breseq version 0.32.0 revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 1,020,956 | A→G | intergenic (‑37/‑182) | sulA ← / → sxy | SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor |
RA | 1,196,305 | C→T | R395C (CGT→TGT) | icd → | isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase |
MC JC | 1,411,925 | Δ23,060 bp | [ttcA]–[ttcC] | 33 genes [ttcA], intR, ydaQ, ydaC, ralR, recT, recE, racC, ydaE, kilR, sieB, ydaF, ydaG, racR, ydaS, ydaT, ydaU, ydaV, ydaW, rzpR, rzoR, trkG, ynaK, ydaY, tmpR, lomR, insH1, lomR, stfR, tfaR, pinR, ynaE, [ttcC] |
|
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
RA | 3,214,413 | A→C | I457L (ATT→CTT) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
MC JC | 3,815,858 | Δ82 bp | [rph]–[rph] | [rph], [rph] | |
JC | 4,001,644 | Δ5 bp | coding (220‑224/951 nt) | corA → | magnesium/nickel/cobalt transporter |
RA | 4,049,885 | T→C | intergenic (+134/‑13) | polA → / → spf | 5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease/Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent |
RA | 4,184,542 | C→A | P1100Q (CCG→CAG) | rpoB → | RNA polymerase, beta subunit |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1293040 | 1309404 | 16365 | 40 [0] | [0] 55 | tdk–[kch] | tdk, insZ, insZ, insZ, adhE, ychE, oppA, oppB, oppC, oppD, oppF, yciU, clsA, yciY, [kch] |
* | * | ÷ | NC_000913 | 2066268–2066612 | 2066612 | 1–345 | 21 [20] | [20] 21 | [insH1] | [insH1] |
* | * | ÷ | NC_000913 | 3423734–3424527 | 3424527 | 1–794 | 21 [20] | [20] 21 | [rrfD]–[rrlD] | [rrfD], [rrlD] |
* | * | ÷ | NC_000913 | 4233736 | 4235424 | 1689 | 21 [20] | [20] 21 | pgi | pgi |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 257908 = | NA (NA) | 40 (0.810) | 34/284 | 0.4 | 100% | intergenic (+9/‑768) | crl/crl | pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers |
? | NC_000913 | = 1293039 | 0 (0.000) | intergenic (‑117/‑488) | hns/tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase | |||||
* | ? | NC_000913 | = 258675 | NA (NA) | 55 (1.110) | 47/284 | 0.0 | 100% | intergenic (+776/‑1) | crl/crl | pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers |
? | NC_000913 | 1309405 = | 0 (0.000) | coding (865/1254 nt) | kch | voltage‑gated potassium channel | |||||
* | ? | NC_000913 | 458787 = | 0 (0.000) | 54 (1.120) | 43/278 | 0.1 | 100% | intergenic (+87/‑101) | clpX/lon | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La |
? | NC_000913 | 608011 = | NA (NA) | intergenic (+23/‑54) | hokE/insL1 | toxic polypeptide, small/IS186 transposase | |||||
* | ? | NC_000913 | = 458799 | 1 (0.020) | 43 (0.900) | 33/276 | 0.4 | 97.8% | intergenic (+99/‑89) | clpX/lon | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La |
? | NC_000913 | = 609347 | NA (NA) | intergenic (+170/+112) | insL1/entD | IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex | |||||
* | ? | NC_000913 | 1207790 = | 5 (0.100) | 39 (0.890) | 31/252 | 0.3 | 86.2% | coding (290/630 nt) | stfP | e14 prophage, uncharacterized protein |
? | NC_000913 | 1209619 = | 8 (0.180) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 5 (0.100) | 33 (0.750) | 30/252 | 0.4 | 84.2% | coding (305/630 nt) | stfP | e14 prophage, uncharacterized protein |
? | NC_000913 | = 1209602 | 8 (0.180) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related | |||||
* | ? | NC_000913 | 1979486 = | 4 (0.080) | 74 (1.500) | 56/284 | 0.0 | 94.9% | intergenic (‑271/‑267) | insA/uspC | IS1 repressor TnpA/universal stress protein |
? | NC_000913 | 2101749 = | NA (NA) | intergenic (‑5/+146) | wbbL/insH1 | pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator | |||||
* | ? | NC_000913 | = 1979489 | 4 (0.080) | 69 (1.400) | 42/284 | 0.1 | 94.5% | intergenic (‑274/‑264) | insA/uspC | IS1 repressor TnpA/universal stress protein |
? | NC_000913 | = 2290111 | NA (NA) | intergenic (‑32/+1) | insH1/yejO | IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system |