breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 40 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_CP034943 | 3,285,647 | 0 | C | A | 44.9% | 114.4 / 268.6 | 196 | V252L (GTA→TTA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 3,285,580 | 0 | A | G | 42.4% | 123.8 / 238.9 | 170 | I274T (ATA→ACA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 1,730,075 | 0 | G | C | 41.8% | 47.8 / 20.3 | 58 | E166Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
* | NZ_CP034943 | 3,285,713 | 0 | G | A | 39.0% | 109.9 / 114.2 | 97 | L230L (CTA→TTA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 3,285,575 | 0 | T | G | 38.8% | 178.7 / 203.6 | 178 | N276H (AAT→CAT) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 3,285,567 | 0 | C | T | 37.6% | 188.8 / 214.6 | 178 | L278L (CTG→CTA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 3,446,970 | 0 | G | T | 32.3% | 178.0 / 39.5 | 131 | Q206K (CAA→AAA) | degU | two‑component system response regulator DegU |
* | NZ_CP034943 | 2,270,064 | 0 | G | C | 32.1% | 131.2 / 16.0 | 87 | L39V (CTA→GTA) | prsW | glutamic‑type intramembrane protease PrsW |
* | NZ_CP034943 | 2,270,052 | 0 | G | C | 30.6% | 138.0 / 17.1 | 89 | L43V (CTT→GTT) | prsW | glutamic‑type intramembrane protease PrsW |
* | NZ_CP034943 | 1,730,096 | 0 | G | C | 28.8% | 67.6 / 14.1 | 60 | E173Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
* | NZ_CP034943 | 177,051 | 0 | T | A | 28.1% | 153.2 / 24.8 | 83 | intergenic (+224/‑48) | EO946_RS01020/EO946_RS01025 | alcohol dehydrogenase/SulP family inorganic anion transporter |
* | NZ_CP034943 | 1,111,649 | 0 | C | A | 27.9% | 233.1 / 13.4 | 111 | P582T (CCG→ACG) | addB | helicase‑exonuclease AddAB subunit AddB |
* | NZ_CP034943 | 2,991,236 | 0 | C | G | 27.5% | 180.2 / 21.6 | 94 | intergenic (+37/‑170) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
* | NZ_CP034943 | 17,887 | 0 | G | T | 26.6% | 236.3 / 22.1 | 126 | E248D (GAG→GAT) | dacA | D‑alanyl‑D‑alanine carboxypeptidase |
* | NZ_CP034943 | 3,539,592 | 0 | C | G | 26.6% | 253.5 / 22.5 | 131 | E121Q (GAA→CAA) | EO946_RS18215 | T7SS effector LXG polymorphic toxin |
* | NZ_CP034943 | 240,198 | 0 | G | T | 26.3% | 262.3 / 28.8 | 157 | Q112K (CAA→AAA) | EO946_RS01360 | hypothetical protein |
* | NZ_CP034943 | 1,512,617 | 0 | G | T | 24.7% | 166.8 / 11.8 | 81 | A11A (GCG→GCT) | typA | translational GTPase TypA |
* | NZ_CP034943 | 177,056 | 0 | C | A | 24.4% | 135.1 / 14.1 | 78 | intergenic (+229/‑43) | EO946_RS01020/EO946_RS01025 | alcohol dehydrogenase/SulP family inorganic anion transporter |
* | NZ_CP034943 | 3,394,367 | 0 | C | G | 24.3% | 368.0 / 19.7 | 157 | G178A (GGA→GCA) | ppaX | pyrophosphatase PpaX |
* | NZ_CP034943 | 595,186 | 0 | G | T | 23.4% | 253.0 / 17.5 | 128 | L24F (TTG→TTT) | EO946_RS03130 | MMPL family transporter |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP034943 | = 607279 | 159 (1.230) | 7 (0.130) +79 bp |
4/114 | 3.7 | 10.2% | coding (122/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607323 = | 138 (1.070) | coding (166/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | 607566 = | 100 (0.770) | 25 (0.210) +CGCTCCGGT |
10/254 | 4.8 | 22.3% | coding (409/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | = 607637 | 87 (0.670) | coding (480/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607210 | 184 (1.420) | 8 (0.060) | 5/268 | 6.9 | 5.1% | coding (53/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607526 = | 119 (0.930) | coding (369/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607210 | 184 (1.420) | 8 (0.060) | 5/268 | 6.9 | 5.1% | coding (53/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607427 = | 115 (0.900) | coding (270/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607210 | 185 (1.430) | 4 (0.030) +AGCGACA |
4/258 | 7.3 | 2.4% | coding (53/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607281 = | 161 (1.240) | coding (124/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607210 | 184 (1.420) | 5 (0.040) | 4/268 | 7.5 | 3.1% | coding (53/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607481 = | 127 (1.000) | coding (324/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607225 | 169 (1.310) | 3 (0.030) +AGGGGCTACT |
3/252 | 7.7 | 2.6% | coding (68/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607623 = | 75 (0.580) | coding (466/1125 nt) | EO946_RS03185 | collagen‑like protein |