breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
JC JC | 294,446 | IS1 (+) +9 bp | coding (190‑198/720 nt) | ykgE → | predicted oxidoreductase |
RA | 465,735 | A→C | Q526P (CAG→CCG) | dnaX → | DNA polymerase III subunits gamma and tau |
RA | 473,924 | A→C | V461G (GTC→GGC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
RA | 648,692 | C→T | R69H (CGC→CAC) | mrdB ← | cell wall shape‑determining protein |
RA | 825,980 | G→A | D96N (GAT→AAT) | dinG → | ATP‑dependent DNA helicase |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 2,058,079 | C→A | P291P (CCG→CCT) | wcaI ← | predicted glycosyl transferase |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
MC JC | 2,298,759 | Δ4 bp | coding (321‑324/1359 nt) | glpT ← | sn‑glycerol‑3‑phosphate transporter |
RA | 3,028,373 | A→T | L30Q (CTG→CAG) | ECB_02828 ← | Polysialic acid transport protein kpsM |
RA | 3,145,853 | G→A | intergenic (+184/‑11) | dnaG → / → rpoD | DNA primase/RNA polymerase sigma factor |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 4,162,569 | A→C | I572L (ATC→CTC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,202,426 | G→A | A45V (GCA→GTA) | iclR ← | DNA‑binding transcriptional repressor |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 2035140 | NA (NA) | 7 (0.350) | 4/134 | 2.2 | 20.0% | coding (717/768 nt) | wbbD | putative glycosyltransferase WbbD |
? | REL606 | = 2035556 | 28 (1.420) | coding (301/768 nt) | wbbD | putative glycosyltransferase WbbD | |||||
* | ? | REL606 | 2038432 = | 23 (1.040) | 9 (0.460) | 4/134 | 2.2 | 24.2% | coding (517/822 nt) | wbbB | putative glycosyltransferase WbbB |
? | REL606 | 2038941 = | 36 (1.820) | coding (8/822 nt) | wbbB | putative glycosyltransferase WbbB |