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc                                                                      <  2:774717/149‑1 (MQ=255)
                                                                                    cgAAATGGTA‑TTCGTTCGGGTTGTGTTTTCCGTGGCCCTGTGGGATTGGGCGGGGGTGAGGGCCTCAGCGCGCATGTTCACCCTCCCCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGTTCGAGCACAAATTTTGAGAGATAtcttctt                                                                 <  1:576112/150‑1 (MQ=255)
                                                                                     gAAAAAGTA‑TTCGTATGGTTGTTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                                                <  2:1476918/150‑1 (MQ=255)
                                                                                      aaaaaGTA‑TTCGTAAGGTTGGTTTTCCCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct                                                               <  2:1677868/150‑1 (MQ=255)
                                                                                       aaatttA‑TTCGTATGGTTGGTTTTCTCCCTCTCCCGGTGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTg                                                              <  1:2269479/145‑1 (MQ=255)
                                                                                           gTA‑TTCGTACGGTTGGTTTTCTCCCTCTCCCTGGGGGAGCGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                          <  1:3063444/150‑1 (MQ=255)
                                                                                           gTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                          <  2:668001/150‑1 (MQ=255)
                                                                                           gTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                          <  2:1020305/150‑1 (MQ=255)
                                                                                           gTA‑TTCGTAAGGTTGGTTTACTCCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                          <  2:668020/150‑1 (MQ=255)
                                                                                            gttttCTTATGGTTGTTTTTCTCCCTCTCCCTGGGGGATCGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                          <  1:3063446/147‑1 (MQ=38)
                                                                                                    acgtttgtttTTCCCCCACCCCCTGGGGTTTTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga                                                  <  2:24540/146‑1 (MQ=255)
                                                                                                       tttgtttTTCCCCCTCACCTTGTGGTTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga                                               <  1:259336/149‑1 (MQ=255)
                                                                                                           gttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCCCACCCAAAACCACTCCCACCACGGGGAGGGGGCCGATCGGGCACAAATTTTGTGCTTTATATTGTTAAGTGTTGTAAAAGAATAACac                                           >  2:2964286/1‑149 (MQ=12)
                                                                                                              ttCCCCCTCTCCGTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATTTTCTTCTGTCTTGTAACAGAAGAACAGaaa                                        <  1:4286739/150‑1 (MQ=255)
                                                                                                                  ccctccccctGTGGTTTTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg                                    <  2:1169401/150‑1 (MQ=255)
                                                                                                                    ctctccctGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg                                  <  2:3406521/150‑1 (MQ=255)
                                                                                                                     tctccctGTGGTAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGc                                 <  1:287887/150‑1 (MQ=255)
                                                                                                                     tccccgtgtgGGAGTGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGc                                 <  1:288003/144‑1 (MQ=255)
                                                                                                                      ccccgtgtgGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCt                                <  1:4072410/145‑1 (MQ=255)
                                                                                                                       tccctGGGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtt                               <  1:1064196/150‑1 (MQ=255)
                                                                                                                        ccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGAGATCTTCTTCTGTCTTGTAACAGAAGAACCGAAAATCGGGCttt                              <  2:2918812/150‑1 (MQ=255)
                                                                                                                        ccctGGGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACACGAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt                              <  2:2918802/150‑1 (MQ=255)
                                                                                                                         ccggtgGGAGCGGGCGGGGTTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtttt                             <  2:1932658/147‑1 (MQ=255)
                                                                                                                          ctGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                            <  1:3402903/150‑1 (MQ=255)
                                                                                                                          cggggggAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                            <  2:3735575/146‑1 (MQ=255)
                                                                                                                            gtgGGAGTGGGCGGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcg                          <  2:794348/150‑1 (MQ=255)
                                                                                                                             tgGTAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc                         <  2:4341993/150‑1 (MQ=255)
                                                                                                                             tgGGAGAGGGCCGGGGGGCGGGCCTCAGCGCGCACCTTCACCCTCACCCTACCCCTCCCCCTCAAGGGAGAGGGCACCGATCGAGCACAAATTTTGAGAGATATCTTCGTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc                         <  2:4342524/150‑1 (MQ=255)
                                                                                                                                gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCGAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAACTTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAACCTCGGGCTTTTGCGCCTg                      <  2:1113948/150‑1 (MQ=255)
                                                                                                                                    gggCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAata                  <  2:2131095/150‑1 (MQ=255)
                                                                                                                                    gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAata                  <  2:3659366/150‑1 (MQ=255)
                                                                                                                                      gCCGGTGTGAGTGCATCAGCGCGCACGTTCACCCTCTCACTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATAtt                <  1:4160974/150‑1 (MQ=255)
                                                                                                                                      gCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATAtt                <  2:4088711/150‑1 (MQ=255)
                                                                                                                                        gggggTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc              <  1:2132113/149‑1 (MQ=255)
                                                                                                                                          gggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg            <  1:3564089/150‑1 (MQ=255)
                                                                                                                                          gggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACATAAAATCGGGCTTTTGCGCCTGAATATTGCgg            <  2:1064192/150‑1 (MQ=255)
                                                                                                                                           ggTGTGGGCATCAGCGCGCACGTTCACCATCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGTTATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGTGga           <  2:3648713/150‑1 (MQ=255)
                                                                                                                                            gTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGgag          <  2:4503499/150‑1 (MQ=255)
                                                                                                                                             tgtgggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGgaga         <  2:1147464/147‑1 (MQ=255)
                                                                                                                                              gcgggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaa        <  2:2807302/148‑1 (MQ=255)
                                                                                                                                               aGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaa       <  1:4101321/150‑1 (MQ=255)
                                                                                                                                                gggCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa      <  1:1236660/150‑1 (MQ=255)
                                                                                                                                                gggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa      <  1:3112617/150‑1 (MQ=255)
                                                                                                                                                    aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAATTTGAGAGATATCTTCTTCGGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCa  <  2:2510604/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTA‑TTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA  >  CP009273/160921‑161217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A