breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 139,433 T→A D598V (GAT→GTT)  gcd ← quinoprotein glucose dehydrogenase
RA 204,655 C→T L55L (CTG→TTG)  rnhB → RNase HII
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 781,471 C→G intergenic (+27/‑106) lysZ → / → lysQ tRNA‑Lys/tRNA‑Lys
RA 834,202 A→G D378G (GAC→GGC)  dinG → ATP‑dependent DNA helicase DinG
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,321,134 C→A C84F (TGC→TTC)  trpD ← anthranilate synthase subunit TrpD
RA 1,548,086 T→C M229T (ATG→ACG)  fdnG → formate dehydrogenase N subunit alpha
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,731,942 C→A T952T (ACC→ACA lhr → putative ATP‑dependent helicase Lhr
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,135,581 G→T V24V (GTC→GTA wzc ← protein‑tyrosine kinase Wzc
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,407,208 C→A intergenic (‑567/‑353) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
RA 2,488,147 G→T A341D (GCC→GAC)  yfdE ← acetyl‑CoA:oxalate CoA‑transferase
RA 2,641,632 A→G I58T (ATC→ACC)  ispG ← (E)‑4‑hydroxy‑3‑methylbut‑2‑enyl‑diphosphate synthase (flavodoxin)
RA 2,824,433 G→T intergenic (‑87/+58) pncC ← / ← mltB NMN aminohydrolase/membrane‑bound lytic murein transglycosylase B
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,386,412 G→T R593S (CGC→AGC)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,543,860 G→T V232F (GTT→TTT)  rpnA → recombination‑promoting nuclease RpnA
RA 3,556,470 G→T P127Q (CCG→CAG)  rtcA ← RNA 3'‑terminal phosphate cyclase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,580,336 C→A R157L (CGC→CTC)  yhhX ← putative oxidoreductase YhhX
RA 3,580,996 C→A noncoding (21/90 nt) ryhB ← small regulatory RNA RyhB
RA 3,688,254 C→A G307V (GGG→GTG)  bcsC ← cellulose biosynthesis protein BcsC
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,867,114 C→A E103D (GAG→GAT ibpA ← small heat shock protein IbpA
RA 3,942,018 G→T noncoding (211/1542 nt) rrsC → 16S ribosomal RNA
RA 4,031,174 C→A A5D (GCC→GAC)  pepQ → Xaa‑Pro dipeptidase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,340,227 C→A A99S (GCC→TCC)  adiA ← arginine decarboxylase, degradative
RA 4,345,861 G→T A489E (GCG→GAG)  fumB ← fumarase B
RA 4,394,055 G→T L5I (CTC→ATC)  queG ← epoxyqueuosine reductase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2520959 2521091 133 42 [41] [39] 42 [valU]–[valX] [valU],[valX]
* * ÷ NC_000913 3102950 3104082 1133 42 [41] [40] 42 mutY mutY
* * ÷ NC_000913 3423746–3424234 3424545–3424238 5–800 42 [38] [39] 46 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)165 (1.290) 92/270 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 2101745 =NA (NA)94 (0.710) 53/278 0.9 NA intergenic (‑1/+150) wbbL/insH‑7 interrupted rhamnosyltransferase WbbL/IS5 transposase and trans‑activator
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 3363001 =166 (1.260)188 (1.430) 83/276 0.1 68.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 8 (0.060)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)154 (1.170) 81/278 0.1 95.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 8 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA