breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 107,554 G→T 13.1% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
RA 151,519 A→C 5.0% I27M (ATT→ATG yadK ← putative fimbrial‑like adhesin protein
RA 164,581 A→C 21.5% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 164,620 C→T 5.4% intergenic (+86/‑110) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 164,621 A→G 5.6% intergenic (+87/‑109) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 238,535 G→T 6.0% H68N (CAT→AAT)  ykfM ← lethality reduction protein, putative inner membrane protein
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 357,775 A→T 7.0% intergenic (+321/+16) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 357,778 C→T 6.5% intergenic (+324/+13) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 777,208 A→C 9.9% K289N (AAA→AAC tolA → membrane anchored protein in TolA‑TolQ‑TolR complex
RA 780,841:1 +T 7.0% intergenic (+1/‑2) valT → / → lysW tRNA‑Val/tRNA‑Lys
RA 781,238 T→A 5.3% intergenic (+16/‑131) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 847,214 T→A 5.2% intergenic (‑95/+44) glnP ← / ← glnH glutamine transporter subunit/glutamine transporter subunit
RA 1,018,986 A→T 5.2% intergenic (+49/+27) matP → / ← ompA Ter macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)
RA 1,169,380 T→C 5.3% intergenic (+50/+32) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
RA 1,169,382 C→G 5.5% intergenic (+52/+30) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
RA 1,169,384 G→A 5.2% intergenic (+54/+28) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
RA 1,226,115 A→C 6.9% intergenic (‑35/‑485) minC ← / → ycgJ inhibitor of FtsZ ring polymerization/uncharacterized protein
RA 1,287,149 G→A 7.2% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein
RA 1,311,830 A→G 5.2% intergenic (+22/+18) tonB → / ← yciA membrane spanning protein in TonB‑ExbB‑ExbD transport complex/acyl‑CoA esterase
RA 1,311,831 C→T 5.3% intergenic (+23/+17) tonB → / ← yciA membrane spanning protein in TonB‑ExbB‑ExbD transport complex/acyl‑CoA esterase
RA 1,315,800 A→G 5.3% intergenic (‑30/+56) yciF ← / ← yciG putative rubrerythrin/ferritin‑like metal‑binding protein/KGG family protein
RA 1,315,803 C→T 5.7% intergenic (‑33/+53) yciF ← / ← yciG putative rubrerythrin/ferritin‑like metal‑binding protein/KGG family protein
RA 1,315,807 C→T 5.3% intergenic (‑37/+49) yciF ← / ← yciG putative rubrerythrin/ferritin‑like metal‑binding protein/KGG family protein
RA 1,351,228 G→C 10.1% intergenic (‑189/+179) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
RA 1,388,283 C→T 5.1% intergenic (+22/+22) tyrR → / ← tpx aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase
RA 1,388,285 A→G 5.2% intergenic (+24/+20) tyrR → / ← tpx aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase
RA 1,466,438 T→G 5.7% pseudogene (1047/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,604 G→A 8.4% pseudogene (1213/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,630 G→C 9.6% pseudogene (1239/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,639 C→T 10.1% pseudogene (1248/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,708,709 T→A 13.1% R597R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,844 C→G 6.2% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,720,364 G→T 9.6% intergenic (+21/+26) slyB → / ← slyA outer membrane lipoprotein/global transcriptional regulator
RA 1,720,367 A→C 5.6% intergenic (+24/+23) slyB → / ← slyA outer membrane lipoprotein/global transcriptional regulator
RA 1,810,062 T→C 5.1% intergenic (+14/‑149) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
RA 1,810,066 C→A 5.3% intergenic (+18/‑145) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
RA 1,810,069 G→A 5.0% intergenic (+21/‑142) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
RA 1,864,758 G→A 7.7% intergenic (+24/+24) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,864,763 A→T 7.7% intergenic (+29/+19) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,864,768 T→C 7.8% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,865,682 G→C 5.7% intergenic (‑46/+44) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein
RA 2,062,354 T→A 5.1% intergenic (+19/+37) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
RA 2,062,355 T→G 5.1% intergenic (+20/+36) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
RA 2,062,356 C→A 5.0% intergenic (+21/+35) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
RA 2,062,357 T→A 5.1% intergenic (+22/+34) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
RA 2,068,407 A→G 7.2% pseudogene (13/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,068,408 T→A 7.6% pseudogene (12/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,068,409 G→C 7.2% pseudogene (11/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,068,410 T→A 7.0% pseudogene (10/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,068,411 C→T 7.1% pseudogene (9/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,177,234 C→A 10.7% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,177,245 G→T 9.3% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,229,177 A→C 15.3% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C
RA 2,304,649 C→T 14.7% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,988 T→C 5.6% intergenic (+595/+120) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
JC JC 2,375,828 IS186 (+) +4 bp 100% coding (132‑135/963 nt) menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,432,985 A→G 6.7% intergenic (‑43/+27) folC ← / ← accD bifunctional folylpolyglutamate synthase/ dihydrofolate synthase/acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit
RA 2,432,990 G→C 6.7% intergenic (‑48/+22) folC ← / ← accD bifunctional folylpolyglutamate synthase/ dihydrofolate synthase/acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit
RA 2,470,431 G→T 13.0% R434I (AGA→ATA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,817,916 T→G 7.8% intergenic (‑56/+143) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,818,046 A→G 8.1% intergenic (‑186/+13) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,818,049 T→A 9.8% intergenic (‑189/+10) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 3,046,050 T→G 9.0% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
RA 3,239,630 A→T 13.6% intergenic (+85/‑314) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,269,956 T→C 5.5% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,270,082 A→C 7.0% intergenic (+480/+134) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,504,873 T→A 9.6% intergenic (+90/+62) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,608,695 C→G 10.3% intergenic (+46/+56) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,608,696 T→G 5.6% intergenic (+47/+55) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,608,721 A→C 9.8% intergenic (+72/+30) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,728,109 A→C 8.2% D421E (GAT→GAG xylB ← xylulokinase
RA 3,781,051 C→G 7.3% intergenic (+34/‑164) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,815,809 Δ1 bp 15.3% intergenic (‑41/+25) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,877,558 G→C 5.6% intergenic (‑93/+147) yidB ← / ← gyrB DUF937 family protein/DNA gyrase, subunit B
JC JC 3,890,075 IS5 (–) +4 bp 100% coding (1346‑1349/1416 nt) tnaA → tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,959,457 T→G 7.0% intergenic (+12/+75) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,959,482 A→C 7.3% intergenic (+37/+50) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,959,501 T→A 14.2% intergenic (+56/+31) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 4,051,259 G→T 5.3% noncoding (224/245 nt) csrC → CsrC sRNA sequesters CsrA, a carbon flux regulator
RA 4,051,262 A→C 5.1% noncoding (227/245 nt) csrC → CsrC sRNA sequesters CsrA, a carbon flux regulator
RA 4,060,261 G→T 5.6% intergenic (+31/‑186) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator
RA 4,060,264 A→C 6.0% intergenic (+34/‑183) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator
RA 4,153,447 A→G 6.2% intergenic (‑349/+249) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,177,171 T→A 5.1% intergenic (+43/‑187) tufB → / → secE translation elongation factor EF‑Tu 2/preprotein translocase membrane subunit
RA 4,182,820 C→T 6.5% H526Y (CAC→TAC)  rpoB → RNA polymerase, beta subunit
RA 4,185,540 C→T 38.1% P64L (CCG→CTG)  rpoC → RNA polymerase, beta prime subunit
RA 4,187,214 A→G 41.2% D622G (GAC→GGC)  rpoC → RNA polymerase, beta prime subunit
RA 4,296,060 C→T 32.6% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,155 T→C 8.0% intergenic (+361/+281) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,325,948 A→G 6.3% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,392,582 A→C 7.0% intergenic (+35/‑1) glyX → / → glyY tRNA‑Gly/tRNA‑Gly
RA 4,406,129 T→C 100% intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
JC JC 4,640,551 IS1 (–) +9 bp 100% intergenic (+9/‑383) yjjY → / → yjtD uncharacterized protein/putative methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 772987 774218 1232 119 [117] [115] 119 [cydA]–[cydX] [cydA],cydB,[cydX]
* * ÷ NC_000913 1037678 1039332 1655 119 [114] [116] 119 cbdA–[cbdB] cbdA,[cbdB]
* * ÷ NC_000913 2403898 2404685 788 122 [117] [115] 120 nuoB–[nuoA] nuoB,[nuoA]
* * ÷ NC_000913 3423752–3424547 3424547 1–796 119 [118] [114] 120 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 16658NA (NA)3 (0.010) 3/268 NT NA noncoding (1272/1345 nt) IS186 repeat region
?NC_000913 = 16686 NA (NA)noncoding (1300/1345 nt) IS186 repeat region
* ? NC_000913 = 228108NA (NA)8 (0.030) 4/268 NT NA noncoding (2350/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 = 228124 NA (NA)noncoding (2366/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
* ? NC_000913 228359 =NA (NA)4 (0.020) 3/264 NT NA noncoding (2601/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 228397 = NA (NA)noncoding (2639/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
* ? NC_000913 274620 =NA (NA)8 (0.040) 4/258 NT NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 = 315857NA (NA)9 (0.040) 5/270 NT NA noncoding (629/1255 nt) IS3 repeat region
?NC_000913 = 315870 NA (NA)noncoding (642/1255 nt) IS3 repeat region
* ? NC_000913 = 381669NA (NA)6 (0.030) 4/262 NT NA noncoding (410/1331 nt) IS2 repeat region
?NC_000913 = 381695 NA (NA)noncoding (436/1331 nt) IS2 repeat region
* ? NC_000913 = 381910NA (NA)13 (0.060) 5/264 NT NA noncoding (651/1331 nt) IS2 repeat region
?NC_000913 = 381923 NA (NA)noncoding (664/1331 nt) IS2 repeat region
* ? NC_000913 770669 =NA (NA)8 (0.030) 5/270 NT NA intergenic (+58/‑789) mngB/cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
?NC_000913 = 832368 NA (NA)noncoding (80/98 nt) RIP75 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP75 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 781369 =193 (0.790)31 (0.130)
+GAAG
13/268 NT 15.6% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 152 (0.630)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 1207790 =110 (0.450)85 (0.400) 52/244 NT 41.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 138 (0.640)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1207805103 (0.420)82 (0.380) 48/244 NT 41.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 138 (0.640)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)184 (0.780) 103/268 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 2729475 =109 (0.450)6 (0.030) 4/256 NT 5.1% intergenic (‑31/+141) gltW/rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
?NC_000913 = 4168363 121 (0.540)intergenic (+163/‑9) rrsB/gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu
* ? NC_000913 = 2817942201 (0.830)17 (0.080) 13/236 NT 8.9% intergenic (‑82/+117) argQ/argZ tRNA‑Arg/tRNA‑Arg
?NC_000913 2818492 = 178 (0.860)noncoding (58/77 nt) argV tRNA‑Arg
* ? NC_000913 = 3255287NA (NA)3 (0.010) 3/264 NT NA noncoding (71/103 nt) REP236 (repetitive extragenic palindromic) element; contains 2 REP sequences REP236 (repetitive extragenic palindromic) element; contains 2 REP sequences
?NC_000913 = 3255319 NA (NA)noncoding (103/103 nt) REP236 (repetitive extragenic palindromic) element; contains 2 REP sequences REP236 (repetitive extragenic palindromic) element; contains 2 REP sequences