breseq  version 0.35.4  revision f352f80f4bc9 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence	position	mutation	annotation	gene	description
RA	123,050	A→T	I12F (ATC→TTC)	aceE →	pyruvate dehydrogenase E1 component
RA	125,085	2 bp→CA	coding (2069‑2070/2664 nt)	aceE →	pyruvate dehydrogenase E1 component
MC JC	257,908	Δ776 bp		insB9–[crl]	insB9, insA9, [crl]
JC JC	568,268	IS1 (–) +9 bp	intergenic (+21/‑39)	renD → / → emrE	DLP12 prophage; protein RenD/multidrug/betaine/choline efflux transporter EmrE
RA	1,196,220	C→T	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase
RA	1,196,232	C→T	T370T (ACC→ACT)	icd →	isocitrate dehydrogenase
JC JC	1,293,032	IS1 (–) +8 bp	intergenic (‑110/‑488)	hns ← / → tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC	1,299,499	Δ1,199 bp		insH21	insH21
RA	1,934,850	C→A	E489* (GAG→TAG)	zwf ←	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
MC JC	1,978,503	Δ776 bp		insB‑5–insA‑5	insB‑5, insA‑5
JC JC	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA‑5 ← / → uspC	IS1 protein InsA/universal stress protein C
RA	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative colanic acid biosynthesis glycosyl transferase
RA	2,173,363	Δ2 bp	pseudogene (915‑916/1358 nt)	gatC ←	galactitol‑specific PTS enzyme IIC component
RA	2,447,639	G→A	H268Y (CAT→TAT)	prmB ←	50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase
RA	2,512,749	G→A	intergenic (‑43/‑293)	mntH ← / → nupC	Mn(2(+))/Fe(2(+)): H(+) symporter MntH/nucleoside:H(+) symporter NupC
RA	2,661,840	A→C	C98G (TGC→GGC)	iscR ←	DNA‑binding transcriptional dual regulator IscR
RA	2,917,603	T→G	V849V (GTT→GTG)	barA →	sensory histidine kinase BarA
RA	3,041,398	T→G	L29R (CTT→CGT)	ygfZ →	folate‑binding protein
RA	3,210,838	A→G	K20E (AAG→GAG)	rpsU →	30S ribosomal subunit protein S21
RA	3,560,455	+G	pseudogene (151/758 nt)	glpR ←	DNA‑binding transcriptional repressor GlpR
MC JC	3,815,859	Δ82 bp		[rph]	[rph]
JC	4,001,645	Δ5 bp	coding (220‑224/951 nt)	corA →	Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA	4,184,543	C→A	P1100Q (CCG→CAG)	rpoB →	RNA polymerase subunit beta
RA	4,296,381	+GC	intergenic (+587/+55)	gltP → / ← yjcO	glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1196245	1211458	15214	27 [25]	[25] 26	[icd]–[icdC]	[icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
*	*	÷	NC_000913	3423741–3424527	3424527	1–787	26 [25]	[24] 26	[rrfD]–[rrlD]	[rrfD],[rrlD]
*	*	÷	NC_000913	3621167–3622297	3653150–3652036	29740–31984	26 [25]	[25] 27	[rhsB]–[insH‑11]	[rhsB],yhhH,yhhI,yhhJ,rbbA,yhiI,yhiJ,yhiL,yhiM,yhiN,pitA,uspB,uspA,dtpB,rsmJ,prlC,rlmJ,gor,dinQ,agrA,agrB,arsR,arsB,arsC,yhiS,[insH‑11]