breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 75,042 T→C 3.8% S147G (AGC→GGC)  thiB ← thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
RA 91,112 Δ1 bp 2.9% coding (81/366 nt) ftsL → membrane bound cell division leucine zipper septum protein
RA 188,371 C→T 2.5% G94S (GGC→AGC)  glnD ← uridylyltransferase
RA 285,198 Δ1 bp 3.1% intergenic (+30/‑197) yagF → / → yagG CP4‑6 prophage, dehydratase family protein/CP4‑6 prophage, putative sugar transporter
RA 285,199 Δ1 bp 3.1% intergenic (+31/‑196) yagF → / → yagG CP4‑6 prophage, dehydratase family protein/CP4‑6 prophage, putative sugar transporter
RA 325,458 Δ1 bp 2.6% intergenic (‑94/+119) ykgH ← / ← betA putative inner membrane protein/choline dehydrogenase, a flavoprotein
RA 377,431:1 +A 2.7% intergenic (+120/+104) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 377,432 C→G 3.1% intergenic (+121/+103) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 377,451 T→A 2.5% intergenic (+140/+84) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 509,839 G→A 3.5% A514V (GCC→GTC)  copA ← copper transporter
RA 509,844 T→C 3.5% Q512Q (CAA→CAG copA ← copper transporter
JC 700,783 Δ9 bp 9.4% coding (804‑812/1221 nt) nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
RA 701,378:1 +T 4.1% coding (217/1221 nt) nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
RA 702,331 Δ1 bp 8.1% coding (421/1149 nt) nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 982,776 T→A 2.7% L577Q (CTG→CAG)  ldtD → murein L,D‑transpeptidase
RA 1,034,228 G→T 2.8% A325A (GCG→GCT hyaB → hydrogenase 1, large subunit
RA 1,158,171 Δ1 bp 9.2% coding (303/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
RA 1,158,383 G→A 14.2% W172* (TGG→TAG)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
JC 1,159,286:1 +GTA 7.8% coding (1418/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
RA 1,240,971 G→C 4.2% V367V (GTC→GTG cvrA ← putative cation/proton antiporter
RA 1,269,785 G→A 3.4% T9I (ACT→ATT)  ldrB ← toxic polypeptide, small
RA 1,464,842 A→G 2.6% K124K (AAA→AAG paaY → thioesterase required for phenylacetic acid degradation, trimeric, phenylacetate regulatory and detoxification protein, hexapeptide repeat protein
RA 1,466,201 T→C 4.0% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,617,802:1 +A 2.7% coding (775/1191 nt) ydeA → arabinose efflux transporter, arabinose‑inducible
RA 1,708,662 C→G 5.8% Q582E (CAA→GAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,718 G→A 10.2% A600A (GCG→GCA rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,748 G→C 7.3% A610A (GCG→GCC rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,751 A→G 9.7% E611E (GAA→GAG rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,930 C→A 6.7% P671Q (CCG→CAG)  rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,973 T→C 5.8% A685A (GCT→GCC rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,745,063 Δ1 bp 2.5% coding (1065/1257 nt) ydhQ ← autotransporter adhesin‑related protein
RA 1,947,040 Δ1 bp 2.6% coding (337/522 nt) ruvC ← component of RuvABC resolvasome, endonuclease
RA 1,964,263:1 +G 2.8% coding (788/2079 nt) flhA ← putative flagellar export pore protein
RA 2,012,757 A→T 3.8% I86I (ATT→ATA fliE ← flagellar basal‑body component
RA 2,131,514 T→C 6.8% E186G (GAG→GGG)  wcaC ← putative glycosyl transferase
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,297,368 G→A 3.2% T92M (ACG→ATG)  ccmA ← heme export ABC transporter ATPase
RA 2,304,678 T→C 9.4% intergenic (+287/+428) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,314,714 C→A 3.8% A410E (GCG→GAG)  rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
RA 2,368,244 C→T 3.0% R70C (CGC→TGC)  arnA → fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase
RA 2,375,995 Δ1 bp 2.6% coding (823/858 nt) menB ← dihydroxynaphthoic acid synthetase
RA 2,375,996 Δ1 bp 2.6% coding (822/858 nt) menB ← dihydroxynaphthoic acid synthetase
RA 2,375,997 Δ1 bp 2.6% coding (821/858 nt) menB ← dihydroxynaphthoic acid synthetase
RA 2,477,020 T→G 2.6% D203A (GAC→GCC)  dsdC ← dsd operon activator, autorepressor
RA 2,528,973 T→C 5.4% E401G (GAG→GGG)  ligA ← DNA ligase, NAD(+)‑dependent
RA 2,566,735 T→C 3.8% S50G (AGT→GGT)  eutA ← reactivating factor for ethanolamine ammonia lyase
RA 2,619,724 A→C 9.9% G145G (GGT→GGG uraA ← uracil permease
RA 2,942,491 G→A 2.8% R25R (CGC→CGT gcvA ← glycine cleavage system transcriptional activator, autorepressor
RA 3,088,199 C→A 8.6% intergenic (+341/‑83) metK → / → galP S‑adenosylmethionine synthetase/D‑galactose transporter
RA 3,127,226 T→C 5.2% E265G (GAG→GGG)  glcD ← glycolate oxidase subunit, FAD‑linked
RA 3,233,187 G→T 9.6% D509Y (GAT→TAT)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
RA 3,271,939 C→A 2.8% G271C (GGT→TGT)  garR ← tartronate semialdehyde reductase
RA 3,320,743 T→A 2.6% H290L (CAT→CTT)  yhbX ← putative EptAB family phosphoethanolamine transferase, inner membrane protein
RA 3,381,847 A→C 2.8% E369D (GAA→GAC degQ → serine endoprotease, periplasmic
RA 3,495,036 C→A 4.1% A343E (GCA→GAA)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding
RA 3,758,153 C→T 2.6% A570T (GCG→ACG)  selB ← selenocysteinyl‑tRNA‑specific translation factor
MC JC 3,815,858 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,858,205 G→A 3.4% Q67* (CAG→TAG)  yidJ ← sulfatase/phosphatase superfamily protein
RA 3,858,206 G→T 3.0% N66K (AAC→AAA yidJ ← sulfatase/phosphatase superfamily protein
RA 3,858,210 G→C 4.0% A65G (GCT→GGT)  yidJ ← sulfatase/phosphatase superfamily protein
RA 3,858,212:1 +G 3.5% coding (192/1494 nt) yidJ ← sulfatase/phosphatase superfamily protein
RA 3,858,215 G→T 3.9% I63I (ATC→ATA) ‡ yidJ ← sulfatase/phosphatase superfamily protein
RA 3,858,216 A→C 3.9% I63S (ATC→AGC) ‡ yidJ ← sulfatase/phosphatase superfamily protein
RA 3,858,220 Δ1 bp 3.6% coding (184/1494 nt) yidJ ← sulfatase/phosphatase superfamily protein
RA 3,858,222 G→C 3.6% T61S (ACC→AGC)  yidJ ← sulfatase/phosphatase superfamily protein
RA 3,858,226 A→C 3.3% F60V (TTT→GTT)  yidJ ← sulfatase/phosphatase superfamily protein
RA 3,858,227 T→C 4.2% L59L (CTA→CTG yidJ ← sulfatase/phosphatase superfamily protein
MC JC 3,909,311 Δ774 bp 100% [pstA][pstC] [pstA], [pstC]
MC JC 4,001,644 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,171,647 A→C 100% noncoding (12/120 nt) rrfB → 5S ribosomal RNA of rrnB operon
RA 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,219,160 T→C 7.1% L189P (CTC→CCC)  aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,295,833 T→C 3.2% intergenic (+40/+604) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,154 T→C 14.5% intergenic (+361/+283) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,189 A→G 39.3% intergenic (+396/+248) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,190 A→C 42.8% intergenic (+397/+247) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,326,089 T→G 6.7% intergenic (‑347/+311) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4110689 4111531 843 77 [70] [73] 86 tpiA tpiA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 225468 =66 (0.380)6 (0.040) 4/490 NT 11.6% intergenic (+11/‑32) ileV/alaV tRNA‑Ile/tRNA‑Ala
?NC_000913 = 3427066 28 (0.170)intergenic (‑35/+8) alaU/ileU tRNA‑Ala/tRNA‑Ile
* ? NC_000913 = 2579070 (0.000)175 (1.040) 112/492 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
* ? NC_000913 257908 =NA (NA)175 (1.010) 95/508 NT 100% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 273955 =NA (NA)5 (0.030) 5/508 NT 3.4% intergenic (+1/+146) ykfC/insH1 CP4‑6 prophage, conserved protein,Phage or Prophage Related/IS5 transposase and trans‑activator
?NC_000913 = 700631 144 (0.830)coding (964/1221 nt) nagC N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
* ? NC_000913 607741 =113 (0.650)4 (0.020) 4/490 NT 3.5% noncoding (8/59 nt) sokE sRNA at remnant mokE/hokE locus
?NC_000913 607775 = 110 (0.660)noncoding (42/59 nt) sokE sRNA at remnant mokE/hokE locus
* ? NC_000913 = 689044NA (NA)4 (0.020) 4/506 NT 2.7% intergenic (‑31/+299) insH1/lnt IS5 transposase and trans‑activator/apolipoprotein N‑acyltransferase
?NC_000913 700627 = 147 (0.850)coding (968/1221 nt) nagC N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
* ? NC_000913 762116 =149 (0.860)6 (0.040) 3/480 NT 3.6% coding (595/1218 nt) sucB dihydrolipoyltranssuccinase
?NC_000913 762340 = 183 (1.120)coding (819/1218 nt) sucB dihydrolipoyltranssuccinase
* ? NC_000913 1207790 =110 (0.640)35 (0.220) 30/476 NT 27.7% coding (290/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 1209619 = 80 (0.490)pseudogene (1/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 = 1207805101 (0.580)50 (0.310) 39/476 NT 36.4% coding (305/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 = 1209602 80 (0.490)pseudogene (18/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 1293032 =2 (0.010)168 (0.970) 94/508 NT 98.8% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 12994980 (0.000)112 (0.660) 70/500 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1387098154 (0.890)4 (0.020) 3/496 NT 2.6% coding (379/1542 nt) tyrR aromatic amino acid biosynthesis and transport regulon transcriptional regulator, autorepressor, ATPase, phosphatase
?NC_000913 = 1387248 154 (0.910)coding (529/1542 nt) tyrR aromatic amino acid biosynthesis and transport regulon transcriptional regulator, autorepressor, ATPase, phosphatase
* ? NC_000913 1580855 =151 (0.870)8 (0.050) 4/506 NT 5.1% coding (1670/1683 nt) ydeN putative Ser‑type periplasmic non‑aryl sulfatase
?NC_000913 = 2290110 NA (NA)intergenic (‑31/+2) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 1606079 =134 (0.770)4 (0.020) 4/508 NT 2.9% intergenic (+6/‑21) lsrB/lsrF autoinducer 2‑binding protein/putative autoinducer‑2 (AI‑2) aldolase
?NC_000913 = 2070271 NA (NA)intergenic (‑91/‑6) insC1/yoeA IS2 repressor TnpA/CP4‑44 prophage, putative disrupted hemin or colicin receptor,Phage or Prophage Related, interrupted by IS2 and C‑terminal deletion
* ? NC_000913 = 1606084131 (0.760)5 (0.030) 5/506 NT 3.7% intergenic (+11/‑16) lsrB/lsrF autoinducer 2‑binding protein/putative autoinducer‑2 (AI‑2) aldolase
?NC_000913 = 1651547 NA (NA)intergenic (+10/‑4) insD1/intQ IS2 transposase TnpB,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related/pseudogene, Qin prophage, phage integrase family,Phage or Prophage Related
* ? NC_000913 = 1828681117 (0.680)4 (0.020) 3/490 NT 3.3% coding (1054/1479 nt) astD succinylglutamic semialdehyde dehydrogenase
?NC_000913 = 1828695 124 (0.740)coding (1040/1479 nt) astD succinylglutamic semialdehyde dehydrogenase
* ? NC_000913 = 19785020 (0.000)107 (0.640) 70/492 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 1979486 =0 (0.000)129 (0.750) 81/508 NT 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794890 (0.000)166 (0.960) 93/508 NT 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 2068941 =NA (NA)5 (0.030) 5/508 NT 2.5% intergenic (+1/+11) yoeA/insD1 CP4‑44 prophage, putative disrupted hemin or colicin receptor,Phage or Prophage Related, interrupted by IS2 and C‑terminal deletion/IS2 transposase TnpB
?NC_000913 2241409 = 195 (1.130)coding (258/1041 nt) galS galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor
* ? NC_000913 = 2894650174 (1.010)4 (0.020) 4/502 NT 2.6% coding (457/576 nt) ygcP putative antiterminator regulatory protein
?NC_000913 = 2894929 131 (0.770)coding (849/861 nt) ygcQ putative flavoprotein
* ? NC_000913 2996359 =NA (NA)5 (0.030) 5/508 NT 3.7% intergenic (‑1/+11) ygeO/insD1 pseudogene, orgA family, part of T3SS PAI ETT2 remnant/IS2 transposase TnpB
?NC_000913 3368707 = 130 (0.750)coding (883/1128 nt) yhcG DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
* ? NC_000913 = 3220244178 (1.030)5 (0.030) 5/488 NT 2.7% coding (753/1380 nt) patA putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
?NC_000913 = 3220396 188 (1.130)coding (905/1380 nt) patA putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
* ? NC_000913 3270591 =93 (0.540)3 (0.020) 3/492 NT 3.3% intergenic (‑1/+32) rnpB/garK RNase P, M1 RNA component/glycerate kinase I
?NC_000913 3270619 = 85 (0.510)intergenic (‑29/+4) rnpB/garK RNase P, M1 RNA component/glycerate kinase I
* ? NC_000913 = 3381845157 (0.910)9 (0.050) 8/490 NT 5.6% coding (1105/1368 nt) degQ serine endoprotease, periplasmic
?NC_000913 = 3381857 153 (0.920)coding (1117/1368 nt) degQ serine endoprotease, periplasmic
* ? NC_000913 4295831 =NA (NA)5 (0.030) 5/430 NT NA intergenic (+38/+606) gltP/yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
?NC_000913 = 4296395 NA (NA)noncoding (562/602 nt) RIP321 (repetitive extragenic palindromic) element, contains 11 REP sequences and 4 IHF sites RIP321 (repetitive extragenic palindromic) element, contains 11 REP sequences and 4 IHF sites