breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 40 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,285,6470CA44.9% 114.4 / 268.6 196V252L (GTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5800AG42.4% 123.8 / 238.9 170I274T (ATA→ACA) EO946_RS16995IS3 family transposase
*NZ_CP0349431,730,0750GC41.8% 47.8 / 20.3 58E166Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP0349433,285,7130GA39.0% 109.9 / 114.2 97L230L (CTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5750TG38.8% 178.7 / 203.6 178N276H (AAT→CAT) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5670CT37.6% 188.8 / 214.6 178L278L (CTG→CTAEO946_RS16995IS3 family transposase
*NZ_CP0349433,446,9700GT32.3% 178.0 / 39.5 131Q206K (CAA→AAA) degUtwo‑component system response regulator DegU
*NZ_CP0349432,270,0640GC32.1% 131.2 / 16.0 87L39V (CTA→GTA) prsWglutamic‑type intramembrane protease PrsW
*NZ_CP0349432,270,0520GC30.6% 138.0 / 17.1 89L43V (CTT→GTT) prsWglutamic‑type intramembrane protease PrsW
*NZ_CP0349431,730,0960GC28.8% 67.6 / 14.1 60E173Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP034943177,0510TA28.1% 153.2 / 24.8 83intergenic (+224/‑48)EO946_RS01020/EO946_RS01025alcohol dehydrogenase/SulP family inorganic anion transporter
*NZ_CP0349431,111,6490CA27.9% 233.1 / 13.4 111P582T (CCG→ACG) addBhelicase‑exonuclease AddAB subunit AddB
*NZ_CP0349432,991,2360CG27.5% 180.2 / 21.6 94intergenic (+37/‑170)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP03494317,8870GT26.6% 236.3 / 22.1 126E248D (GAG→GATdacAD‑alanyl‑D‑alanine carboxypeptidase
*NZ_CP0349433,539,5920CG26.6% 253.5 / 22.5 131E121Q (GAA→CAA) EO946_RS18215T7SS effector LXG polymorphic toxin
*NZ_CP034943240,1980GT26.3% 262.3 / 28.8 157Q112K (CAA→AAA) EO946_RS01360hypothetical protein
*NZ_CP0349431,512,6170GT24.7% 166.8 / 11.8 81A11A (GCG→GCTtypAtranslational GTPase TypA
*NZ_CP034943177,0560CA24.4% 135.1 / 14.1 78intergenic (+229/‑43)EO946_RS01020/EO946_RS01025alcohol dehydrogenase/SulP family inorganic anion transporter
*NZ_CP0349433,394,3670CG24.3% 368.0 / 19.7 157G178A (GGA→GCA) ppaXpyrophosphatase PpaX
*NZ_CP034943595,1860GT23.4% 253.0 / 17.5 128L24F (TTG→TTTEO946_RS03130MMPL family transporter

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 607279159 (1.230)7 (0.130)
+79 bp
4/114 3.7 10.2% coding (122/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607323 = 138 (1.070)coding (166/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607566 =100 (0.770)25 (0.210)
+CGCTCCGGT
10/254 4.8 22.3% coding (409/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607637 87 (0.670)coding (480/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607210184 (1.420)8 (0.060) 5/268 6.9 5.1% coding (53/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607526 = 119 (0.930)coding (369/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607210184 (1.420)8 (0.060) 5/268 6.9 5.1% coding (53/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607427 = 115 (0.900)coding (270/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607210185 (1.430)4 (0.030)
+AGCGACA
4/258 7.3 2.4% coding (53/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607281 = 161 (1.240)coding (124/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607210184 (1.420)5 (0.040) 4/268 7.5 3.1% coding (53/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607481 = 127 (1.000)coding (324/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607225169 (1.310)3 (0.030)
+AGGGGCTACT
3/252 7.7 2.6% coding (68/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607623 = 75 (0.580)coding (466/1125 nt) EO946_RS03185 collagen‑like protein