breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein
RA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,312,590 G→A Q54* (CAG→TAG)  ompC ← outer membrane porin protein C
RA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 3,413,571 T→C intergenic (‑106/‑293) acrS ← / → acrE acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,114,419 G→A A18A (GCC→GCT fpr ← ferredoxin‑NADP reductase; flavodoxin reductase
RA 4,114,456 G→A T6I (ACA→ATA)  fpr ← ferredoxin‑NADP reductase; flavodoxin reductase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 583319 583338 20 4 [1] [0] 2 tfaX/appY pseudogene, DLP12 prophage;Phage or Prophage Related/global transcriptional activator; DLP12 prophage
* * ÷ NC_000913 583521 583541 21 2 [1] [1] 2 tfaX/appY pseudogene, DLP12 prophage;Phage or Prophage Related/global transcriptional activator; DLP12 prophage
* * ÷ NC_000913 777121 777128 8 2 [0] [0] 2 tolA membrane anchored protein in TolA‑TolQ‑TolR complex
* * ÷ NC_000913 1012464 1012478 15 2 [1] [1] 2 pqiA paraquat‑inducible, SoxRS‑regulated inner membrane protein
* * ÷ NC_000913 1024871 1024883 13 2 [1] [1] 2 helD DNA helicase IV
* * ÷ NC_000913 1128415 1128442 28 2 [1] [1] 2 murJ putative lipid II flippase
* * ÷ NC_000913 1191811 1191829 19 2 [1] [1] 2 purB adenylosuccinate lyase
* * ÷ NC_000913 1530073 1530096 24 2 [1] [1] 2 ydcD putative immunity protein for RhsE
* * ÷ NC_000913 2470238 2470266 29 2 [1] [1] 2 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ NC_000913 2774393 2774402 10 2 [1] [1] 2 yfjW CP4‑57 prophage; putative inner membrane protein
* * ÷ NC_000913 3360924 3360944 21 2 [1] [1] 4 gltD/gltF glutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein
* * ÷ NC_000913 3423718–3424234 3424589–3424238 5–872 2 [0] [1] 4 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3769789 3769827 39 2 [0] [1] 2 yibV/yibU pseudogene, putative cryptic Rhs immunity protein/pseudogene, H repeat‑associated protein
* * ÷ NC_000913 3847509 3847526 18 2 [1] [0] 3 uhpC membrane protein regulates uhpT expression
* * ÷ NC_000913 3887015 3887023 9 2 [1] [1] 2 mnmE tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
* * ÷ NC_000913 3906796 3906808 13 3 [1] [0] 3 bglG/phoU transcriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator
* * ÷ NC_000913 4008297 4008311 15 3 [1] [1] 4 rhtC threonine efflux pump
* * ÷ NC_000913 4296098–4296380 4296387 8–290 9 [1] [1] 2 gltP/yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
* * ÷ NC_000913 4326926 4326937 12 2 [1] [1] 2 yjdM/crfC zinc‑ribbon family protein/clamp‑binding sister replication fork colocalization protein, dynamin‑related

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2356678 (0.420)3 (0.190) 3/112 1.5 35.8% coding (336/468 nt) rnhA ribonuclease HI, degrades RNA of DNA‑RNA hybrids
?NC_000913 = 235673 4 (0.250)coding (330/468 nt) rnhA ribonuclease HI, degrades RNA of DNA‑RNA hybrids
* ? NC_000913 969360 =10 (0.530)3 (0.180) 3/114 1.5 27.8% intergenic (+8/‑29) lpxK/ycaQ lipid A 4'kinase/DUF1006 family protein with C‑terminal wHTH domain
?NC_000913 969402 = 7 (0.430)coding (14/1233 nt) ycaQ DUF1006 family protein with C‑terminal wHTH domain
* ? NC_000913 1207790 =6 (0.320)13 (0.910) 11/100 0.2 77.6% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 3 (0.210)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078056 (0.320)8 (0.560) 5/100 0.8 68.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 3 (0.210)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 3619916 =NA (NA)3 (0.170) 3/126 1.6 NA coding (725/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 3619969 = NA (NA)coding (778/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor