breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 151 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,958,2581.C70.7% 347.2 / 163.2 157intergenic (‑827/‑34)ppiC/reppeptidyl‑prolyl cis‑trans isomerase C/rep helicase
*NC_0009133,941,0900TC70.2% 82.0 / 98.9 121noncoding (34/76 nt)gltUtRNA‑Val
*NC_0009131,634,0190TC65.2% 21.5 / 17.9 23I125V (ATT→GTT) nohANohA
*NC_000913580,4980GA61.7% 35.4 / 41.8 47V148I (GTT→ATT) nohBbacteriophage DNA packaging protein
*NC_0009131,096,5790TC60.0% 13.3 / 10.1 15intergenic (+527/+209)ycdU/serXhypothetical protein/tRNA‑Ser
*NC_0009133,620,2190AG60.0% 50.9 / 93.2 85M1133V (ATG→GTG) rhsBRhsB
*NC_000913580,4250TA56.4% 50.8 / 53.4 39G123G (GGT→GGAnohBbacteriophage DNA packaging protein
*NC_000913580,4190CG55.7% 77.0 / 91.3 61I121M (ATC→ATGnohBbacteriophage DNA packaging protein
*NC_000913580,5090GC55.2% 105.3 / 149.8 96L151L (CTG→CTCnohBbacteriophage DNA packaging protein
*NC_000913580,5120AG55.1% 88.3 / 126.7 98K152K (AAA→AAGnohBbacteriophage DNA packaging protein
*NC_0009131,634,0230AT53.6% 35.1 / 39.9 28G123G (GGT→GGAnohANohA
*NC_0009133,192,8730TC53.3% ‑3.9 / inf 413intergenic (+332/+88)b3051/b3052putative membrane protein/putative kinase
*NC_000913580,4220AC52.9% 71.5 / 75.5 51A122A (GCA→GCCnohBbacteriophage DNA packaging protein
*NC_0009133,620,3210TC52.4% 84.5 / inf 453C1167R (TGC→CGC) rhsBRhsB
*NC_0009133,192,8910CT52.1% 145.4 / inf 396intergenic (+350/+70)b3051/b3052putative membrane protein/putative kinase
*NC_0009131,949,3560TC51.9% 9.9 / inf 412S178S (AGT→AGCyecDhypothetical protein
*NC_0009133,192,9070AT51.4% 35.6 / inf 382intergenic (+366/+54)b3051/b3052putative membrane protein/putative kinase
*NC_0009131,949,3590CT51.3% 123.5 / inf 407I179I (ATC→ATTyecDhypothetical protein
*NC_0009132,460,9002.C50.5% 149.6 / 178.2 93intergenic (+234/‑132)fadL/b2345FadL/hypothetical protein
*NC_0009131,949,3620CT50.5% 88.4 / inf 406N180N (AAC→AATyecDhypothetical protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 40993023 (0.010)119 (0.500)
+GGG
65/164 5.3 97.5% coding (41/993 nt) kdgT 2‑keto‑3‑deoxy‑D‑gluconate transport system
?NC_000913 4099303 = 3 (0.010)coding (42/993 nt) kdgT 2‑keto‑3‑deoxy‑D‑gluconate transport system
* ? NC_000913 = 246089871 (0.290)75 (0.320)
+CCC
50/176 9.8 52.1% intergenic (+232/‑134) fadL/b2345 FadL/hypothetical protein
?NC_000913 2460899 = 71 (0.290)intergenic (+233/‑133) fadL/b2345 FadL/hypothetical protein
* ? NC_000913 = 400839876 (0.320)59 (0.250)
+AAGA
22/172 19.2 44.5% coding (481/516 nt) b4555 hypothetical protein
?NC_000913 4008399 = 76 (0.310)coding (482/516 nt) b4555 hypothetical protein
* ? NC_000913 1832834 =216 (0.880)3 (0.010) 3/164 31.4 1.5% coding (3/708 nt) ydjZ hypothetical protein
?NC_000913 = 1832830 193 (0.880)intergenic (+13/‑2) ydjY/ydjZ hypothetical protein/hypothetical protein