breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
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evidence | position | mutation | annotation | gene | description |
RA | 473,924 | A→C | V461G (GTC→GGC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
RA | 795,321 | (G)6→7 | intergenic (+26/+16) | ECB_00736 → / ← ECB_00737 | hypothetical protein/hypothetical protein |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
JC JC | 1,941,961 | IS186 (–) +8 bp :: Δ2 bp | coding (1853‑1860/2079 nt) | flhA ← | flagellar biosynthesis protein A |
MC JC | 2,100,308 | Δ22,146 bp | ogrK–[ECB_02013] | 27 genes ogrK, yegZ, ECB_01989, ECB_01990, ECB_01991, ECB_01992, ECB_01993, ECB_01994, ECB_01995, ECB_01996, ECB_01997, ECB_01998, ECB_01999, ECB_02000, ECB_02001, ECB_02002, ECB_02003, ECB_02004, ECB_02005, ECB_02006, ECB_02007, ECB_02008, ECB_02009, ECB_02010, ECB_02011, ECB_02012, [ECB_02013] |
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RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 2,562,715 | G→A | R317C (CGT→TGT) | yfgA ← | hypothetical protein |
JC JC | 3,015,771 | IS150 (–) +3 bp | coding (1192‑1194/1677 nt) | ECB_02816 → | KpsD protein |
JC | 3,339,054 | Δ5 bp | coding (884‑888/966 nt) | yhdG → | tRNA‑dihydrouridine synthase B |
RA | 3,488,896 | T→G | E104A (GAA→GCA) | glpR ← | DNA‑binding transcriptional repressor |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 3,911,972 | (T)7→6 | intergenic (‑173/‑150) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
RA | 4,163,089 | A→T | E745V (GAA→GTA) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,164,845 | T→G | I1330M (ATT→ATG) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,262,921 | C→T | M305I (ATG→ATA) | actP ← | acetate permease |
RA | 4,275,544 | T→C | D135G (GAT→GGT) | yjcO ← | hypothetical protein |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 546984–547700 | 593405 | 45706–46422 | 19 [18] | [18] 19 | [insB‑6]–[fepA] | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
* | * | ÷ | REL606 | 593573 | 619850–619116 | 25544–26278 | 20 [17] | [18] 21 | [fepA]–insA‑8 | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8 |