breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 60 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,612,0670AG58.8% 1.6 / 15.1 17E161G (GAG→GGG) yjjJtype II toxin‑antitoxin system HipA family toxin YjjJ
*NZ_CP0092731,617,0720CA54.5% 0.4 / 13.1 11G25G (GGC→GGAmgtTprotein MgtT
*NZ_CP0092731,617,0730GA54.5% ‑4.5 / 11.8 11E26K (GAA→AAA) mgtTprotein MgtT
*NZ_CP0092731,608,1610AC53.3% 13.6 / 20.4 15V267G (GTA→GGA) sadsuccinate‑semialdehyde dehydrogenase
*NZ_CP009273497,0130AC52.4% 18.0 / 28.3 21intergenic (+128/+5)gsk/ybaLinosine/guanosine kinase/Kef family K(+) transporter
*NZ_CP00927371,5660CT50.0% 4.3 / 11.3 16L134L (CTG→CTAthiBthiamine ABC transporter substrate binding subunit
*NZ_CP0092733,199,7940CT48.4% 11.8 / 20.6 31intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
*NZ_CP0092733,634,3410CT47.8% 11.9 / 11.2 23F40F (TTC→TTTdtpBdipeptide/tripeptide permease DtpB
*NZ_CP0092731,453,6040CA47.1% 12.2 / 13.3 17V98V (GTC→GTApaaC3‑hydroxyacyl‑CoA dehydrogenase PaaC
*NZ_CP009273324,5180AT45.7% 50.4 / 51.0 72D176E (GAT→GAAbetItranscriptional regulator BetI
*NZ_CP0092732,380,3410GA45.0% 14.6 / 12.3 20intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP009273353,6010CA43.5% 38.2 / 41.7 63L267F (TTG→TTTcynRtranscriptional regulator CynR
*NZ_CP0092733,502,0160AG43.3% 40.3 / 10.2 30F386S (TTC→TCC) yhfWphosphopentomutase
*NZ_CP009273353,5910TC42.1% 106.4 / 27.1 76K271E (AAA→GAA) cynRtranscriptional regulator CynR
*NZ_CP0092733,611,7230GA41.7% 22.9 / 11.2 24K196K (AAG→AAAnikEnickel import ATP‑binding protein NikE
*NZ_CP0092734,594,1610CT40.9% 45.7 / 20.4 45S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP009273324,5140TG40.8% 99.2 / 44.1 71T178P (ACC→CCC) betItranscriptional regulator BetI
*NZ_CP009273324,5020AG40.3% 103.3 / 27.6 72S182P (TCC→CCC) betItranscriptional regulator BetI
*NZ_CP0092733,012,9510AT39.3% 36.1 / 18.6 28L144F (TTA→TTTssnAputative aminohydrolase SsnA
*NZ_CP0092734,594,1670CT37.8% 47.4 / 20.6 45S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 314763 =127 (3.710)17 (0.500) 14/248 0.8 11.8% coding (950/1326 nt) rclA reactive chlorine resistance oxidoreductase RclA
?NZ_CP009273 376716 = NA (NA)coding (28/444 nt) BW25113_RS01855 transferase
* ? NZ_CP009273 4532499 =13 (0.380)5 (0.160) 5/230 1.9 19.2% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 30 (0.950)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 = 28358383 (2.420)4 (0.120) 4/234 2.1 4.1% coding (1084/1611 nt) yagH glycoside hydrolase family 43 protein
?NZ_CP009273 = 283644 109 (3.380)coding (1145/1611 nt) yagH glycoside hydrolase family 43 protein
* ? NZ_CP009273 1203246 =19 (0.550)3 (0.100) 3/216 2.3 17.4% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 12 (0.400)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 3342514 =38 (1.110)3 (0.090) 3/234 2.4 8.0% coding (655/729 nt) mtgA monofunctional biosynthetic peptidoglycan transglycosylase
?NZ_CP009273 3342530 = 33 (1.020)coding (639/729 nt) mtgA monofunctional biosynthetic peptidoglycan transglycosylase
* ? NZ_CP009273 2930783 =52 (1.520)3 (0.100) 3/226 2.4 6.5% intergenic (+65/‑44) fucI/fucK L‑fucose isomerase/L‑fuculokinase
?NZ_CP009273 2930811 = 39 (1.250)intergenic (+93/‑16) fucI/fucK L‑fucose isomerase/L‑fuculokinase